Restriction map for
pFN21ASDA0205
(Circular) (Six-base) MAPSORT of: pFN21ASDA0205.seq Check: 5422 from: 1 to: 5839
pFN21ASDA0205 5839 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 3902 5286 5554 1632
Size: 503 1767 1384 268 1917
Fragments arranged by size:
1917 1767 1384 503 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 2863 4738 5715 1203
Size: 1660 1875 977 1327
Fragments arranged by size:
1875 1660 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2503 3582 5154 2503
Size: 1079 1572 3188
Fragments arranged by size:
3188 1572 1079
ApaLI G'TGCA_C
Cuts at: 2344 3896 5052 2344
Size: 1552 1156 3131
Fragments arranged by size:
3131 1552 1156
ApoI r'AATT_y
Cuts at: 1493 3148 3374 3526 5635 1493
Size: 1655 226 152 2109 1697
Fragments arranged by size:
2109 1697 1655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BamHI G'GATC_C
Cuts at: 2832 3169 3536 2832
Size: 337 367 5135
Fragments arranged by size:
5135 367 337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3158 1391
Size: 1767 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2961 928
Size: 971 1062 3806
Fragments arranged by size:
3806 1062 971
BbvCI CC'TCA_GC
Cuts at: 3038 3038
Size: 5839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2834 3731 4941 2834
Size: 897 1210 3732
Fragments arranged by size:
3732 1210 897
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 136
Size: 107 122 71 631 605 2778 1525
Fragments arranged by size:
2778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2480 5834 2480
Size: 3354 2485
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2348 3900 5056 1790
Size: 558 1552 1156 2573
Fragments arranged by size:
2573 1552 1156 558
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2753 2924 4499 1239
Size: 1109 405 171 1575 2579
Fragments arranged by size:
2579 1575 1109 405 171
Bpu10I CC'TnA_GC
Cuts at: 3038 3038
Size: 5839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 3964 4829 5127 2495
Size: 1469 865 298 3207
Fragments arranged by size:
3207 1469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3136 3630 493
Size: 711 1932 494 2702
Fragments arranged by size:
2702 1932 711 494
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2745 1186
Size: 1559 4280
BseYI C'CCAG_C
Cuts at: 2053 5042 5538 2053
Size: 2989 496 2354
Fragments arranged by size:
2989 2354 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2348 3900 3985 5056 2348
Size: 1552 85 1071 3131
Fragments arranged by size:
3131 1552 1071 85
BsmI GAATG_Cn'
Cuts at: 2695 3350 3443 2695
Size: 655 93 5091
Fragments arranged by size:
5091 655 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 3158 3900 3985 5056 1391
Size: 399 558 810 742 85 1071 2174
Fragments arranged by size:
2174 1071 810 742 558 399 85
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3121 4334 4508 65
Size: 1183 1873 1213 174 1396
Fragments arranged by size:
1873 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 2866 96
Size: 2770 3069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5839
BstBI TT'CG_AA
Cuts at: 2007 4643 2007
Size: 2636 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 2480 2832 2877 3169 3536 3921 3938 5390
Size: 508 352 45 292 367 385 17 1452
Cuts at: 5390 5476 5488 5834 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 508 385 367 352 346 292
86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2585 2585
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2022 2048 513
Size: 552 957 26 4304
Fragments arranged by size:
4304 957 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 3355 4149 4169 2313
Size: 1042 794 20 3983
Fragments arranged by size:
3983 1042 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804
Size: 54 1425 50 275 18 1456 889 1629
Cuts at: 5804 8
Size: 43
Fragments arranged by size:
1629 1456 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 3769 1353
Size: 1232 1184 3423
Fragments arranged by size:
3423 1232 1184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2348 2753 2924 3902 4499 5286
Size: 393 503 213 405 171 978 597 787
Cuts at: 5286 5554 1239
Size: 268 1524
Fragments arranged by size:
1524 978 787 597 503 405 393 268
213 171
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 5839
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 4986 1678
Size: 3308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2530 2562 2741 2773 2782
Size: 32 135 32 958 32 179 32 9
Cuts at: 2782 2814 2948 2980 4480 4512 4554 4586 1373
Size: 32 134 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 958 179 135 134 42 32
32 32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 3183 3429 669
Size: 988 281 166 1079 246 3079
Fragments arranged by size:
3079 1079 988 281 246 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4017
Fragments arranged by size:
4017 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2973 3797 4156 4315 518
Size: 648 1185 622 824 359 159 2042
Fragments arranged by size:
2042 1185 824 648 622 359 159
MspA1I CmG'CkG
Cuts at: 1606 2500 2938 3255 3932 5080 5325 1606
Size: 894 438 317 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 438 317 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4330
Fragments arranged by size:
4330 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2867 3197 4742 1132
Size: 97 1638 330 1545 2229
Fragments arranged by size:
2229 1638 1545 330 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 2863 4738 2863
Size: 1875 3964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2666 1108
Size: 1558 4281
PfoI T'CCnGG_A
Cuts at: 2364 2364
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 2468 3409 2468
Size: 941 4898
PstI C_TGCA'G
Cuts at: 830 3191 830
Size: 2361 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2500 2500
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5839
SfcI C'TryA_G
Cuts at: 826 1045 2814 3187 4322 5003 5194 826
Size: 219 1769 373 1135 681 191 1471
Fragments arranged by size:
1769 1471 1135 681 373 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 3943 4844 5106 820
Size: 197 926 567 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 567 262 197
SnaBI TAC'GTA
Cuts at: 493 3136 3630 493
Size: 2643 494 2702
Fragments arranged by size:
2702 2643 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 2766 1517
Size: 1249 4590
StyI C'CwwG_G
Cuts at: 513 1065 2022 2660 2836 3000 3278 513
Size: 552 957 638 176 164 278 3074
Fragments arranged by size:
3074 957 638 552 278 176 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 2866 4085
Size: 274 80 33 51 1443 444 445 1219
Cuts at: 4085 5797 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1219 445 444 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2244 2276 5799 5831 2244
Size: 32 3523 32 2252
Fragments arranged by size:
3523 2252 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 2440 3968 2440
Size: 1528 4311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI