Restriction map for
pFN21ASDA0201
(Circular) (Six-base) MAPSORT of: pFN21ASDA0201.seq Check: 7347 from: 1 to: 7303
pFN21ASDA0201 7303 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5113 278
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4646 1937
Size: 2709 4594
Acc65I G'GTAC_C
Cuts at: 4624 4624
Size: 7303
AclI AA'CG_TT
Cuts at: 1563 5430 5803 1563
Size: 3867 373 3063
Fragments arranged by size:
3867 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 3282 3573 4059 4170 4204 5366 6750
Size: 785 865 291 486 111 34 1162 1384
Cuts at: 6750 7018 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1162 865 785 486 291 268
111 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7106
AflIII A'CryG_T
Cuts at: 1203 3858 4406 6202 7179 1203
Size: 2655 548 1796 977 1327
Fragments arranged by size:
2655 1796 1327 977 548
AgeI A'CCGG_T
Cuts at: 2118 6134 2118
Size: 4016 3287
AhdI GACnn_n'nnGTC
Cuts at: 6032 6032
Size: 7303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4215 1184
Size: 3031 4272
AlwNI CAG_nnn'CTG
Cuts at: 2474 3602 3731 4037 4189 5046 6618 2474
Size: 1128 129 306 152 857 1572 3159
Fragments arranged by size:
3159 1572 1128 857 306 152 129
ApaLI G'TGCA_C
Cuts at: 2122 5360 6516 2122
Size: 3238 1156 2909
Fragments arranged by size:
3238 2909 1156
ApoI r'AATT_y
Cuts at: 1493 2568 2762 3060 3089 3194 3986 4012 4573
Size: 1075 194 298 29 105 792 26 561
Cuts at: 4573 4612 4838 4990 7099 1493
Size: 39 226 152 2109 1697
Fragments arranged by size:
2109 1697 1075 792 561 298 226 194
152 105 39 29 26
AseI AT'TA_AT
Cuts at: 160 1032 4106 5857 160
Size: 872 3074 1751 1606
Fragments arranged by size:
3074 1751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 4628 1870
Size: 73 133 2552 4545
Fragments arranged by size:
4545 2552 133 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2804 2837 2804
Size: 33 7270
BamHI G'GATC_C
Cuts at: 4351 4633 5000 4351
Size: 282 367 6654
Fragments arranged by size:
6654 367 282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4624 6079 618
Size: 325 217 627 2837 1455 1842
Fragments arranged by size:
2837 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2858 4622 1391
Size: 660 807 1764 4072
Fragments arranged by size:
4072 1764 807 660
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3540 4047 4515 928
Size: 971 1641 507 468 3716
Fragments arranged by size:
3716 1641 971 507 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5494 5528 1903
Size: 34 3557 34 3678
Fragments arranged by size:
3678 3557 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 4345 1548
Size: 2797 4506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5914 136
Size: 107 122 71 631 605 4242 1525
Fragments arranged by size:
4242 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7298 7298
Size: 7303
BlpI GC'TnA_GC
Cuts at: 4720 4720
Size: 7303
Bme1580I G_kGCm'C
Cuts at: 1790 2126 5364 6520 1790
Size: 336 3238 1156 2573
Fragments arranged by size:
3238 2573 1156 336
BmgBI CAC'GTC
Cuts at: 1318 2044 1318
Size: 726 6577
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2699 5034 5992
Size: 416 53 83 399 24 1282 2335 958
Cuts at: 5992 442
Size: 1753
Fragments arranged by size:
2335 1753 1282 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3750 4107 5963 1239
Size: 2511 357 1856 2579
Fragments arranged by size:
2579 2511 1856 357
Bpu10I CC'TnA_GC
Cuts at: 4326 4368 4326
Size: 42 7261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2302 2405 5428 6293 6591 2302
Size: 103 3023 865 298 3014
Fragments arranged by size:
3023 3014 865 298 103
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 3574 5966 882
Size: 617 588 1487 2392 2219
Fragments arranged by size:
2392 2219 1487 617 588
BsaAI yAC'GTr
Cuts at: 493 1204 4364 4600 5094 493
Size: 711 3160 236 494 2702
Fragments arranged by size:
3160 2702 711 494 236
BsaBI GATnn'nnATC
Cuts at: 4350 7297 4350
Size: 2947 4356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5110 5492 6127 275
Size: 53 83 186 936 3577 382 635 1451
Fragments arranged by size:
3577 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 2915 5736 6134 6408 6555 6966 2118
Size: 797 2821 398 274 147 411 2455
Fragments arranged by size:
2821 2455 797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2793 2823 1178
Size: 30 165 30 1390 30 5658
Fragments arranged by size:
5658 1390 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3548 4016 1186
Size: 2362 468 4473
Fragments arranged by size:
4473 2362 468
BseYI C'CCAG_C
Cuts at: 6506 7002 6506
Size: 496 6807
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2484 2778 3051 3411 1163
Size: 573 748 294 273 360 5055
Fragments arranged by size:
5055 748 573 360 294 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2119 4753 5514 6542 1540
Size: 275 186 118 2634 761 1028 2301
Fragments arranged by size:
2634 2301 1028 761 275 186 118
BsiHKAI G_wGCw'C
Cuts at: 2126 2809 2858 4369 5364 5449 6520 2126
Size: 683 49 1511 995 85 1071 2909
Fragments arranged by size:
2909 1511 1071 995 683 85 49
BsmI GAATG_Cn'
Cuts at: 2243 2894 4327 4814 4907 2243
Size: 651 1433 487 93 4639
Fragments arranged by size:
4639 1433 651 487 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2126 2809 2858 4369 4622 5364
Size: 399 261 75 683 49 1511 253 742
Cuts at: 5364 5449 6520 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1511 1071 742 683 399 261 253
85 75 49
BspEI T'CCGG_A
Cuts at: 2915 6966 2915
Size: 4051 3252
BspHI T'CATG_A
Cuts at: 3271 4317 5192 6922 3271
Size: 1046 875 1730 3652
Fragments arranged by size:
3652 1730 1046 875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4658 844
Size: 3814 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5190 1394
Size: 429 3367 3507
Fragments arranged by size:
3507 3367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5798 5972 65
Size: 1183 4550 174 1396
Fragments arranged by size:
4550 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 5947 6134 1700
Size: 110 308 3829 187 2869
Fragments arranged by size:
3829 2869 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 2334 96
Size: 2238 5065
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7303
BssSI C'ACGA_G
Cuts at: 5363 6375 5363
Size: 1012 6291
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7303
BstBI TT'CG_AA
Cuts at: 2007 6107 2007
Size: 4100 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7303
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 7303
BstYI r'GATC_y
Cuts at: 1972 2581 4351 4633 5000 5385 5402 6854 6940
Size: 609 1770 282 367 385 17 1452 86
Cuts at: 6940 6952 7298 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1770 1452 609 385 367 346 282
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3481 513
Size: 552 2416 4335
Fragments arranged by size:
4335 2416 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2050 2083 487
Size: 683 56 824 33 5707
Fragments arranged by size:
5707 824 683 56 33
BtsI GCAGTG_nn'
Cuts at: 4819 5613 5633 4819
Size: 794 20 6489
Fragments arranged by size:
6489 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3952 3987 529
Size: 35 3388 35 3845
Fragments arranged by size:
3845 3388 35 35
DraI TTT'AAA
Cuts at: 2767 3184 4607 2767
Size: 417 1423 5463
Fragments arranged by size:
5463 1423 417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6310 809
Size: 507 4994 1802
Fragments arranged by size:
4994 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 4157 4750
Size: 54 1425 50 275 18 406 1921 593
Cuts at: 4750 5639 7268 8
Size: 889 1629 43
Fragments arranged by size:
1921 1629 1425 889 593 406 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4750 1537
Size: 275 2938 4090
Fragments arranged by size:
4090 2938 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5233 1353
Size: 3880 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5890 6264 6410 387
Size: 1064 138 4301 374 146 1280
Fragments arranged by size:
4301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2856 2856
Size: 7303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 3282 3573 3750 4059 4107 4170
Size: 393 785 865 291 177 309 48 63
Cuts at: 4170 4204 5366 5963 6750 7018 1239
Size: 34 1162 597 787 268 1524
Fragments arranged by size:
1524 1162 865 787 785 597 393 309
291 268 177 63 48 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2712 2712
Size: 7303
EcoO109I rG'GnC_Cy
Cuts at: 3335 3335
Size: 7303
EcoRI G'AATT_C
Cuts at: 3060 4612 4990 3060
Size: 1552 378 5373
Fragments arranged by size:
5373 1552 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2999 3354 3869 2296
Size: 703 355 515 5730
Fragments arranged by size:
5730 703 515 355
FspI TGC'GCA
Cuts at: 5809 5809
Size: 7303
HaeII r_GCGC'y
Cuts at: 1678 3054 6450 1678
Size: 1376 3396 2531
Fragments arranged by size:
3396 2531 1376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2468 2500 2777
Size: 32 135 32 461 32 403 32 277
Cuts at: 2777 2809 3444 3476 4330 4362 5944 5976 6018
Size: 32 635 32 854 32 1582 32 42
Cuts at: 6018 6050 1373
Size: 32 2626
Fragments arranged by size:
2626 1582 854 635 461 403 277 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 3475 3757 4090 4647 4893
Size: 988 281 450 1087 282 333 557 246
Cuts at: 4893 669
Size: 3079
Fragments arranged by size:
3079 1087 988 557 450 333 282 281
246
HindIII A'AGCT_T
Cuts at: 748 3351 748
Size: 2603 4700
HpaI GTT'AAC
Cuts at: 4893 4893
Size: 7303
KpnI G_GTAC'C
Cuts at: 4628 4628
Size: 7303
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 7179 7179
Size: 7303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 2299 2763 2976 3025 4077 6417 6601
Size: 714 280 464 213 49 1052 2340 184
Cuts at: 6601 1305
Size: 2007
Fragments arranged by size:
2340 2007 1052 714 464 280 213 184
49
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5481
Fragments arranged by size:
5481 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2754 3157 4251 4545 5261 5620
Size: 648 861 727 403 1094 294 716 359
Cuts at: 5620 5779 518
Size: 159 2042
Fragments arranged by size:
2042 1094 861 727 716 648 403 359
294 159
MspA1I CmG'CkG
Cuts at: 1606 2549 3068 4214 4719 5396 6544 6789 1606
Size: 943 519 1146 505 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 943 677 519 505 245
NcoI C'CATG_G
Cuts at: 513 1065 3481 513
Size: 552 2416 4335
Fragments arranged by size:
4335 2416 552
NdeI CA'TA_TG
Cuts at: 387 3979 4084 387
Size: 3592 105 3606
Fragments arranged by size:
3606 3592 105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7303
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 3862 4410 4661 6206 1132
Size: 97 2633 548 251 1545 2229
Fragments arranged by size:
2633 2229 1545 548 251 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7303
PciI A'CATG_T
Cuts at: 3858 4406 6202 3858
Size: 548 1796 4959
Fragments arranged by size:
4959 1796 548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7303
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 6885 6917 5372
Size: 32 1481 32 5758
Fragments arranged by size:
5758 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7303
PsiI TTA'TAA
Cuts at: 2574 4873 2574
Size: 2299 5004
PstI C_TGCA'G
Cuts at: 830 4194 4655 830
Size: 3364 461 3478
Fragments arranged by size:
3478 3364 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
PvuII CAG'CTG
Cuts at: 2549 3068 4214 2549
Size: 519 1146 5638
Fragments arranged by size:
5638 1146 519
SacI G_AGCT'C
Cuts at: 2858 2858
Size: 7303
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 5551 7263 5551
Size: 1712 5591
SfcI C'TryA_G
Cuts at: 826 1045 4190 4651 5786 6467 6658 826
Size: 219 3145 461 1135 681 191 1471
Fragments arranged by size:
3145 1471 1135 681 461 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 2078 4630 1872
Size: 206 2552 4545
Fragments arranged by size:
4545 2552 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2281 2420 5407 6308 6570 820
Size: 197 926 338 139 2987 901 262 1553
Fragments arranged by size:
2987 1553 926 901 338 262 197 139
SnaBI TAC'GTA
Cuts at: 493 4600 5094 493
Size: 4107 494 2702
Fragments arranged by size:
4107 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7303
SphI G_CATG'C
Cuts at: 1132 4661 1132
Size: 3529 3774
SrfI GCCC'GGGC
Cuts at: 2078 2078
Size: 7303
SspI AAT'ATT
Cuts at: 5 52 4483 5227 5
Size: 47 4431 744 2081
Fragments arranged by size:
4431 2081 744 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7303
StyI C'CwwG_G
Cuts at: 513 1065 3370 3481 4742 513
Size: 552 2305 111 1261 3074
Fragments arranged by size:
3074 2305 1261 552 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 3494 4465 5330 5347 5500 5685
Size: 1255 750 1164 971 865 17 153 185
Cuts at: 5685 6130 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1164 971 865 750 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 2775 5549
Size: 274 80 33 51 1443 357 441 2774
Cuts at: 5549 7261 96
Size: 1712 138
Fragments arranged by size:
2774 1712 1443 441 357 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2857 2889 7263 7295 2857
Size: 32 4374 32 2865
Fragments arranged by size:
4374 2865 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7303
XbaI T'CTAG_A
Cuts at: 3449 4639 3449
Size: 1190 6113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 3790 2029
Size: 1761 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7303
XmaI C'CCGG_G
Cuts at: 1870 2076 4628 1870
Size: 206 2552 4545
Fragments arranged by size:
4545 2552 206
XmnI GAAnn'nnTTC
Cuts at: 3267 5432 3267
Size: 2165 5138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5111 276
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SwaI