Restriction map for
pFN21ASDA0198
(Circular) (Six-base) MAPSORT of: pFN21ASDA0198.seq Check: 200 from: 1 to: 6217
pFN21ASDA0198 6217 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 4280 5664 5932 1632
Size: 935 1713 1384 268 1917
Fragments arranged by size:
1917 1713 1384 935 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 2726 2787 5116 6093 1203
Size: 1523 61 2329 977 1327
Fragments arranged by size:
2329 1523 1327 977 61
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 2598 2948 1166
Size: 1432 350 4435
Fragments arranged by size:
4435 1432 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 3500 1184
Size: 1782 534 3901
Fragments arranged by size:
3901 1782 534
AlwNI CAG_nnn'CTG
Cuts at: 2533 2758 3367 3410 3960 5532 2533
Size: 225 609 43 550 1572 3218
Fragments arranged by size:
3218 1572 609 550 225 43
ApaLI G'TGCA_C
Cuts at: 2657 2966 4274 5430 2657
Size: 309 1308 1156 3444
Fragments arranged by size:
3444 1308 1156 309
ApoI r'AATT_y
Cuts at: 1493 2151 3526 3752 3904 6013 1493
Size: 658 1375 226 152 2109 1697
Fragments arranged by size:
2109 1697 1375 658 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2862 3542 1870
Size: 73 166 753 680 4545
Fragments arranged by size:
4545 753 680 166 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2451 2576 2646 2918 3538
Size: 325 217 627 664 125 70 272 620
Cuts at: 3538 4993 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 664 627 620 325 272 217
125 70
BanII G_rGCy'C
Cuts at: 1391 2781 2843 3221 3536 1391
Size: 1390 62 378 315 4072
Fragments arranged by size:
4072 1390 378 315 62
BbeI G_GCGC'C
Cuts at: 2455 2455
Size: 6217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3448 928
Size: 971 1549 3697
Fragments arranged by size:
3697 1549 971
BbvCI CC'TCA_GC
Cuts at: 2186 2235 2489 3168 3354 2186
Size: 49 254 679 186 5049
Fragments arranged by size:
5049 679 254 186 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BfrBI ATG'CAT
Cuts at: 2467 2467
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4828 136
Size: 107 122 71 631 605 3156 1525
Fragments arranged by size:
3156 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2837 3030 3387 3634 2837
Size: 193 357 247 5420
Fragments arranged by size:
5420 357 247 193
Bme1580I G_kGCm'C
Cuts at: 1790 2661 2698 2923 2970 4278 5434 1790
Size: 871 37 225 47 1308 1156 2573
Fragments arranged by size:
2573 1308 1156 871 225 47 37
BmgBI CAC'GTC
Cuts at: 1318 3462 1318
Size: 2144 4073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3284 3948 4906
Size: 416 53 83 399 24 1867 664 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1867 1753 958 664 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3015 3015
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4877 1239
Size: 3638 2579
Bpu10I CC'TnA_GC
Cuts at: 2186 2235 2489 3168 3354 2186
Size: 49 254 679 186 5049
Fragments arranged by size:
5049 679 254 186 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3378 4342 5207 5505 2747
Size: 631 964 865 298 3459
Fragments arranged by size:
3459 964 865 631 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2212 4880 882
Size: 617 713 2668 2219
Fragments arranged by size:
2668 2219 713 617
BsaAI yAC'GTr
Cuts at: 493 1204 3514 4008 493
Size: 711 2310 494 2702
Fragments arranged by size:
2702 2310 711 494
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2452 4024 4406 5041
Size: 53 83 186 936 919 1572 382 635
Cuts at: 5041 275
Size: 1451
Fragments arranged by size:
1572 1451 936 919 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3318 3348 1178
Size: 30 165 30 1915 30 4047
Fragments arranged by size:
4047 1915 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 1186
Size: 904 5313
BseYI C'CCAG_C
Cuts at: 2440 2697 2939 3025 3153 3410 5420 5916 2440
Size: 257 242 86 128 257 2010 496 2741
Fragments arranged by size:
2741 2010 496 257 257 242 128 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 3422 1163
Size: 573 489 1197 3958
Fragments arranged by size:
3958 1197 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2614 3667 4428 5456 1540
Size: 275 186 613 1053 761 1028 2301
Fragments arranged by size:
2301 1053 1028 761 613 275 186
BsiHKAI G_wGCw'C
Cuts at: 2544 2661 2970 4278 4363 5434 2544
Size: 117 309 1308 85 1071 3327
Fragments arranged by size:
3327 1308 1071 309 117 85
BsmI GAATG_Cn'
Cuts at: 3503 3728 3821 3503
Size: 225 93 5899
Fragments arranged by size:
5899 225 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2544 2661 2698 2781 2843 2923 2970
Size: 399 754 117 37 83 62 80 47
Cuts at: 2970 3221 3536 4278 4363 5434 1391
Size: 251 315 742 85 1071 2174
Fragments arranged by size:
2174 1071 754 742 399 315 251 117
85 83 80 62 47 37
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3303 3572 844
Size: 2459 269 3489
Fragments arranged by size:
3489 2459 269
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2448 2613 3172 4861 5048 1700
Size: 110 638 165 559 1689 187 2869
Fragments arranged by size:
2869 1689 638 559 187 165 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2502 2576 2758 3209 3445 794
Size: 1708 74 182 451 236 3566
Fragments arranged by size:
3566 1708 451 236 182 74
BstBI TT'CG_AA
Cuts at: 2007 5021 2007
Size: 3014 3203
BstEII G'GTnAC_C
Cuts at: 1184 2483 1184
Size: 1299 4918
BstXI CCAn_nnnn'nTGG
Cuts at: 2705 2705
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 3056 3547 3914 4299 4316 5768 5854 5866
Size: 1084 491 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1084 491 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2781 2904 2982 513
Size: 552 1716 123 78 3748
Fragments arranged by size:
3748 1716 552 123 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 2605 3203 3733 4527 4547 2404
Size: 201 598 530 794 20 4074
Fragments arranged by size:
4074 794 598 530 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DraIII CAC_nnn'GTG
Cuts at: 2653 2653
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2460 2707
Size: 54 1425 50 275 18 459 171 247
Cuts at: 2707 3664 4553 6182 8
Size: 957 889 1629 43
Fragments arranged by size:
1629 1425 957 889 459 275 247 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 2826 3025 4147 1353
Size: 1382 91 199 1122 3423
Fragments arranged by size:
3423 1382 1122 199 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 3128 4804 5178 5324 387
Size: 1064 138 549 990 1676 374 146 1280
Fragments arranged by size:
1676 1280 1064 990 549 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 4280 4877 5664 5932 1239
Size: 393 935 1713 597 787 268 1524
Fragments arranged by size:
1713 1524 935 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3233 3233
Size: 6217
EcoO109I rG'GnC_Cy
Cuts at: 2398 2530 3238 2398
Size: 132 708 5377
Fragments arranged by size:
5377 708 132
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3224 3224
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2455 5364 1678
Size: 777 2909 2531
Fragments arranged by size:
2909 2531 777
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2368 2400 3318 3350 4858
Size: 32 135 32 796 32 918 32 1508
Cuts at: 4858 4890 4932 4964 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1508 918 796 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2451 2451
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 2726 6093 2726
Size: 3367 2850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 2462 10
Size: 54 1425 343 459 171 3765
Fragments arranged by size:
3765 1425 459 343 171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2598 2909 2948 2987 4175 4534 4693
Size: 648 1432 311 39 39 1188 359 159
Cuts at: 4693 518
Size: 2042
Fragments arranged by size:
2042 1432 1188 648 359 311 159 39
39
MspA1I CmG'CkG
Cuts at: 1606 2239 2481 2493 2701 2805 3146 3182 3633
Size: 633 242 12 208 104 341 36 451
Cuts at: 3633 4310 5458 5703 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 451 341 245 242
208 104 36 12
NaeI GCC'GGC
Cuts at: 3174 3174
Size: 6217
NarI GG'CG_CC
Cuts at: 2452 2452
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2904 2982 513
Size: 552 1839 78 3748
Fragments arranged by size:
3748 1839 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 3172 3172
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NsiI A_TGCA'T
Cuts at: 2469 2469
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2471 2791 2899 2914 3575 5120 1132
Size: 97 1242 320 108 15 661 1545 2229
Fragments arranged by size:
2229 1545 1242 661 320 108 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 2787 5116 2787
Size: 2329 3888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3274 3410 1108
Size: 2166 136 3915
Fragments arranged by size:
3915 2166 136
PfoI T'CCnGG_A
Cuts at: 3121 3121
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PstI C_TGCA'G
Cuts at: 830 2376 2511 2810 3569 830
Size: 1546 135 299 759 3478
Fragments arranged by size:
3478 1546 759 299 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2239 2493 2805 3182 2239
Size: 254 312 377 5274
Fragments arranged by size:
5274 377 312 254
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2735 2826 2735
Size: 91 6126
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 2690 4465 6177 2690
Size: 1775 1712 2730
Fragments arranged by size:
2730 1775 1712
SfcI C'TryA_G
Cuts at: 826 1045 2372 2507 2806 3565 4700 5381 5572
Size: 219 1327 135 299 759 1135 681 191
Cuts at: 5572 826
Size: 1471
Fragments arranged by size:
1471 1327 1135 759 681 299 219 191
135
SfoI GGC'GCC
Cuts at: 2453 2453
Size: 6217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3393 4321 5222 5484 820
Size: 197 926 819 631 928 901 262 1553
Fragments arranged by size:
1553 928 926 901 819 631 262 197
SnaBI TAC'GTA
Cuts at: 493 3514 4008 493
Size: 3021 494 2702
Fragments arranged by size:
3021 2702 494
SpeI A'CTAG_T
Cuts at: 152 2095 152
Size: 1943 4274
SphI G_CATG'C
Cuts at: 1132 2471 3575 1132
Size: 1339 1104 3774
Fragments arranged by size:
3774 1339 1104
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2107 2191 2796 2885 3330 1517
Size: 590 84 605 89 445 4404
Fragments arranged by size:
4404 605 590 445 89 84
StyI C'CwwG_G
Cuts at: 513 1065 2580 2880 2904 2982 3656 513
Size: 552 1515 300 24 78 674 3074
Fragments arranged by size:
3074 1515 674 552 300 78 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2309 4244 4261 4414 4599 5044 325
Size: 1255 729 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 729 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2688 4463
Size: 274 80 33 51 1443 348 363 1775
Cuts at: 4463 6175 96
Size: 1712 138
Fragments arranged by size:
1775 1712 1443 363 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2666 2698 6177 6209 2666
Size: 32 3479 32 2674
Fragments arranged by size:
3479 2674 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3094 3328 3094
Size: 234 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI