Restriction map for
pFN21ASDA0195
(Circular) (Six-base) MAPSORT of: pFN21ASDA0195.seq Check: 7042 from: 1 to: 8797
pFN21ASDA0195 8797 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4914 4914
Size: 8797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6607 278
Size: 53 83 186 936 5071 2468
Fragments arranged by size:
5071 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4578 6140 1937
Size: 2641 1562 4594
Fragments arranged by size:
4594 2641 1562
Acc65I G'GTAC_C
Cuts at: 6118 6118
Size: 8797
AclI AA'CG_TT
Cuts at: 1563 6924 7297 1563
Size: 5361 373 3063
Fragments arranged by size:
5361 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2120 2399 2911 3310 3562 6860 8244
Size: 419 69 279 512 399 252 3298 1384
Cuts at: 8244 8512 1632
Size: 268 1917
Fragments arranged by size:
3298 1917 1384 512 419 399 279 268
252 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8600
AflIII A'CryG_T
Cuts at: 1203 7696 8673 1203
Size: 6493 977 1327
Fragments arranged by size:
6493 1327 977
AgeI A'CCGG_T
Cuts at: 7628 7628
Size: 8797
AhdI GACnn_n'nnGTC
Cuts at: 2543 7526 2543
Size: 4983 3814
AleI CACnn'nnGTG
Cuts at: 1166 5774 1166
Size: 4608 4189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5453 1184
Size: 4269 4528
AlwNI CAG_nnn'CTG
Cuts at: 2822 3314 4123 4416 4474 5568 5843 6052 6540
Size: 492 809 293 58 1094 275 209 488
Cuts at: 6540 8112 2822
Size: 1572 3507
Fragments arranged by size:
3507 1572 1094 809 492 488 293 275
209 58
ApaLI G'TGCA_C
Cuts at: 6854 8010 6854
Size: 1156 7641
ApoI r'AATT_y
Cuts at: 1493 6106 6332 6484 8593 1493
Size: 4613 226 152 2109 1697
Fragments arranged by size:
4613 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7351 160
Size: 872 6319 1606
Fragments arranged by size:
6319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8797
AvaI C'yCGr_G
Cuts at: 1870 1943 2700 2935 3191 3404 3512 4198 4755
Size: 73 757 235 256 213 108 686 557
Cuts at: 4755 4878 5616 6122 1870
Size: 123 738 506 4545
Fragments arranged by size:
4545 757 738 686 557 506 256 235
213 123 108 73
BamHI G'GATC_C
Cuts at: 3282 5086 6127 6494 3282
Size: 1804 1041 367 5585
Fragments arranged by size:
5585 1804 1041 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2790 4133 4336 6118 7573
Size: 325 217 627 1003 1343 203 1782 1455
Cuts at: 7573 618
Size: 1842
Fragments arranged by size:
1842 1782 1455 1343 1003 627 325 217
203
BanII G_rGCy'C
Cuts at: 1391 2732 3248 3345 3411 3519 3612 4424 4602
Size: 1341 516 97 66 108 93 812 178
Cuts at: 4602 4776 5677 5810 6116 1391
Size: 174 901 133 306 4072
Fragments arranged by size:
4072 1341 901 812 516 306 178 174
133 108 97 93 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4464 5516 5749 928
Size: 971 2565 1052 233 3976
Fragments arranged by size:
3976 2565 1052 971 233
BbvCI CC'TCA_GC
Cuts at: 2059 2090 4067 2059
Size: 31 1977 6789
Fragments arranged by size:
6789 1977 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 6988 7022 1903
Size: 34 2366 34 2651 34 3678
Fragments arranged by size:
3678 2651 2366 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3106 4895 6689 7899 2487
Size: 619 1789 1794 1210 3385
Fragments arranged by size:
3385 1794 1789 1210 619
BclI T'GATC_A
Cuts at: 1548 4077 1548
Size: 2529 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3431 3933 4722
Size: 107 122 71 631 605 1759 502 789
Cuts at: 4722 7408 136
Size: 2686 1525
Fragments arranged by size:
2686 1759 1525 789 631 605 502 122
107 71
BglII A'GATC_T
Cuts at: 4485 8792 4485
Size: 4307 4490
BlpI GC'TnA_GC
Cuts at: 3212 3689 5426 6214 3212
Size: 477 1737 788 5795
Fragments arranged by size:
5795 1737 788 477
Bme1580I G_kGCm'C
Cuts at: 1790 2880 2935 3903 4341 4929 4941 5940 6858
Size: 1090 55 968 438 588 12 999 918
Cuts at: 6858 8014 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1090 999 968 918 588 438
55 12
BmgBI CAC'GTC
Cuts at: 1318 2160 5914 5935 1318
Size: 842 3754 21 4180
Fragments arranged by size:
4180 3754 842 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2460 2530 3993
Size: 416 53 83 399 24 1043 70 1463
Cuts at: 3993 4504 6528 7486 442
Size: 511 2024 958 1753
Fragments arranged by size:
2024 1753 1463 1043 958 511 416 399
83 70 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 3157 3443 4816 2074
Size: 1083 286 1373 6055
Fragments arranged by size:
6055 1373 1083 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2675 2951 2957 3701 4438 4469 4655
Size: 893 543 276 6 744 737 31 186
Cuts at: 4655 4690 4871 5045 5477 5661 5948 7457 1239
Size: 35 181 174 432 184 287 1509 2579
Fragments arranged by size:
2579 1509 893 744 737 543 432 287
276 186 184 181 174 35 31 6
Bpu10I CC'TnA_GC
Cuts at: 2059 2090 4067 4475 2059
Size: 31 1977 408 6381
Fragments arranged by size:
6381 1977 408 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 5528 6922 7787 8085 2780
Size: 2748 1394 865 298 3492
Fragments arranged by size:
3492 2748 1394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2658 2756 4958 5111 5228 7460 882
Size: 617 1159 98 2202 153 117 2232 2219
Fragments arranged by size:
2232 2219 2202 1159 617 153 117 98
BsaAI yAC'GTr
Cuts at: 493 1204 6094 6588 493
Size: 711 4890 494 2702
Fragments arranged by size:
4890 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4985 8791 4985
Size: 3806 4991
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2985 3423 6604 6986
Size: 53 83 186 936 1452 438 3181 382
Cuts at: 6986 7621 275
Size: 635 1451
Fragments arranged by size:
3181 1452 1451 936 635 438 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7230 7628 7902 8049 8460 7230
Size: 398 274 147 411 7567
Fragments arranged by size:
7567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2444 2474 3746
Size: 30 165 30 789 30 222 30 1272
Cuts at: 3746 3776 4709 4739 5627 5657 1178
Size: 30 933 30 888 30 4318
Fragments arranged by size:
4318 1272 933 888 789 222 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3133 4766 4769 4802 4805 5246 5632
Size: 1768 179 1633 3 33 3 441 386
Cuts at: 5632 1186
Size: 4351
Fragments arranged by size:
4351 1768 1633 441 386 179 33 3
3
BseYI C'CCAG_C
Cuts at: 2263 2733 3083 3302 3611 3621 4027 4376 4712
Size: 470 350 219 309 10 406 349 336
Cuts at: 4712 4841 5440 5514 5657 5854 6070 8000 8496
Size: 129 599 74 143 197 216 1930 496
Cuts at: 8496 2263
Size: 2564
Fragments arranged by size:
2564 1930 599 496 470 406 350 349
336 309 219 216 197 143 129 74
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2870 3218 3440 3737 4144 4939 5732
Size: 573 1134 348 222 297 407 795 793
Cuts at: 5732 1163
Size: 4228
Fragments arranged by size:
4228 1134 795 793 573 407 348 297
222
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5733 6247 7008 8036 1540
Size: 275 186 3732 514 761 1028 2301
Fragments arranged by size:
3732 2301 1028 761 514 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 2646 3192 4424 4602 6858 6943 8014 2358
Size: 288 546 1232 178 2256 85 1071 3141
Fragments arranged by size:
3141 2256 1232 1071 546 288 178 85
BsmI GAATG_Cn'
Cuts at: 2320 6308 6401 2320
Size: 3988 93 4716
Fragments arranged by size:
4716 3988 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2526 3414 2526
Size: 888 7909
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2358 2646 2732 2880 2935 3192 3248
Size: 399 568 288 86 148 55 257 56
Cuts at: 3248 3345 3411 3519 3612 3903 4341 4424 4602
Size: 97 66 108 93 291 438 83 178
Cuts at: 4602 4776 4929 4941 5677 5810 5940 6116 6858
Size: 174 153 12 736 133 130 176 742
Cuts at: 6858 6943 8014 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 568 438 399 291
288 257 178 176 174 153 148 133
130 108 97 93 86 85 83 66
56 55 12
BspEI T'CCGG_A
Cuts at: 8460 8460
Size: 8797
BspHI T'CATG_A
Cuts at: 6686 8416 6686
Size: 1730 7067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 4914 5021 5046 5892 6152 844
Size: 1715 2355 107 25 846 260 3489
Fragments arranged by size:
3489 2355 1715 846 260 107 25
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 2398 2839 3390 4479 6684 1394
Size: 429 320 255 441 551 1089 2205 3507
Fragments arranged by size:
3507 2205 1089 551 441 429 320 255
BsrDI GCAATG_nn'
Cuts at: 65 1248 3855 5076 7292 7466 65
Size: 1183 2607 1221 2216 174 1396
Fragments arranged by size:
2607 2216 1396 1221 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2373 2742 2891 7441 7628 1700
Size: 110 563 369 149 4550 187 2869
Fragments arranged by size:
4550 2869 563 369 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8797
BssSI C'ACGA_G
Cuts at: 6857 7869 6857
Size: 1012 7785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8797
BstBI TT'CG_AA
Cuts at: 2007 7601 2007
Size: 5594 3203
BstEII G'GTnAC_C
Cuts at: 1184 5008 5986 1184
Size: 3824 978 3995
Fragments arranged by size:
3995 3824 978
BstXI CCAn_nnnn'nTGG
Cuts at: 2981 3021 4849 2981
Size: 40 1828 6929
Fragments arranged by size:
6929 1828 40
BstYI r'GATC_y
Cuts at: 1972 2454 3282 4202 4485 4695 5086 6127 6494
Size: 482 828 920 283 210 391 1041 367
Cuts at: 6494 6879 6896 8348 8434 8446 8792 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1041 920 828 482 391 385
367 346 283 210 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2438 3182 2438
Size: 744 8053
BtgI C'CryG_G
Cuts at: 513 1065 2022 2296 2880 4129 513
Size: 552 957 274 584 1249 5181
Fragments arranged by size:
5181 1249 957 584 552 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3186 4008 5765 487
Size: 683 56 1960 822 1757 3519
Fragments arranged by size:
3519 1960 1757 822 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2820 5321 5591 6313 7107 7127 2124
Size: 696 2501 270 722 794 20 3794
Fragments arranged by size:
3794 2501 794 722 696 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8762
DraI TTT'AAA
Cuts at: 6101 6101
Size: 8797
DraIII CAC_nnn'GTG
Cuts at: 4365 4894 4365
Size: 529 8268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5912 7804 809
Size: 507 4596 1892 1802
Fragments arranged by size:
4596 1892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2222 2247 2599
Size: 54 1425 50 275 18 392 25 352
Cuts at: 2599 2889 3293 3541 4342 4480 5730 6244 7133
Size: 290 404 248 801 138 1250 514 889
Cuts at: 7133 8762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1250 889 801 514 404 392
352 290 275 248 138 54 50 43
25 18
EagI C'GGCC_G
Cuts at: 1537 1812 5730 6244 1537
Size: 275 3918 514 4090
Fragments arranged by size:
4090 3918 514 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3600 4091 5060 5309 6727 1353
Size: 1322 925 491 969 249 1418 3423
Fragments arranged by size:
3423 1418 1322 969 925 491 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7384 7758 7904 387
Size: 1064 138 5795 374 146 1280
Fragments arranged by size:
5795 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4422 4600 4422
Size: 178 8619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2120 2132 2399 2675 2911 2951
Size: 393 419 69 12 267 276 236 40
Cuts at: 2951 2957 3310 3562 3701 4438 4469 4655 4690
Size: 6 353 252 139 737 31 186 35
Cuts at: 4690 4871 5045 5477 5661 5948 6860 7457 8244
Size: 181 174 432 184 287 912 597 787
Cuts at: 8244 8512 1239
Size: 268 1524
Fragments arranged by size:
1524 912 787 737 597 432 419 393
353 287 276 268 267 252 236 186
184 181 174 139 69 40 35 31
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3698 4745 4784 3698
Size: 1047 39 7711
Fragments arranged by size:
7711 1047 39
EcoO109I rG'GnC_Cy
Cuts at: 2086 2229 3364 3907 4471 2086
Size: 143 1135 543 564 6412
Fragments arranged by size:
6412 1135 564 543 143
EcoRI G'AATT_C
Cuts at: 6106 6484 6106
Size: 378 8419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5827 5827
Size: 8797
FspI TGC'GCA
Cuts at: 7303 7303
Size: 8797
HaeII r_GCGC'y
Cuts at: 1678 3826 3882 3986 7944 1678
Size: 2148 56 104 3958 2531
Fragments arranged by size:
3958 2531 2148 104 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2529 2561 3365 3397 4170
Size: 32 135 32 957 32 804 32 773
Cuts at: 4170 4202 7438 7470 7512 7544 1373
Size: 32 3236 32 42 32 2626
Fragments arranged by size:
3236 2626 957 804 773 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 4558 6141 6387 669
Size: 988 281 610 2010 1583 246 3079
Fragments arranged by size:
3079 2010 1583 988 610 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8797
HpaI GTT'AAC
Cuts at: 6387 6387
Size: 8797
KpnI G_GTAC'C
Cuts at: 6122 6122
Size: 8797
MfeI C'AATT_G
Cuts at: 6396 6396
Size: 8797
MluI A'CGCG_T
Cuts at: 8673 8673
Size: 8797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3591 3765 3864 4884 7911 8095 1305
Size: 2286 174 99 1020 3027 184 2007
Fragments arranged by size:
3027 2286 2007 1020 184 174 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2601 3295 10
Size: 54 1425 343 769 694 5512
Fragments arranged by size:
5512 1425 769 694 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4496 5144 5471 5774 6755 7114 7273
Size: 648 3330 648 327 303 981 359 159
Cuts at: 7273 518
Size: 2042
Fragments arranged by size:
3330 2042 981 648 648 359 327 303
159
MspA1I CmG'CkG
Cuts at: 1606 2110 2205 3330 3751 4139 4416 5049 5182
Size: 504 95 1125 421 388 277 633 133
Cuts at: 5182 5323 5365 5890 6213 6890 8038 8283 1606
Size: 141 42 525 323 677 1148 245 2120
Fragments arranged by size:
2120 1148 1125 677 633 525 504 421
388 323 277 245 141 133 95 42
NaeI GCC'GGC
Cuts at: 2893 2893
Size: 8797
NcoI C'CATG_G
Cuts at: 513 1065 2022 4129 513
Size: 552 957 2107 5181
Fragments arranged by size:
5181 2107 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8797
NgoMIV G'CCGG_C
Cuts at: 2891 2891
Size: 8797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8797
NotI GC'GGCC_GC
Cuts at: 6244 6244
Size: 8797
NspI r_CATG'y
Cuts at: 1132 1229 2494 4468 4684 5276 6155 7700 1132
Size: 97 1265 1974 216 592 879 1545 2229
Fragments arranged by size:
2229 1974 1545 1265 879 592 216 97
PasI CC'CwG_GG
Cuts at: 1348 2065 3775 3903 1348
Size: 717 1710 128 6242
Fragments arranged by size:
6242 1710 717 128
PciI A'CATG_T
Cuts at: 7696 7696
Size: 8797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5823 1108
Size: 4715 4082
PfoI T'CCnGG_A
Cuts at: 2652 4848 2652
Size: 2196 6601
PmeI GTTT'AAAC
Cuts at: 6101 6101
Size: 8797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3237 6866 6898 8379 8411 3205
Size: 32 3629 32 1481 32 3591
Fragments arranged by size:
3629 3591 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3364 3364
Size: 8797
PshAI GACnn'nnGTC
Cuts at: 1613 5710 1613
Size: 4097 4700
PsiI TTA'TAA
Cuts at: 6367 6367
Size: 8797
PstI C_TGCA'G
Cuts at: 830 4911 5181 5316 5598 5889 6057 6149 830
Size: 4081 270 135 282 291 168 92 3478
Fragments arranged by size:
4081 3478 291 282 270 168 135 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8797
PvuII CAG'CTG
Cuts at: 2110 3751 4416 5049 5182 5323 5365 5890 2110
Size: 1641 665 633 133 141 42 525 5017
Fragments arranged by size:
5017 1641 665 633 525 141 133 42
SacI G_AGCT'C
Cuts at: 4424 4602 4424
Size: 178 8619
SalI G'TCGA_C
Cuts at: 1936 6139 1936
Size: 4203 4594
SapI GCTCTTCn'nnn_
Cuts at: 3600 3600
Size: 8797
SbfI CC_TGCA'GG
Cuts at: 6149 6149
Size: 8797
ScaI AGT'ACT
Cuts at: 7045 8757 7045
Size: 1712 7085
SexAI A'CCwGG_T
Cuts at: 2541 2541
Size: 8797
SfcI C'TryA_G
Cuts at: 826 1045 2527 4907 5177 5312 5594 5885 6053
Size: 219 1482 2380 270 135 282 291 168
Cuts at: 6053 6145 7280 7961 8152 826
Size: 92 1135 681 191 1471
Fragments arranged by size:
2380 1482 1471 1135 681 291 282 270
219 191 168 135 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8797
SmaI CCC'GGG
Cuts at: 1872 2702 2937 4880 5618 6124 1872
Size: 830 235 1943 738 506 4545
Fragments arranged by size:
4545 1943 830 738 506 235
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 5543 6901 7802 8064 820
Size: 197 926 816 2784 1358 901 262 1553
Fragments arranged by size:
2784 1553 1358 926 901 816 262 197
SnaBI TAC'GTA
Cuts at: 493 6094 6588 493
Size: 5601 494 2702
Fragments arranged by size:
5601 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8797
SphI G_CATG'C
Cuts at: 1132 4468 5276 6155 1132
Size: 3336 808 879 3774
Fragments arranged by size:
3774 3336 879 808
SspI AAT'ATT
Cuts at: 5 52 6721 5
Size: 47 6669 2081
Fragments arranged by size:
6669 2081 47
StuI AGG'CCT
Cuts at: 1517 2596 3077 4157 5156 1517
Size: 1079 481 1080 999 5158
Fragments arranged by size:
5158 1080 1079 999 481
StyI C'CwwG_G
Cuts at: 513 1065 2022 3951 4129 6236 513
Size: 552 957 1929 178 2107 3074
Fragments arranged by size:
3074 2107 1929 957 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3097 3570 4684 5786 6824 6841 6994
Size: 1255 1517 473 1114 1102 1038 17 153
Cuts at: 6994 7179 7624 325
Size: 185 445 1498
Fragments arranged by size:
1517 1498 1255 1114 1102 1038 473 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2955 3316 7043
Size: 274 80 33 51 1443 978 361 3727
Cuts at: 7043 8755 96
Size: 1712 138
Fragments arranged by size:
3727 1712 1443 978 361 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2444 2476 3650 3682 3746 3778 4709 4741 8757
Size: 32 1174 32 64 32 931 32 4016
Cuts at: 8757 8789 2444
Size: 32 2452
Fragments arranged by size:
4016 2452 1174 931 64 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8797
XbaI T'CTAG_A
Cuts at: 6133 6133
Size: 8797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3091 3536 5143 5159 5470 5918 3091
Size: 445 1607 16 311 448 5970
Fragments arranged by size:
5970 1607 448 445 311 16
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8797
XmaI C'CCGG_G
Cuts at: 1870 2700 2935 4878 5616 6122 1870
Size: 830 235 1943 738 506 4545
Fragments arranged by size:
4545 1943 830 738 506 235
XmnI GAAnn'nnTTC
Cuts at: 6926 6926
Size: 8797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6605 276
Size: 53 83 186 936 5071 2468
Fragments arranged by size:
5071 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI