Restriction map for
pFN21ASDA0181
(Circular) (Six-base) MAPSORT of: pFN21ASDA0181.seq Check: 3907 from: 1 to: 10918
pFN21ASDA0181 10918 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3686 3724 3686
Size: 38 10880
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8728 278
Size: 53 83 186 936 7192 2468
Fragments arranged by size:
7192 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7210 8261 1937
Size: 5273 1051 4594
Fragments arranged by size:
5273 4594 1051
Acc65I G'GTAC_C
Cuts at: 7786 8239 7786
Size: 453 10465
AclI AA'CG_TT
Cuts at: 1563 6732 9045 9418 1563
Size: 5169 2313 373 3063
Fragments arranged by size:
5169 3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4955 5442 5709 6405 6831 7599 8103 8133
Size: 3323 487 267 696 426 768 504 30
Cuts at: 8133 8981 10365 10633 1632
Size: 848 1384 268 1917
Fragments arranged by size:
3323 1917 1384 848 768 696 504 487
426 268 267 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10721
AflIII A'CryG_T
Cuts at: 1203 5479 9817 10794 1203
Size: 4276 4338 977 1327
Fragments arranged by size:
4338 4276 1327 977
AgeI A'CCGG_T
Cuts at: 9749 9749
Size: 10918
AhdI GACnn_n'nnGTC
Cuts at: 7217 7419 9647 7217
Size: 202 2228 8488
Fragments arranged by size:
8488 2228 202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5292 1184
Size: 4108 6810
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5198 5230 5198
Size: 32 10886
AlwNI CAG_nnn'CTG
Cuts at: 4321 4384 6356 6631 8661 10233 4321
Size: 63 1972 275 2030 1572 5006
Fragments arranged by size:
5006 2030 1972 1572 275 63
ApaI G_GGCC'C
Cuts at: 3255 3872 3982 4038 4088 4401 5201 7709 3255
Size: 617 110 56 50 313 800 2508 6464
Fragments arranged by size:
6464 2508 800 617 313 110 56 50
ApaLI G'TGCA_C
Cuts at: 8975 10131 8975
Size: 1156 9762
ApoI r'AATT_y
Cuts at: 1493 2491 2925 3649 3780 4916 5581 5815 5953
Size: 998 434 724 131 1136 665 234 138
Cuts at: 5953 6064 6220 6307 6454 7142 7497 7927 8227
Size: 111 156 87 147 688 355 430 300
Cuts at: 8227 8453 8605 10714 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 998 724 688 665 434
430 355 300 234 226 156 152 147
138 131 111 87
AseI AT'TA_AT
Cuts at: 160 1032 5790 9472 160
Size: 872 4758 3682 1606
Fragments arranged by size:
4758 3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10918
AvaI C'yCGr_G
Cuts at: 1870 1943 2325 2578 4018 4546 7708 8243 1870
Size: 73 382 253 1440 528 3162 535 4545
Fragments arranged by size:
4545 3162 1440 535 528 382 253 73
AvrII C'CTAG_G
Cuts at: 3544 5807 3544
Size: 2263 8655
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7770 7803 7770
Size: 33 10885
BamHI G'GATC_C
Cuts at: 2349 2674 3335 5146 6418 7886 8248 8615 2349
Size: 325 661 1811 1272 1468 362 367 4652
Fragments arranged by size:
4652 1811 1468 1272 661 367 362 325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2608 2956 3619 4099 5005
Size: 325 217 627 821 348 663 480 906
Cuts at: 5005 7786 8239 9694 618
Size: 2781 453 1455 1842
Fragments arranged by size:
2781 1842 1455 906 821 663 627 480
453 348 325 217
BanII G_rGCy'C
Cuts at: 1391 2292 2694 2998 3255 3661 3872 3982 4038
Size: 901 402 304 257 406 211 110 56
Cuts at: 4038 4088 4401 4603 4676 5201 5375 6523 7614
Size: 50 313 202 73 525 174 1148 1091
Cuts at: 7614 7709 7800 8031 8237 1391
Size: 95 91 231 206 4072
Fragments arranged by size:
4072 1148 1091 901 525 406 402 313
304 257 231 211 206 202 174 110
95 91 73 56 50
BbeI G_GCGC'C
Cuts at: 4103 4103
Size: 10918
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2094 6627 7263 928
Size: 971 159 36 4533 636 4583
Fragments arranged by size:
4583 4533 971 636 159 36
BbvCI CC'TCA_GC
Cuts at: 3663 3839 5052 7354 7720 3663
Size: 176 1213 2302 366 6861
Fragments arranged by size:
6861 2302 1213 366 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3589 3623 6471 6505 9109 9143 1903
Size: 34 1652 34 2848 34 2604 34 3678
Fragments arranged by size:
3678 2848 2604 1652 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8810 10020 8810
Size: 1210 9708
BclI T'GATC_A
Cuts at: 1548 4852 5833 1548
Size: 3304 981 6633
Fragments arranged by size:
6633 3304 981
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 4396 7704
Size: 107 122 71 631 605 2457 267 3308
Cuts at: 7704 9529 136
Size: 1825 1525
Fragments arranged by size:
3308 2457 1825 1525 631 605 267 122
107 71
BglII A'GATC_T
Cuts at: 4807 6556 10913 4807
Size: 1749 4357 4812
Fragments arranged by size:
4812 4357 1749
BlpI GC'TnA_GC
Cuts at: 8335 8335
Size: 10918
Bme1580I G_kGCm'C
Cuts at: 1790 2241 3032 3255 3872 3912 3982 4038 4088
Size: 451 791 223 617 40 70 56 50
Cuts at: 4088 4401 5201 7709 7715 8979 10135 1790
Size: 313 800 2508 6 1264 1156 2573
Fragments arranged by size:
2573 2508 1264 1156 800 791 617 451
313 223 70 56 50 40 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2929 4640 5070
Size: 416 53 83 399 24 1512 1711 430
Cuts at: 5070 6429 7445 8649 9607 442
Size: 1359 1016 1204 958 1753
Fragments arranged by size:
1753 1711 1512 1359 1204 1016 958 430
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 7338 1056
Size: 6282 4636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5428 7819 5428
Size: 2391 8527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2952 2994 3012 3077 4890 5997 6537 6558
Size: 1713 42 18 65 1813 1107 540 21
Cuts at: 6558 6735 7162 7260 8009 8140 9578 1239
Size: 177 427 98 749 131 1438 2579
Fragments arranged by size:
2579 1813 1713 1438 1107 749 540 427
177 131 98 65 42 21 18
Bpu10I CC'TnA_GC
Cuts at: 3109 3663 3839 5052 7223 7354 7720 3109
Size: 554 176 1213 2171 131 366 6307
Fragments arranged by size:
6307 2171 1213 554 366 176 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3813 5535 7559 9043 9908 10206 3813
Size: 1722 2024 1484 865 298 4525
Fragments arranged by size:
4525 2024 1722 1484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9581 882
Size: 617 8082 2219
Fragments arranged by size:
8082 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 8215 8709 493
Size: 711 7011 494 2702
Fragments arranged by size:
7011 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 10912 10912
Size: 10918
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3125 4100 8725 9107
Size: 53 83 186 936 1592 975 4625 382
Cuts at: 9107 9742 275
Size: 635 1451
Fragments arranged by size:
4625 1592 1451 975 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 9351 9749 10023 10170 10581 9351
Size: 398 274 147 411 9688
Fragments arranged by size:
9688 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3075 3105 5198 5228 7258
Size: 30 165 30 1672 30 2093 30 2030
Cuts at: 7258 7288 7588 7618 1178
Size: 30 300 30 4478
Fragments arranged by size:
4478 2093 2030 1672 300 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2634 2672 2997 3216 3301 3310 3708 4974
Size: 1448 38 325 219 85 9 398 1266
Cuts at: 4974 6150 7889 1186
Size: 1176 1739 4215
Fragments arranged by size:
4215 1739 1448 1266 1176 398 325 219
85 38 9
BseYI C'CCAG_C
Cuts at: 2782 2796 3767 3861 3981 4077 4326 4400 4416
Size: 14 971 94 120 96 249 74 16
Cuts at: 4416 4831 5565 5591 5711 7666 7730 7907 10121
Size: 415 734 26 120 1955 64 177 2214
Cuts at: 10121 10617 2782
Size: 496 3083
Fragments arranged by size:
3083 2214 1955 971 734 496 415 249
177 120 120 96 94 74 64 26
16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4310 4541 7322 7584 1163
Size: 573 2574 231 2781 262 4497
Fragments arranged by size:
4497 2781 2574 573 262 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8368 9129 10157 1540
Size: 275 186 6367 761 1028 2301
Fragments arranged by size:
6367 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4603 6523 7750 7800 8979 9064 10135 4603
Size: 1920 1227 50 1179 85 1071 5386
Fragments arranged by size:
5386 1920 1227 1179 1071 85 50
BsmI GAATG_Cn'
Cuts at: 3558 4368 4488 5229 5316 8429 8522 3558
Size: 810 120 741 87 3113 93 5954
Fragments arranged by size:
5954 3113 810 741 120 93 87
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2292 2694 2998 3032 3255 3661
Size: 399 451 51 402 304 34 223 406
Cuts at: 3661 3872 3912 3982 4038 4088 4401 4603 4676
Size: 211 40 70 56 50 313 202 73
Cuts at: 4676 5201 5375 6523 7614 7709 7715 7750 7800
Size: 525 174 1148 1091 95 6 35 50
Cuts at: 7800 8031 8237 8979 9064 10135 1391
Size: 231 206 742 85 1071 2174
Fragments arranged by size:
2174 1148 1091 1071 742 525 451 406
402 399 313 304 231 223 211 206
202 174 95 85 73 70 56 51
50 50 40 35 34 6
BspEI T'CCGG_A
Cuts at: 10581 10581
Size: 10918
BspHI T'CATG_A
Cuts at: 5133 8807 10537 5133
Size: 3674 1730 5514
Fragments arranged by size:
5514 3674 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3686 3724 4123 5284 6003 8152 8273 844
Size: 2842 38 399 1161 719 2149 121 3489
Fragments arranged by size:
3489 2842 2149 1161 719 399 121 38
BsrBI CCG'CTC
Cuts at: 1394 1823 2341 8805 1394
Size: 429 518 6464 3507
Fragments arranged by size:
6464 3507 518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 9413 9587 65
Size: 1183 1428 6737 174 1396
Fragments arranged by size:
6737 1428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4926 7440 9562 9749 1700
Size: 110 3116 2514 2122 187 2869
Fragments arranged by size:
3116 2869 2514 2122 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10918
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10918
BssSI C'ACGA_G
Cuts at: 7230 8978 9990 7230
Size: 1748 1012 8158
Fragments arranged by size:
8158 1748 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3907 4138 4528 6356 794
Size: 3113 231 390 1828 5356
Fragments arranged by size:
5356 3113 1828 390 231
BstBI TT'CG_AA
Cuts at: 2007 9722 2007
Size: 7715 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10918
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3775 4031 4424 6163 7642 2029
Size: 1746 256 393 1739 1479 5305
Fragments arranged by size:
5305 1746 1739 1479 393 256
BstYI r'GATC_y
Cuts at: 1972 2349 2674 2723 3335 4807 5146 6418 6556
Size: 377 325 49 612 1472 339 1272 138
Cuts at: 6556 6628 7886 8248 8615 9000 9017 10469 10555
Size: 72 1258 362 367 385 17 1452 86
Cuts at: 10555 10567 10913 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1472 1452 1272 1258 612 385 377
367 362 346 339 325 138 86 72
49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2719 4125 6288 7341 2719
Size: 1406 2163 1053 6296
Fragments arranged by size:
6296 2163 1406 1053
BtgI C'CryG_G
Cuts at: 513 1065 2022 2478 3114 3742 4567 513
Size: 552 957 456 636 628 825 6864
Fragments arranged by size:
6864 957 825 636 628 552 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5517 487
Size: 683 56 4291 5888
Fragments arranged by size:
5888 4291 683 56
BtsI GCAGTG_nn'
Cuts at: 5251 5440 6620 7713 7958 8029 8434 9228 9248
Size: 189 1180 1093 245 71 405 794 20
Cuts at: 9248 5251
Size: 6921
Fragments arranged by size:
6921 1180 1093 794 405 245 189 71
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2149 2151 2184 2186 7816 7851 529
Size: 35 1585 2 33 2 5630 35 3596
Fragments arranged by size:
5630 3596 1585 35 35 33 2 2
DraI TTT'AAA
Cuts at: 5820 8222 5820
Size: 2402 8516
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9925 809
Size: 507 8609 1802
Fragments arranged by size:
8609 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4388 4928 5602
Size: 54 1425 50 275 18 2558 540 674
Cuts at: 5602 6165 7492 8365 9254 10883 8
Size: 563 1327 873 889 1629 43
Fragments arranged by size:
2558 1629 1425 1327 889 873 674 563
540 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8365 1537
Size: 275 6553 4090
Fragments arranged by size:
6553 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3230 4839 5780 6438 6781 7377 7384 8079
Size: 1877 1609 941 658 343 596 7 695
Cuts at: 8079 8848 1353
Size: 769 3423
Fragments arranged by size:
3423 1877 1609 941 769 695 658 596
343 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5417 7795 9505 9879 10025 387
Size: 1064 138 3828 2378 1710 374 146 1280
Fragments arranged by size:
3828 2378 1710 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4601 6521 7798 4601
Size: 1920 1277 7721
Fragments arranged by size:
7721 1920 1277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2952 2994 3012 3077 4890 4955 5442
Size: 393 1320 42 18 65 1813 65 487
Cuts at: 5442 5709 5997 6405 6537 6558 6735 6831 7162
Size: 267 288 408 132 21 177 96 331
Cuts at: 7162 7260 7599 8009 8103 8133 8140 8981 9578
Size: 98 339 410 94 30 7 841 597
Cuts at: 9578 10365 10633 1239
Size: 787 268 1524
Fragments arranged by size:
1813 1524 1320 841 787 597 487 410
408 393 339 331 288 268 267 177
132 98 96 94 65 65 42 30
21 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 7871 7871
Size: 10918
EcoO109I rG'GnC_Cy
Cuts at: 2615 3251 3252 3262 3868 3978 4035 4084 4121
Size: 636 1 10 606 110 57 49 37
Cuts at: 4121 4334 4381 4397 4901 5197 5198 5273 6592
Size: 213 47 16 504 296 1 75 1319
Cuts at: 6592 7428 7727 2615
Size: 836 299 5806
Fragments arranged by size:
5806 1319 836 636 606 504 299 296
213 110 75 57 49 47 37 16
10 1 1
EcoRI G'AATT_C
Cuts at: 2925 3649 3780 5953 6220 6307 8227 8605 2925
Size: 724 131 2173 267 87 1920 378 5238
Fragments arranged by size:
5238 2173 1920 724 378 267 131 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 6292 3082
Size: 3210 7708
FspI TGC'GCA
Cuts at: 9424 9424
Size: 10918
HaeII r_GCGC'y
Cuts at: 1678 4103 10065 1678
Size: 2425 5962 2531
Fragments arranged by size:
5962 2531 2425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2341 2373 3520
Size: 32 135 32 521 32 216 32 1147
Cuts at: 3520 3552 4012 4044 4108 4140 4969 5001 5138
Size: 32 460 32 64 32 829 32 137
Cuts at: 5138 5170 6938 6970 7203 7235 7405 7437 7616
Size: 32 1768 32 233 32 170 32 179
Cuts at: 7616 7648 9559 9591 9633 9665 1373
Size: 32 1911 32 42 32 2626
Fragments arranged by size:
2626 1911 1768 1147 829 521 460 233
216 179 170 137 135 64 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 4687 6500 6900 7211 7389
Size: 988 281 1423 1326 1813 400 311 178
Cuts at: 7389 7506 8262 8508 669
Size: 117 756 246 3079
Fragments arranged by size:
3079 1813 1423 1326 988 756 400 311
281 246 178 117
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10918
HpaI GTT'AAC
Cuts at: 3361 8508 3361
Size: 5147 5771
KasI G'GCGC_C
Cuts at: 4099 4099
Size: 10918
KpnI G_GTAC'C
Cuts at: 7790 8243 7790
Size: 453 10465
MfeI C'AATT_G
Cuts at: 8517 8517
Size: 10918
MluI A'CGCG_T
Cuts at: 10794 10794
Size: 10918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3428 4168 5876 10032 10216 1305
Size: 2123 740 1708 4156 184 2007
Fragments arranged by size:
4156 2123 2007 1708 740 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6167 7494 10
Size: 54 1425 343 4335 1327 3434
Fragments arranged by size:
4335 3434 1425 1327 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3494 3696 6678 8876 9235 9394
Size: 648 1401 927 202 2982 2198 359 159
Cuts at: 9394 518
Size: 2042
Fragments arranged by size:
2982 2198 2042 1401 927 648 359 202
159
MspA1I CmG'CkG
Cuts at: 1606 2336 2800 2983 3667 3883 4835 6959 8334
Size: 730 464 183 684 216 952 2124 1375
Cuts at: 8334 9011 10159 10404 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2124 2120 1375 1148 952 730 684 677
464 245 216 183
NarI GG'CG_CC
Cuts at: 4100 4100
Size: 10918
NcoI C'CATG_G
Cuts at: 513 1065 2022 2478 4567 513
Size: 552 957 456 2089 6864
Fragments arranged by size:
6864 2089 957 552 456
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10918
NheI G'CTAG_C
Cuts at: 1052 7334 1052
Size: 6282 4636
NotI GC'GGCC_GC
Cuts at: 8365 8365
Size: 10918
NspI r_CATG'y
Cuts at: 1132 1229 3695 3850 3895 4276 5003 5473 5483
Size: 97 2466 155 45 381 727 470 10
Cuts at: 5483 7518 8276 9821 1132
Size: 2035 758 1545 2229
Fragments arranged by size:
2466 2229 2035 1545 758 727 470 381
155 97 45 10
PasI CC'CwG_GG
Cuts at: 1348 3016 3415 3644 3916 6053 6545 1348
Size: 1668 399 229 272 2137 492 5721
Fragments arranged by size:
5721 2137 1668 492 399 272 229
PciI A'CATG_T
Cuts at: 5479 9817 5479
Size: 4338 6580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4006 4117 5525 6053 1108
Size: 2898 111 1408 528 5973
Fragments arranged by size:
5973 2898 1408 528 111
PfoI T'CCnGG_A
Cuts at: 2533 2533
Size: 10918
PmeI GTTT'AAAC
Cuts at: 8222 8222
Size: 10918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 8987 9019 10500 10532 5198
Size: 32 3757 32 1481 32 5584
Fragments arranged by size:
5584 3757 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3262 4381 5273 6592 7428 7727 3262
Size: 1119 892 1319 836 299 6453
Fragments arranged by size:
6453 1319 1119 892 836 299
PshAI GACnn'nnGTC
Cuts at: 1613 4306 1613
Size: 2693 8225
PsiI TTA'TAA
Cuts at: 8488 8488
Size: 10918
PspOMI G'GGCC_C
Cuts at: 3251 3868 3978 4034 4084 4397 5197 7705 3251
Size: 617 110 56 50 313 800 2508 6464
Fragments arranged by size:
6464 2508 800 617 313 110 56 50
PstI C_TGCA'G
Cuts at: 830 2634 2805 4714 6244 6627 7063 7965 8270
Size: 1804 171 1909 1530 383 436 902 305
Cuts at: 8270 830
Size: 3478
Fragments arranged by size:
3478 1909 1804 1530 902 436 383 305
171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10918
PvuII CAG'CTG
Cuts at: 2800 2983 3667 3883 4835 6959 2800
Size: 183 684 216 952 2124 6759
Fragments arranged by size:
6759 2124 952 684 216 183
SacI G_AGCT'C
Cuts at: 4603 6523 7800 4603
Size: 1920 1277 7721
Fragments arranged by size:
7721 1920 1277
SalI G'TCGA_C
Cuts at: 1936 7209 8260 1936
Size: 5273 1051 4594
Fragments arranged by size:
5273 4594 1051
SanDI GG'GwC_CC
Cuts at: 3262 3262
Size: 10918
SapI GCTCTTCn'nnn_
Cuts at: 6781 8079 6781
Size: 1298 9620
SbfI CC_TGCA'GG
Cuts at: 8270 8270
Size: 10918
ScaI AGT'ACT
Cuts at: 5487 9166 10878 5487
Size: 3679 1712 5527
Fragments arranged by size:
5527 3679 1712
SexAI A'CCwGG_T
Cuts at: 4933 4933
Size: 10918
SfcI C'TryA_G
Cuts at: 826 1045 2630 2801 4440 4710 6240 6623 7059
Size: 219 1585 171 1639 270 1530 383 436
Cuts at: 7059 7961 8266 9401 10082 10273 826
Size: 902 305 1135 681 191 1471
Fragments arranged by size:
1639 1585 1530 1471 1135 902 681 436
383 305 270 219 191 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 4396 4396
Size: 10918
SfoI GGC'GCC
Cuts at: 4101 4101
Size: 10918
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10918
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10918
SmaI CCC'GGG
Cuts at: 1872 2327 2580 7710 8245 1872
Size: 455 253 5130 535 4545
Fragments arranged by size:
5130 4545 535 455 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3828 5550 7538 9022 9923 10185
Size: 197 926 1885 1722 1988 1484 901 262
Cuts at: 10185 820
Size: 1553
Fragments arranged by size:
1988 1885 1722 1553 1484 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 8215 8709 493
Size: 7722 494 2702
Fragments arranged by size:
7722 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10918
SphI G_CATG'C
Cuts at: 1132 3695 3895 8276 1132
Size: 2563 200 4381 3774
Fragments arranged by size:
4381 3774 2563 200
SrfI GCCC'GGGC
Cuts at: 7710 7710
Size: 10918
SspI AAT'ATT
Cuts at: 5 52 2226 5794 8842 5
Size: 47 2174 3568 3048 2081
Fragments arranged by size:
3568 3048 2174 2081 47
StuI AGG'CCT
Cuts at: 1517 3543 5844 7967 1517
Size: 2026 2301 2123 4468
Fragments arranged by size:
4468 2301 2123 2026
StyI C'CwwG_G
Cuts at: 513 1065 2022 2478 3046 3386 3544 4567 5192
Size: 552 957 456 568 340 158 1023 625
Cuts at: 5192 5334 5807 7509 7585 8357 513
Size: 142 473 1702 76 772 3074
Fragments arranged by size:
3074 1702 1023 957 772 625 568 552
473 456 340 158 142 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2760 4075 5710 7687 8945 8962 9115
Size: 1255 1180 1315 1635 1977 1258 17 153
Cuts at: 9115 9300 9745 325
Size: 185 445 1498
Fragments arranged by size:
1977 1635 1498 1315 1258 1255 1180 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2275 5485 9164
Size: 274 80 33 51 1443 298 3210 3679
Cuts at: 9164 10876 96
Size: 1712 138
Fragments arranged by size:
3679 3210 1712 1443 298 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 3175 3207 4272 4304 5433 5465 6729
Size: 32 900 32 1065 32 1129 32 1264
Cuts at: 6729 6761 6817 6849 7588 7620 10878 10910 2243
Size: 32 56 32 739 32 3258 32 2251
Fragments arranged by size:
3258 2251 1264 1129 1065 900 739 56
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10918
XbaI T'CTAG_A
Cuts at: 8254 8254
Size: 10918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2987 3695 4408 5395 7984 2167
Size: 820 708 713 987 2589 5101
Fragments arranged by size:
5101 2589 987 820 713 708
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10918
XmaI C'CCGG_G
Cuts at: 1870 2325 2578 7708 8243 1870
Size: 455 253 5130 535 4545
Fragments arranged by size:
5130 4545 535 455 253
XmnI GAAnn'nnTTC
Cuts at: 5344 9047 5344
Size: 3703 7215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8726 276
Size: 53 83 186 936 7192 2468
Fragments arranged by size:
7192 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SwaI