Restriction map for
pFN21ASDA0173
(Circular) (Six-base) MAPSORT of: pFN21ASDA0173.seq Check: 3859 from: 1 to: 8326
pFN21ASDA0173 8326 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6136 278
Size: 53 83 186 936 4600 2468
Fragments arranged by size:
4600 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 5669 1937
Size: 948 2784 4594
Fragments arranged by size:
4594 2784 948
Acc65I G'GTAC_C
Cuts at: 5647 5647
Size: 8326
AclI AA'CG_TT
Cuts at: 1563 3768 6453 6826 1563
Size: 2205 2685 373 3063
Fragments arranged by size:
3063 2685 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2583 2759 3567 4550 5323 5424 6389 7773
Size: 951 176 808 983 773 101 965 1384
Cuts at: 7773 8041 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 983 965 951 808 773 268
176 101
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8129
AflIII A'CryG_T
Cuts at: 1203 7225 8202 1203
Size: 6022 977 1327
Fragments arranged by size:
6022 1327 977
AgeI A'CCGG_T
Cuts at: 7157 7157
Size: 8326
AhdI GACnn_n'nnGTC
Cuts at: 7055 7055
Size: 8326
AleI CACnn'nnGTG
Cuts at: 1166 2708 1166
Size: 1542 6784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2726 4962 1184
Size: 1542 2236 4548
Fragments arranged by size:
4548 2236 1542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 8294
AlwNI CAG_nnn'CTG
Cuts at: 2108 2145 2328 2413 2585 2737 3518 4875 6069
Size: 37 183 85 172 152 781 1357 1194
Cuts at: 6069 7641 2108
Size: 1572 2793
Fragments arranged by size:
2793 1572 1357 1194 781 183 172 152
85 37
ApaI G_GGCC'C
Cuts at: 2228 2228
Size: 8326
ApaLI G'TGCA_C
Cuts at: 2805 6383 7539 2805
Size: 3578 1156 3592
Fragments arranged by size:
3592 3578 1156
ApoI r'AATT_y
Cuts at: 1493 3696 4986 5097 5635 5861 6013 8122 1493
Size: 2203 1290 111 538 226 152 2109 1697
Fragments arranged by size:
2203 2109 1697 1290 538 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 6880 160
Size: 872 5848 1606
Fragments arranged by size:
5848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8326
AvaI C'yCGr_G
Cuts at: 1870 1943 3795 4200 5651 1870
Size: 73 1852 405 1451 4545
Fragments arranged by size:
4545 1852 1451 405 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4256 4289 4256
Size: 33 8293
BamHI G'GATC_C
Cuts at: 3007 5656 6023 3007
Size: 2649 367 5310
Fragments arranged by size:
5310 2649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2830 3227 5647 7102 618
Size: 325 217 627 1043 397 2420 1455 1842
Fragments arranged by size:
2420 1842 1455 1043 627 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2228 2441 3315 4632 5508 5618 5645 1391
Size: 837 213 874 1317 876 110 27 4072
Fragments arranged by size:
4072 1317 876 874 837 213 110 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2515 3253 3588 5412 5438 928
Size: 971 616 738 335 1824 26 3816
Fragments arranged by size:
3816 1824 971 738 616 335 26
BbvCI CC'TCA_GC
Cuts at: 2029 3352 2029
Size: 1323 7003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6517 6551 1903
Size: 34 4580 34 3678
Fragments arranged by size:
4580 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6218 7428 6218
Size: 1210 7116
BclI T'GATC_A
Cuts at: 1548 4993 5535 1548
Size: 3445 542 4339
Fragments arranged by size:
4339 3445 542
BfrBI ATG'CAT
Cuts at: 5009 5009
Size: 8326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 2504 2960
Size: 107 122 71 631 605 566 266 456
Cuts at: 2960 3416 4077 6937 136
Size: 456 661 2860 1525
Fragments arranged by size:
2860 1525 661 631 605 566 456 456
266 122 107 71
BglII A'GATC_T
Cuts at: 5215 8321 5215
Size: 3106 5220
BlpI GC'TnA_GC
Cuts at: 5743 5743
Size: 8326
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2809 2835 2872 4528 6387 7543 1790
Size: 438 581 26 37 1656 1859 1156 2573
Fragments arranged by size:
2573 1859 1656 1156 581 438 37 26
BmgBI CAC'GTC
Cuts at: 1318 5323 1318
Size: 4005 4321
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4099 5463 6057
Size: 416 53 83 399 24 2682 1364 594
Cuts at: 6057 7015 442
Size: 958 1753
Fragments arranged by size:
2682 1753 1364 958 594 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2895 1056
Size: 1839 6487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3505 3640 3652 3505
Size: 135 12 8179
Fragments arranged by size:
8179 135 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2368 2435 2817 3360 3509 4750 4917 5259
Size: 1129 67 382 543 149 1241 167 342
Cuts at: 5259 6986 1239
Size: 1727 2579
Fragments arranged by size:
2579 1727 1241 1129 543 382 342 167
149 67
Bpu10I CC'TnA_GC
Cuts at: 2029 3352 4550 2029
Size: 1323 1198 5805
Fragments arranged by size:
5805 1323 1198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4634 4706 5421 6451 7316 7614 4634
Size: 72 715 1030 865 298 5346
Fragments arranged by size:
5346 1030 865 715 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3457 5289 6989 882
Size: 617 1958 1832 1700 2219
Fragments arranged by size:
2219 1958 1832 1700 617
BsaAI yAC'GTr
Cuts at: 493 1204 4061 5623 6117 493
Size: 711 2857 1562 494 2702
Fragments arranged by size:
2857 2702 1562 711 494
BsaBI GATnn'nnATC
Cuts at: 8320 8320
Size: 8326
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 4128 4575 6133
Size: 53 83 186 936 708 1887 447 1558
Cuts at: 6133 6515 7150 275
Size: 382 635 1451
Fragments arranged by size:
1887 1558 1451 936 708 635 447 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2940 5223 6759 7157 7431 7578 7989 2940
Size: 2283 1536 398 274 147 411 3277
Fragments arranged by size:
3277 2283 1536 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 2328 2358 2366
Size: 30 165 30 792 30 103 30 8
Cuts at: 2366 2396 3011 3041 1178
Size: 30 615 30 6463
Fragments arranged by size:
6463 792 615 165 103 30 30 30
30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2539 3340 3485 3625 3626 3629 5107 1186
Size: 1353 801 145 140 1 3 1478 4405
Fragments arranged by size:
4405 1478 1353 801 145 140 3 1
BseYI C'CCAG_C
Cuts at: 2250 2273 2535 2585 2854 3119 3442 3656 3934
Size: 23 262 50 269 265 323 214 278
Cuts at: 3934 3942 4868 5362 5507 7529 8025 2250
Size: 8 926 494 145 2022 496 2551
Fragments arranged by size:
2551 2022 926 496 494 323 278 269
265 262 214 145 50 23 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4932 1163
Size: 573 3196 4557
Fragments arranged by size:
4557 3196 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5776 6537 7565 1540
Size: 275 186 3775 761 1028 2301
Fragments arranged by size:
3775 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2809 2983 3635 3860 5546 6387 6472 7543 2809
Size: 174 652 225 1686 841 85 1071 3592
Fragments arranged by size:
3592 1686 1071 841 652 225 174 85
BsmI GAATG_Cn'
Cuts at: 3282 4477 5837 5930 3282
Size: 1195 1360 93 5678
Fragments arranged by size:
5678 1360 1195 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2441 2809 2835 2872 2983 3315
Size: 399 438 213 368 26 37 111 332
Cuts at: 3315 3635 3860 4528 4632 5508 5546 5618 5645
Size: 320 225 668 104 876 38 72 27
Cuts at: 5645 6387 6472 7543 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 876 742 668 438 399 368
332 320 225 213 111 104 85 72
38 37 27 26
BspEI T'CCGG_A
Cuts at: 5223 7989 5223
Size: 2766 5560
BspHI T'CATG_A
Cuts at: 3721 6215 7945 3721
Size: 2494 1730 4102
Fragments arranged by size:
4102 2494 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 4323 4817 5681 844
Size: 1272 2207 494 864 3489
Fragments arranged by size:
3489 2207 1272 864 494
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 6213 1394
Size: 429 559 3831 3507
Fragments arranged by size:
3831 3507 559 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2307 6821 6995 65
Size: 1183 1059 4514 174 1396
Fragments arranged by size:
4514 1396 1183 1059 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4038 6970 7157 1700
Size: 110 2228 2932 187 2869
Fragments arranged by size:
2932 2869 2228 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8326
BssSI C'ACGA_G
Cuts at: 6386 7398 6386
Size: 1012 7314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8326
BstBI TT'CG_AA
Cuts at: 2007 7130 2007
Size: 5123 3203
BstEII G'GTnAC_C
Cuts at: 1184 3947 1184
Size: 2763 5563
BstXI CCAn_nnnn'nTGG
Cuts at: 3442 3484 3442
Size: 42 8284
BstYI r'GATC_y
Cuts at: 1972 2634 2760 3007 4320 5215 5656 6023 6408
Size: 662 126 247 1313 895 441 367 385
Cuts at: 6408 6425 7877 7963 7975 8321 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1313 895 662 441 385 367
346 247 126 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2095 2151 3189 3468 2095
Size: 56 1038 279 6953
Fragments arranged by size:
6953 1038 279 56
BtgI C'CryG_G
Cuts at: 513 1065 2022 2298 2517 2529 2541 5080 5191
Size: 552 957 276 219 12 12 2539 111
Cuts at: 5191 513
Size: 3648
Fragments arranged by size:
3648 2539 957 552 276 219 111 12
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7587
Fragments arranged by size:
7587 683 56
BtsI GCAGTG_nn'
Cuts at: 2493 4944 5842 6636 6656 2493
Size: 2451 898 794 20 4163
Fragments arranged by size:
4163 2451 898 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2068 2103 2902 2937 529
Size: 35 1504 35 799 35 5918
Fragments arranged by size:
5918 1504 799 35 35 35
DraI TTT'AAA
Cuts at: 5630 5630
Size: 8326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3257 7333 809
Size: 507 1941 4076 1802
Fragments arranged by size:
4076 1941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2693 3417 3669
Size: 54 1425 50 275 18 863 724 252
Cuts at: 3669 5773 6662 8291 8
Size: 2104 889 1629 43
Fragments arranged by size:
2104 1629 1425 889 863 724 275 252
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5773 1537
Size: 275 3961 4090
Fragments arranged by size:
4090 3961 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 2656 2932 3436 3603 4412 6256 1353
Size: 1247 56 276 504 167 809 1844 3423
Fragments arranged by size:
3423 1844 1247 809 504 276 167 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 4063 6913 7287 7433 387
Size: 1064 138 462 2012 2850 374 146 1280
Fragments arranged by size:
2850 2012 1280 1064 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2368 2435 2583 2759 2817 3360 3509
Size: 393 736 67 148 176 58 543 149
Cuts at: 3509 3567 4550 4750 4917 5259 5323 5424 6389
Size: 58 983 200 167 342 64 101 965
Cuts at: 6389 6986 7773 8041 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 983 965 787 736 597 543 393
342 268 200 176 167 149 148 101
67 64 58 58
EcoNI CCTnn'n_nnAGG
Cuts at: 4121 4121
Size: 8326
EcoO109I rG'GnC_Cy
Cuts at: 2734 3217 3380 4254 4732 2734
Size: 483 163 874 478 6328
Fragments arranged by size:
6328 874 483 478 163
EcoRI G'AATT_C
Cuts at: 5635 6013 5635
Size: 378 7948
EcoRV GAT'ATC
Cuts at: 4783 4855 4783
Size: 72 8254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5462 5462
Size: 8326
FspI TGC'GCA
Cuts at: 4142 6832 4142
Size: 2690 5636
HaeII r_GCGC'y
Cuts at: 1678 7473 1678
Size: 5795 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2626 2658 2978
Size: 32 135 32 623 32 399 32 320
Cuts at: 2978 3010 3188 3220 3729 3761 6967 6999 7041
Size: 32 178 32 509 32 3206 32 42
Cuts at: 7041 7073 1373
Size: 32 2626
Fragments arranged by size:
3206 2626 623 509 399 320 178 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2821 4744 5670 5916 669
Size: 988 281 883 1923 926 246 3079
Fragments arranged by size:
3079 1923 988 926 883 281 246
HindIII A'AGCT_T
Cuts at: 748 5201 748
Size: 4453 3873
HpaI GTT'AAC
Cuts at: 5916 5916
Size: 8326
KpnI G_GTAC'C
Cuts at: 5651 5651
Size: 8326
MfeI C'AATT_G
Cuts at: 5925 5925
Size: 8326
MluI A'CGCG_T
Cuts at: 8202 8202
Size: 8326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3035 3228 3760 7440 7624 1305
Size: 1730 193 532 3680 184 2007
Fragments arranged by size:
3680 2007 1730 532 193 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2695 3419 3671 10
Size: 54 1425 343 863 724 252 4665
Fragments arranged by size:
4665 1425 863 724 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 3306 6284 6643 6802 518
Size: 648 1542 598 2978 359 159 2042
Fragments arranged by size:
2978 2042 1542 648 598 359 159
MspA1I CmG'CkG
Cuts at: 1606 2582 2858 3442 4299 4875 5742 6419 7567
Size: 976 276 584 857 576 867 677 1148
Cuts at: 7567 7812 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 976 867 857 677 584 576
276 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2517 2529 2541 5191 513
Size: 552 957 495 12 12 2650 3648
Fragments arranged by size:
3648 2650 957 552 495 12 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8326
NheI G'CTAG_C
Cuts at: 1052 2891 1052
Size: 1839 6487
NotI GC'GGCC_GC
Cuts at: 5773 5773
Size: 8326
NsiI A_TGCA'T
Cuts at: 5011 5011
Size: 8326
NspI r_CATG'y
Cuts at: 1132 1229 3305 4943 5684 7229 1132
Size: 97 2076 1638 741 1545 2229
Fragments arranged by size:
2229 2076 1638 1545 741 97
PasI CC'CwG_GG
Cuts at: 1348 3822 4728 4910 1348
Size: 2474 906 182 4764
Fragments arranged by size:
4764 2474 906 182
PciI A'CATG_T
Cuts at: 7225 7225
Size: 8326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2413 2542 5175 1108
Size: 1305 129 2633 4259
Fragments arranged by size:
4259 2633 1305 129
PfoI T'CCnGG_A
Cuts at: 2346 4734 2346
Size: 2388 5938
PmeI GTTT'AAAC
Cuts at: 5630 5630
Size: 8326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2553 2585 3011 3043 6395 6427 7908 7940 2553
Size: 32 426 32 3352 32 1481 32 2939
Fragments arranged by size:
3352 2939 1481 426 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3217 3380 4732 3217
Size: 163 1352 6811
Fragments arranged by size:
6811 1352 163
PshAI GACnn'nnGTC
Cuts at: 1613 4920 1613
Size: 3307 5019
PsiI TTA'TAA
Cuts at: 5896 5896
Size: 8326
PspOMI G'GGCC_C
Cuts at: 2224 2224
Size: 8326
PspXI vC'TCGA_Gb
Cuts at: 4200 4200
Size: 8326
PstI C_TGCA'G
Cuts at: 830 4880 5678 830
Size: 4050 798 3478
Fragments arranged by size:
4050 3478 798
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8326
PvuII CAG'CTG
Cuts at: 2858 3442 4875 2858
Size: 584 1433 6309
Fragments arranged by size:
6309 1433 584
SalI G'TCGA_C
Cuts at: 1936 5668 1936
Size: 3732 4594
SapI GCTCTTCn'nnn_
Cuts at: 2600 4412 2600
Size: 1812 6514
SbfI CC_TGCA'GG
Cuts at: 5678 5678
Size: 8326
ScaI AGT'ACT
Cuts at: 6574 8286 6574
Size: 1712 6614
SfcI C'TryA_G
Cuts at: 826 1045 2047 2389 3751 4453 4672 4876 5674
Size: 219 1002 342 1362 702 219 204 798
Cuts at: 5674 6809 7490 7681 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1362 1135 1002 798 702 681 342
219 219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8326
SmaI CCC'GGG
Cuts at: 1872 5653 1872
Size: 3781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4200 4649 4721 5436 6430 7331
Size: 197 926 2257 449 72 715 994 901
Cuts at: 7331 7593 820
Size: 262 1553
Fragments arranged by size:
2257 1553 994 926 901 715 449 262
197 72
SnaBI TAC'GTA
Cuts at: 493 5623 6117 493
Size: 5130 494 2702
Fragments arranged by size:
5130 2702 494
SpeI A'CTAG_T
Cuts at: 152 3565 152
Size: 3413 4913
SphI G_CATG'C
Cuts at: 1132 5684 1132
Size: 4552 3774
SspI AAT'ATT
Cuts at: 5 52 6250 5
Size: 47 6198 2081
Fragments arranged by size:
6198 2081 47
StuI AGG'CCT
Cuts at: 1517 2232 1517
Size: 715 7611
StyI C'CwwG_G
Cuts at: 513 1065 2022 2517 2529 2541 3240 4423 5191
Size: 552 957 495 12 12 699 1183 768
Cuts at: 5191 5765 513
Size: 574 3074
Fragments arranged by size:
3074 1183 957 768 699 574 552 495
12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2770 3196 5186 6353 6370 6523 6708
Size: 1255 1190 426 1990 1167 17 153 185
Cuts at: 6708 7153 325
Size: 445 1498
Fragments arranged by size:
1990 1498 1255 1190 1167 445 426 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6572 8284 96
Size: 274 80 33 51 1443 4595 1712 138
Fragments arranged by size:
4595 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 2745 2777 2891 2923 8286 8318 2309
Size: 32 404 32 114 32 5363 32 2317
Fragments arranged by size:
5363 2317 404 114 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2209 1316
Size: 893 7433
XbaI T'CTAG_A
Cuts at: 3680 5662 3680
Size: 1982 6344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2515 3664 4688 4774 2515
Size: 1149 1024 86 6067
Fragments arranged by size:
6067 1149 1024 86
XhoI C'TCGA_G
Cuts at: 1943 4200 1943
Size: 2257 6069
XmaI C'CCGG_G
Cuts at: 1870 5651 1870
Size: 3781 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 6455 4088
Size: 2367 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6134 276
Size: 53 83 186 936 4600 2468
Fragments arranged by size:
4600 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I
DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI