Restriction map for
pFN21ASDA0172
(Circular) (Six-base) MAPSORT of: pFN21ASDA0172.seq Check: 6683 from: 1 to: 8785
pFN21ASDA0172 8785 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2057 2808 2851 2057
Size: 751 43 7991
Fragments arranged by size:
7991 751 43
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6047 6595 278
Size: 53 83 186 936 4511 548 2468
Fragments arranged by size:
4511 2468 936 548 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6128 1937
Size: 4191 4594
Acc65I G'GTAC_C
Cuts at: 6106 6106
Size: 8785
AclI AA'CG_TT
Cuts at: 1563 4241 6912 7285 1563
Size: 2678 2671 373 3063
Fragments arranged by size:
3063 2678 2671 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2864 3390 4689 4742 4964 4988 6848
Size: 1010 222 526 1299 53 222 24 1860
Cuts at: 6848 8232 8500 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1860 1384 1299 1010 526 268 222
222 53 24
AflII C'TTAA_G
Cuts at: 820 1017 4774 820
Size: 197 3757 4831
Fragments arranged by size:
4831 3757 197
AflIII A'CryG_T
Cuts at: 1203 3627 3629 7684 8661 1203
Size: 2424 2 4055 977 1327
Fragments arranged by size:
4055 2424 1327 977 2
AgeI A'CCGG_T
Cuts at: 7616 7616
Size: 8785
AhdI GACnn_n'nnGTC
Cuts at: 7514 7514
Size: 8785
AleI CACnn'nnGTG
Cuts at: 1166 2774 1166
Size: 1608 7177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8785
AlwNI CAG_nnn'CTG
Cuts at: 3177 3285 3819 4175 4519 4540 6528 8100 3177
Size: 108 534 356 344 21 1988 1572 3862
Fragments arranged by size:
3862 1988 1572 534 356 344 108 21
ApaI G_GGCC'C
Cuts at: 3037 3037
Size: 8785
ApaLI G'TGCA_C
Cuts at: 4061 6842 7998 4061
Size: 2781 1156 4848
Fragments arranged by size:
4848 2781 1156
ApoI r'AATT_y
Cuts at: 1493 6094 6320 6472 8581 1493
Size: 4601 226 152 2109 1697
Fragments arranged by size:
4601 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5019 7339 160
Size: 872 3987 2320 1606
Fragments arranged by size:
3987 2320 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8785
AvaI C'yCGr_G
Cuts at: 1870 1943 3030 3963 5151 6110 1870
Size: 73 1087 933 1188 959 4545
Fragments arranged by size:
4545 1188 1087 959 933 73
AvrII C'CTAG_G
Cuts at: 6026 6026
Size: 8785
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2659 2692 2659
Size: 33 8752
BamHI G'GATC_C
Cuts at: 6115 6482 6115
Size: 367 8418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 3233 5169 6020 6106
Size: 325 217 627 807 639 1936 851 86
Cuts at: 6106 7561 618
Size: 1455 1842
Fragments arranged by size:
1936 1842 1455 851 807 639 627 325
217 86
BanII G_rGCy'C
Cuts at: 1391 2735 2759 2792 3037 3198 3393 4604 5825
Size: 1344 24 33 245 161 195 1211 1221
Cuts at: 5825 6104 1391
Size: 279 4072
Fragments arranged by size:
4072 1344 1221 1211 279 245 195 161
33 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2518 4292 5151 6047 928
Size: 971 619 1774 859 896 3666
Fragments arranged by size:
3666 1774 971 896 859 619
BbvCI CC'TCA_GC
Cuts at: 4676 4676
Size: 8785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6976 7010 1903
Size: 34 5039 34 3678
Fragments arranged by size:
5039 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2660 4520 5121 6677 7887 2660
Size: 1860 601 1556 1210 3558
Fragments arranged by size:
3558 1860 1556 1210 601
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8785
BfrBI ATG'CAT
Cuts at: 3754 4919 3754
Size: 1165 7620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3539 4010 5792
Size: 107 122 71 631 605 1867 471 1782
Cuts at: 5792 7396 136
Size: 1604 1525
Fragments arranged by size:
1867 1782 1604 1525 631 605 471 122
107 71
BglII A'GATC_T
Cuts at: 2892 3426 8780 2892
Size: 534 5354 2897
Fragments arranged by size:
5354 2897 534
BlpI GC'TnA_GC
Cuts at: 6202 6202
Size: 8785
Bme1580I G_kGCm'C
Cuts at: 1790 2333 2597 3037 4065 5172 5859 6023 6846
Size: 543 264 440 1028 1107 687 164 823
Cuts at: 6846 8002 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1107 1028 823 687 543 440
264 164
BmgBI CAC'GTC
Cuts at: 1318 3931 1318
Size: 2613 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2768 6004 6516
Size: 416 53 83 399 24 1351 3236 512
Cuts at: 6516 7474 442
Size: 958 1753
Fragments arranged by size:
3236 1753 1351 958 512 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3976 3205
Size: 771 8014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2867 2873 3173 3262 4466 4860 5360
Size: 1178 450 6 300 89 1204 394 500
Cuts at: 5360 7445 1239
Size: 2085 2579
Fragments arranged by size:
2579 2085 1204 1178 500 450 394 300
89 6
Bpu10I CC'TnA_GC
Cuts at: 2753 2758 3197 3708 4676 4797 2753
Size: 5 439 511 968 121 6741
Fragments arranged by size:
6741 968 511 439 121 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3854 4446 6910 7775 8073 3854
Size: 592 2464 865 298 4566
Fragments arranged by size:
4566 2464 865 592 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 2117 2393 2495 2932 3080 3585
Size: 617 580 38 276 102 437 148 505
Cuts at: 3585 4346 7448 882
Size: 761 3102 2219
Fragments arranged by size:
3102 2219 761 617 580 505 437 276
148 102 38
BsaAI yAC'GTr
Cuts at: 493 1204 3630 5860 6082 6576 493
Size: 711 2426 2230 222 494 2702
Fragments arranged by size:
2702 2426 2230 711 494 222
BsaBI GATnn'nnATC
Cuts at: 8779 8779
Size: 8785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6044 6592 6974 7609
Size: 53 83 186 936 4511 548 382 635
Cuts at: 7609 275
Size: 1451
Fragments arranged by size:
4511 1451 936 635 548 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7218 7616 7890 8037 8448 7218
Size: 398 274 147 411 7555
Fragments arranged by size:
7555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2353 2383 2445 2475 3260
Size: 30 165 30 950 30 62 30 785
Cuts at: 3260 3290 4190 4220 4858 4888 5807 5837 1178
Size: 30 900 30 638 30 919 30 4126
Fragments arranged by size:
4126 950 919 900 785 638 165 62
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 3086 3178 3254 3857 4198 5141 5144
Size: 1684 216 92 76 603 341 943 3
Cuts at: 5144 5147 5150 1186
Size: 3 3 4821
Fragments arranged by size:
4821 1684 943 603 341 216 92 76
3 3 3
BseYI C'CCAG_C
Cuts at: 2701 3012 3543 3767 4455 4791 5886 7988 8484
Size: 311 531 224 688 336 1095 2102 496
Cuts at: 8484 2701
Size: 3002
Fragments arranged by size:
3002 2102 1095 688 531 496 336 311
224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3602 4090 1163
Size: 573 1866 488 5858
Fragments arranged by size:
5858 1866 573 488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6235 6996 8024 1540
Size: 275 186 4234 761 1028 2301
Fragments arranged by size:
4234 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2735 2759 2883 3104 3198 3393 4065 5853 6846
Size: 24 124 221 94 195 672 1788 993
Cuts at: 6846 6931 8002 2735
Size: 85 1071 3518
Fragments arranged by size:
3518 1788 1071 993 672 221 195 124
94 85 24
BsmI GAATG_Cn'
Cuts at: 5707 6296 6389 5707
Size: 589 93 8103
Fragments arranged by size:
8103 589 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4076 4154 6051 4076
Size: 78 1897 6810
Fragments arranged by size:
6810 1897 78
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2597 2735 2759 2792 2883 3037
Size: 399 543 264 138 24 33 91 154
Cuts at: 3037 3104 3198 3393 4065 4604 5172 5825 5853
Size: 67 94 195 672 539 568 653 28
Cuts at: 5853 5859 6023 6104 6846 6931 8002 1391
Size: 6 164 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 653 568 543 539
399 264 195 164 154 138 94 91
85 81 67 33 28 24 6
BspEI T'CCGG_A
Cuts at: 8448 8448
Size: 8785
BspHI T'CATG_A
Cuts at: 4650 4953 6674 8404 4650
Size: 303 1721 1730 5031
Fragments arranged by size:
5031 1730 1721 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2808 2851 5215 6140 844
Size: 1213 751 43 2364 925 3489
Fragments arranged by size:
3489 2364 1213 925 751 43
BsrBI CCG'CTC
Cuts at: 1394 1823 6672 1394
Size: 429 4849 3507
Fragments arranged by size:
4849 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 7280 7454 65
Size: 1183 1578 4454 174 1396
Fragments arranged by size:
4454 1578 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3216 7429 7616 1700
Size: 110 1406 4213 187 2869
Fragments arranged by size:
4213 2869 1406 187 110
BsrGI T'GTAC_A
Cuts at: 96 4922 96
Size: 4826 3959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8785
BssSI C'ACGA_G
Cuts at: 5381 6845 7857 5381
Size: 1464 1012 6309
Fragments arranged by size:
6309 1464 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3672 4540 794
Size: 2878 868 5039
Fragments arranged by size:
5039 2878 868
BstBI TT'CG_AA
Cuts at: 2007 7589 2007
Size: 5582 3203
BstEII G'GTnAC_C
Cuts at: 1184 5903 1184
Size: 4719 4066
BstXI CCAn_nnnn'nTGG
Cuts at: 2678 5626 2678
Size: 2948 5837
BstYI r'GATC_y
Cuts at: 1972 2763 2892 3162 3426 6115 6482 6867 6884
Size: 791 129 270 264 2689 367 385 17
Cuts at: 6884 8336 8422 8434 8780 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2689 1977 1452 791 385 367 346 270
264 129 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2056 2056
Size: 8785
BtgI C'CryG_G
Cuts at: 513 1065 2022 2724 3645 5424 5826 513
Size: 552 957 702 921 1779 402 3472
Fragments arranged by size:
3472 1779 957 921 702 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5953 487
Size: 683 56 4727 3319
Fragments arranged by size:
4727 3319 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2333 3582 4020 4538 5928 6301 7095 7115
Size: 277 1249 438 518 1390 373 794 20
Cuts at: 7115 2056
Size: 3726
Fragments arranged by size:
3726 1390 1249 794 518 438 373 277
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2953 2988 4359 4394 529
Size: 35 2389 35 1371 35 4920
Fragments arranged by size:
4920 2389 1371 35 35 35
DraI TTT'AAA
Cuts at: 6089 6089
Size: 8785
DraIII CAC_nnn'GTG
Cuts at: 2552 2552
Size: 8785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3599 3935 4294 7792 809
Size: 507 2283 336 359 3498 1802
Fragments arranged by size:
3498 2283 1802 507 359 336
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 2823 4086
Size: 54 1425 50 275 18 744 249 1263
Cuts at: 4086 4252 6232 7121 8750 8
Size: 166 1980 889 1629 43
Fragments arranged by size:
1980 1629 1425 1263 889 744 275 249
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6232 1537
Size: 275 4420 4090
Fragments arranged by size:
4420 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 2904 4665 5077 5118 6715 1353
Size: 857 694 1761 412 41 1597 3423
Fragments arranged by size:
3423 1761 1597 857 694 412 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2236 2729 7372 7746 7892 387
Size: 1064 138 647 493 4643 374 146 1280
Fragments arranged by size:
4643 1280 1064 647 493 374 146 138
EcoICRI GAG'CTC
Cuts at: 2733 2757 3196 3391 2733
Size: 24 439 195 8127
Fragments arranged by size:
8127 439 195 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2642 2864 2867 2873 3173 3262
Size: 393 785 225 222 3 6 300 89
Cuts at: 3262 3390 4466 4689 4742 4860 4964 4988 5360
Size: 128 1076 223 53 118 104 24 372
Cuts at: 5360 6848 7445 8232 8500 1239
Size: 1488 597 787 268 1524
Fragments arranged by size:
1524 1488 1076 787 785 597 393 372
300 268 225 223 222 128 118 104
89 53 24 6 3
EcoO109I rG'GnC_Cy
Cuts at: 3282 3639 3704 4753 4812 5776 3282
Size: 357 65 1049 59 964 6291
Fragments arranged by size:
6291 1049 964 357 65 59
EcoRI G'AATT_C
Cuts at: 6094 6472 6094
Size: 378 8407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3464 3895 4487 5091 3464
Size: 431 592 604 7158
Fragments arranged by size:
7158 604 592 431
FspI TGC'GCA
Cuts at: 7291 7291
Size: 8785
HaeII r_GCGC'y
Cuts at: 1678 7932 1678
Size: 6254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2193 2225 3203 3235 4190
Size: 32 135 32 621 32 978 32 955
Cuts at: 4190 4222 4259 4291 5042 5054 5074 5086 5347
Size: 32 37 32 751 12 20 12 261
Cuts at: 5347 5379 5807 5839 7426 7458 7500 7532 1373
Size: 32 428 32 1587 32 42 32 2626
Fragments arranged by size:
2626 1587 978 955 751 621 428 261
135 42 37 32 32 32 32 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2044 3526 3928 4380 5803 5995
Size: 988 281 106 1482 402 452 1423 192
Cuts at: 5995 6129 6375 669
Size: 134 246 3079
Fragments arranged by size:
3079 1482 1423 988 452 402 281 246
192 134 106
HindIII A'AGCT_T
Cuts at: 748 4572 5334 748
Size: 3824 762 4199
Fragments arranged by size:
4199 3824 762
HpaI GTT'AAC
Cuts at: 2044 6375 2044
Size: 4331 4454
KpnI G_GTAC'C
Cuts at: 6110 6110
Size: 8785
MfeI C'AATT_G
Cuts at: 6384 6384
Size: 8785
MluI A'CGCG_T
Cuts at: 8661 8661
Size: 8785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 3418 4216 5565 7899 8083 1305
Size: 1026 1087 798 1349 2334 184 2007
Fragments arranged by size:
2334 2007 1349 1087 1026 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 2825 4254 10
Size: 54 1425 343 744 249 1429 4541
Fragments arranged by size:
4541 1429 1425 744 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 2774 2876 3260 4013 6743 7102
Size: 648 1374 234 102 384 753 2730 359
Cuts at: 7102 7261 518
Size: 159 2042
Fragments arranged by size:
2730 2042 1374 753 648 384 359 234
159 102
MspA1I CmG'CkG
Cuts at: 1606 2440 2705 2935 3016 3177 3299 4280 4907
Size: 834 265 230 81 161 122 981 627
Cuts at: 4907 5642 6201 6878 8026 8271 1606
Size: 735 559 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 834 735 677 627 559
265 245 230 161 122 81
NcoI C'CATG_G
Cuts at: 513 1065 2022 2724 5424 513
Size: 552 957 702 2700 3874
Fragments arranged by size:
3874 2700 957 702 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8785
NotI GC'GGCC_GC
Cuts at: 6232 6232
Size: 8785
NsiI A_TGCA'T
Cuts at: 3756 4921 3756
Size: 1165 7620
NspI r_CATG'y
Cuts at: 1132 1229 3145 4923 5301 5665 6143 7688 1132
Size: 97 1916 1778 378 364 478 1545 2229
Fragments arranged by size:
2229 1916 1778 1545 478 378 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8785
PciI A'CATG_T
Cuts at: 7684 7684
Size: 8785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4156 1108
Size: 3048 5737
PfoI T'CCnGG_A
Cuts at: 3165 5808 3165
Size: 2643 6142
PmeI GTTT'AAAC
Cuts at: 6089 6089
Size: 8785
PmlI CAC'GTG
Cuts at: 3630 5860 3630
Size: 2230 6555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 6854 6886 8367 8399 2011
Size: 32 4811 32 1481 32 2397
Fragments arranged by size:
4811 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3282 3639 4812 3282
Size: 357 1173 7255
Fragments arranged by size:
7255 1173 357
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8785
PsiI TTA'TAA
Cuts at: 6355 6355
Size: 8785
PspOMI G'GGCC_C
Cuts at: 3033 3033
Size: 8785
PstI C_TGCA'G
Cuts at: 830 2805 3501 4030 6137 830
Size: 1975 696 529 2107 3478
Fragments arranged by size:
3478 2107 1975 696 529
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8785
PvuII CAG'CTG
Cuts at: 2440 2705 2935 3016 3177 3299 4280 2440
Size: 265 230 81 161 122 981 6945
Fragments arranged by size:
6945 981 265 230 161 122 81
SacI G_AGCT'C
Cuts at: 2735 2759 3198 3393 2735
Size: 24 439 195 8127
Fragments arranged by size:
8127 439 195 24
SalI G'TCGA_C
Cuts at: 1936 6127 1936
Size: 4191 4594
SapI GCTCTTCn'nnn_
Cuts at: 4665 5077 4665
Size: 412 8373
SbfI CC_TGCA'GG
Cuts at: 6137 6137
Size: 8785
ScaI AGT'ACT
Cuts at: 7033 8745 7033
Size: 1712 7073
SexAI A'CCwGG_T
Cuts at: 3618 3618
Size: 8785
SfcI C'TryA_G
Cuts at: 826 1045 2801 2992 3300 3497 4026 4176 4356
Size: 219 1756 191 308 197 529 150 180
Cuts at: 4356 4431 4555 6133 7268 7949 8140 826
Size: 75 124 1578 1135 681 191 1471
Fragments arranged by size:
1756 1578 1471 1135 681 529 308 219
197 191 191 180 150 124 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8785
SmaI CCC'GGG
Cuts at: 1872 3032 3965 6112 1872
Size: 1160 933 2147 4545
Fragments arranged by size:
4545 2147 1160 933
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3869 4461 4774 6889 7790 8052
Size: 197 926 1926 592 313 2115 901 262
Cuts at: 8052 820
Size: 1553
Fragments arranged by size:
2115 1926 1553 926 901 592 313 262
197
SnaBI TAC'GTA
Cuts at: 493 6082 6576 493
Size: 5589 494 2702
Fragments arranged by size:
5589 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8785
SphI G_CATG'C
Cuts at: 1132 5301 6143 1132
Size: 4169 842 3774
Fragments arranged by size:
4169 3774 842
SspI AAT'ATT
Cuts at: 5 52 4318 5185 6709 5
Size: 47 4266 867 1524 2081
Fragments arranged by size:
4266 2081 1524 867 47
StuI AGG'CCT
Cuts at: 1517 3185 1517
Size: 1668 7117
StyI C'CwwG_G
Cuts at: 513 1065 2022 2671 2724 5424 6026 6224 513
Size: 552 957 649 53 2700 602 198 3074
Fragments arranged by size:
3074 2700 957 649 602 552 198 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3221 3476 3774 4163 6074 6812 6829
Size: 1255 1641 255 298 389 1911 738 17
Cuts at: 6829 6982 7167 7612 325
Size: 153 185 445 1498
Fragments arranged by size:
1911 1641 1498 1255 738 445 389 298
255 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3442 4922 4937
Size: 274 80 33 51 1443 1465 1480 15
Cuts at: 4937 7031 8743 96
Size: 2094 1712 138
Fragments arranged by size:
2094 1712 1480 1465 1443 274 138 80
51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2445 2477 3260 3292 8745 8777 2445
Size: 32 783 32 5453 32 2453
Fragments arranged by size:
5453 2453 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3257 4222 1316
Size: 1941 965 5879
Fragments arranged by size:
5879 1941 965
XbaI T'CTAG_A
Cuts at: 6121 6121
Size: 8785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2660 2722 3595 4904 5422 2494
Size: 166 62 873 1309 518 5857
Fragments arranged by size:
5857 1309 873 518 166 62
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8785
XmaI C'CCGG_G
Cuts at: 1870 3030 3963 6110 1870
Size: 1160 933 2147 4545
Fragments arranged by size:
4545 2147 1160 933
XmnI GAAnn'nnTTC
Cuts at: 6914 6914
Size: 8785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6045 6593 276
Size: 53 83 186 936 4511 548 2468
Fragments arranged by size:
4511 2468 936 548 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BsiWI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI