Restriction map for
pFN21ASDA0171
(Circular) (Six-base) MAPSORT of: pFN21ASDA0171.seq Check: 2487 from: 1 to: 6604
pFN21ASDA0171 6604 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4414 278
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3947 1937
Size: 2010 4594
Acc65I G'GTAC_C
Cuts at: 3925 3925
Size: 6604
AclI AA'CG_TT
Cuts at: 1563 4731 5104 1563
Size: 3168 373 3063
Fragments arranged by size:
3168 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2940 2952 3267 4667 6051 6319 1632
Size: 1308 12 315 1400 1384 268 1917
Fragments arranged by size:
1917 1400 1384 1308 315 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6407
AflIII A'CryG_T
Cuts at: 1203 2031 5503 6480 1203
Size: 828 3472 977 1327
Fragments arranged by size:
3472 1327 977 828
AgeI A'CCGG_T
Cuts at: 5435 5435
Size: 6604
AhdI GACnn_n'nnGTC
Cuts at: 5333 5333
Size: 6604
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3455 1184
Size: 2271 4333
AlwNI CAG_nnn'CTG
Cuts at: 3083 3176 4347 5919 3083
Size: 93 1171 1572 3768
Fragments arranged by size:
3768 1572 1171 93
ApaLI G'TGCA_C
Cuts at: 4661 5817 4661
Size: 1156 5448
ApoI r'AATT_y
Cuts at: 1493 2432 2768 3277 3343 3877 3913 4139 4291
Size: 939 336 509 66 534 36 226 152
Cuts at: 4291 6400 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 939 534 509 336 226 152
66 36
AseI AT'TA_AT
Cuts at: 160 1032 5158 160
Size: 872 4126 1606
Fragments arranged by size:
4126 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
AvaI C'yCGr_G
Cuts at: 1870 1943 3929 1870
Size: 73 1986 4545
Fragments arranged by size:
4545 1986 73
BamHI G'GATC_C
Cuts at: 3934 4301 3934
Size: 367 6237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3017 3698 3925 5380 618
Size: 325 217 627 1230 681 227 1455 1842
Fragments arranged by size:
1842 1455 1230 681 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2562 3308 3923 1391
Size: 1171 746 615 4072
Fragments arranged by size:
4072 1171 746 615
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 2946 928
Size: 971 804 243 4586
Fragments arranged by size:
4586 971 804 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4795 4829 1903
Size: 34 2858 34 3678
Fragments arranged by size:
3678 2858 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4496 5706 4496
Size: 1210 5394
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6604
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5215 136
Size: 107 122 71 631 605 3543 1525
Fragments arranged by size:
3543 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2753 6599 2753
Size: 3846 2758
BlpI GC'TnA_GC
Cuts at: 3076 4021 3076
Size: 945 5659
Bme1580I G_kGCm'C
Cuts at: 1790 4665 5821 1790
Size: 2875 1156 2573
Fragments arranged by size:
2875 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4335 5293
Size: 416 53 83 399 24 2039 879 958
Cuts at: 5293 442
Size: 1753
Fragments arranged by size:
2039 1753 958 879 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 3166 5264 1239
Size: 1449 478 2098 2579
Fragments arranged by size:
2579 2098 1449 478
Bpu10I CC'TnA_GC
Cuts at: 2556 2556
Size: 6604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4729 5594 5892 4729
Size: 865 298 5441
Fragments arranged by size:
5441 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2822 5267 882
Size: 617 1323 2445 2219
Fragments arranged by size:
2445 2219 1323 617
BsaAI yAC'GTr
Cuts at: 493 1204 3901 4395 493
Size: 711 2697 494 2702
Fragments arranged by size:
2702 2697 711 494
BsaBI GATnn'nnATC
Cuts at: 6598 6598
Size: 6604
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4411 4793 5428 275
Size: 53 83 186 936 2878 382 635 1451
Fragments arranged by size:
2878 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2679 5037 5435 5709 5856 6267 2679
Size: 2358 398 274 147 411 3016
Fragments arranged by size:
3016 2358 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6379
Fragments arranged by size:
6379 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 2798 1186
Size: 1408 204 4992
Fragments arranged by size:
4992 1408 204
BseYI C'CCAG_C
Cuts at: 3516 3785 5807 6303 3516
Size: 269 2022 496 3817
Fragments arranged by size:
3817 2022 496 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6031
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4054 4815 5843 1540
Size: 275 186 2053 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3362 4665 4750 5821 3362
Size: 1303 85 1071 4145
Fragments arranged by size:
4145 1303 1071 85
BsmI GAATG_Cn'
Cuts at: 2935 4115 4208 2935
Size: 1180 93 5331
Fragments arranged by size:
5331 1180 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2562 3308 3362 3923 4665 4750 5821
Size: 399 772 746 54 561 742 85 1071
Cuts at: 5821 1391
Size: 2174
Fragments arranged by size:
2174 1071 772 746 742 561 399 85
54
BspEI T'CCGG_A
Cuts at: 2679 6267 2679
Size: 3588 3016
BspHI T'CATG_A
Cuts at: 4493 6223 4493
Size: 1730 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3959 844
Size: 3115 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4491 1394
Size: 429 2668 3507
Fragments arranged by size:
3507 2668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5099 5273 65
Size: 1183 3851 174 1396
Fragments arranged by size:
3851 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5248 5435 1700
Size: 110 3438 187 2869
Fragments arranged by size:
3438 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6604
BssSI C'ACGA_G
Cuts at: 4664 5676 4664
Size: 1012 5592
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6604
BstBI TT'CG_AA
Cuts at: 2007 5408 2007
Size: 3401 3203
BstEII G'GTnAC_C
Cuts at: 1184 2993 1184
Size: 1809 4795
BstXI CCAn_nnnn'nTGG
Cuts at: 3709 3709
Size: 6604
BstYI r'GATC_y
Cuts at: 1972 2753 3934 4301 4686 4703 6155 6241 6253
Size: 781 1181 367 385 17 1452 86 12
Cuts at: 6253 6599 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1181 781 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3702 513
Size: 552 2637 3415
Fragments arranged by size:
3415 2637 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2739 487
Size: 683 56 1513 4352
Fragments arranged by size:
4352 1513 683 56
BtsI GCAGTG_nn'
Cuts at: 3211 4120 4914 4934 3211
Size: 909 794 20 4881
Fragments arranged by size:
4881 909 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2053 2088 529
Size: 35 1489 35 5045
Fragments arranged by size:
5045 1489 35 35
DraI TTT'AAA
Cuts at: 3908 3908
Size: 6604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5611 809
Size: 507 4295 1802
Fragments arranged by size:
4295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4051 4940 6569
Size: 54 1425 50 275 18 2221 889 1629
Cuts at: 6569 8
Size: 43
Fragments arranged by size:
2221 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4051 1537
Size: 275 2239 4090
Fragments arranged by size:
4090 2239 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2248 2457 2740 2775 4534 1353
Size: 895 209 283 35 1759 3423
Fragments arranged by size:
3423 1759 895 283 209 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5191 5565 5711 387
Size: 1064 138 3602 374 146 1280
Fragments arranged by size:
3602 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2940 2952 3166 3267 4667 5264
Size: 393 1056 252 12 214 101 1400 597
Cuts at: 5264 6051 6319 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1400 1056 787 597 393 268 252
214 101 12
EcoO109I rG'GnC_Cy
Cuts at: 2135 3173 3662 2135
Size: 1038 489 5077
Fragments arranged by size:
5077 1038 489
EcoRI G'AATT_C
Cuts at: 2768 3913 4291 2768
Size: 1145 378 5081
Fragments arranged by size:
5081 1145 378
FspI TGC'GCA
Cuts at: 5110 5110
Size: 6604
HaeII r_GCGC'y
Cuts at: 1678 5751 1678
Size: 4073 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2333 2365 2549
Size: 32 135 32 509 32 220 32 184
Cuts at: 2549 2581 3059 3091 5245 5277 5319 5351 1373
Size: 32 478 32 2154 32 42 32 2626
Fragments arranged by size:
2626 2154 509 478 220 184 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3948 4194 669
Size: 988 281 1092 918 246 3079
Fragments arranged by size:
3079 1092 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6604
HpaI GTT'AAC
Cuts at: 4194 4194
Size: 6604
KpnI G_GTAC'C
Cuts at: 3929 3929
Size: 6604
MfeI C'AATT_G
Cuts at: 2655 4203 2655
Size: 1548 5056
MluI A'CGCG_T
Cuts at: 6480 6480
Size: 6604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2503 5718 5902 1305
Size: 1198 3215 184 2007
Fragments arranged by size:
3215 2007 1198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4782
Fragments arranged by size:
4782 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2337 3671 3707 3761 3773 3833 4562
Size: 648 1171 1334 36 54 12 60 729
Cuts at: 4562 4921 5080 518
Size: 359 159 2042
Fragments arranged by size:
2042 1334 1171 729 648 359 159 60
54 36 12
MspA1I CmG'CkG
Cuts at: 1606 3044 3080 3221 3782 4020 4697 5845 6090
Size: 1438 36 141 561 238 677 1148 245
Cuts at: 6090 1606
Size: 2120
Fragments arranged by size:
2120 1438 1148 677 561 245 238 141
36
NcoI C'CATG_G
Cuts at: 513 1065 3702 513
Size: 552 2637 3415
Fragments arranged by size:
3415 2637 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6604
NotI GC'GGCC_GC
Cuts at: 4051 4051
Size: 6604
NspI r_CATG'y
Cuts at: 1132 1229 2035 2212 3625 3682 3823 3962 5507
Size: 97 806 177 1413 57 141 139 1545
Cuts at: 5507 1132
Size: 2229
Fragments arranged by size:
2229 1545 1413 806 177 141 139 97
57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6604
PciI A'CATG_T
Cuts at: 2031 5503 2031
Size: 3472 3132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6604
PmeI GTTT'AAAC
Cuts at: 3908 3908
Size: 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6186 6218 4673
Size: 32 1481 32 5059
Fragments arranged by size:
5059 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 3662 2135
Size: 1527 5077
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6604
PsiI TTA'TAA
Cuts at: 2262 4174 2262
Size: 1912 4692
PstI C_TGCA'G
Cuts at: 830 3956 830
Size: 3126 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6604
PvuII CAG'CTG
Cuts at: 3044 3080 3044
Size: 36 6568
SalI G'TCGA_C
Cuts at: 1936 3946 1936
Size: 2010 4594
SanDI GG'GwC_CC
Cuts at: 3662 3662
Size: 6604
SapI GCTCTTCn'nnn_
Cuts at: 2457 2457
Size: 6604
SbfI CC_TGCA'GG
Cuts at: 3956 3956
Size: 6604
ScaI AGT'ACT
Cuts at: 4852 6564 4852
Size: 1712 4892
SexAI A'CCwGG_T
Cuts at: 3493 3493
Size: 6604
SfcI C'TryA_G
Cuts at: 826 1045 3952 5087 5768 5959 826
Size: 219 2907 1135 681 191 1471
Fragments arranged by size:
2907 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6604
SmaI CCC'GGG
Cuts at: 1872 3931 1872
Size: 2059 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4708 5609 5871 820
Size: 197 926 2765 901 262 1553
Fragments arranged by size:
2765 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3901 4395 493
Size: 3408 494 2702
Fragments arranged by size:
3408 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6604
SphI G_CATG'C
Cuts at: 1132 3682 3823 3962 1132
Size: 2550 141 139 3774
Fragments arranged by size:
3774 2550 141 139
SspI AAT'ATT
Cuts at: 5 52 2404 4528 5
Size: 47 2352 2124 2081
Fragments arranged by size:
2352 2124 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6604
StyI C'CwwG_G
Cuts at: 513 1065 2129 2166 3702 4043 513
Size: 552 1064 37 1536 341 3074
Fragments arranged by size:
3074 1536 1064 552 341 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4631 4648 4801 4986 5431 325
Size: 1255 3051 17 153 185 445 1498
Fragments arranged by size:
3051 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4850 6562 96
Size: 274 80 33 51 1443 2873 1712 138
Fragments arranged by size:
2873 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 2845 2877 6564 6596 2567
Size: 32 246 32 3687 32 2575
Fragments arranged by size:
3687 2575 246 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6604
XbaI T'CTAG_A
Cuts at: 3940 3940
Size: 6604
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6604
XmaI C'CCGG_G
Cuts at: 1870 3929 1870
Size: 2059 4545
XmnI GAAnn'nnTTC
Cuts at: 2190 4733 2190
Size: 2543 4061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4412 276
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI
SwaI XcmI