Restriction map for
pFN21ASDA0167
(Circular) (Six-base) MAPSORT of: pFN21ASDA0167.seq Check: 4651 from: 1 to: 7237
pFN21ASDA0167 7237 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3408 3408
Size: 7237
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5047 278
Size: 53 83 186 936 3511 2468
Fragments arranged by size:
3511 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4530 4580 1937
Size: 2593 50 4594
Fragments arranged by size:
4594 2593 50
Acc65I G'GTAC_C
Cuts at: 4558 4558
Size: 7237
AclI AA'CG_TT
Cuts at: 1563 5364 5737 1563
Size: 3801 373 3063
Fragments arranged by size:
3801 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2434 3425 3567 3588 5300 6684 6952 1632
Size: 802 991 142 21 1712 1384 268 1917
Fragments arranged by size:
1917 1712 1384 991 802 268 142 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7040
AflIII A'CryG_T
Cuts at: 1203 6136 7113 1203
Size: 4933 977 1327
Fragments arranged by size:
4933 1327 977
AgeI A'CCGG_T
Cuts at: 6068 6068
Size: 7237
AhdI GACnn_n'nnGTC
Cuts at: 5966 5966
Size: 7237
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2774 1184
Size: 1590 5647
AlwNI CAG_nnn'CTG
Cuts at: 2314 2435 2740 2906 4078 4980 6552 2314
Size: 121 305 166 1172 902 1572 2999
Fragments arranged by size:
2999 1572 1172 902 305 166 121
ApaI G_GGCC'C
Cuts at: 3808 3808
Size: 7237
ApaLI G'TGCA_C
Cuts at: 5294 6450 5294
Size: 1156 6081
ApoI r'AATT_y
Cuts at: 1493 3418 3781 4546 4772 4924 7033 1493
Size: 1925 363 765 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 765 363 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5791 160
Size: 872 4759 1606
Fragments arranged by size:
4759 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7237
AvaI C'yCGr_G
Cuts at: 1870 1943 2586 3220 3235 3426 4399 4562 1870
Size: 73 643 634 15 191 973 163 4545
Fragments arranged by size:
4545 973 643 634 191 163 73 15
BamHI G'GATC_C
Cuts at: 4567 4934 4567
Size: 367 6870
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2401 3839 4087 4558 6013
Size: 325 217 627 614 1438 248 471 1455
Cuts at: 6013 618
Size: 1842
Fragments arranged by size:
1842 1455 1438 627 614 471 325 248
217
BanII G_rGCy'C
Cuts at: 1391 2617 2673 2883 3282 3433 3470 3808 4257
Size: 1226 56 210 399 151 37 338 449
Cuts at: 4257 4556 1391
Size: 299 4072
Fragments arranged by size:
4072 1226 449 399 338 299 210 151
56 37
BbeI G_GCGC'C
Cuts at: 3843 4091 3843
Size: 248 6989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 2440 2680 928
Size: 971 428 113 240 5485
Fragments arranged by size:
5485 971 428 240 113
BbvCI CC'TCA_GC
Cuts at: 3262 3434 3262
Size: 172 7065
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2879 2913 5428 5462 1903
Size: 34 942 34 2515 34 3678
Fragments arranged by size:
3678 2515 942 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5129 6339 5129
Size: 1210 6027
BclI T'GATC_A
Cuts at: 1548 2190 1548
Size: 642 6595
BfrBI ATG'CAT
Cuts at: 2026 2026
Size: 7237
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 3758 4142
Size: 107 122 71 631 605 421 1665 384
Cuts at: 4142 4352 5848 136
Size: 210 1496 1525
Fragments arranged by size:
1665 1525 1496 631 605 421 384 210
122 107 71
BglII A'GATC_T
Cuts at: 7232 7232
Size: 7237
BlpI GC'TnA_GC
Cuts at: 4094 4654 4094
Size: 560 6677
Bme1580I G_kGCm'C
Cuts at: 1790 2404 3808 3887 5298 6454 1790
Size: 614 1404 79 1411 1156 2573
Fragments arranged by size:
2573 1411 1404 1156 614 79
BmgBI CAC'GTC
Cuts at: 1318 3615 4153 4264 4303 1318
Size: 2297 538 111 39 4252
Fragments arranged by size:
4252 2297 538 111 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 3354 4968
Size: 416 53 83 399 24 1727 210 1614
Cuts at: 4968 5926 442
Size: 958 1753
Fragments arranged by size:
1753 1727 1614 958 416 399 210 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4497 1056
Size: 3441 3796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2336 2477 3477 4370 5897 1239
Size: 890 207 141 1000 893 1527 2579
Fragments arranged by size:
2579 1527 1000 893 890 207 141
Bpu10I CC'TnA_GC
Cuts at: 3262 3434 3464 3262
Size: 172 30 7035
Fragments arranged by size:
7035 172 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5362 6227 6525 5362
Size: 865 298 6074
Fragments arranged by size:
6074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3453 5900 882
Size: 617 1954 2447 2219
Fragments arranged by size:
2447 2219 1954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3819 4534 5028 493
Size: 711 2615 715 494 2702
Fragments arranged by size:
2702 2615 715 711 494
BsaBI GATnn'nnATC
Cuts at: 7231 7231
Size: 7237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3840 4088 4317 4512
Size: 53 83 186 936 2307 248 229 195
Cuts at: 4512 5044 5426 6061 275
Size: 532 382 635 1451
Fragments arranged by size:
2307 1451 936 635 532 382 248 229
195 186 83 53
BsaWI w'CCGG_w
Cuts at: 2794 3414 3765 5670 6068 6342 6489 6900 2794
Size: 620 351 1905 398 274 147 411 3131
Fragments arranged by size:
3131 1905 620 411 398 351 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4031 4061 1178
Size: 30 165 30 2628 30 4354
Fragments arranged by size:
4354 2628 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2544 3529 3656 4052 4121 1186
Size: 1358 985 127 396 69 4302
Fragments arranged by size:
4302 1358 985 396 127 69
BseYI C'CCAG_C
Cuts at: 2248 2396 2424 2762 3353 3557 4114 4430 4448
Size: 148 28 338 591 204 557 316 18
Cuts at: 4448 4466 6440 6936 2248
Size: 18 1974 496 2549
Fragments arranged by size:
2549 1974 591 557 496 338 316 204
148 28 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 4160 1163
Size: 573 1179 1245 4240
Fragments arranged by size:
4240 1245 1179 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4251 4687 5448 6476 1540
Size: 275 186 2250 436 761 1028 2301
Fragments arranged by size:
2301 2250 1028 761 436 275 186
BsiHKAI G_wGCw'C
Cuts at: 2617 2883 2918 3299 4100 4257 5298 5383 6454
Size: 266 35 381 801 157 1041 85 1071
Cuts at: 6454 2617
Size: 3400
Fragments arranged by size:
3400 1071 1041 801 381 266 157 85
35
BsmI GAATG_Cn'
Cuts at: 2175 4748 4841 2175
Size: 2573 93 4571
Fragments arranged by size:
4571 2573 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2495 2495
Size: 7237
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2404 2617 2673 2883 2918 3282 3299
Size: 399 614 213 56 210 35 364 17
Cuts at: 3299 3433 3470 3808 3887 4100 4257 4556 5298
Size: 134 37 338 79 213 157 299 742
Cuts at: 5298 5383 6454 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 399 364 338 299
213 213 210 157 134 85 79 56
37 35 17
BspEI T'CCGG_A
Cuts at: 3765 6900 3765
Size: 3135 4102
BspHI T'CATG_A
Cuts at: 5126 6856 5126
Size: 1730 5507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3408 4592 844
Size: 2564 1184 3489
Fragments arranged by size:
3489 2564 1184
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 3646 4198 5124 1394
Size: 429 397 1426 552 926 3507
Fragments arranged by size:
3507 1426 926 552 429 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 3701 5732 5906 65
Size: 1183 2453 2031 174 1396
Fragments arranged by size:
2453 2031 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5881 6068 1700
Size: 110 4071 187 2869
Fragments arranged by size:
4071 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7237
BssSI C'ACGA_G
Cuts at: 2604 5297 6309 2604
Size: 2693 1012 3532
Fragments arranged by size:
3532 2693 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2408 4282 794
Size: 1614 1874 3749
Fragments arranged by size:
3749 1874 1614
BstBI TT'CG_AA
Cuts at: 2007 6041 2007
Size: 4034 3203
BstEII G'GTnAC_C
Cuts at: 1184 2713 2870 1184
Size: 1529 157 5551
Fragments arranged by size:
5551 1529 157
BstYI r'GATC_y
Cuts at: 1972 3678 4567 4934 5319 5336 6788 6874 6886
Size: 1706 889 367 385 17 1452 86 12
Cuts at: 6886 7232 1972
Size: 346 1977
Fragments arranged by size:
1977 1706 1452 889 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 4531 4531
Size: 7237
Bsu36I CC'TnA_GG
Cuts at: 2108 2108
Size: 7237
BtgI C'CryG_G
Cuts at: 513 1065 2494 3178 3498 3747 3929 513
Size: 552 1429 684 320 249 182 3821
Fragments arranged by size:
3821 1429 684 552 320 249 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2259 2268 487
Size: 683 56 1033 9 5456
Fragments arranged by size:
5456 1033 683 56 9
BtsI GCAGTG_nn'
Cuts at: 2061 2907 4753 5547 5567 2061
Size: 846 1846 794 20 3731
Fragments arranged by size:
3731 1846 846 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3774 3809 529
Size: 35 3210 35 3957
Fragments arranged by size:
3957 3210 35 35
DraI TTT'AAA
Cuts at: 4541 4541
Size: 7237
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6244 809
Size: 507 4928 1802
Fragments arranged by size:
4928 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2803 3750
Size: 54 1425 50 275 18 255 718 947
Cuts at: 3750 3926 3972 4248 4419 4437 4684 5573 7202
Size: 176 46 276 171 18 247 889 1629
Cuts at: 7202 8
Size: 43
Fragments arranged by size:
1629 1425 947 889 718 276 275 255
247 176 171 54 50 46 43 18
18
EagI C'GGCC_G
Cuts at: 1537 1812 4248 4684 1537
Size: 275 2436 436 4090
Fragments arranged by size:
4090 2436 436 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2845 3564 4033 5167 1353
Size: 1492 719 469 1134 3423
Fragments arranged by size:
3423 1492 1134 719 469
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5824 6198 6344 387
Size: 1064 138 4235 374 146 1280
Fragments arranged by size:
4235 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2615 2881 4255 2615
Size: 266 1374 5597
Fragments arranged by size:
5597 1374 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2336 2434 2477 3425 3477 3567
Size: 393 497 207 98 43 948 52 90
Cuts at: 3567 3588 4370 5300 5897 6684 6952 1239
Size: 21 782 930 597 787 268 1524
Fragments arranged by size:
1524 948 930 787 782 597 497 393
268 207 98 90 52 43 21
EcoO109I rG'GnC_Cy
Cuts at: 2925 3804 3805 4216 2925
Size: 879 1 411 5946
Fragments arranged by size:
5946 879 411 1
EcoRI G'AATT_C
Cuts at: 3781 4546 4924 3781
Size: 765 378 6094
Fragments arranged by size:
6094 765 378
FspI TGC'GCA
Cuts at: 2723 3725 5743 2723
Size: 1002 2018 4217
Fragments arranged by size:
4217 2018 1002
HaeII r_GCGC'y
Cuts at: 1678 3843 4091 4337 4491 6384 1678
Size: 2165 248 246 154 1893 2531
Fragments arranged by size:
2531 2165 1893 248 246 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2052 2084 3374 3406 4031
Size: 32 135 32 480 32 1290 32 625
Cuts at: 4031 4063 5878 5910 5952 5984 1373
Size: 32 1815 32 42 32 2626
Fragments arranged by size:
2626 1815 1290 625 480 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4581 4827 669
Size: 988 281 2643 246 3079
Fragments arranged by size:
3079 2643 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2998 748
Size: 2250 4987
HpaI GTT'AAC
Cuts at: 4827 4827
Size: 7237
KasI G'GCGC_C
Cuts at: 3839 4087 3839
Size: 248 6989
KpnI G_GTAC'C
Cuts at: 4562 4562
Size: 7237
MfeI C'AATT_G
Cuts at: 4836 4836
Size: 7237
MluI A'CGCG_T
Cuts at: 7113 7113
Size: 7237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6351 6535 1305
Size: 5046 184 2007
Fragments arranged by size:
5046 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2087 2805 3752 3928 10
Size: 54 1425 343 255 718 947 176 3319
Fragments arranged by size:
3319 1425 947 718 343 255 176 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5195 5554 5713 518
Size: 648 4029 359 159 2042
Fragments arranged by size:
4029 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2503 2783 2897 4093 4114 4126 4225 4366
Size: 897 280 114 1196 21 12 99 141
Cuts at: 4366 4653 5330 6478 6723 1606
Size: 287 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 897 677 287 280 245
141 114 99 21 12
NarI GG'CG_CC
Cuts at: 3840 4088 3840
Size: 248 6989
NcoI C'CATG_G
Cuts at: 513 1065 2494 3498 513
Size: 552 1429 1004 4252
Fragments arranged by size:
4252 1429 1004 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7237
NheI G'CTAG_C
Cuts at: 1052 4493 1052
Size: 3441 3796
NotI GC'GGCC_GC
Cuts at: 4684 4684
Size: 7237
NsiI A_TGCA'T
Cuts at: 2028 2028
Size: 7237
NspI r_CATG'y
Cuts at: 1132 1229 2030 4595 6140 1132
Size: 97 801 2565 1545 2229
Fragments arranged by size:
2565 2229 1545 801 97
PasI CC'CwG_GG
Cuts at: 1348 2921 4321 1348
Size: 1573 1400 4264
Fragments arranged by size:
4264 1573 1400
PciI A'CATG_T
Cuts at: 6136 6136
Size: 7237
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 2698 2710 4153 1108
Size: 1206 384 12 1443 4192
Fragments arranged by size:
4192 1443 1206 384 12
PfoI T'CCnGG_A
Cuts at: 2145 2691 2145
Size: 546 6691
PmeI GTTT'AAAC
Cuts at: 4541 4541
Size: 7237
PmlI CAC'GTG
Cuts at: 3819 3819
Size: 7237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5306 5338 6819 6851 5306
Size: 32 1481 32 5692
Fragments arranged by size:
5692 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2925 4216 2925
Size: 1291 5946
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7237
PsiI TTA'TAA
Cuts at: 4807 4807
Size: 7237
PspOMI G'GGCC_C
Cuts at: 3804 3804
Size: 7237
PstI C_TGCA'G
Cuts at: 830 2056 2642 2902 3372 4589 830
Size: 1226 586 260 470 1217 3478
Fragments arranged by size:
3478 1226 1217 586 470 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7237
PvuII CAG'CTG
Cuts at: 2503 2783 2897 4126 4225 4366 2503
Size: 280 114 1229 99 141 5374
Fragments arranged by size:
5374 1229 280 141 114 99
SacI G_AGCT'C
Cuts at: 2617 2883 4257 2617
Size: 266 1374 5597
Fragments arranged by size:
5597 1374 266
SalI G'TCGA_C
Cuts at: 1936 4579 1936
Size: 2643 4594
SanDI GG'GwC_CC
Cuts at: 2925 2925
Size: 7237
SbfI CC_TGCA'GG
Cuts at: 4589 4589
Size: 7237
ScaI AGT'ACT
Cuts at: 5485 7197 5485
Size: 1712 5525
SexAI A'CCwGG_T
Cuts at: 2307 2307
Size: 7237
SfcI C'TryA_G
Cuts at: 826 1045 2052 2638 2898 3368 4585 5720 6401
Size: 219 1007 586 260 470 1217 1135 681
Cuts at: 6401 6592 826
Size: 191 1471
Fragments arranged by size:
1471 1217 1135 1007 681 586 470 260
219 191
SfoI GGC'GCC
Cuts at: 3841 4089 3841
Size: 248 6989
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7237
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7237
SmaI CCC'GGG
Cuts at: 1872 3222 4401 4564 1872
Size: 1350 1179 163 4545
Fragments arranged by size:
4545 1350 1179 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5341 6242 6504 820
Size: 197 926 3398 901 262 1553
Fragments arranged by size:
3398 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4534 5028 493
Size: 4041 494 2702
Fragments arranged by size:
4041 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7237
SphI G_CATG'C
Cuts at: 1132 2030 4595 1132
Size: 898 2565 3774
Fragments arranged by size:
3774 2565 898
SspI AAT'ATT
Cuts at: 5 52 5161 5
Size: 47 5109 2081
Fragments arranged by size:
5109 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 2535 3318 1517
Size: 645 373 783 5436
Fragments arranged by size:
5436 783 645 373
StyI C'CwwG_G
Cuts at: 513 1065 2494 2541 3498 3774 4676 513
Size: 552 1429 47 957 276 902 3074
Fragments arranged by size:
3074 1429 957 902 552 276 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 3299 5264 5281 5434 5619 6064
Size: 1255 651 1068 1965 17 153 185 445
Cuts at: 6064 325
Size: 1498
Fragments arranged by size:
1965 1498 1255 1068 651 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 5483 7195
Size: 274 80 33 51 1443 360 3146 1712
Cuts at: 7195 96
Size: 138
Fragments arranged by size:
3146 1712 1443 360 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7197 7229 7197
Size: 32 7205
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7237
XbaI T'CTAG_A
Cuts at: 4573 4573
Size: 7237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3832 3832
Size: 7237
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7237
XmaI C'CCGG_G
Cuts at: 1870 3220 4399 4562 1870
Size: 1350 1179 163 4545
Fragments arranged by size:
4545 1350 1179 163
XmnI GAAnn'nnTTC
Cuts at: 2471 5366 2471
Size: 2895 4342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5045 276
Size: 53 83 186 936 3511 2468
Fragments arranged by size:
3511 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BplI BsiWI BstXI
DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PspXI PsrI RsrII SacII SapI SfiI
SrfI SwaI