Restriction map for
pFN21ASDA0163
(Circular) (Six-base) MAPSORT of: pFN21ASDA0163.seq Check: 5073 from: 1 to: 6379
pFN21ASDA0163 6379 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2921 2921
Size: 6379
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 3722 1937
Size: 478 1307 4594
Fragments arranged by size:
4594 1307 478
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 2637 2714 4442 5826 6094 1632
Size: 697 308 77 1728 1384 268 1917
Fragments arranged by size:
1917 1728 1384 697 308 268 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 2712 2831 5278 6255 1203
Size: 1509 119 2447 977 1327
Fragments arranged by size:
2447 1509 1327 977 119
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3587 1184
Size: 2403 3976
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 3386 3418 2852
Size: 32 502 32 5813
Fragments arranged by size:
5813 502 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2464 2503 3461 3511 4122 5694 2464
Size: 39 958 50 611 1572 3149
Fragments arranged by size:
3149 1572 958 611 50 39
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 3688 3914 4066 6175 1493
Size: 2195 226 152 2109 1697
Fragments arranged by size:
2195 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 3704 1870
Size: 73 594 1167 4545
Fragments arranged by size:
4545 1167 594 73
AvrII C'CTAG_G
Cuts at: 2356 2356
Size: 6379
BamHI G'GATC_C
Cuts at: 2269 3207 3709 4076 2269
Size: 938 502 367 4572
Fragments arranged by size:
4572 938 502 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2045 2141 2379 2757
Size: 325 217 627 239 19 96 238 378
Cuts at: 2757 3144 3495 3700 5155 618
Size: 387 351 205 1455 1842
Fragments arranged by size:
1842 1455 627 387 378 351 325 239
238 217 205 96 19
BanII G_rGCy'C
Cuts at: 1391 3698 1391
Size: 2307 4072
BbeI G_GCGC'C
Cuts at: 2049 3148 3499 2049
Size: 1099 351 4929
Fragments arranged by size:
4929 1099 351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2796 3552 928
Size: 971 897 756 3755
Fragments arranged by size:
3755 971 897 756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2321 2355 4570 4604 1903
Size: 34 384 34 2215 34 3678
Fragments arranged by size:
3678 2215 384 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2095 2469 3533
Size: 107 122 71 631 605 423 374 1064
Cuts at: 3533 4990 136
Size: 1457 1525
Fragments arranged by size:
1525 1457 1064 631 605 423 374 122
107 71
BglII A'GATC_T
Cuts at: 6374 6374
Size: 6379
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2369 2725 3582 4440 5596 1790
Size: 241 338 356 857 858 1156 2573
Fragments arranged by size:
2573 1156 858 857 356 338 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2446 4110 5068
Size: 416 53 83 399 24 1029 1664 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
1753 1664 1029 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3521 5039 1239
Size: 2282 1518 2579
Fragments arranged by size:
2579 2282 1518
Bpu10I CC'TnA_GC
Cuts at: 2420 2903 2420
Size: 483 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4504 5369 5667 4504
Size: 865 298 5216
Fragments arranged by size:
5216 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3676 4170 493
Size: 711 2472 494 2702
Fragments arranged by size:
2702 2472 711 494
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 3145 3496 4186
Size: 53 83 186 936 513 1099 351 690
Cuts at: 4186 4568 5203 275
Size: 382 635 1451
Fragments arranged by size:
1451 1099 936 690 635 513 382 351
186 83 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2536 2566 3365 3395 3466
Size: 30 165 30 1133 30 799 30 71
Cuts at: 3466 3496 1178
Size: 30 4061
Fragments arranged by size:
4061 1133 799 165 71 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2307 3079 1186
Size: 1121 772 4486
Fragments arranged by size:
4486 1121 772
BseYI C'CCAG_C
Cuts at: 2181 2628 2724 3610 5582 6078 2181
Size: 447 96 886 1972 496 2482
Fragments arranged by size:
2482 1972 886 496 447 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2893 1163
Size: 573 1157 4649
Fragments arranged by size:
4649 1157 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3829 4590 5618 1540
Size: 275 186 1201 627 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 627 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 3608 4440 4525 5596 2076
Size: 1532 832 85 1071 2859
Fragments arranged by size:
2859 1532 1071 832 85
BsmI GAATG_Cn'
Cuts at: 2639 3890 3983 2639
Size: 1251 93 5035
Fragments arranged by size:
5035 1251 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2076 2369 2725 3582 3608 3698
Size: 399 241 45 293 356 857 26 90
Cuts at: 3698 4440 4525 5596 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 857 742 399 356 293 241
90 85 45 26
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2462 2921 3734 844
Size: 1618 459 813 3489
Fragments arranged by size:
3489 1618 813 459
BsrBI CCG'CTC
Cuts at: 1394 1823 2424 2542 2613 3600 4266 1394
Size: 429 601 118 71 987 666 3507
Fragments arranged by size:
3507 987 666 601 429 118 71
BsrDI GCAATG_nn'
Cuts at: 65 1248 4874 5048 65
Size: 1183 3626 174 1396
Fragments arranged by size:
3626 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3129 5023 5210 1700
Size: 110 1319 1894 187 2869
Fragments arranged by size:
2869 1894 1319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 2069 3575 4439 5451 2069
Size: 1506 864 1012 2997
Fragments arranged by size:
2997 1506 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3511 794
Size: 2717 3662
BstBI TT'CG_AA
Cuts at: 2007 5183 2007
Size: 3176 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2269 3207 3709 4076 4461 4478 5930 6016
Size: 297 938 502 367 385 17 1452 86
Cuts at: 6016 6028 6374 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 938 502 385 367 346 297
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2883 2883
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2022 2273 2917 3051 513
Size: 552 957 251 644 134 3841
Fragments arranged by size:
3841 957 644 552 251 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 3107 3516 3623 3895 4689 4709 2177
Size: 930 409 107 272 794 20 3847
Fragments arranged by size:
3847 930 794 409 272 107 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DraIII CAC_nnn'GTG
Cuts at: 2320 2320
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2397 2602
Size: 54 1425 50 275 18 266 301 205
Cuts at: 2602 2691 3054 3199 3346 3637 3826 4715 6344
Size: 89 363 145 147 291 189 889 1629
Cuts at: 6344 8
Size: 43
Fragments arranged by size:
1629 1425 889 363 301 291 275 266
205 189 147 145 89 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 3826 1537
Size: 275 1387 627 4090
Fragments arranged by size:
4090 1387 627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2656 3122 4309 1353
Size: 713 590 466 1187 3423
Fragments arranged by size:
3423 1187 713 590 466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3354 4966 5340 5486 387
Size: 1064 138 1765 1612 374 146 1280
Fragments arranged by size:
1765 1612 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2637 2714 3521 4442 5039 5826
Size: 393 697 308 77 807 921 597 787
Cuts at: 5826 6094 1239
Size: 268 1524
Fragments arranged by size:
1524 921 807 787 697 597 393 308
268 77
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 2519
Size: 6379
EcoO109I rG'GnC_Cy
Cuts at: 2961 2989 3525 2961
Size: 28 536 5815
Fragments arranged by size:
5815 536 28
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 2049 2464 3148 3499 3570 5526 1678
Size: 371 415 684 351 71 1956 2531
Fragments arranged by size:
2531 1956 684 415 371 351 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5020 5052 5094 5126 1373
Size: 32 135 32 3448 32 42 32 2626
Fragments arranged by size:
3448 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2839 3723 3969 669
Size: 988 281 901 884 246 3079
Fragments arranged by size:
3079 988 901 884 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KasI G'GCGC_C
Cuts at: 2045 3144 3495 2045
Size: 1099 351 4929
Fragments arranged by size:
4929 1099 351
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2534 5493 5677 1305
Size: 1229 2959 184 2007
Fragments arranged by size:
2959 2007 1229 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3056 10
Size: 54 1425 343 1224 3333
Fragments arranged by size:
3333 1425 1224 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2812 4337 4696 4855 518
Size: 648 1646 1525 359 159 2042
Fragments arranged by size:
2042 1646 1525 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2092 2139 2275 2403 2775 3060 3081
Size: 437 49 47 136 128 372 285 21
Cuts at: 3081 3142 3259 3614 3795 4472 5620 5865 1606
Size: 61 117 355 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 437 372 355 285 245
181 136 128 117 61 49 47 21
NarI GG'CG_CC
Cuts at: 2046 3145 3496 2046
Size: 1099 351 4929
Fragments arranged by size:
4929 1099 351
NcoI C'CATG_G
Cuts at: 513 1065 2022 2917 3051 513
Size: 552 957 895 134 3841
Fragments arranged by size:
3841 957 895 552 134
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2835 3737 5282 1132
Size: 97 1606 902 1545 2229
Fragments arranged by size:
2229 1606 1545 902 97
PasI CC'CwG_GG
Cuts at: 1348 2117 2965 3461 1348
Size: 769 848 496 4266
Fragments arranged by size:
4266 848 769 496
PciI A'CATG_T
Cuts at: 2831 5278 2831
Size: 2447 3932
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3461 3635 1108
Size: 2353 174 3852
Fragments arranged by size:
3852 2353 174
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2989 2989
Size: 6379
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PstI C_TGCA'G
Cuts at: 830 3418 3619 3731 830
Size: 2588 201 112 3478
Fragments arranged by size:
3478 2588 201 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2403 2775 3081 3614 2403
Size: 372 306 533 5168
Fragments arranged by size:
5168 533 372 306
SacII CC_GC'GG
Cuts at: 2276 2276
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SapI GCTCTTCn'nnn_
Cuts at: 2066 2656 3122 2066
Size: 590 466 5323
Fragments arranged by size:
5323 590 466
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 3414 3615 3727 4862 5543 5734 826
Size: 219 2369 201 112 1135 681 191 1471
Fragments arranged by size:
2369 1471 1135 681 219 201 191 112
SfoI GGC'GCC
Cuts at: 2047 3146 3497 2047
Size: 1099 351 4929
Fragments arranged by size:
4929 1099 351
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4483 5384 5646 820
Size: 197 926 2540 901 262 1553
Fragments arranged by size:
2540 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3676 4170 493
Size: 3183 494 2702
Fragments arranged by size:
3183 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 3380 1517
Size: 1863 4516
StyI C'CwwG_G
Cuts at: 513 1065 2022 2031 2356 2616 2917 3051 3818
Size: 552 957 9 325 260 301 134 767
Cuts at: 3818 513
Size: 3074
Fragments arranged by size:
3074 957 767 552 325 301 260 134
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4406 4423 4576 4761 5206 325
Size: 1255 2826 17 153 185 445 1498
Fragments arranged by size:
2826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4625 6337 96
Size: 274 80 33 51 1443 2648 1712 138
Fragments arranged by size:
2648 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3466 3498 6339 6371 3466
Size: 32 2841 32 3474
Fragments arranged by size:
3474 2841 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2465 2465
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI
SwaI