(Circular) (Six-base) MAPSORT of: pFN21ASDA0163.seq Check: 5073 from: 1 to: 6379 pFN21ASDA0163 6379 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2921 2921 Size: 6379 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2415 3722 1937 Size: 478 1307 4594 Fragments arranged by size: 4594 1307 478 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 2637 2714 4442 5826 6094 1632 Size: 697 308 77 1728 1384 268 1917 Fragments arranged by size: 1917 1728 1384 697 308 268 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 2712 2831 5278 6255 1203 Size: 1509 119 2447 977 1327 Fragments arranged by size: 2447 1509 1327 977 119 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3587 1184 Size: 2403 3976 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2852 2884 3386 3418 2852 Size: 32 502 32 5813 Fragments arranged by size: 5813 502 32 32 AlwNI CAG_nnn'CTG Cuts at: 2464 2503 3461 3511 4122 5694 2464 Size: 39 958 50 611 1572 3149 Fragments arranged by size: 3149 1572 958 611 50 39 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 3688 3914 4066 6175 1493 Size: 2195 226 152 2109 1697 Fragments arranged by size: 2195 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2537 3704 1870 Size: 73 594 1167 4545 Fragments arranged by size: 4545 1167 594 73 AvrII C'CTAG_G Cuts at: 2356 2356 Size: 6379 BamHI G'GATC_C Cuts at: 2269 3207 3709 4076 2269 Size: 938 502 367 4572 Fragments arranged by size: 4572 938 502 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2045 2141 2379 2757 Size: 325 217 627 239 19 96 238 378 Cuts at: 2757 3144 3495 3700 5155 618 Size: 387 351 205 1455 1842 Fragments arranged by size: 1842 1455 627 387 378 351 325 239 238 217 205 96 19 BanII G_rGCy'C Cuts at: 1391 3698 1391 Size: 2307 4072 BbeI G_GCGC'C Cuts at: 2049 3148 3499 2049 Size: 1099 351 4929 Fragments arranged by size: 4929 1099 351 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2796 3552 928 Size: 971 897 756 3755 Fragments arranged by size: 3755 971 897 756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2321 2355 4570 4604 1903 Size: 34 384 34 2215 34 3678 Fragments arranged by size: 3678 2215 384 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4271 5481 4271 Size: 1210 5169 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2095 2469 3533 Size: 107 122 71 631 605 423 374 1064 Cuts at: 3533 4990 136 Size: 1457 1525 Fragments arranged by size: 1525 1457 1064 631 605 423 374 122 107 71 BglII A'GATC_T Cuts at: 6374 6374 Size: 6379 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 2031 2369 2725 3582 4440 5596 1790 Size: 241 338 356 857 858 1156 2573 Fragments arranged by size: 2573 1156 858 857 356 338 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2446 4110 5068 Size: 416 53 83 399 24 1029 1664 958 Cuts at: 5068 442 Size: 1753 Fragments arranged by size: 1753 1664 1029 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3521 5039 1239 Size: 2282 1518 2579 Fragments arranged by size: 2579 2282 1518 Bpu10I CC'TnA_GC Cuts at: 2420 2903 2420 Size: 483 5896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4504 5369 5667 4504 Size: 865 298 5216 Fragments arranged by size: 5216 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5042 882 Size: 617 3543 2219 Fragments arranged by size: 3543 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3676 4170 493 Size: 711 2472 494 2702 Fragments arranged by size: 2702 2472 711 494 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 3145 3496 4186 Size: 53 83 186 936 513 1099 351 690 Cuts at: 4186 4568 5203 275 Size: 382 635 1451 Fragments arranged by size: 1451 1099 936 690 635 513 382 351 186 83 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2536 2566 3365 3395 3466 Size: 30 165 30 1133 30 799 30 71 Cuts at: 3466 3496 1178 Size: 30 4061 Fragments arranged by size: 4061 1133 799 165 71 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2307 3079 1186 Size: 1121 772 4486 Fragments arranged by size: 4486 1121 772 BseYI C'CCAG_C Cuts at: 2181 2628 2724 3610 5582 6078 2181 Size: 447 96 886 1972 496 2482 Fragments arranged by size: 2482 1972 886 496 447 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2893 1163 Size: 573 1157 4649 Fragments arranged by size: 4649 1157 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3829 4590 5618 1540 Size: 275 186 1201 627 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 627 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 3608 4440 4525 5596 2076 Size: 1532 832 85 1071 2859 Fragments arranged by size: 2859 1532 1071 832 85 BsmI GAATG_Cn' Cuts at: 2639 3890 3983 2639 Size: 1251 93 5035 Fragments arranged by size: 5035 1251 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2076 2369 2725 3582 3608 3698 Size: 399 241 45 293 356 857 26 90 Cuts at: 3698 4440 4525 5596 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 857 742 399 356 293 241 90 85 45 26 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2462 2921 3734 844 Size: 1618 459 813 3489 Fragments arranged by size: 3489 1618 813 459 BsrBI CCG'CTC Cuts at: 1394 1823 2424 2542 2613 3600 4266 1394 Size: 429 601 118 71 987 666 3507 Fragments arranged by size: 3507 987 666 601 429 118 71 BsrDI GCAATG_nn' Cuts at: 65 1248 4874 5048 65 Size: 1183 3626 174 1396 Fragments arranged by size: 3626 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3129 5023 5210 1700 Size: 110 1319 1894 187 2869 Fragments arranged by size: 2869 1894 1319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6379 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 2069 3575 4439 5451 2069 Size: 1506 864 1012 2997 Fragments arranged by size: 2997 1506 1012 864 BstAPI GCAn_nnn'nTGC Cuts at: 794 3511 794 Size: 2717 3662 BstBI TT'CG_AA Cuts at: 2007 5183 2007 Size: 3176 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2269 3207 3709 4076 4461 4478 5930 6016 Size: 297 938 502 367 385 17 1452 86 Cuts at: 6016 6028 6374 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 938 502 385 367 346 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2883 2883 Size: 6379 BtgI C'CryG_G Cuts at: 513 1065 2022 2273 2917 3051 513 Size: 552 957 251 644 134 3841 Fragments arranged by size: 3841 957 644 552 251 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2177 3107 3516 3623 3895 4689 4709 2177 Size: 930 409 107 272 794 20 3847 Fragments arranged by size: 3847 930 794 409 272 107 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DraIII CAC_nnn'GTG Cuts at: 2320 2320 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2397 2602 Size: 54 1425 50 275 18 266 301 205 Cuts at: 2602 2691 3054 3199 3346 3637 3826 4715 6344 Size: 89 363 145 147 291 189 889 1629 Cuts at: 6344 8 Size: 43 Fragments arranged by size: 1629 1425 889 363 301 291 275 266 205 189 147 145 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 3826 1537 Size: 275 1387 627 4090 Fragments arranged by size: 4090 1387 627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2656 3122 4309 1353 Size: 713 590 466 1187 3423 Fragments arranged by size: 3423 1187 713 590 466 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3354 4966 5340 5486 387 Size: 1064 138 1765 1612 374 146 1280 Fragments arranged by size: 1765 1612 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2329 2637 2714 3521 4442 5039 5826 Size: 393 697 308 77 807 921 597 787 Cuts at: 5826 6094 1239 Size: 268 1524 Fragments arranged by size: 1524 921 807 787 697 597 393 308 268 77 EcoNI CCTnn'n_nnAGG Cuts at: 2519 2519 Size: 6379 EcoO109I rG'GnC_Cy Cuts at: 2961 2989 3525 2961 Size: 28 536 5815 Fragments arranged by size: 5815 536 28 EcoRI G'AATT_C Cuts at: 3688 4066 3688 Size: 378 6001 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 2049 2464 3148 3499 3570 5526 1678 Size: 371 415 684 351 71 1956 2531 Fragments arranged by size: 2531 1956 684 415 371 351 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5020 5052 5094 5126 1373 Size: 32 135 32 3448 32 42 32 2626 Fragments arranged by size: 3448 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2839 3723 3969 669 Size: 988 281 901 884 246 3079 Fragments arranged by size: 3079 988 901 884 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KasI G'GCGC_C Cuts at: 2045 3144 3495 2045 Size: 1099 351 4929 Fragments arranged by size: 4929 1099 351 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2534 5493 5677 1305 Size: 1229 2959 184 2007 Fragments arranged by size: 2959 2007 1229 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3056 10 Size: 54 1425 343 1224 3333 Fragments arranged by size: 3333 1425 1224 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2812 4337 4696 4855 518 Size: 648 1646 1525 359 159 2042 Fragments arranged by size: 2042 1646 1525 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2092 2139 2275 2403 2775 3060 3081 Size: 437 49 47 136 128 372 285 21 Cuts at: 3081 3142 3259 3614 3795 4472 5620 5865 1606 Size: 61 117 355 181 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 437 372 355 285 245 181 136 128 117 61 49 47 21 NarI GG'CG_CC Cuts at: 2046 3145 3496 2046 Size: 1099 351 4929 Fragments arranged by size: 4929 1099 351 NcoI C'CATG_G Cuts at: 513 1065 2022 2917 3051 513 Size: 552 957 895 134 3841 Fragments arranged by size: 3841 957 895 552 134 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 2835 3737 5282 1132 Size: 97 1606 902 1545 2229 Fragments arranged by size: 2229 1606 1545 902 97 PasI CC'CwG_GG Cuts at: 1348 2117 2965 3461 1348 Size: 769 848 496 4266 Fragments arranged by size: 4266 848 769 496 PciI A'CATG_T Cuts at: 2831 5278 2831 Size: 2447 3932 PflMI CCAn_nnn'nTGG Cuts at: 1108 3461 3635 1108 Size: 2353 174 3852 Fragments arranged by size: 3852 2353 174 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2989 2989 Size: 6379 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PstI C_TGCA'G Cuts at: 830 3418 3619 3731 830 Size: 2588 201 112 3478 Fragments arranged by size: 3478 2588 201 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 2403 2775 3081 3614 2403 Size: 372 306 533 5168 Fragments arranged by size: 5168 533 372 306 SacII CC_GC'GG Cuts at: 2276 2276 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SapI GCTCTTCn'nnn_ Cuts at: 2066 2656 3122 2066 Size: 590 466 5323 Fragments arranged by size: 5323 590 466 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SfcI C'TryA_G Cuts at: 826 1045 3414 3615 3727 4862 5543 5734 826 Size: 219 2369 201 112 1135 681 191 1471 Fragments arranged by size: 2369 1471 1135 681 219 201 191 112 SfoI GGC'GCC Cuts at: 2047 3146 3497 2047 Size: 1099 351 4929 Fragments arranged by size: 4929 1099 351 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4483 5384 5646 820 Size: 197 926 2540 901 262 1553 Fragments arranged by size: 2540 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3676 4170 493 Size: 3183 494 2702 Fragments arranged by size: 3183 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 3380 1517 Size: 1863 4516 StyI C'CwwG_G Cuts at: 513 1065 2022 2031 2356 2616 2917 3051 3818 Size: 552 957 9 325 260 301 134 767 Cuts at: 3818 513 Size: 3074 Fragments arranged by size: 3074 957 767 552 325 301 260 134 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4406 4423 4576 4761 5206 325 Size: 1255 2826 17 153 185 445 1498 Fragments arranged by size: 2826 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4625 6337 96 Size: 274 80 33 51 1443 2648 1712 138 Fragments arranged by size: 2648 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3466 3498 6339 6371 3466 Size: 32 2841 32 3474 Fragments arranged by size: 3474 2841 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2465 2465 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 4508 4508 Size: 6379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI