Restriction map for
pFN21ASDA0158
(Circular) (Six-base) MAPSORT of: pFN21ASDA0158.seq Check: 242 from: 1 to: 5812
pFN21ASDA0158 5812 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 2406 3133 2406
Size: 727 5085
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 2627 2864 3875 5259 5527 1632
Size: 977 18 237 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1011 977 268 237 18
AflII C'TTAA_G
Cuts at: 820 1017 2501 820
Size: 197 1484 4131
Fragments arranged by size:
4131 1484 197
AflIII A'CryG_T
Cuts at: 1203 3102 4711 5688 1203
Size: 1899 1609 977 1327
Fragments arranged by size:
1899 1609 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 3137 1870
Size: 73 1149 45 4545
Fragments arranged by size:
4545 1149 73 45
BamHI G'GATC_C
Cuts at: 2744 3142 3509 2744
Size: 398 367 5047
Fragments arranged by size:
5047 398 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2406 3096 3133 4588 618
Size: 325 217 627 619 690 37 1455 1842
Fragments arranged by size:
1842 1455 690 627 619 325 217 37
BanII G_rGCy'C
Cuts at: 1391 3009 3091 3131 1391
Size: 1618 82 40 4072
Fragments arranged by size:
4072 1618 82 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4841
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2982 5807 2982
Size: 2825 2987
BlpI GC'TnA_GC
Cuts at: 3003 3229 3003
Size: 226 5586
Bme1580I G_kGCm'C
Cuts at: 1790 3099 3873 5029 1790
Size: 1309 774 1156 2573
Fragments arranged by size:
2573 1309 1156 774
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2724 1056
Size: 1668 4144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2807
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2250 2867 4472 1239
Size: 1011 617 1605 2579
Fragments arranged by size:
2579 1605 1011 617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2918 3937 4802 5100 2699
Size: 219 1019 865 298 3411
Fragments arranged by size:
3411 1019 865 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3103 3109 3603 493
Size: 711 1899 6 494 2702
Fragments arranged by size:
2702 1899 711 494 6
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3619 4001 4636 275
Size: 53 83 186 936 2086 382 635 1451
Fragments arranged by size:
2086 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5812
BseYI C'CCAG_C
Cuts at: 5015 5511 5015
Size: 496 5316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2832 3009 3873 3958 5029 2832
Size: 177 864 85 1071 3615
Fragments arranged by size:
3615 1071 864 177 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2832 3009 3091 3099 3131 3873 3958
Size: 399 1042 177 82 8 32 742 85
Cuts at: 3958 5029 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1042 742 399 177 85 82
32 8
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 3167 844
Size: 1574 749 3489
Fragments arranged by size:
3489 1574 749
BsrBI CCG'CTC
Cuts at: 1394 1823 2424 2602 3699 1394
Size: 429 601 178 1097 3507
Fragments arranged by size:
3507 1097 601 429 178
BsrDI GCAATG_nn'
Cuts at: 65 1248 2562 4307 4481 65
Size: 1183 1314 1745 174 1396
Fragments arranged by size:
1745 1396 1314 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5812
BstBI TT'CG_AA
Cuts at: 2007 4616 2007
Size: 2609 3203
BstEII G'GTnAC_C
Cuts at: 1184 2890 1184
Size: 1706 4106
BstYI r'GATC_y
Cuts at: 1972 2744 2878 2982 3142 3509 3894 3911 5363
Size: 772 134 104 160 367 385 17 1452
Cuts at: 5363 5449 5461 5807 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 772 385 367 346 160 134
104 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3093 487
Size: 683 56 1867 3206
Fragments arranged by size:
3206 1867 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 2695 3116 2695
Size: 421 5391
DraIII CAC_nnn'GTG
Cuts at: 3103 3103
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777
Size: 54 1425 50 275 18 1429 889 1629
Cuts at: 5777 8
Size: 43
Fragments arranged by size:
1629 1429 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 3742 1353
Size: 930 1459 3423
Fragments arranged by size:
3423 1459 930
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2999 4399 4773 4919 387
Size: 1064 138 1410 1400 374 146 1280
Fragments arranged by size:
1410 1400 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3007 3007
Size: 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2609 2627 2864 2867 3875 4472
Size: 393 618 359 18 237 3 1008 597
Cuts at: 4472 5259 5527 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 618 597 393 359 268
237 18 3
EcoO109I rG'GnC_Cy
Cuts at: 2899 2899
Size: 5812
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2657 2689 4453 4485 4527
Size: 32 135 32 1085 32 1764 32 42
Cuts at: 4527 4559 1373
Size: 32 2626
Fragments arranged by size:
2626 1764 1085 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3156 3402 669
Size: 988 281 1218 246 3079
Fragments arranged by size:
3079 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KpnI G_GTAC'C
Cuts at: 2410 3137 2410
Size: 727 5085
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2056 2722 4926 5110 1305
Size: 751 666 2204 184 2007
Fragments arranged by size:
2204 2007 751 666 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2870 3770 4129 4288 518
Size: 648 975 729 900 359 159 2042
Fragments arranged by size:
2042 975 900 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3228 3905 5053 5298 1606
Size: 1622 677 1148 245 2120
Fragments arranged by size:
2120 1622 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5260
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 2720 1052
Size: 1668 4144
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2875 3019 3170 4715 1132
Size: 97 1646 144 151 1545 2229
Fragments arranged by size:
2229 1646 1545 151 144 97
PasI CC'CwG_GG
Cuts at: 1348 2799 1348
Size: 1451 4361
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PfoI T'CCnGG_A
Cuts at: 2883 2883
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PmlI CAC'GTG
Cuts at: 3103 3103
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2899 2899
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 2051 3382 2051
Size: 1331 4481
PstI C_TGCA'G
Cuts at: 830 2357 3164 830
Size: 1527 807 3478
Fragments arranged by size:
3478 1527 807
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
SacI G_AGCT'C
Cuts at: 3009 3009
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SapI GCTCTTCn'nnn_
Cuts at: 2283 2283
Size: 5812
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2353 3160 4295 4976 5167 826
Size: 219 1308 807 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 807 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2714 2933 3916 4817 5079
Size: 197 926 558 213 219 983 901 262
Cuts at: 5079 820
Size: 1553
Fragments arranged by size:
1553 983 926 901 558 262 219 213
197
SnaBI TAC'GTA
Cuts at: 493 3109 3603 493
Size: 2616 494 2702
Fragments arranged by size:
2702 2616 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3019 3170 1132
Size: 1887 151 3774
Fragments arranged by size:
3774 1887 151
SspI AAT'ATT
Cuts at: 5 52 2551 3736 5
Size: 47 2499 1185 2081
Fragments arranged by size:
2499 2081 1185 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 3251 513
Size: 552 2186 3074
Fragments arranged by size:
3074 2186 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 3839 3856 4009 4194 4639 325
Size: 1255 1302 957 17 153 185 445 1498
Fragments arranged by size:
1498 1302 1255 957 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4058 5770 96
Size: 274 80 33 51 1443 2081 1712 138
Fragments arranged by size:
2081 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI