Restriction map for
pFN21ASDA0154
(Circular) (Six-base) MAPSORT of: pFN21ASDA0154.seq Check: 6893 from: 1 to: 6799
pFN21ASDA0154 6799 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3186 3949 3186
Size: 763 6036
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4609 278
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4142 1937
Size: 2205 4594
Acc65I G'GTAC_C
Cuts at: 4120 4120
Size: 6799
AclI AA'CG_TT
Cuts at: 1563 4926 5299 1563
Size: 3363 373 3063
Fragments arranged by size:
3363 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3196 4001 4862 6246 6514 1632
Size: 725 839 805 861 1384 268 1917
Fragments arranged by size:
1917 1384 861 839 805 725 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6602
AflIII A'CryG_T
Cuts at: 1203 2571 5698 6675 1203
Size: 1368 3127 977 1327
Fragments arranged by size:
3127 1368 1327 977
AgeI A'CCGG_T
Cuts at: 5630 5630
Size: 6799
AhdI GACnn_n'nnGTC
Cuts at: 2239 2562 5528 2239
Size: 323 2966 3510
Fragments arranged by size:
3510 2966 323
AleI CACnn'nnGTG
Cuts at: 1166 4043 1166
Size: 2877 3922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3695 3869 1184
Size: 2511 174 4114
Fragments arranged by size:
4114 2511 174
AlwNI CAG_nnn'CTG
Cuts at: 2218 3326 3425 3616 4542 6114 2218
Size: 1108 99 191 926 1572 2903
Fragments arranged by size:
2903 1572 1108 926 191 99
ApaI G_GGCC'C
Cuts at: 3063 3594 3063
Size: 531 6268
ApaLI G'TGCA_C
Cuts at: 4856 6012 4856
Size: 1156 5643
ApoI r'AATT_y
Cuts at: 1493 4108 4334 4486 6595 1493
Size: 2615 226 152 2109 1697
Fragments arranged by size:
2615 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5353 160
Size: 872 4321 1606
Fragments arranged by size:
4321 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
AvaI C'yCGr_G
Cuts at: 1870 1943 2784 3511 4124 1870
Size: 73 841 727 613 4545
Fragments arranged by size:
4545 841 727 613 73
BamHI G'GATC_C
Cuts at: 2544 4129 4496 2544
Size: 1585 367 4847
Fragments arranged by size:
4847 1585 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3174 3242 3429 4120 5575
Size: 325 217 627 1387 68 187 691 1455
Cuts at: 5575 618
Size: 1842
Fragments arranged by size:
1842 1455 1387 691 627 325 217 187
68
BanII G_rGCy'C
Cuts at: 1391 3063 3365 3417 3594 3660 4118 1391
Size: 1672 302 52 177 66 458 4072
Fragments arranged by size:
4072 1672 458 302 177 66 52
BbeI G_GCGC'C
Cuts at: 3246 3246
Size: 6799
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2261 2538 928
Size: 971 362 277 5189
Fragments arranged by size:
5189 971 362 277
BbvCI CC'TCA_GC
Cuts at: 3271 3271
Size: 6799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2884 2918 4990 5024 1903
Size: 34 947 34 2072 34 3678
Fragments arranged by size:
3678 2072 947 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4691 5901 4691
Size: 1210 5589
BclI T'GATC_A
Cuts at: 1548 2122 2680 1548
Size: 574 558 5667
Fragments arranged by size:
5667 574 558
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5410 136
Size: 107 122 71 631 605 3738 1525
Fragments arranged by size:
3738 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6794 6794
Size: 6799
BlpI GC'TnA_GC
Cuts at: 4034 4216 4034
Size: 182 6617
Bme1580I G_kGCm'C
Cuts at: 1790 3063 3432 3594 3684 3712 3870 4860 6016
Size: 1273 369 162 90 28 158 990 1156
Cuts at: 6016 1790
Size: 2573
Fragments arranged by size:
2573 1273 1156 990 369 162 158 90
28
BmgBI CAC'GTC
Cuts at: 1318 3793 1318
Size: 2475 4324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 3348 4530
Size: 416 53 83 399 24 690 1241 1182
Cuts at: 4530 5488 442
Size: 958 1753
Fragments arranged by size:
1753 1241 1182 958 690 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2195 3167 3248 3402 5459 1239
Size: 830 126 972 81 154 2057 2579
Fragments arranged by size:
2579 2057 972 830 154 126 81
Bpu10I CC'TnA_GC
Cuts at: 3271 3654 3271
Size: 383 6416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3268 4924 5789 6087 3268
Size: 1656 865 298 3980
Fragments arranged by size:
3980 1656 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5462 882
Size: 617 3963 2219
Fragments arranged by size:
3963 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3679 4096 4590 493
Size: 711 2475 417 494 2702
Fragments arranged by size:
2702 2475 711 494 417
BsaBI GATnn'nnATC
Cuts at: 6793 6793
Size: 6799
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 3243 4606 4988
Size: 53 83 186 936 789 921 1363 382
Cuts at: 4988 5623 275
Size: 635 1451
Fragments arranged by size:
1451 1363 936 921 789 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5232 5630 5904 6051 6462 5232
Size: 398 274 147 411 5569
Fragments arranged by size:
5569 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 3068 3098 1178
Size: 30 165 30 1362 30 273 30 4879
Fragments arranged by size:
4879 1362 273 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 2962 3022 3113 3246 3271 3355 1186
Size: 1267 509 60 91 133 25 84 4630
Fragments arranged by size:
4630 1267 509 133 91 84 60 25
BseYI C'CCAG_C
Cuts at: 2953 3062 3127 3158 3319 3334 3391 3559 6002
Size: 109 65 31 161 15 57 168 2443
Cuts at: 6002 6498 2953
Size: 496 3254
Fragments arranged by size:
3254 2443 496 168 161 109 65 57
31 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 2552 2902 3446 1163
Size: 573 639 177 350 544 4516
Fragments arranged by size:
4516 639 573 544 350 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4249 5010 6038 1540
Size: 275 186 2248 761 1028 2301
Fragments arranged by size:
2301 2248 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3660 4040 4860 4945 6016 3660
Size: 380 820 85 1071 4443
Fragments arranged by size:
4443 1071 820 380 85
BsmI GAATG_Cn'
Cuts at: 4310 4403 4310
Size: 93 6706
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3063 3365 3417 3432 3594 3660 3684
Size: 399 1273 302 52 15 162 66 24
Cuts at: 3684 3712 3870 4040 4118 4860 4945 6016 1391
Size: 28 158 170 78 742 85 1071 2174
Fragments arranged by size:
2174 1273 1071 742 399 302 170 162
158 85 78 66 52 28 24 15
BspEI T'CCGG_A
Cuts at: 6462 6462
Size: 6799
BspHI T'CATG_A
Cuts at: 4688 6418 4688
Size: 1730 5069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2812 3186 3949 4154 844
Size: 1655 313 374 763 205 3489
Fragments arranged by size:
3489 1655 763 374 313 205
BsrBI CCG'CTC
Cuts at: 1394 1823 3030 3604 4686 1394
Size: 429 1207 574 1082 3507
Fragments arranged by size:
3507 1207 1082 574 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5294 5468 65
Size: 1183 4046 174 1396
Fragments arranged by size:
4046 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5443 5630 1700
Size: 110 3633 187 2869
Fragments arranged by size:
3633 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2277 96
Size: 2181 4618
BssHII G'CGCG_C
Cuts at: 1925 3456 1925
Size: 1531 5268
BssSI C'ACGA_G
Cuts at: 4859 5871 4859
Size: 1012 5787
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6799
BstBI TT'CG_AA
Cuts at: 2007 5603 2007
Size: 3596 3203
BstEII G'GTnAC_C
Cuts at: 1184 2554 1184
Size: 1370 5429
BstXI CCAn_nnnn'nTGG
Cuts at: 2199 2199
Size: 6799
BstYI r'GATC_y
Cuts at: 1972 2181 2544 4129 4496 4881 4898 6350 6436
Size: 209 363 1585 367 385 17 1452 86
Cuts at: 6436 6448 6794 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1585 1452 385 367 363 346 209
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2974 3010 2974
Size: 36 6763
BtgI C'CryG_G
Cuts at: 513 1065 2022 2289 2520 3020 3433 3585 513
Size: 552 957 267 231 500 413 152 3727
Fragments arranged by size:
3727 957 552 500 413 267 231 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6060
Fragments arranged by size:
6060 683 56
BtsI GCAGTG_nn'
Cuts at: 3302 3712 3870 4315 5109 5129 3302
Size: 410 158 445 794 20 4972
Fragments arranged by size:
4972 794 445 410 158 20
ClaI AT'CG_AT
Cuts at: 1554 3692 1554
Size: 2138 4661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2358 2393 529
Size: 35 1794 35 4935
Fragments arranged by size:
4935 1794 35 35
DraI TTT'AAA
Cuts at: 2713 4103 2713
Size: 1390 5409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5806 809
Size: 507 4490 1802
Fragments arranged by size:
4490 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3666 3969 4246
Size: 54 1425 50 275 18 1836 303 277
Cuts at: 4246 5135 6764 8
Size: 889 1629 43
Fragments arranged by size:
1836 1629 1425 889 303 277 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4246 1537
Size: 275 2434 4090
Fragments arranged by size:
4090 2434 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2647 3489 3611 3758 4729 1353
Size: 980 314 842 122 147 971 3423
Fragments arranged by size:
3423 980 971 842 314 147 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2048 2716 5386 5760 5906
Size: 1064 138 453 6 668 2670 374 146
Cuts at: 5906 387
Size: 1280
Fragments arranged by size:
2670 1280 1064 668 453 374 146 138
6
EcoICRI GAG'CTC
Cuts at: 3658 3658
Size: 6799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2195 2357 3167 3196 3248 3402
Size: 393 437 126 162 810 29 52 154
Cuts at: 3402 4001 4862 5459 6246 6514 1239
Size: 599 861 597 787 268 1524
Fragments arranged by size:
1524 861 810 787 599 597 437 393
268 162 154 126 52 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2495 2495
Size: 6799
EcoO109I rG'GnC_Cy
Cuts at: 2215 3014 3059 3567 3590 3650 2215
Size: 799 45 508 23 60 5364
Fragments arranged by size:
5364 799 508 60 45 23
EcoRI G'AATT_C
Cuts at: 4108 4486 4108
Size: 378 6421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4030 4030
Size: 6799
FspI TGC'GCA
Cuts at: 5305 5305
Size: 6799
HaeII r_GCGC'y
Cuts at: 1678 2210 3246 5946 1678
Size: 532 1036 2700 2531
Fragments arranged by size:
2700 2531 1036 532
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2383 2415 2429
Size: 32 135 32 653 32 126 32 14
Cuts at: 2429 2461 2548 2580 2765 2797 5440 5472 5514
Size: 32 87 32 185 32 2643 32 42
Cuts at: 5514 5546 1373
Size: 32 2626
Fragments arranged by size:
2643 2626 653 185 135 126 87 42
32 32 32 32 32 32 32 32
32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 4143 4389 669
Size: 988 281 649 1556 246 3079
Fragments arranged by size:
3079 1556 988 649 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 748
Size: 1702 5097
HpaI GTT'AAC
Cuts at: 2587 4389 2587
Size: 1802 4997
KasI G'GCGC_C
Cuts at: 3242 3242
Size: 6799
KpnI G_GTAC'C
Cuts at: 4124 4124
Size: 6799
MfeI C'AATT_G
Cuts at: 4398 4398
Size: 6799
MluI A'CGCG_T
Cuts at: 6675 6675
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 2188 3222 3273 5913 6097 1305
Size: 801 82 1034 51 2640 184 2007
Fragments arranged by size:
2640 2007 1034 801 184 82 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3668 3971 10
Size: 54 1425 343 1836 303 2838
Fragments arranged by size:
2838 1836 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2197 3471 3677 4043 4757 5116 5275
Size: 648 1031 1274 206 366 714 359 159
Cuts at: 5275 518
Size: 2042
Fragments arranged by size:
2042 1274 1031 714 648 366 359 206
159
MspA1I CmG'CkG
Cuts at: 1606 2283 3158 3233 4033 4215 4892 6040 6285
Size: 677 875 75 800 182 677 1148 245
Cuts at: 6285 1606
Size: 2120
Fragments arranged by size:
2120 1148 875 800 677 677 245 182
75
NarI GG'CG_CC
Cuts at: 3243 3243
Size: 6799
NcoI C'CATG_G
Cuts at: 513 1065 2022 2289 2520 3433 513
Size: 552 957 267 231 913 3879
Fragments arranged by size:
3879 957 913 552 267 231
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6799
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6799
NotI GC'GGCC_GC
Cuts at: 4246 4246
Size: 6799
NspI r_CATG'y
Cuts at: 1132 1229 2335 4157 5702 1132
Size: 97 1106 1822 1545 2229
Fragments arranged by size:
2229 1822 1545 1106 97
PasI CC'CwG_GG
Cuts at: 1348 3055 3425 3631 4021 1348
Size: 1707 370 206 390 4126
Fragments arranged by size:
4126 1707 390 370 206
PciI A'CATG_T
Cuts at: 5698 5698
Size: 6799
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3439 3902 1108
Size: 2331 463 4005
Fragments arranged by size:
4005 2331 463
PmeI GTTT'AAAC
Cuts at: 4103 4103
Size: 6799
PmlI CAC'GTG
Cuts at: 3679 3679
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4868 4900 6381 6413 4868
Size: 32 1481 32 5254
Fragments arranged by size:
5254 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3014 3014
Size: 6799
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6799
PsiI TTA'TAA
Cuts at: 4369 4369
Size: 6799
PspOMI G'GGCC_C
Cuts at: 3059 3590 3059
Size: 531 6268
PstI C_TGCA'G
Cuts at: 830 2496 3858 3865 3894 3946 4151 830
Size: 1666 1362 7 29 52 205 3478
Fragments arranged by size:
3478 1666 1362 205 52 29 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6799
PvuII CAG'CTG
Cuts at: 2283 3158 4033 2283
Size: 875 875 5049
Fragments arranged by size:
5049 875 875
SacI G_AGCT'C
Cuts at: 3660 3660
Size: 6799
SalI G'TCGA_C
Cuts at: 1936 4141 1936
Size: 2205 4594
SapI GCTCTTCn'nnn_
Cuts at: 3611 3611
Size: 6799
SbfI CC_TGCA'GG
Cuts at: 2496 4151 2496
Size: 1655 5144
ScaI AGT'ACT
Cuts at: 5047 6759 5047
Size: 1712 5087
SfcI C'TryA_G
Cuts at: 826 1045 2492 3854 3861 3890 3942 4147 5282
Size: 219 1447 1362 7 29 52 205 1135
Cuts at: 5282 5963 6154 826
Size: 681 191 1471
Fragments arranged by size:
1471 1447 1362 1135 681 219 205 191
52 29 7
SfoI GGC'GCC
Cuts at: 3244 3244
Size: 6799
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6799
SmaI CCC'GGG
Cuts at: 1872 2786 4126 1872
Size: 914 1340 4545
Fragments arranged by size:
4545 1340 914
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3283 4903 5804 6066 820
Size: 197 926 1340 1620 901 262 1553
Fragments arranged by size:
1620 1553 1340 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4096 4590 493
Size: 3603 494 2702
Fragments arranged by size:
3603 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6799
SphI G_CATG'C
Cuts at: 1132 4157 1132
Size: 3025 3774
SspI AAT'ATT
Cuts at: 5 52 4723 5
Size: 47 4671 2081
Fragments arranged by size:
4671 2081 47
StuI AGG'CCT
Cuts at: 1517 3008 1517
Size: 1491 5308
StyI C'CwwG_G
Cuts at: 513 1065 2022 2259 2289 2520 2890 3396 3433
Size: 552 957 237 30 231 370 506 37
Cuts at: 3433 3765 4238 513
Size: 332 473 3074
Fragments arranged by size:
3074 957 552 506 473 370 332 237
231 37 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 4826 4843 4996 5181 5626 325
Size: 1255 2388 858 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 858 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 5045 6757
Size: 274 80 33 51 1443 300 2768 1712
Cuts at: 6757 96
Size: 138
Fragments arranged by size:
2768 1712 1443 300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3606 3638 6759 6791 3606
Size: 32 3121 32 3614
Fragments arranged by size:
3614 3121 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6799
XbaI T'CTAG_A
Cuts at: 3490 4135 3490
Size: 645 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6799
XmaI C'CCGG_G
Cuts at: 1870 2784 4124 1870
Size: 914 1340 4545
Fragments arranged by size:
4545 1340 914
XmnI GAAnn'nnTTC
Cuts at: 4928 4928
Size: 6799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4607 276
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI