Restriction map for
pFN21ASDA0149
(Circular) (Six-base) MAPSORT of: pFN21ASDA0149.seq Check: 960 from: 1 to: 7219
pFN21ASDA0149 7219 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2744 3695 2744
Size: 951 6268
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5029 278
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4562 1937
Size: 2625 4594
Acc65I G'GTAC_C
Cuts at: 3056 3806 4540 3056
Size: 750 734 5735
Fragments arranged by size:
5735 750 734
AclI AA'CG_TT
Cuts at: 1563 5346 5719 1563
Size: 3783 373 3063
Fragments arranged by size:
3783 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4467 5282 6666 6934 1632
Size: 2835 815 1384 268 1917
Fragments arranged by size:
2835 1917 1384 815 268
AfeI AGC'GCT
Cuts at: 3005 3005
Size: 7219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7022
AflIII A'CryG_T
Cuts at: 1203 6118 7095 1203
Size: 4915 977 1327
Fragments arranged by size:
4915 1327 977
AgeI A'CCGG_T
Cuts at: 4002 6050 4002
Size: 2048 5171
AhdI GACnn_n'nnGTC
Cuts at: 5948 5948
Size: 7219
AleI CACnn'nnGTG
Cuts at: 1166 2109 2814 1166
Size: 943 705 5571
Fragments arranged by size:
5571 943 705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3323 1184
Size: 2139 5080
AlwNI CAG_nnn'CTG
Cuts at: 2990 3269 3995 4034 4228 4406 4962 6534 2990
Size: 279 726 39 194 178 556 1572 3675
Fragments arranged by size:
3675 1572 726 556 279 194 178 39
ApaI G_GGCC'C
Cuts at: 2275 2312 3030 3159 3364 4082 2275
Size: 37 718 129 205 718 5412
Fragments arranged by size:
5412 718 718 205 129 37
ApaLI G'TGCA_C
Cuts at: 2731 5276 6432 2731
Size: 2545 1156 3518
Fragments arranged by size:
3518 2545 1156
ApoI r'AATT_y
Cuts at: 1493 4528 4754 4906 7015 1493
Size: 3035 226 152 2109 1697
Fragments arranged by size:
3035 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5773 160
Size: 872 4741 1606
Fragments arranged by size:
4741 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2293 3771 3882 3953 3980 4075
Size: 73 121 229 1478 111 71 27 95
Cuts at: 4075 4544 1870
Size: 469 4545
Fragments arranged by size:
4545 1478 469 229 121 111 95 73
71 27
BamHI G'GATC_C
Cuts at: 4121 4429 4549 4916 4121
Size: 308 120 367 6424
Fragments arranged by size:
6424 367 308 120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2552 2583 2921 3056 3806
Size: 325 217 627 765 31 338 135 750
Cuts at: 3806 4370 4540 5995 618
Size: 564 170 1455 1842
Fragments arranged by size:
1842 1455 765 750 627 564 338 325
217 170 135 31
BanII G_rGCy'C
Cuts at: 1391 2275 2312 2404 3030 3159 3364 3458 3849
Size: 884 37 92 626 129 205 94 391
Cuts at: 3849 4076 4082 4283 4393 4538 1391
Size: 227 6 201 110 145 4072
Fragments arranged by size:
4072 884 626 391 227 205 201 145
129 110 94 92 37 6
BbeI G_GCGC'C
Cuts at: 2556 2587 2556
Size: 31 7188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3045 3075 4138 928
Size: 971 1146 30 1063 4009
Fragments arranged by size:
4009 1146 1063 971 30
BbvCI CC'TCA_GC
Cuts at: 3479 3479
Size: 7219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 5410 5444 1903
Size: 34 2144 34 1295 34 3678
Fragments arranged by size:
3678 2144 1295 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5111 6321 5111
Size: 1210 6009
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7219
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2658 2745 2784
Size: 107 122 71 631 605 986 87 39
Cuts at: 2784 2919 3264 3572 3698 3874 5830 136
Size: 135 345 308 126 176 1956 1525
Fragments arranged by size:
1956 1525 986 631 605 345 308 176
135 126 122 107 87 71 39
BglII A'GATC_T
Cuts at: 7214 7214
Size: 7219
BlpI GC'TnA_GC
Cuts at: 2133 4035 4636 2133
Size: 1902 601 4716
Fragments arranged by size:
4716 1902 601
Bme1580I G_kGCm'C
Cuts at: 1790 2275 2312 2735 2954 3030 3159 3364 4082
Size: 485 37 423 219 76 129 205 718
Cuts at: 4082 5280 6436 1790
Size: 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 718 485 423 219 205
129 76 37
BmgBI CAC'GTC
Cuts at: 1318 2107 1318
Size: 789 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 2890 3160
Size: 416 53 83 399 24 897 576 270
Cuts at: 3160 4950 5908 442
Size: 1790 958 1753
Fragments arranged by size:
1790 1753 958 897 576 416 399 270
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2860 3577 3748 4420 2650
Size: 210 717 171 672 5449
Fragments arranged by size:
5449 717 672 210 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2832 3630 3745 3835 4480 5879 1239
Size: 937 656 798 115 90 645 1399 2579
Fragments arranged by size:
2579 1399 937 798 656 645 115 90
Bpu10I CC'TnA_GC
Cuts at: 3479 3838 4209 3479
Size: 359 371 6489
Fragments arranged by size:
6489 371 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3362 5344 6209 6507 3362
Size: 1982 865 298 4074
Fragments arranged by size:
4074 1982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3796 5882 882
Size: 617 2297 2086 2219
Fragments arranged by size:
2297 2219 2086 617
BsaAI yAC'GTr
Cuts at: 493 1204 2496 4042 4516 5010 493
Size: 711 1292 1546 474 494 2702
Fragments arranged by size:
2702 1546 1292 711 494 474
BsaBI GATnn'nnATC
Cuts at: 7213 7213
Size: 7219
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 2553 2584 3729
Size: 53 83 186 936 672 348 31 1145
Cuts at: 3729 5026 5408 6043 275
Size: 1297 382 635 1451
Fragments arranged by size:
1451 1297 1145 936 672 635 382 348
186 83 53 31
BsaWI w'CCGG_w
Cuts at: 4002 5652 6050 6324 6471 6882 4002
Size: 1650 398 274 147 411 4339
Fragments arranged by size:
4339 1650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 3393 3423 1178
Size: 30 165 30 864 30 1096 30 4974
Fragments arranged by size:
4974 1096 864 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7219
BseYI C'CCAG_C
Cuts at: 2029 2394 3022 3158 3178 3357 3570 4301 4360
Size: 365 628 136 20 179 213 731 59
Cuts at: 4360 6422 6918 2029
Size: 2062 496 2330
Fragments arranged by size:
2330 2062 731 628 496 365 213 179
136 59 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 1163
Size: 573 1083 5563
Fragments arranged by size:
5563 1083 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2392 2457 2641 2657 4669 5430
Size: 275 186 391 65 184 16 2012 761
Cuts at: 5430 6458 1540
Size: 1028 2301
Fragments arranged by size:
2301 2012 1028 761 391 275 186 184
65 16
BsiHKAI G_wGCw'C
Cuts at: 2735 2846 3849 4041 4076 5280 5365 6436 2735
Size: 111 1003 192 35 1204 85 1071 3518
Fragments arranged by size:
3518 1204 1071 1003 192 111 85 35
BsmI GAATG_Cn'
Cuts at: 2180 2681 3224 4730 4823 2180
Size: 501 543 1506 93 4576
Fragments arranged by size:
4576 1506 543 501 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2312 2404 2735 2846 2954 3030
Size: 399 485 37 92 331 111 108 76
Cuts at: 3030 3159 3364 3458 3849 4041 4076 4082 4283
Size: 129 205 94 391 192 35 6 201
Cuts at: 4283 4393 4538 5280 5365 6436 1391
Size: 110 145 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 391 331 205
201 192 145 129 111 110 108 94
92 85 76 37 35 6
BspEI T'CCGG_A
Cuts at: 6882 6882
Size: 7219
BspHI T'CATG_A
Cuts at: 5108 6838 5108
Size: 1730 5489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2744 3092 3418 3695 4574 844
Size: 1900 348 326 277 879 3489
Fragments arranged by size:
3489 1900 879 348 326 277
BsrBI CCG'CTC
Cuts at: 1394 1823 3859 4140 5106 1394
Size: 429 2036 281 966 3507
Fragments arranged by size:
3507 2036 966 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 5714 5888 65
Size: 1183 4466 174 1396
Fragments arranged by size:
4466 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2456 2652 3143 3182 3566 4002 5863
Size: 110 646 196 491 39 384 436 1861
Cuts at: 5863 6050 1700
Size: 187 2869
Fragments arranged by size:
2869 1861 646 491 436 384 196 187
110 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7219
BssHII G'CGCG_C
Cuts at: 1925 2531 2759 1925
Size: 606 228 6385
Fragments arranged by size:
6385 606 228
BssSI C'ACGA_G
Cuts at: 5279 6291 5279
Size: 1012 6207
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7219
BstBI TT'CG_AA
Cuts at: 2007 6023 2007
Size: 4016 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7219
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 7219
BstYI r'GATC_y
Cuts at: 1972 4121 4429 4549 4916 5301 5318 6770 6856
Size: 2149 308 120 367 385 17 1452 86
Cuts at: 6856 6868 7214 1972
Size: 12 346 1977
Fragments arranged by size:
2149 1977 1452 385 367 346 308 120
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3723 3903 3723
Size: 180 7039
BtgI C'CryG_G
Cuts at: 513 1065 2022 2364 2434 2523 2547 2611 2752
Size: 552 957 342 70 89 24 64 141
Cuts at: 2752 2791 3190 4117 513
Size: 39 399 927 3615
Fragments arranged by size:
3615 957 927 552 399 342 141 89
70 64 39 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6480
Fragments arranged by size:
6480 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 2275 2467 2693 2807 2954 2956 3145 3282
Size: 128 192 226 114 147 2 189 137
Cuts at: 3282 4735 5529 5549 2147
Size: 1453 794 20 3817
Fragments arranged by size:
3817 1453 794 226 192 189 147 137
128 114 20 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7184
DraI TTT'AAA
Cuts at: 4523 4523
Size: 7219
DraIII CAC_nnn'GTG
Cuts at: 2797 2797
Size: 7219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2077 3527 6226 809
Size: 507 761 1450 2699 1802
Fragments arranged by size:
2699 1802 1450 761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2347 2638
Size: 54 1425 50 275 18 285 232 291
Cuts at: 2638 2654 2818 2992 3782 3997 4273 4666 5555
Size: 16 164 174 790 215 276 393 889
Cuts at: 5555 7184 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 790 393 291 285 276
275 232 215 174 164 54 50 43
18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2638 2654 4666 1537
Size: 275 826 16 2012 4090
Fragments arranged by size:
4090 2012 826 275 16
EarI CTCTTCn'nnn_
Cuts at: 1353 3329 3915 4443 5149 1353
Size: 1976 586 528 706 3423
Fragments arranged by size:
3423 1976 706 586 528
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4139 4196 5806 6180 6326 387
Size: 1064 138 2550 57 1610 374 146 1280
Fragments arranged by size:
2550 1610 1280 1064 374 146 138 57
EcoICRI GAG'CTC
Cuts at: 3847 4074 3847
Size: 227 6992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2832 3630 3745 3835 4467 4480
Size: 393 544 656 798 115 90 632 13
Cuts at: 4480 5282 5879 6666 6934 1239
Size: 802 597 787 268 1524
Fragments arranged by size:
1524 802 798 787 656 632 597 544
393 268 115 90 13
EcoO109I rG'GnC_Cy
Cuts at: 2271 2308 2309 2669 2892 3026 3105 3155 3252
Size: 37 1 360 223 134 79 50 97
Cuts at: 3252 3361 3529 3899 4079 4130 4225 4322 2271
Size: 109 168 370 180 51 95 97 5168
Fragments arranged by size:
5168 370 360 223 180 168 134 109
97 97 95 79 51 50 37 1
EcoRI G'AATT_C
Cuts at: 4528 4906 4528
Size: 378 6841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4322 4322
Size: 7219
FspI TGC'GCA
Cuts at: 2149 2806 5725 2149
Size: 657 2919 3643
Fragments arranged by size:
3643 2919 657
FspAI rTGC'GCAy
Cuts at: 2806 2806
Size: 7219
HaeII r_GCGC'y
Cuts at: 1678 2443 2556 2587 2718 3007 6366 1678
Size: 765 113 31 131 289 3359 2531
Fragments arranged by size:
3359 2531 765 289 131 113 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3062 3094 3360 3392 3575
Size: 32 135 32 1490 32 266 32 183
Cuts at: 3575 3590 3607 3622 5860 5892 5934 5966 1373
Size: 15 17 15 2238 32 42 32 2626
Fragments arranged by size:
2626 2238 1490 266 183 135 42 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4563 4809 669
Size: 988 281 2625 246 3079
Fragments arranged by size:
3079 2625 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7219
HpaI GTT'AAC
Cuts at: 4809 4809
Size: 7219
KasI G'GCGC_C
Cuts at: 2552 2583 2552
Size: 31 7188
KpnI G_GTAC'C
Cuts at: 3060 3810 4544 3060
Size: 750 734 5735
Fragments arranged by size:
5735 750 734
MfeI C'AATT_G
Cuts at: 4818 4818
Size: 7219
MluI A'CGCG_T
Cuts at: 7095 7095
Size: 7219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6333 6517 1305
Size: 5028 184 2007
Fragments arranged by size:
5028 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2994 3999 10
Size: 54 1425 343 285 877 1005 3230
Fragments arranged by size:
3230 1425 1005 877 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 2814 5177 5536 5695 518
Size: 648 943 705 2363 359 159 2042
Fragments arranged by size:
2363 2042 943 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2289 2394 2406 2525 2589 2601 2643
Size: 441 242 105 12 119 64 12 42
Cuts at: 2643 2709 2754 2814 2823 2862 3162 4119 4635
Size: 66 45 60 9 39 300 957 516
Cuts at: 4635 5312 6460 6705 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 957 677 516 441 300 245
242 119 105 66 64 60 45 42
39 12 12 9
NaeI GCC'GGC
Cuts at: 2654 3145 3568 2654
Size: 491 423 6305
Fragments arranged by size:
6305 491 423
NarI GG'CG_CC
Cuts at: 2553 2584 2553
Size: 31 7188
NcoI C'CATG_G
Cuts at: 513 1065 2022 3190 513
Size: 552 957 1168 4542
Fragments arranged by size:
4542 1168 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7219
NgoMIV G'CCGG_C
Cuts at: 2652 3143 3566 2652
Size: 491 423 6305
Fragments arranged by size:
6305 491 423
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7219
NotI GC'GGCC_GC
Cuts at: 4666 4666
Size: 7219
NspI r_CATG'y
Cuts at: 1132 1229 4102 4577 6122 1132
Size: 97 2873 475 1545 2229
Fragments arranged by size:
2873 2229 1545 475 97
PasI CC'CwG_GG
Cuts at: 1348 3498 1348
Size: 2150 5069
PciI A'CATG_T
Cuts at: 6118 6118
Size: 7219
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2990 3269 4301 4406 1108
Size: 1882 279 1032 105 3921
Fragments arranged by size:
3921 1882 1032 279 105
PfoI T'CCnGG_A
Cuts at: 3413 4065 3413
Size: 652 6567
PmeI GTTT'AAAC
Cuts at: 4523 4523
Size: 7219
PmlI CAC'GTG
Cuts at: 2496 4042 2496
Size: 1546 5673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5288 5320 6801 6833 5288
Size: 32 1481 32 5674
Fragments arranged by size:
5674 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2669 2892 3105 3252 3529 3899 2669
Size: 223 213 147 277 370 5989
Fragments arranged by size:
5989 370 277 223 213 147
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7219
PsiI TTA'TAA
Cuts at: 4789 4789
Size: 7219
PspOMI G'GGCC_C
Cuts at: 2271 2308 3026 3155 3360 4078 2271
Size: 37 718 129 205 718 5412
Fragments arranged by size:
5412 718 718 205 129 37
PstI C_TGCA'G
Cuts at: 830 2142 2282 2594 2714 3140 3429 3706 4571
Size: 1312 140 312 120 426 289 277 865
Cuts at: 4571 830
Size: 3478
Fragments arranged by size:
3478 1312 865 426 312 289 277 140
120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7219
PvuII CAG'CTG
Cuts at: 2814 2862 3162 2814
Size: 48 300 6871
Fragments arranged by size:
6871 300 48
RsrII CG'GwC_CG
Cuts at: 4351 4351
Size: 7219
SacI G_AGCT'C
Cuts at: 3849 4076 3849
Size: 227 6992
SacII CC_GC'GG
Cuts at: 2526 2755 4120 2526
Size: 229 1365 5625
Fragments arranged by size:
5625 1365 229
SalI G'TCGA_C
Cuts at: 1936 4561 1936
Size: 2625 4594
SanDI GG'GwC_CC
Cuts at: 2669 2892 2669
Size: 223 6996
SapI GCTCTTCn'nnn_
Cuts at: 3329 3329
Size: 7219
SbfI CC_TGCA'GG
Cuts at: 4571 4571
Size: 7219
ScaI AGT'ACT
Cuts at: 2303 5467 7179 2303
Size: 3164 1712 2343
Fragments arranged by size:
3164 2343 1712
SfcI C'TryA_G
Cuts at: 826 1045 2138 2278 2590 2710 3136 3425 3702
Size: 219 1093 140 312 120 426 289 277
Cuts at: 3702 4567 5702 6383 6574 826
Size: 865 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 865 681 426 312 289
277 219 191 140 120
SfoI GGC'GCC
Cuts at: 2554 2585 2554
Size: 31 7188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
SgrAI Cr'CCGG_yG
Cuts at: 1700 4002 1700
Size: 2302 4917
SmaI CCC'GGG
Cuts at: 1872 2295 3884 3982 4546 1872
Size: 423 1589 98 564 4545
Fragments arranged by size:
4545 1589 564 423 98
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3377 5323 6224 6486 820
Size: 197 926 1434 1946 901 262 1553
Fragments arranged by size:
1946 1553 1434 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4516 5010 493
Size: 4023 494 2702
Fragments arranged by size:
4023 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7219
SphI G_CATG'C
Cuts at: 1132 4577 1132
Size: 3445 3774
SrfI GCCC'GGGC
Cuts at: 2295 2295
Size: 7219
SspI AAT'ATT
Cuts at: 5 52 5143 5
Size: 47 5091 2081
Fragments arranged by size:
5091 2081 47
StuI AGG'CCT
Cuts at: 1517 2975 1517
Size: 1458 5761
StyI C'CwwG_G
Cuts at: 513 1065 2022 3190 4188 4658 513
Size: 552 957 1168 998 470 3074
Fragments arranged by size:
3074 1168 998 957 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3490 3778 5246 5263 5416 5601 6046
Size: 1255 1910 288 1468 17 153 185 445
Cuts at: 6046 325
Size: 1498
Fragments arranged by size:
1910 1498 1468 1255 445 288 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 5465 7177
Size: 274 80 33 51 1443 324 3164 1712
Cuts at: 7177 96
Size: 138
Fragments arranged by size:
3164 1712 1443 324 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 3393 3425 7179 7211 2267
Size: 32 1094 32 3754 32 2275
Fragments arranged by size:
3754 2275 1094 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3131 1316
Size: 1815 5404
XbaI T'CTAG_A
Cuts at: 4555 4555
Size: 7219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3245 3266 4324 3245
Size: 21 1058 6140
Fragments arranged by size:
6140 1058 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7219
XmaI C'CCGG_G
Cuts at: 1870 2293 3882 3980 4544 1870
Size: 423 1589 98 564 4545
Fragments arranged by size:
4545 1589 564 423 98
XmnI GAAnn'nnTTC
Cuts at: 5348 5348
Size: 7219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5027 276
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoNI EcoRV FseI NruI NsiI PacI PspXI PsrI
SexAI SfiI SwaI