Restriction map for
pFN21ASDA0146
(Circular) (Six-base) MAPSORT of: pFN21ASDA0146.seq Check: 868 from: 1 to: 7474
pFN21ASDA0146 7474 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4527 4527
Size: 7474
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5284 278
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4817 1937
Size: 2880 4594
Acc65I G'GTAC_C
Cuts at: 3953 4795 3953
Size: 842 6632
AclI AA'CG_TT
Cuts at: 1563 5601 5974 1563
Size: 4038 373 3063
Fragments arranged by size:
4038 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2244 4022 4161 4232 5537 6921 7189
Size: 549 63 1778 139 71 1305 1384 268
Cuts at: 7189 1632
Size: 1917
Fragments arranged by size:
1917 1778 1384 1305 549 268 139 71
63
AflII C'TTAA_G
Cuts at: 820 1017 3922 820
Size: 197 2905 4372
Fragments arranged by size:
4372 2905 197
AflIII A'CryG_T
Cuts at: 1203 4416 6373 7350 1203
Size: 3213 1957 977 1327
Fragments arranged by size:
3213 1957 1327 977
AgeI A'CCGG_T
Cuts at: 6305 6305
Size: 7474
AhdI GACnn_n'nnGTC
Cuts at: 6203 6203
Size: 7474
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7474
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3666 3698 4221 4253 3666
Size: 32 523 32 6887
Fragments arranged by size:
6887 523 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3461 5217 6789 3461
Size: 1756 1572 4146
Fragments arranged by size:
4146 1756 1572
ApaLI G'TGCA_C
Cuts at: 3611 5531 6687 3611
Size: 1920 1156 4398
Fragments arranged by size:
4398 1920 1156
ApoI r'AATT_y
Cuts at: 1493 2447 2562 2773 2928 4783 5009 5161 7270
Size: 954 115 211 155 1855 226 152 2109
Cuts at: 7270 1493
Size: 1697
Fragments arranged by size:
2109 1855 1697 954 226 211 155 152
115
AseI AT'TA_AT
Cuts at: 160 1032 6028 160
Size: 872 4996 1606
Fragments arranged by size:
4996 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 3564 4485 4799 1870
Size: 73 100 1521 921 314 4545
Fragments arranged by size:
4545 1521 921 314 100 73
BamHI G'GATC_C
Cuts at: 4804 5171 4804
Size: 367 7107
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3953 4460 4515 4795 6250
Size: 325 217 627 2166 507 55 280 1455
Cuts at: 6250 618
Size: 1842
Fragments arranged by size:
2166 1842 1455 627 507 325 280 217
55
BanII G_rGCy'C
Cuts at: 1391 2051 2330 4139 4793 1391
Size: 660 279 1809 654 4072
Fragments arranged by size:
4072 1809 660 654 279
BbeI G_GCGC'C
Cuts at: 4464 4464
Size: 7474
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 3885 4458 4519 928
Size: 971 338 1648 573 61 3883
Fragments arranged by size:
3883 1648 971 573 338 61
BbvCI CC'TCA_GC
Cuts at: 4601 4601
Size: 7474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4373 4407 5665 5699 1903
Size: 34 2436 34 1258 34 3678
Fragments arranged by size:
3678 2436 1258 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5366 6576 5366
Size: 1210 6264
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7474
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6085 136
Size: 107 122 71 631 605 4413 1525
Fragments arranged by size:
4413 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2843 4032 7469 2843
Size: 1189 3437 2848
Fragments arranged by size:
3437 2848 1189
BlpI GC'TnA_GC
Cuts at: 4891 4891
Size: 7474
Bme1580I G_kGCm'C
Cuts at: 1790 3366 3615 5535 6691 1790
Size: 1576 249 1920 1156 2573
Fragments arranged by size:
2573 1920 1576 1156 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2413 5205 6163
Size: 416 53 83 399 24 996 2792 958
Cuts at: 6163 442
Size: 1753
Fragments arranged by size:
2792 1753 996 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7474
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 3429 3950 2144
Size: 1285 521 5668
Fragments arranged by size:
5668 1285 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2314 2751 3051 3483 3801 3891 3920 4040
Size: 1075 437 300 432 318 90 29 120
Cuts at: 4040 4169 6134 1239
Size: 129 1965 2579
Fragments arranged by size:
2579 1965 1075 437 432 318 300 129
120 90 29
Bpu10I CC'TnA_GC
Cuts at: 2171 3538 4601 2171
Size: 1367 1063 5044
Fragments arranged by size:
5044 1367 1063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4493 4528 5599 6464 6762 4493
Size: 35 1071 865 298 5205
Fragments arranged by size:
5205 1071 865 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 2711 4074 4276 6137 882
Size: 617 633 579 1363 202 1861 2219
Fragments arranged by size:
2219 1861 1363 633 617 579 202
BsaAI yAC'GTr
Cuts at: 493 1204 2650 4771 5265 493
Size: 711 1446 2121 494 2702
Fragments arranged by size:
2702 2121 1446 711 494
BsaBI GATnn'nnATC
Cuts at: 7468 7468
Size: 7474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4461 5281 5663 6298
Size: 53 83 186 936 2928 820 382 635
Cuts at: 6298 275
Size: 1451
Fragments arranged by size:
2928 1451 936 820 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3102 3229 5907 6305 6579 6726 7137 3102
Size: 127 2678 398 274 147 411 3439
Fragments arranged by size:
3439 2678 411 398 274 147 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2317 2347 3382 3412 3450
Size: 30 165 30 914 30 1035 30 38
Cuts at: 3450 3456 3480 3486 1178
Size: 6 24 6 5166
Fragments arranged by size:
5166 1035 914 165 38 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2995 3804 4088 1186
Size: 1809 809 284 4572
Fragments arranged by size:
4572 1809 809 284
BseYI C'CCAG_C
Cuts at: 3500 3553 3748 4132 4160 6677 7173 3500
Size: 53 195 384 28 2517 496 3801
Fragments arranged by size:
3801 2517 496 384 195 53 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2672 4576 4764 1163
Size: 573 936 1904 188 3873
Fragments arranged by size:
3873 1904 936 573 188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4924 5685 6713 1540
Size: 275 186 2923 761 1028 2301
Fragments arranged by size:
2923 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2330 3615 4391 5535 5620 6691 2330
Size: 1285 776 1144 85 1071 3113
Fragments arranged by size:
3113 1285 1144 1071 776 85
BsmI GAATG_Cn'
Cuts at: 2084 4985 5078 2084
Size: 2901 93 4480
Fragments arranged by size:
4480 2901 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2330 3366 3615 4139 4391 4793
Size: 399 261 279 1036 249 524 252 402
Cuts at: 4793 5535 5620 6691 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1036 742 524 402 399 279
261 252 249 85
BspEI T'CCGG_A
Cuts at: 3102 3229 7137 3102
Size: 127 3908 3439
Fragments arranged by size:
3908 3439 127
BspHI T'CATG_A
Cuts at: 5363 7093 5363
Size: 1730 5744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2101 2114 4513 4527 4829 844
Size: 1257 13 2399 14 302 3489
Fragments arranged by size:
3489 2399 1257 302 14 13
BsrBI CCG'CTC
Cuts at: 1394 1823 4542 5361 1394
Size: 429 2719 819 3507
Fragments arranged by size:
3507 2719 819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5969 6143 65
Size: 1183 4721 174 1396
Fragments arranged by size:
4721 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4613 6118 6305 1700
Size: 110 2803 1505 187 2869
Fragments arranged by size:
2869 2803 1505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7474
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7474
BssSI C'ACGA_G
Cuts at: 3448 5534 6546 3448
Size: 2086 1012 4376
Fragments arranged by size:
4376 2086 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2666 794
Size: 1872 5602
BstBI TT'CG_AA
Cuts at: 2007 6278 2007
Size: 4271 3203
BstEII G'GTnAC_C
Cuts at: 1184 2988 1184
Size: 1804 5670
BstYI r'GATC_y
Cuts at: 1972 2695 2843 3105 4032 4804 5171 5556 5573
Size: 723 148 262 927 772 367 385 17
Cuts at: 5573 7025 7111 7123 7469 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 927 772 723 385 367 346
262 148 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3077 3404 3077
Size: 327 7147
BtgI C'CryG_G
Cuts at: 513 1065 2029 3358 3975 513
Size: 552 964 1329 617 4012
Fragments arranged by size:
4012 1329 964 617 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6735
Fragments arranged by size:
6735 683 56
BtsI GCAGTG_nn'
Cuts at: 2105 4360 4389 4559 4990 5784 5804 2105
Size: 2255 29 170 431 794 20 3775
Fragments arranged by size:
3775 2255 794 431 170 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7474
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7439
DraI TTT'AAA
Cuts at: 4778 4778
Size: 7474
DraIII CAC_nnn'GTG
Cuts at: 3741 3741
Size: 7474
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6481 809
Size: 507 5165 1802
Fragments arranged by size:
5165 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3452 3772 4921
Size: 54 1425 50 275 18 1622 320 1149
Cuts at: 4921 5810 7439 8
Size: 889 1629 43
Fragments arranged by size:
1629 1622 1425 1149 889 320 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4921 1537
Size: 275 3109 4090
Fragments arranged by size:
4090 3109 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2113 2220 3056 3501 3786 3998 4187 4634
Size: 760 107 836 445 285 212 189 447
Cuts at: 4634 5404 1353
Size: 770 3423
Fragments arranged by size:
3423 836 770 760 447 445 285 212
189 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6061 6435 6581 387
Size: 1064 138 4472 374 146 1280
Fragments arranged by size:
4472 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2328 2328
Size: 7474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2244 2314 2751 3051 3483 3801
Size: 393 549 63 70 437 300 432 318
Cuts at: 3801 3891 3920 4022 4040 4161 4169 4232 5537
Size: 90 29 102 18 121 8 63 1305
Cuts at: 5537 6134 6921 7189 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1305 787 597 549 437 432 393
318 300 268 121 102 90 70 63
63 29 18 8
EcoNI CCTnn'n_nnAGG
Cuts at: 4652 4652
Size: 7474
EcoO109I rG'GnC_Cy
Cuts at: 4752 4752
Size: 7474
EcoRI G'AATT_C
Cuts at: 4783 5161 4783
Size: 378 7096
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3276 3276
Size: 7474
FspI TGC'GCA
Cuts at: 5980 5980
Size: 7474
HaeII r_GCGC'y
Cuts at: 1678 4464 6621 1678
Size: 2786 2157 2531
Fragments arranged by size:
2786 2531 2157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 2516 2548 2561
Size: 32 135 32 781 32 131 32 13
Cuts at: 2561 2593 2615 2647 3097 3129 3450 3482 3787
Size: 32 22 32 450 32 321 32 305
Cuts at: 3787 3819 3931 3963 4526 4551 4558 4583 6115
Size: 32 112 32 563 25 7 25 1532
Cuts at: 6115 6147 6189 6221 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1532 781 563 450 321 305 135
131 112 42 32 32 32 32 32
32 32 32 32 32 32 32 25
25 22 13 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4342 4818 5064 669
Size: 988 281 2404 476 246 3079
Fragments arranged by size:
3079 2404 988 476 281 246
HindIII A'AGCT_T
Cuts at: 748 2789 3893 748
Size: 2041 1104 4329
Fragments arranged by size:
4329 2041 1104
HpaI GTT'AAC
Cuts at: 5064 5064
Size: 7474
KasI G'GCGC_C
Cuts at: 4460 4460
Size: 7474
KpnI G_GTAC'C
Cuts at: 3957 4799 3957
Size: 842 6632
MfeI C'AATT_G
Cuts at: 5073 5073
Size: 7474
MluI A'CGCG_T
Cuts at: 7350 7350
Size: 7474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2048 2328 2514 3627 4193 6588 6772 1305
Size: 743 280 186 1113 566 2395 184 2007
Fragments arranged by size:
2395 2007 1113 743 566 280 186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3454 10
Size: 54 1425 343 1622 4030
Fragments arranged by size:
4030 1622 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5432 5791 5950 518
Size: 648 4266 359 159 2042
Fragments arranged by size:
4266 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2147 2222 3557 3713 3918 4000 4710
Size: 425 116 75 1335 156 205 82 710
Cuts at: 4710 4890 5567 6715 6960 1606
Size: 180 677 1148 245 2120
Fragments arranged by size:
2120 1335 1148 710 677 425 245 205
180 156 116 82 75
NarI GG'CG_CC
Cuts at: 4461 4461
Size: 7474
NcoI C'CATG_G
Cuts at: 513 1065 3358 513
Size: 552 2293 4629
Fragments arranged by size:
4629 2293 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7474
NotI GC'GGCC_GC
Cuts at: 4921 4921
Size: 7474
NspI r_CATG'y
Cuts at: 1132 1229 4420 4832 6377 1132
Size: 97 3191 412 1545 2229
Fragments arranged by size:
3191 2229 1545 412 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7474
PciI A'CATG_T
Cuts at: 4416 6373 4416
Size: 1957 5517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3016 3461 1108
Size: 1908 445 5121
Fragments arranged by size:
5121 1908 445
PfoI T'CCnGG_A
Cuts at: 2092 3417 4146 4530 2092
Size: 1325 729 384 5036
Fragments arranged by size:
5036 1325 729 384
PmeI GTTT'AAAC
Cuts at: 4778 4778
Size: 7474
PmlI CAC'GTG
Cuts at: 2650 2650
Size: 7474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5543 5575 7056 7088 5543
Size: 32 1481 32 5929
Fragments arranged by size:
5929 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4052 4209 1613
Size: 2439 157 4878
Fragments arranged by size:
4878 2439 157
PsiI TTA'TAA
Cuts at: 3891 5044 3891
Size: 1153 6321
PspXI vC'TCGA_Gb
Cuts at: 3564 3564
Size: 7474
PstI C_TGCA'G
Cuts at: 830 2112 2832 3070 4355 4524 4762 4826 830
Size: 1282 720 238 1285 169 238 64 3478
Fragments arranged by size:
3478 1285 1282 720 238 238 169 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7474
PvuII CAG'CTG
Cuts at: 2147 2222 3557 4000 4710 2147
Size: 75 1335 443 710 4911
Fragments arranged by size:
4911 1335 710 443 75
SacI G_AGCT'C
Cuts at: 2330 2330
Size: 7474
SacII CC_GC'GG
Cuts at: 2032 2032
Size: 7474
SalI G'TCGA_C
Cuts at: 1936 4816 1936
Size: 2880 4594
SapI GCTCTTCn'nnn_
Cuts at: 2113 2113
Size: 7474
SbfI CC_TGCA'GG
Cuts at: 4524 4826 4524
Size: 302 7172
ScaI AGT'ACT
Cuts at: 5722 7434 5722
Size: 1712 5762
SfcI C'TryA_G
Cuts at: 826 1045 2108 2556 2828 3066 3339 3686 4351
Size: 219 1063 448 272 238 273 347 665
Cuts at: 4351 4520 4758 4822 5957 6638 6829 826
Size: 169 238 64 1135 681 191 1471
Fragments arranged by size:
1471 1135 1063 681 665 448 347 273
272 238 238 219 191 169 64
SfoI GGC'GCC
Cuts at: 4462 4462
Size: 7474
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
SgrAI Cr'CCGG_yG
Cuts at: 1700 4613 1700
Size: 2913 4561
SmaI CCC'GGG
Cuts at: 1872 4487 4801 1872
Size: 2615 314 4545
Fragments arranged by size:
4545 2615 314
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3564 3922 4508 4543 5578 6479
Size: 197 926 1621 358 586 35 1035 901
Cuts at: 6479 6741 820
Size: 262 1553
Fragments arranged by size:
1621 1553 1035 926 901 586 358 262
197 35
SnaBI TAC'GTA
Cuts at: 493 4771 5265 493
Size: 4278 494 2702
Fragments arranged by size:
4278 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7474
SphI G_CATG'C
Cuts at: 1132 4832 1132
Size: 3700 3774
SspI AAT'ATT
Cuts at: 5 52 5398 5
Size: 47 5346 2081
Fragments arranged by size:
5346 2081 47
StuI AGG'CCT
Cuts at: 1517 3028 1517
Size: 1511 5963
StyI C'CwwG_G
Cuts at: 513 1065 2699 3057 3358 4913 513
Size: 552 1634 358 301 1555 3074
Fragments arranged by size:
3074 1634 1555 552 358 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4064 5501 5518 5671 5856 6301 325
Size: 1255 2484 1437 17 153 185 445 1498
Fragments arranged by size:
2484 1498 1437 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 5720 7432
Size: 274 80 33 51 1443 1245 2498 1712
Cuts at: 7432 96
Size: 138
Fragments arranged by size:
2498 1712 1443 1245 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2317 2349 3294 3326 3456 3488 3829 3861 4207
Size: 32 945 32 130 32 341 32 346
Cuts at: 4207 4239 4644 4676 7434 7466 2317
Size: 32 405 32 2758 32 2325
Fragments arranged by size:
2758 2325 945 405 346 341 130 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3098 1316
Size: 1782 5692
XbaI T'CTAG_A
Cuts at: 4810 4810
Size: 7474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4102 4102
Size: 7474
XhoI C'TCGA_G
Cuts at: 1943 3564 1943
Size: 1621 5853
XmaI C'CCGG_G
Cuts at: 1870 4485 4799 1870
Size: 2615 314 4545
Fragments arranged by size:
4545 2615 314
XmnI GAAnn'nnTTC
Cuts at: 2187 5603 2187
Size: 3416 4058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5282 276
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PpuMI PspOMI PsrI RsrII SanDI SexAI
SfiI SrfI SwaI