Restriction map for
pFN21ASDA0142
(Circular) (Six-base) MAPSORT of: pFN21ASDA0142.seq Check: 6153 from: 1 to: 6667
pFN21ASDA0142 6667 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 4477 278
Size: 53 83 186 936 582 2359 2468
Fragments arranged by size:
2468 2359 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4010 1937
Size: 2073 4594
Acc65I G'GTAC_C
Cuts at: 3988 3988
Size: 6667
AclI AA'CG_TT
Cuts at: 1563 4794 5167 1563
Size: 3231 373 3063
Fragments arranged by size:
3231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2342 3552 3941 4730 6114 6382 1632
Size: 641 69 1210 389 789 1384 268 1917
Fragments arranged by size:
1917 1384 1210 789 641 389 268 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6470
AflIII A'CryG_T
Cuts at: 1203 2934 5566 6543 1203
Size: 1731 2632 977 1327
Fragments arranged by size:
2632 1731 1327 977
AgeI A'CCGG_T
Cuts at: 5498 5498
Size: 6667
AhdI GACnn_n'nnGTC
Cuts at: 5396 5396
Size: 6667
AleI CACnn'nnGTG
Cuts at: 1166 2132 3230 1166
Size: 966 1098 4603
Fragments arranged by size:
4603 1098 966
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 2519 1184
Size: 1032 303 5332
Fragments arranged by size:
5332 1032 303
AlwNI CAG_nnn'CTG
Cuts at: 2872 2947 3184 3628 4410 5982 2872
Size: 75 237 444 782 1572 3557
Fragments arranged by size:
3557 1572 782 444 237 75
ApaLI G'TGCA_C
Cuts at: 4724 5880 4724
Size: 1156 5511
ApoI r'AATT_y
Cuts at: 1493 3976 4202 4354 6463 1493
Size: 2483 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5221 160
Size: 872 4189 1606
Fragments arranged by size:
4189 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 3992 1870
Size: 73 804 1245 4545
Fragments arranged by size:
4545 1245 804 73
AvrII C'CTAG_G
Cuts at: 3032 3032
Size: 6667
BamHI G'GATC_C
Cuts at: 3997 4364 3997
Size: 367 6300
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2657 3377 3988 5443 618
Size: 325 217 627 870 720 611 1455 1842
Fragments arranged by size:
1842 1455 870 720 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2261 2748 3518 3886 3986 1391
Size: 870 487 770 368 100 4072
Fragments arranged by size:
4072 870 770 487 368 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5696
BbvCI CC'TCA_GC
Cuts at: 3478 3478
Size: 6667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4858 4892 1903
Size: 34 2921 34 3678
Fragments arranged by size:
3678 2921 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3790 4559 5769 3790
Size: 769 1210 4688
Fragments arranged by size:
4688 1210 769
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6667
BfrBI ATG'CAT
Cuts at: 3122 3809 3122
Size: 687 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 5278 136
Size: 107 122 71 631 605 506 3100 1525
Fragments arranged by size:
3100 1525 631 605 506 122 107 71
BglII A'GATC_T
Cuts at: 6662 6662
Size: 6667
BlpI GC'TnA_GC
Cuts at: 2514 4084 2514
Size: 1570 5097
Bme1580I G_kGCm'C
Cuts at: 1790 2162 4728 5884 1790
Size: 372 2566 1156 2573
Fragments arranged by size:
2573 2566 1156 372
BmgBI CAC'GTC
Cuts at: 1318 3228 1318
Size: 1910 4757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2902 3409 4398
Size: 416 53 83 399 24 1485 507 989
Cuts at: 4398 5356 442
Size: 958 1753
Fragments arranged by size:
1753 1485 989 958 507 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3431 3457 3917 5327 1239
Size: 2192 26 460 1410 2579
Fragments arranged by size:
2579 2192 1410 460 26
Bpu10I CC'TnA_GC
Cuts at: 2696 3443 3478 2696
Size: 747 35 5885
Fragments arranged by size:
5885 747 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3116 4792 5657 5955 2879
Size: 237 1676 865 298 3591
Fragments arranged by size:
3591 1676 865 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2642 3944 5330 882
Size: 617 1143 1302 1386 2219
Fragments arranged by size:
2219 1386 1302 1143 617
BsaAI yAC'GTr
Cuts at: 493 1204 2200 2577 3964 4458 493
Size: 711 996 377 1387 494 2702
Fragments arranged by size:
2702 1387 996 711 494 377
BsaBI GATnn'nnATC
Cuts at: 6661 6661
Size: 6667
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3338 4474 4856
Size: 53 83 186 936 582 1223 1136 382
Cuts at: 4856 5491 275
Size: 635 1451
Fragments arranged by size:
1451 1223 1136 936 635 582 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2841 2886 5100 5498 5772 5919 6330 2841
Size: 45 2214 398 274 147 411 3178
Fragments arranged by size:
3178 2214 411 398 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3569 3599 1178
Size: 30 165 30 2166 30 4246
Fragments arranged by size:
4246 2166 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2642 3590 1186
Size: 1456 948 4263
Fragments arranged by size:
4263 1456 948
BseYI C'CCAG_C
Cuts at: 2189 5870 6366 2189
Size: 3681 496 2490
Fragments arranged by size:
3681 2490 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3634 1163
Size: 573 1898 4196
Fragments arranged by size:
4196 1898 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2173 4117 4878 5906 1540
Size: 275 186 172 1944 761 1028 2301
Fragments arranged by size:
2301 1944 1028 761 275 186 172
BsiHKAI G_wGCw'C
Cuts at: 2748 3204 4728 4813 5884 2748
Size: 456 1524 85 1071 3531
Fragments arranged by size:
3531 1524 1071 456 85
BsmI GAATG_Cn'
Cuts at: 2498 4178 4271 2498
Size: 1680 93 4894
Fragments arranged by size:
4894 1680 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2106 2106
Size: 6667
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2261 2748 3204 3518 3886 3986
Size: 399 372 99 487 456 314 368 100
Cuts at: 3986 4728 4813 5884 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 456 399 372 368
314 100 99 85
BspEI T'CCGG_A
Cuts at: 2841 2886 6330 2841
Size: 45 3444 3178
Fragments arranged by size:
3444 3178 45
BspHI T'CATG_A
Cuts at: 4556 6286 4556
Size: 1730 4937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4022 844
Size: 3178 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3151 4554 1394
Size: 429 1328 1403 3507
Fragments arranged by size:
3507 1403 1328 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2387 5162 5336 65
Size: 1183 1139 2775 174 1396
Fragments arranged by size:
2775 1396 1183 1139 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2177 5311 5498 1700
Size: 110 367 3134 187 2869
Fragments arranged by size:
3134 2869 367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6667
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6667
BssSI C'ACGA_G
Cuts at: 2678 4727 5739 2678
Size: 2049 1012 3606
Fragments arranged by size:
3606 2049 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3184 794
Size: 2390 4277
BstBI TT'CG_AA
Cuts at: 2007 5471 2007
Size: 3464 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6667
BstYI r'GATC_y
Cuts at: 1972 2868 3181 3502 3997 4364 4749 4766 6218
Size: 896 313 321 495 367 385 17 1452
Cuts at: 6218 6304 6316 6662 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 896 495 385 367 346 321
313 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2688 2799 3373 513
Size: 552 1623 111 574 3807
Fragments arranged by size:
3807 1623 574 552 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2916 487
Size: 683 56 1690 4238
Fragments arranged by size:
4238 1690 683 56
BtsI GCAGTG_nn'
Cuts at: 2607 4183 4977 4997 2607
Size: 1576 794 20 4277
Fragments arranged by size:
4277 1576 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3696 3731 529
Size: 35 3132 35 3465
Fragments arranged by size:
3465 3132 35 35
DraI TTT'AAA
Cuts at: 2541 3971 2541
Size: 1430 5237
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5674 809
Size: 507 4358 1802
Fragments arranged by size:
4358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2170 2384 4114
Size: 54 1425 50 275 18 340 214 1730
Cuts at: 4114 5003 6632 8
Size: 889 1629 43
Fragments arranged by size:
1730 1629 1425 889 340 275 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2170 4114 1537
Size: 275 358 1944 4090
Fragments arranged by size:
4090 1944 358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2249 2840 3002 3723 4597 1353
Size: 780 116 591 162 721 874 3423
Fragments arranged by size:
3423 874 780 721 591 162 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5254 5628 5774 387
Size: 1064 138 3665 374 146 1280
Fragments arranged by size:
3665 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2746 2746
Size: 6667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2342 3431 3457 3552 3917 3941
Size: 393 641 69 1089 26 95 365 24
Cuts at: 3941 4730 5327 6114 6382 1239
Size: 789 597 787 268 1524
Fragments arranged by size:
1524 1089 789 787 641 597 393 365
268 95 69 26 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2265 2265
Size: 6667
EcoO109I rG'GnC_Cy
Cuts at: 2944 3404 2944
Size: 460 6207
EcoRI G'AATT_C
Cuts at: 3976 4354 3976
Size: 378 6289
EcoRV GAT'ATC
Cuts at: 2987 2987
Size: 6667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 3734 2086
Size: 1648 5019
FspI TGC'GCA
Cuts at: 5173 5173
Size: 6667
HaeII r_GCGC'y
Cuts at: 1678 3660 5814 1678
Size: 1982 2154 2531
Fragments arranged by size:
2531 2154 1982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 3569 3601 5308
Size: 32 135 32 531 32 1434 32 1707
Cuts at: 5308 5340 5382 5414 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1707 1434 531 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4011 4257 669
Size: 988 281 2073 246 3079
Fragments arranged by size:
3079 2073 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3062 3092 3642 748
Size: 2314 30 550 3773
Fragments arranged by size:
3773 2314 550 30
HpaI GTT'AAC
Cuts at: 4257 4257
Size: 6667
KpnI G_GTAC'C
Cuts at: 3992 3992
Size: 6667
MfeI C'AATT_G
Cuts at: 4266 4266
Size: 6667
MluI A'CGCG_T
Cuts at: 6543 6543
Size: 6667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2506 3092 5781 5965 1305
Size: 1201 586 2689 184 2007
Fragments arranged by size:
2689 2007 1201 586 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4845
Fragments arranged by size:
4845 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2933 3117 3230 4625 4984 5143
Size: 648 966 801 184 113 1395 359 159
Cuts at: 5143 518
Size: 2042
Fragments arranged by size:
2042 1395 966 801 648 359 184 159
113
MspA1I CmG'CkG
Cuts at: 1606 3334 3482 3602 4083 4760 5908 6153 1606
Size: 1728 148 120 481 677 1148 245 2120
Fragments arranged by size:
2120 1728 1148 677 481 245 148 120
NcoI C'CATG_G
Cuts at: 513 1065 2799 513
Size: 552 1734 4381
Fragments arranged by size:
4381 1734 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6667
NotI GC'GGCC_GC
Cuts at: 4114 4114
Size: 6667
NsiI A_TGCA'T
Cuts at: 3124 3811 3124
Size: 687 5980
NspI r_CATG'y
Cuts at: 1132 1229 2938 4025 5570 1132
Size: 97 1709 1087 1545 2229
Fragments arranged by size:
2229 1709 1545 1087 97
PasI CC'CwG_GG
Cuts at: 1348 2669 1348
Size: 1321 5346
PciI A'CATG_T
Cuts at: 2934 5566 2934
Size: 2632 4035
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3399 3435 1108
Size: 2291 36 4340
Fragments arranged by size:
4340 2291 36
PfoI T'CCnGG_A
Cuts at: 3587 3587
Size: 6667
PmeI GTTT'AAAC
Cuts at: 3971 3971
Size: 6667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 6249 6281 4736
Size: 32 1481 32 5122
Fragments arranged by size:
5122 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2944 3404 2944
Size: 460 6207
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6667
PsiI TTA'TAA
Cuts at: 4237 4237
Size: 6667
PstI C_TGCA'G
Cuts at: 830 2602 2868 3189 4019 830
Size: 1772 266 321 830 3478
Fragments arranged by size:
3478 1772 830 321 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6667
PvuII CAG'CTG
Cuts at: 3482 3602 3482
Size: 120 6547
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 6667
SacI G_AGCT'C
Cuts at: 2748 2748
Size: 6667
SalI G'TCGA_C
Cuts at: 1936 4009 1936
Size: 2073 4594
SanDI GG'GwC_CC
Cuts at: 3404 3404
Size: 6667
SapI GCTCTTCn'nnn_
Cuts at: 2249 2249
Size: 6667
SbfI CC_TGCA'GG
Cuts at: 4019 4019
Size: 6667
ScaI AGT'ACT
Cuts at: 4915 6627 4915
Size: 1712 4955
SfcI C'TryA_G
Cuts at: 826 1045 2315 2598 2864 3185 3206 4015 5150
Size: 219 1270 283 266 321 21 809 1135
Cuts at: 5150 5831 6022 826
Size: 681 191 1471
Fragments arranged by size:
1471 1270 1135 809 681 321 283 266
219 191 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6667
SmaI CCC'GGG
Cuts at: 1872 3994 1872
Size: 2122 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 2858 3095 4771 5672 5934
Size: 197 926 804 111 237 1676 901 262
Cuts at: 5934 820
Size: 1553
Fragments arranged by size:
1676 1553 926 901 804 262 237 197
111
SnaBI TAC'GTA
Cuts at: 493 3964 4458 493
Size: 3471 494 2702
Fragments arranged by size:
3471 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6667
SphI G_CATG'C
Cuts at: 1132 4025 1132
Size: 2893 3774
SspI AAT'ATT
Cuts at: 5 52 2323 2345 4591 5
Size: 47 2271 22 2246 2081
Fragments arranged by size:
2271 2246 2081 47 22
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6667
StyI C'CwwG_G
Cuts at: 513 1065 2799 3032 4106 513
Size: 552 1734 233 1074 3074
Fragments arranged by size:
3074 1734 1074 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 3184 3442 4694 4711 4864 5049
Size: 1255 462 1142 258 1252 17 153 185
Cuts at: 5049 5494 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1252 1142 462 445 258 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4913 6625 96
Size: 274 80 33 51 1443 2936 1712 138
Fragments arranged by size:
2936 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6627 6659 6627
Size: 32 6635
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6667
XbaI T'CTAG_A
Cuts at: 2443 3865 4003 2443
Size: 1422 138 5107
Fragments arranged by size:
5107 1422 138
XhoI C'TCGA_G
Cuts at: 1943 2747 1943
Size: 804 5863
XmaI C'CCGG_G
Cuts at: 1870 3992 1870
Size: 2122 4545
XmnI GAAnn'nnTTC
Cuts at: 4796 4796
Size: 6667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 4475 276
Size: 53 83 186 936 582 2359 2468
Fragments arranged by size:
2468 2359 936 582 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI SacII SexAI SfiI SfoI SrfI SwaI XcmI