Restriction map for
pFN21ASDA0140
(Circular) (Six-base) MAPSORT of: pFN21ASDA0140.seq Check: 9437 from: 1 to: 5995
pFN21ASDA0140 5995 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2633 2779 2633
Size: 146 5849
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2071 2180 2482 2509 2898 2926 4058 5442
Size: 439 109 302 27 389 28 1132 1384
Cuts at: 5442 5710 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1132 439 389 302 268 109
28 27
AfeI AGC'GCT
Cuts at: 2483 2483
Size: 5995
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 2223 3027 3243 3738 5310 2223
Size: 804 216 495 1572 2908
Fragments arranged by size:
2908 1572 804 495 216
ApaI G_GGCC'C
Cuts at: 3229 3229
Size: 5995
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 3304 3530 3682 5791 1493
Size: 1811 226 152 2109 1697
Fragments arranged by size:
2109 1811 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2538 3320 1870
Size: 73 112 483 782 4545
Fragments arranged by size:
4545 782 483 112 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 2614 2871 3316 4771
Size: 325 217 627 472 355 257 445 1455
Cuts at: 4771 618
Size: 1842
Fragments arranged by size:
1842 1455 627 472 445 355 325 257
217
BanII G_rGCy'C
Cuts at: 1391 2426 2660 2996 3229 3314 1391
Size: 1035 234 336 233 85 4072
Fragments arranged by size:
4072 1035 336 234 233 85
BbeI G_GCGC'C
Cuts at: 2875 2875
Size: 5995
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BbvCI CC'TCA_GC
Cuts at: 2048 2309 2338 2755 2951 2048
Size: 261 29 417 196 5092
Fragments arranged by size:
5092 417 261 196 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 2298 1548
Size: 750 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2921 3060 4606
Size: 107 122 71 631 605 1249 139 1546
Cuts at: 4606 136
Size: 1525
Fragments arranged by size:
1546 1525 1249 631 605 139 122 107
71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 2623 3412 2623
Size: 789 5206
Bme1580I G_kGCm'C
Cuts at: 1790 3229 4056 5212 1790
Size: 1439 827 1156 2573
Fragments arranged by size:
2573 1439 1156 827
BmgBI CAC'GTC
Cuts at: 1318 3010 1318
Size: 1692 4303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2919 2919
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2674 2785 4655 1239
Size: 1435 111 1870 2579
Fragments arranged by size:
2579 1870 1435 111
Bpu10I CC'TnA_GC
Cuts at: 2048 2309 2338 2755 2951 2960 3090 2048
Size: 261 29 417 196 9 130 4953
Fragments arranged by size:
4953 417 261 196 130 29 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4120 4985 5283 4120
Size: 865 298 4832
Fragments arranged by size:
4832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2166 4658 882
Size: 617 667 2492 2219
Fragments arranged by size:
2492 2219 667 617
BsaAI yAC'GTr
Cuts at: 493 1204 3292 3786 493
Size: 711 2088 494 2702
Fragments arranged by size:
2702 2088 711 494
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2872 3802 4184 4819
Size: 53 83 186 936 1339 930 382 635
Cuts at: 4819 275
Size: 1451
Fragments arranged by size:
1451 1339 936 930 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4428 4826 5100 5247 5658 4428
Size: 398 274 147 411 4765
Fragments arranged by size:
4765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 3125 3155 1178
Size: 30 165 30 1134 30 558 30 4018
Fragments arranged by size:
4018 1134 558 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2527 2545 2694 3164 1186
Size: 1341 18 149 470 4017
Fragments arranged by size:
4017 1341 470 149 18
BseYI C'CCAG_C
Cuts at: 2826 3074 5198 5694 2826
Size: 248 2124 496 3127
Fragments arranged by size:
3127 2124 496 248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2651 1163
Size: 573 348 567 4507
Fragments arranged by size:
4507 573 567 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 2553 4056 4141 5212 2216
Size: 337 1503 85 1071 2999
Fragments arranged by size:
2999 1503 1071 337 85
BsmI GAATG_Cn'
Cuts at: 2234 3506 3599 2234
Size: 1272 93 4630
Fragments arranged by size:
4630 1272 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2446 2788 2446
Size: 342 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2426 2553 2660 2996 3229 3314
Size: 399 426 210 127 107 336 233 85
Cuts at: 3314 4056 4141 5212 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 336 233 210
127 107 85 85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2633 2673 2703 2779 3350 844
Size: 1789 40 30 76 571 3489
Fragments arranged by size:
3489 1789 571 76 40 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2367 2823 3194 3882 1394
Size: 429 544 456 371 688 3507
Fragments arranged by size:
3507 688 544 456 429 371
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 4490 4664 65
Size: 1183 822 2420 174 1396
Fragments arranged by size:
2420 1396 1183 822 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2685 3199 4639 4826 1700
Size: 110 875 514 1440 187 2869
Fragments arranged by size:
2869 1440 875 514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 2007 4799 2007
Size: 2792 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2591 2029
Size: 562 5433
BstYI r'GATC_y
Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990
Size: 1353 367 385 17 1452 86 12 346
Cuts at: 5990 1972
Size: 1977
Fragments arranged by size:
1977 1452 1353 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2442 2673 3220 513
Size: 552 957 420 231 547 3288
Fragments arranged by size:
3288 957 552 547 420 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 3511 4305 4325 2277
Size: 1234 794 20 3947
Fragments arranged by size:
3947 1234 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DraIII CAC_nnn'GTG
Cuts at: 2674 2674
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2852 3122 5002 809
Size: 507 1536 270 1880 1802
Fragments arranged by size:
1880 1802 1536 507 270
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3442 4331 5960
Size: 54 1425 50 275 18 1612 889 1629
Cuts at: 5960 8
Size: 43
Fragments arranged by size:
1629 1612 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 3102 3925 1353
Size: 846 903 823 3423
Fragments arranged by size:
3423 903 846 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2180 2482 2509 2674 2785 2898
Size: 393 439 109 302 27 165 111 113
Cuts at: 2898 2926 4058 4655 5442 5710 1239
Size: 28 1132 597 787 268 1524
Fragments arranged by size:
1524 1132 787 597 439 393 302 268
165 113 111 109 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3173 3173
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 2082 2126 2415 3024 3066 3078 3136 3225 3226
Size: 44 289 609 42 12 58 89 1
Cuts at: 3226 2082
Size: 4851
Fragments arranged by size:
4851 609 289 89 58 44 42 12
1
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2359 2485 2875 5142 1678
Size: 681 126 390 2267 2531
Fragments arranged by size:
2531 2267 681 390 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3125 3157 4636
Size: 32 135 32 803 32 718 32 1479
Cuts at: 4636 4668 4710 4742 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1479 803 718 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KasI G'GCGC_C
Cuts at: 2871 2871
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 3243 5109 5293 1305
Size: 1209 729 1866 184 2007
Fragments arranged by size:
2007 1866 1209 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 3953 4312 4471 518
Size: 648 861 1926 359 159 2042
Fragments arranged by size:
2042 1926 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2466 2701 2805 2955 3074 3162 3222 3411
Size: 860 235 104 150 119 88 60 189
Cuts at: 3411 4088 5236 5481 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 860 677 245 235 189 150
119 104 88 60
NaeI GCC'GGC
Cuts at: 2687 3201 2687
Size: 514 5481
NarI GG'CG_CC
Cuts at: 2872 2872
Size: 5995
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4486
Fragments arranged by size:
4486 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NgoMIV G'CCGG_C
Cuts at: 2685 3199 2685
Size: 514 5481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2079 3353 4898 1132
Size: 97 850 1274 1545 2229
Fragments arranged by size:
2229 1545 1274 850 97
PasI CC'CwG_GG
Cuts at: 1348 3056 3230 1348
Size: 1708 174 4113
Fragments arranged by size:
4113 1708 174
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 2433 3027 1108
Size: 1115 210 594 4076
Fragments arranged by size:
4076 1115 594 210
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2126 3066 3078 3136 2082
Size: 44 940 12 58 4941
Fragments arranged by size:
4941 940 58 44 12
PshAI GACnn'nnGTC
Cuts at: 1613 2982 3083 1613
Size: 1369 101 4525
Fragments arranged by size:
4525 1369 101
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 3225 3225
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2700 3208 3347 830
Size: 1870 508 139 3478
Fragments arranged by size:
3478 1870 508 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2955 3162 2955
Size: 207 5788
SacII CC_GC'GG
Cuts at: 3223 3223
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SanDI GG'GwC_CC
Cuts at: 3078 3078
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2461 2696 3204 3343 4478 5159 5350
Size: 219 1416 235 508 139 1135 681 191
Cuts at: 5350 826
Size: 1471
Fragments arranged by size:
1471 1416 1135 681 508 235 219 191
139
SfoI GGC'GCC
Cuts at: 2873 2873
Size: 5995
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 2057 2540 3322 1872
Size: 185 483 782 4545
Fragments arranged by size:
4545 782 483 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4099 5000 5262 820
Size: 197 926 2156 901 262 1553
Fragments arranged by size:
2156 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3292 3786 493
Size: 2799 494 2702
Fragments arranged by size:
2799 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 2899 1517
Size: 1382 4613
StyI C'CwwG_G
Cuts at: 513 1065 2022 2418 2608 3434 513
Size: 552 957 396 190 826 3074
Fragments arranged by size:
3074 957 826 552 396 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4022 4039 4192 4377 4822 325
Size: 1255 2442 17 153 185 445 1498
Fragments arranged by size:
2442 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4241 5953 96
Size: 274 80 33 51 1443 2264 1712 138
Fragments arranged by size:
2264 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 2905 3331 2905
Size: 426 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2588 2588
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 2055 2538 3320 1870
Size: 185 483 782 4545
Fragments arranged by size:
4545 782 483 185
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI
SfiI SrfI SwaI