Restriction map for
pFN21ASDA0139
(Circular) (Six-base) MAPSORT of: pFN21ASDA0139.seq Check: 651 from: 1 to: 8875
pFN21ASDA0139 8875 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3337 3337
Size: 8875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6685 278
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 6218 1937
Size: 1041 3240 4594
Fragments arranged by size:
4594 3240 1041
Acc65I G'GTAC_C
Cuts at: 6196 6196
Size: 8875
AclI AA'CG_TT
Cuts at: 1563 4129 7002 7375 1563
Size: 2566 2873 373 3063
Fragments arranged by size:
3063 2873 2566 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 3552 4752 5722 6938 8322 8590 1632
Size: 714 1206 1200 970 1216 1384 268 1917
Fragments arranged by size:
1917 1384 1216 1206 1200 970 714 268
AfeI AGC'GCT
Cuts at: 4159 4159
Size: 8875
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8678
AflIII A'CryG_T
Cuts at: 1203 2588 7774 8751 1203
Size: 1385 5186 977 1327
Fragments arranged by size:
5186 1385 1327 977
AgeI A'CCGG_T
Cuts at: 7706 7706
Size: 8875
AhdI GACnn_n'nnGTC
Cuts at: 5537 7604 5537
Size: 2067 6808
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8875
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4934 4966 4934
Size: 32 8843
AlwNI CAG_nnn'CTG
Cuts at: 4742 6618 8190 4742
Size: 1876 1572 5427
Fragments arranged by size:
5427 1876 1572
ApaI G_GGCC'C
Cuts at: 5438 5438
Size: 8875
ApaLI G'TGCA_C
Cuts at: 6932 8088 6932
Size: 1156 7719
ApoI r'AATT_y
Cuts at: 1493 2072 2474 2570 2600 3212 3700 4140 5403
Size: 579 402 96 30 612 488 440 1263
Cuts at: 5403 6184 6410 6562 8671 1493
Size: 781 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 781 612 579 488 440
402 226 152 96 30
AseI AT'TA_AT
Cuts at: 160 1032 3135 7429 160
Size: 872 2103 4294 1606
Fragments arranged by size:
4294 2103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
AvaI C'yCGr_G
Cuts at: 1870 1943 3525 5598 6200 1870
Size: 73 1582 2073 602 4545
Fragments arranged by size:
4545 2073 1582 602 73
AvrII C'CTAG_G
Cuts at: 5747 5747
Size: 8875
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4535 4568 4535
Size: 33 8842
BamHI G'GATC_C
Cuts at: 3723 4015 6205 6572 3723
Size: 292 2190 367 6026
Fragments arranged by size:
6026 2190 367 292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3968 4712 6196 7651 618
Size: 325 217 627 2181 744 1484 1455 1842
Fragments arranged by size:
2181 1842 1484 1455 744 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4866 5438 6194 1391
Size: 3475 572 756 4072
Fragments arranged by size:
4072 3475 756 572
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2433 3095 4005 4293 4657 5040 5749
Size: 971 534 662 910 288 364 383 709
Cuts at: 5749 928
Size: 4054
Fragments arranged by size:
4054 971 910 709 662 534 383 364
288
BbvCI CC'TCA_GC
Cuts at: 3843 5781 3843
Size: 1938 6937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7066 7100 1903
Size: 34 5129 34 3678
Fragments arranged by size:
5129 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6767 7977 6767
Size: 1210 7665
BclI T'GATC_A
Cuts at: 1548 3475 1548
Size: 1927 6948
BfrBI ATG'CAT
Cuts at: 2707 2900 2707
Size: 193 8682
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 7486 136
Size: 107 122 71 631 605 3315 2499 1525
Fragments arranged by size:
3315 2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5884 8870 5884
Size: 2986 5889
BlpI GC'TnA_GC
Cuts at: 6292 6292
Size: 8875
Bme1580I G_kGCm'C
Cuts at: 1790 5438 6936 8092 1790
Size: 3648 1498 1156 2573
Fragments arranged by size:
3648 2573 1498 1156
BmgBI CAC'GTC
Cuts at: 1318 4781 4931 5141 5474 5957 1318
Size: 3463 150 210 333 483 4236
Fragments arranged by size:
4236 3463 483 333 210 150
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3974 6606 7564
Size: 416 53 83 399 24 2557 2632 958
Cuts at: 7564 442
Size: 1753
Fragments arranged by size:
2632 2557 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6100 6100
Size: 8875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 3809 4073 4738 5785 5818 7535 1239
Size: 1061 1509 264 665 1047 33 1717 2579
Fragments arranged by size:
2579 1717 1509 1061 1047 665 264 33
Bpu10I CC'TnA_GC
Cuts at: 3021 3843 5781 3021
Size: 822 1938 6115
Fragments arranged by size:
6115 1938 822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 3788 4025 5497 5791 7000 7865 8163 2099
Size: 1689 237 1472 294 1209 865 298 2811
Fragments arranged by size:
2811 1689 1472 1209 865 298 294 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4812 5524 6027 6039 6045 7538 882
Size: 617 3313 712 503 12 6 1493 2219
Fragments arranged by size:
3313 2219 1493 712 617 503 12 6
BsaAI yAC'GTr
Cuts at: 493 1204 6172 6666 493
Size: 711 4968 494 2702
Fragments arranged by size:
4968 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 5247 5367 5457 5484 5721 8869 5247
Size: 120 90 27 237 3148 5253
Fragments arranged by size:
5253 3148 237 120 90 27
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4837 5050 6682 7064
Size: 53 83 186 936 3304 213 1632 382
Cuts at: 7064 7699 275
Size: 635 1451
Fragments arranged by size:
3304 1632 1451 936 635 382 213 186
83 53
BsaWI w'CCGG_w
Cuts at: 3286 7308 7706 7980 8127 8538 3286
Size: 4022 398 274 147 411 3623
Fragments arranged by size:
4022 3623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8650
Fragments arranged by size:
8650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3764 4472 4475 5187 5594 5687 5991 1186
Size: 2578 708 3 712 407 93 304 4070
Fragments arranged by size:
4070 2578 712 708 407 304 93 3
BseYI C'CCAG_C
Cuts at: 2556 5586 6019 8078 8574 2556
Size: 3030 433 2059 496 2857
Fragments arranged by size:
3030 2857 2059 496 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4305 5439 1163
Size: 573 2569 1134 4599
Fragments arranged by size:
4599 2569 1134 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6325 7086 8114 1540
Size: 275 186 4324 761 1028 2301
Fragments arranged by size:
4324 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 6936 7021 8092 3717
Size: 3219 85 1071 4500
Fragments arranged by size:
4500 3219 1071 85
BsiWI C'GTAC_G
Cuts at: 3026 3026
Size: 8875
BsmI GAATG_Cn'
Cuts at: 2634 2768 3977 4284 6386 6479 2634
Size: 134 1209 307 2102 93 5030
Fragments arranged by size:
5030 2102 1209 307 134 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4771 5886 5947 4771
Size: 1115 61 7699
Fragments arranged by size:
7699 1115 61
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3717 4866 5438 6194 6936 7021 8092
Size: 399 1927 1149 572 756 742 85 1071
Cuts at: 8092 1391
Size: 2174
Fragments arranged by size:
2174 1927 1149 1071 756 742 572 399
85
BspEI T'CCGG_A
Cuts at: 8538 8538
Size: 8875
BspHI T'CATG_A
Cuts at: 6764 8494 6764
Size: 1730 7145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3337 3454 6230 844
Size: 2493 117 2776 3489
Fragments arranged by size:
3489 2776 2493 117
BsrBI CCG'CTC
Cuts at: 1394 1823 4498 4561 4828 5992 6762 1394
Size: 429 2675 63 267 1164 770 3507
Fragments arranged by size:
3507 2675 1164 770 429 267 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 3451 7370 7544 65
Size: 1183 2203 3919 174 1396
Fragments arranged by size:
3919 2203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 4977 5859 7519 7706 1700
Size: 110 216 2951 882 1660 187 2869
Fragments arranged by size:
2951 2869 1660 882 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8875
BssHII G'CGCG_C
Cuts at: 1925 4513 1925
Size: 2588 6287
BssSI C'ACGA_G
Cuts at: 6935 7947 6935
Size: 1012 7863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3304 3562 794
Size: 2510 258 6107
Fragments arranged by size:
6107 2510 258
BstBI TT'CG_AA
Cuts at: 2007 7679 2007
Size: 5672 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8875
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 4132 5553 2710
Size: 1422 1421 6032
Fragments arranged by size:
6032 1422 1421
BstYI r'GATC_y
Cuts at: 1972 2200 2371 3503 3723 4015 4189 5884 6205
Size: 228 171 1132 220 292 174 1695 321
Cuts at: 6205 6572 6957 6974 8426 8512 8524 8870 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1695 1452 1132 385 367 346 321
292 228 220 174 171 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4938 4938
Size: 8875
BtgI C'CryG_G
Cuts at: 513 1065 2465 513
Size: 552 1400 6923
Fragments arranged by size:
6923 1400 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8136
Fragments arranged by size:
8136 683 56
BtsI GCAGTG_nn'
Cuts at: 2504 3684 6391 7185 7205 2504
Size: 1180 2707 794 20 4174
Fragments arranged by size:
4174 2707 1180 794 20
ClaI AT'CG_AT
Cuts at: 1554 4390 1554
Size: 2836 6039
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5530 5565 529
Size: 35 4966 35 3839
Fragments arranged by size:
4966 3839 35 35
DraI TTT'AAA
Cuts at: 6179 6179
Size: 8875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3598 7882 809
Size: 507 2282 4284 1802
Fragments arranged by size:
4284 2282 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6322 7211 8840
Size: 54 1425 50 275 18 4492 889 1629
Cuts at: 8840 8
Size: 43
Fragments arranged by size:
4492 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6322 1537
Size: 275 4510 4090
Fragments arranged by size:
4510 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3843 4356 4449 4497 4533 4638 4747 4810
Size: 2490 513 93 48 36 105 109 63
Cuts at: 4810 4818 5764 5902 5982 6805 1353
Size: 8 946 138 80 823 3423
Fragments arranged by size:
3423 2490 946 823 513 138 109 105
93 80 63 48 36 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3323 7462 7836 7982 387
Size: 1064 138 1734 4139 374 146 1280
Fragments arranged by size:
4139 1734 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2346 3552 3809 4073 4738 4752
Size: 393 668 46 1206 257 264 665 14
Cuts at: 4752 5722 5785 5818 6938 7535 8322 8590 1239
Size: 970 63 33 1120 597 787 268 1524
Fragments arranged by size:
1524 1206 1120 970 787 668 665 597
393 268 264 257 63 46 33 14
EcoNI CCTnn'n_nnAGG
Cuts at: 5079 5079
Size: 8875
EcoO109I rG'GnC_Cy
Cuts at: 4727 4922 5225 5255 5285 5315 5345 5375 5434
Size: 195 303 30 30 30 30 30 59
Cuts at: 5434 5435 5686 5912 5995 4727
Size: 1 251 226 83 7607
Fragments arranged by size:
7607 303 251 226 195 83 59 30
30 30 30 30 1
EcoRI G'AATT_C
Cuts at: 2600 5403 6184 6562 2600
Size: 2803 781 378 4913
Fragments arranged by size:
4913 2803 781 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 3582 3782 2233
Size: 1349 200 7326
Fragments arranged by size:
7326 1349 200
FspI TGC'GCA
Cuts at: 7381 7381
Size: 8875
HaeII r_GCGC'y
Cuts at: 1678 2539 2653 3766 4161 5181 8022 1678
Size: 861 114 1113 395 1020 2841 2531
Fragments arranged by size:
2841 2531 1113 1020 861 395 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 2932 2964 3532
Size: 32 135 32 1112 32 216 32 568
Cuts at: 3532 3564 3715 3747 4790 4822 4937 4969 5177
Size: 32 151 32 1043 32 115 32 208
Cuts at: 5177 5180 5209 5212 5523 5555 5575 5607 5904
Size: 3 29 3 311 32 20 32 297
Cuts at: 5904 5936 6095 6127 7516 7548 7590 7622 1373
Size: 32 159 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1389 1112 1043 568 311 297 216
208 159 151 135 115 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 29 20 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2258 6219 6465 669
Size: 988 281 320 3961 246 3079
Fragments arranged by size:
3961 3079 988 320 281 246
HindIII A'AGCT_T
Cuts at: 748 2562 748
Size: 1814 7061
HpaI GTT'AAC
Cuts at: 6465 6465
Size: 8875
KpnI G_GTAC'C
Cuts at: 6200 6200
Size: 8875
MfeI C'AATT_G
Cuts at: 3196 3744 6474 3196
Size: 548 2730 5597
Fragments arranged by size:
5597 2730 548
MluI A'CGCG_T
Cuts at: 2588 8751 2588
Size: 6163 2712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3442 4590 5220 5340 7989 8173 1305
Size: 2137 1148 630 120 2649 184 2007
Fragments arranged by size:
2649 2137 2007 1148 630 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7053
Fragments arranged by size:
7053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5058 6833 7192 7351 518
Size: 648 3892 1775 359 159 2042
Fragments arranged by size:
3892 2042 1775 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2731 3589 3721 5117 5780 6291 6968 8116
Size: 1125 858 132 1396 663 511 677 1148
Cuts at: 8116 8361 1606
Size: 245 2120
Fragments arranged by size:
2120 1396 1148 1125 858 677 663 511
245 132
NaeI GCC'GGC
Cuts at: 2028 2028
Size: 8875
NcoI C'CATG_G
Cuts at: 513 1065 2465 513
Size: 552 1400 6923
Fragments arranged by size:
6923 1400 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8875
NgoMIV G'CCGG_C
Cuts at: 2026 2026
Size: 8875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8875
NotI GC'GGCC_GC
Cuts at: 6322 6322
Size: 8875
NsiI A_TGCA'T
Cuts at: 2709 2902 2709
Size: 193 8682
NspI r_CATG'y
Cuts at: 1132 1229 3565 6233 7778 1132
Size: 97 2336 2668 1545 2229
Fragments arranged by size:
2668 2336 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8875
PciI A'CATG_T
Cuts at: 7774 7774
Size: 8875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5141 1108
Size: 4033 4842
PmeI GTTT'AAAC
Cuts at: 6179 6179
Size: 8875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6944 6976 8457 8489 6944
Size: 32 1481 32 7330
Fragments arranged by size:
7330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4727 5225 5255 5285 5315 5345 5375 5686 5912
Size: 498 30 30 30 30 30 311 226
Cuts at: 5912 5995 4727
Size: 83 7607
Fragments arranged by size:
7607 498 311 226 83 30 30 30
30 30
PshAI GACnn'nnGTC
Cuts at: 1613 5223 5283 5313 5343 5808 1613
Size: 3610 60 30 30 465 4680
Fragments arranged by size:
4680 3610 465 60 30 30
PsiI TTA'TAA
Cuts at: 6445 6445
Size: 8875
PspOMI G'GGCC_C
Cuts at: 5434 5434
Size: 8875
PstI C_TGCA'G
Cuts at: 830 3345 6227 830
Size: 2515 2882 3478
Fragments arranged by size:
3478 2882 2515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8875
PvuII CAG'CTG
Cuts at: 2731 3589 5117 5780 2731
Size: 858 1528 663 5826
Fragments arranged by size:
5826 1528 858 663
SalI G'TCGA_C
Cuts at: 1936 6217 1936
Size: 4281 4594
SanDI GG'GwC_CC
Cuts at: 5225 5255 5285 5315 5345 5995 5225
Size: 30 30 30 30 650 8105
Fragments arranged by size:
8105 650 30 30 30 30
SapI GCTCTTCn'nnn_
Cuts at: 4356 4533 4818 5982 4356
Size: 177 285 1164 7249
Fragments arranged by size:
7249 1164 285 177
SbfI CC_TGCA'GG
Cuts at: 6227 6227
Size: 8875
ScaI AGT'ACT
Cuts at: 3609 7123 8835 3609
Size: 3514 1712 3649
Fragments arranged by size:
3649 3514 1712
SexAI A'CCwGG_T
Cuts at: 5518 5518
Size: 8875
SfcI C'TryA_G
Cuts at: 826 1045 3341 4441 6223 7358 8039 8230 826
Size: 219 2296 1100 1782 1135 681 191 1471
Fragments arranged by size:
2296 1782 1471 1135 1100 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8875
SmaI CCC'GGG
Cuts at: 1872 6202 1872
Size: 4330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 3525 3767 4004 5512 5598
Size: 197 926 135 1447 242 237 1508 86
Cuts at: 5598 5806 6979 7880 8142 820
Size: 208 1173 901 262 1553
Fragments arranged by size:
1553 1508 1447 1173 926 901 262 242
237 208 197 135 86
SnaBI TAC'GTA
Cuts at: 493 6172 6666 493
Size: 5679 494 2702
Fragments arranged by size:
5679 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8875
SphI G_CATG'C
Cuts at: 1132 3565 6233 1132
Size: 2433 2668 3774
Fragments arranged by size:
3774 2668 2433
SspI AAT'ATT
Cuts at: 5 52 2383 3781 6799 5
Size: 47 2331 1398 3018 2081
Fragments arranged by size:
3018 2331 2081 1398 47
StuI AGG'CCT
Cuts at: 1517 5011 6083 1517
Size: 3494 1072 4309
Fragments arranged by size:
4309 3494 1072
StyI C'CwwG_G
Cuts at: 513 1065 2465 5378 5438 5525 5747 6314 513
Size: 552 1400 2913 60 87 222 567 3074
Fragments arranged by size:
3074 2913 1400 567 552 222 87 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3956 5919 6051 6063 6069 6902 6919
Size: 1255 2376 1963 132 12 6 833 17
Cuts at: 6919 7072 7257 7702 325
Size: 153 185 445 1498
Fragments arranged by size:
2376 1963 1498 1255 833 445 185 153
132 17 12 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3157 3607 7121
Size: 274 80 33 51 1443 1180 450 3514
Cuts at: 7121 8833 96
Size: 1712 138
Fragments arranged by size:
3514 1712 1443 1180 450 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8835 8867 8835
Size: 32 8843
Tth111I GACn'n_nGTC
Cuts at: 1316 4932 5520 1316
Size: 3616 588 4671
Fragments arranged by size:
4671 3616 588
XbaI T'CTAG_A
Cuts at: 2374 2525 2930 4673 6211 2374
Size: 151 405 1743 1538 5038
Fragments arranged by size:
5038 1743 1538 405 151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4770 5236 5266 5296 5326 5356 4770
Size: 466 30 30 30 30 8289
Fragments arranged by size:
8289 466 30 30 30 30
XhoI C'TCGA_G
Cuts at: 1943 3525 5598 1943
Size: 1582 2073 5220
Fragments arranged by size:
5220 2073 1582
XmaI C'CCGG_G
Cuts at: 1870 6200 1870
Size: 4330 4545
XmnI GAAnn'nnTTC
Cuts at: 2521 2787 4140 7004 2521
Size: 266 1353 2864 4392
Fragments arranged by size:
4392 2864 1353 266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6683 276
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SfiI SfoI SrfI SwaI