Restriction map for
pFN21ASDA0135
(Circular) (Six-base) MAPSORT of: pFN21ASDA0135.seq Check: 6286 from: 1 to: 8719
pFN21ASDA0135 8719 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3255 6529 278
Size: 53 83 186 936 1719 3274 2468
Fragments arranged by size:
3274 2468 1719 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4744 6062 1937
Size: 2807 1318 4594
Fragments arranged by size:
4594 2807 1318
Acc65I G'GTAC_C
Cuts at: 2939 6040 2939
Size: 3101 5618
AclI AA'CG_TT
Cuts at: 1563 6846 7219 1563
Size: 5283 373 3063
Fragments arranged by size:
5283 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2238 3690 6782 8166 8434 1632
Size: 606 1452 3092 1384 268 1917
Fragments arranged by size:
3092 1917 1452 1384 606 268
AfeI AGC'GCT
Cuts at: 2211 2211
Size: 8719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8522
AflIII A'CryG_T
Cuts at: 1203 2811 4558 4593 7618 8595 1203
Size: 1608 1747 35 3025 977 1327
Fragments arranged by size:
3025 1747 1608 1327 977 35
AgeI A'CCGG_T
Cuts at: 2999 7550 2999
Size: 4551 4168
AhdI GACnn_n'nnGTC
Cuts at: 4285 7448 4285
Size: 3163 5556
AleI CACnn'nnGTG
Cuts at: 1166 2621 4646 1166
Size: 1455 2025 5239
Fragments arranged by size:
5239 2025 1455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2307 3573 1184
Size: 1123 1266 6330
Fragments arranged by size:
6330 1266 1123
AlwNI CAG_nnn'CTG
Cuts at: 2193 2506 2549 2663 3535 4944 5750 6462 8034
Size: 313 43 114 872 1409 806 712 1572
Cuts at: 8034 2193
Size: 2878
Fragments arranged by size:
2878 1572 1409 872 806 712 313 114
43
ApaI G_GGCC'C
Cuts at: 2108 4781 5945 2108
Size: 2673 1164 4882
Fragments arranged by size:
4882 2673 1164
ApaLI G'TGCA_C
Cuts at: 3518 5550 6776 7932 3518
Size: 2032 1226 1156 4305
Fragments arranged by size:
4305 2032 1226 1156
ApoI r'AATT_y
Cuts at: 1493 4231 6028 6254 6406 8515 1493
Size: 2738 1797 226 152 2109 1697
Fragments arranged by size:
2738 2109 1797 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3339 7273 160
Size: 872 2307 3934 1606
Fragments arranged by size:
3934 2307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
AvaI C'yCGr_G
Cuts at: 1870 1943 3372 5987 6044 1870
Size: 73 1429 2615 57 4545
Fragments arranged by size:
4545 2615 1429 73 57
BamHI G'GATC_C
Cuts at: 3328 5149 5992 6049 6416 3328
Size: 1821 843 57 367 5631
Fragments arranged by size:
5631 1821 843 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2765 2939 4427 4697 6040
Size: 325 217 627 978 174 1488 270 1343
Cuts at: 6040 7495 618
Size: 1455 1842
Fragments arranged by size:
1842 1488 1455 1343 978 627 325 270
217 174
BanII G_rGCy'C
Cuts at: 1391 2108 2133 2234 2484 2724 3150 3596 4045
Size: 717 25 101 250 240 426 446 449
Cuts at: 4045 4352 4773 4781 4905 4959 5033 5331 5945
Size: 307 421 8 124 54 74 298 614
Cuts at: 5945 6038 1391
Size: 93 4072
Fragments arranged by size:
4072 717 614 449 446 426 421 307
298 250 240 124 101 93 74 54
25 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 928
Size: 971 327 7421
Fragments arranged by size:
7421 971 327
BbvCI CC'TCA_GC
Cuts at: 2149 2567 3315 3471 3687 4159 5454 2149
Size: 418 748 156 216 472 1295 5414
Fragments arranged by size:
5414 1295 748 472 418 216 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3912 3946 5531 5565 6910 6944 1903
Size: 34 1975 34 1585 34 1345 34 3678
Fragments arranged by size:
3678 1975 1585 1345 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5387 6611 7821 5387
Size: 1224 1210 6285
Fragments arranged by size:
6285 1224 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4875 5037 7330
Size: 107 122 71 631 605 3203 162 2293
Cuts at: 7330 136
Size: 1525
Fragments arranged by size:
3203 2293 1525 631 605 162 122 107
71
BglII A'GATC_T
Cuts at: 8714 8714
Size: 8719
BlpI GC'TnA_GC
Cuts at: 2127 4899 6136 2127
Size: 2772 1237 4710
Fragments arranged by size:
4710 2772 1237
Bme1580I G_kGCm'C
Cuts at: 1790 2108 3372 3522 4601 4781 5554 5945 6780
Size: 318 1264 150 1079 180 773 391 835
Cuts at: 6780 7936 1790
Size: 1156 2573
Fragments arranged by size:
2573 1264 1156 1079 835 773 391 318
180 150
BmgBI CAC'GTC
Cuts at: 1318 2775 2827 1318
Size: 1457 52 7210
Fragments arranged by size:
7210 1457 52
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4018 6450 7408
Size: 416 53 83 399 24 2601 2432 958
Cuts at: 7408 442
Size: 1753
Fragments arranged by size:
2601 2432 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 3467 4348 2752
Size: 715 881 7123
Fragments arranged by size:
7123 881 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 3462 3465 3660 4358 4757 4997 5615
Size: 1499 724 3 195 698 399 240 618
Cuts at: 5615 5681 5906 7379 1239
Size: 66 225 1473 2579
Fragments arranged by size:
2579 1499 1473 724 698 618 399 240
225 195 66 3
Bpu10I CC'TnA_GC
Cuts at: 2149 2165 2301 2567 3315 3471 3687 4159 5454
Size: 16 136 266 748 156 216 472 1295
Cuts at: 5454 5925 2149
Size: 471 4943
Fragments arranged by size:
4943 1295 748 472 471 266 216 156
136 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2888 4235 4520 6844 7709 8007 2888
Size: 1347 285 2324 865 298 3600
Fragments arranged by size:
3600 2324 1347 865 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2740 4122 5610 5660 7382 882
Size: 617 1241 1382 1488 50 1722 2219
Fragments arranged by size:
2219 1722 1488 1382 1241 617 50
BsaAI yAC'GTr
Cuts at: 493 1204 2812 4596 6016 6510 493
Size: 711 1608 1784 1420 494 2702
Fragments arranged by size:
2702 1784 1608 1420 711 494
BsaBI GATnn'nnATC
Cuts at: 8713 8713
Size: 8719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3252 6526 6908 7543
Size: 53 83 186 936 1719 3274 382 635
Cuts at: 7543 275
Size: 1451
Fragments arranged by size:
3274 1719 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2999 3384 4907 5554 7152 7550 7824 7971 8382
Size: 385 1523 647 1598 398 274 147 411
Cuts at: 8382 2999
Size: 3336
Fragments arranged by size:
3336 1598 1523 647 411 398 385 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4004 4034 5588 5618 5893
Size: 30 165 30 2601 30 1554 30 275
Cuts at: 5893 5923 1178
Size: 30 3974
Fragments arranged by size:
3974 2601 1554 275 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 3002 4147 4625 4808 4967 5654 5678
Size: 1402 414 1145 478 183 159 687 24
Cuts at: 5678 1186
Size: 4227
Fragments arranged by size:
4227 1402 1145 687 478 414 183 159
24
BseYI C'CCAG_C
Cuts at: 2107 2132 2831 2978 3310 3682 3735 3764 3785
Size: 25 699 147 332 372 53 29 21
Cuts at: 3785 3905 3964 4913 5035 7922 8418 2107
Size: 120 59 949 122 2887 496 2408
Fragments arranged by size:
2887 2408 949 699 496 372 332 147
122 120 59 53 29 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2097 2267 2885 3034 4129 1163
Size: 573 361 170 618 149 1095 5753
Fragments arranged by size:
5753 1095 618 573 361 170 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 5308 5493 6169 6930 7958
Size: 275 186 1981 1326 185 676 761 1028
Cuts at: 7958 1540
Size: 2301
Fragments arranged by size:
2301 1981 1326 1028 761 676 275 186
185
BsiHKAI G_wGCw'C
Cuts at: 2234 2307 2580 3150 3522 4773 4905 4932 5205
Size: 73 273 570 372 1251 132 27 273
Cuts at: 5205 5554 6780 6865 7936 2234
Size: 349 1226 85 1071 3017
Fragments arranged by size:
3017 1251 1226 1071 570 372 349 273
273 132 85 73 27
BsmI GAATG_Cn'
Cuts at: 6230 6323 6230
Size: 93 8626
BsmBI CGTCTCn'nnnn_
Cuts at: 4719 5749 4719
Size: 1030 7689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2133 2234 2307 2484 2580 2724
Size: 399 318 25 101 73 177 96 144
Cuts at: 2724 3150 3372 3522 3596 4045 4352 4601 4773
Size: 426 222 150 74 449 307 249 172
Cuts at: 4773 4781 4905 4932 4959 5033 5205 5331 5554
Size: 8 124 27 27 74 172 126 223
Cuts at: 5554 5945 6038 6780 6865 7936 1391
Size: 391 93 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 426 399 391 318
307 249 223 222 177 172 172 150
144 126 124 101 96 93 85 74
74 73 27 27 25 8
BspEI T'CCGG_A
Cuts at: 3384 8382 3384
Size: 4998 3721
BspHI T'CATG_A
Cuts at: 5715 6608 8338 5715
Size: 893 1730 6096
Fragments arranged by size:
6096 1730 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 3612 4007 5406 6074 844
Size: 1283 1485 395 1399 668 3489
Fragments arranged by size:
3489 1485 1399 1283 668 395
BsrBI CCG'CTC
Cuts at: 1394 1823 3021 3745 4097 6606 1394
Size: 429 1198 724 352 2509 3507
Fragments arranged by size:
3507 2509 1198 724 429 352
BsrDI GCAATG_nn'
Cuts at: 65 1248 3265 7214 7388 65
Size: 1183 2017 3949 174 1396
Fragments arranged by size:
3949 2017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2999 4424 7363 7550 1700
Size: 110 1189 1425 2939 187 2869
Fragments arranged by size:
2939 2869 1425 1189 187 110
BsrGI T'GTAC_A
Cuts at: 96 2270 5622 96
Size: 2174 3352 3193
Fragments arranged by size:
3352 3193 2174
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8719
BssSI C'ACGA_G
Cuts at: 6779 7791 6779
Size: 1012 7707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8719
BstBI TT'CG_AA
Cuts at: 2007 7523 2007
Size: 5516 3203
BstEII G'GTnAC_C
Cuts at: 1184 4633 1184
Size: 3449 5270
BstXI CCAn_nnnn'nTGG
Cuts at: 2762 3247 4921 4969 2762
Size: 485 1674 48 6512
Fragments arranged by size:
6512 1674 485 48
BstYI r'GATC_y
Cuts at: 1972 2451 3328 3381 3604 4686 5149 5992 6049
Size: 479 877 53 223 1082 463 843 57
Cuts at: 6049 6416 6801 6818 8270 8356 8368 8714 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1082 877 843 479 463 385
367 346 223 86 57 53 17 12
BstZ17I GTA'TAC
Cuts at: 4745 4745
Size: 8719
Bsu36I CC'TnA_GG
Cuts at: 2368 4587 2368
Size: 2219 6500
BtgI C'CryG_G
Cuts at: 513 1065 2022 2324 2724 3094 3293 3795 3959
Size: 552 957 302 400 370 199 502 164
Cuts at: 3959 4179 5670 5789 513
Size: 220 1491 119 3443
Fragments arranged by size:
3443 1491 957 552 502 400 370 302
220 199 164 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2775 487
Size: 683 56 1549 6431
Fragments arranged by size:
6431 1549 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 2284 2338 3555 3706 3847 4212 4350 5047
Size: 163 54 1217 151 141 365 138 697
Cuts at: 5047 5394 6235 7029 7049 2121
Size: 347 841 794 20 3791
Fragments arranged by size:
3791 1217 841 794 697 365 347 163
151 141 138 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3247 3282 529
Size: 35 2683 35 5966
Fragments arranged by size:
5966 2683 35 35
DraI TTT'AAA
Cuts at: 6023 6023
Size: 8719
DraIII CAC_nnn'GTG
Cuts at: 3367 3367
Size: 8719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7726 809
Size: 507 6410 1802
Fragments arranged by size:
6410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2512 2593 2881
Size: 54 1425 50 275 18 682 81 288
Cuts at: 2881 3237 3358 3798 4176 4444 5490 5968 6166
Size: 356 121 440 378 268 1046 478 198
Cuts at: 6166 7055 8684 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1046 889 682 478 440 378
356 288 275 268 198 121 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5490 6166 1537
Size: 275 3678 676 4090
Fragments arranged by size:
4090 3678 676 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 2669 2920 3981 4339 5297 5826 6649
Size: 693 623 251 1061 358 958 529 823
Cuts at: 6649 1353
Size: 3423
Fragments arranged by size:
3423 1061 958 823 693 623 529 358
251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7306 7680 7826 387
Size: 1064 138 5717 374 146 1280
Fragments arranged by size:
5717 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2232 3148 4771 4903 2232
Size: 916 1623 132 6048
Fragments arranged by size:
6048 1623 916 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2238 2738 3462 3465 3660 3690 4358
Size: 393 606 500 724 3 195 30 668
Cuts at: 4358 4757 4997 5615 5681 5906 6782 7379 8166
Size: 399 240 618 66 225 876 597 787
Cuts at: 8166 8434 1239
Size: 268 1524
Fragments arranged by size:
1524 876 787 724 668 618 606 597
500 399 393 268 240 225 195 66
30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 3480 2570
Size: 910 7809
EcoO109I rG'GnC_Cy
Cuts at: 2104 2503 2563 3307 4195 4777 4778 4848 5046
Size: 399 60 744 888 582 1 70 198
Cuts at: 5046 5941 5942 2104
Size: 895 1 4881
Fragments arranged by size:
4881 895 888 744 582 399 198 70
60 1 1
EcoRI G'AATT_C
Cuts at: 4231 6028 6406 4231
Size: 1797 378 6544
Fragments arranged by size:
6544 1797 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 4276 2440
Size: 1836 6883
FspI TGC'GCA
Cuts at: 7225 7225
Size: 8719
HaeII r_GCGC'y
Cuts at: 1678 2213 2695 3536 7866 1678
Size: 535 482 841 4330 2531
Fragments arranged by size:
4330 2531 841 535 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3366 3398 3931 3963 4004
Size: 32 135 32 1794 32 533 32 41
Cuts at: 4004 4036 4271 4303 5134 5166 5471 5503 5588
Size: 32 235 32 831 32 305 32 85
Cuts at: 5588 5590 5620 5622 7360 7392 7434 7466 1373
Size: 2 30 2 1738 32 42 32 2626
Fragments arranged by size:
2626 1794 1738 831 533 305 235 135
85 42 41 32 32 32 32 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4438 6063 6309 669
Size: 988 281 2500 1625 246 3079
Fragments arranged by size:
3079 2500 1625 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2472 748
Size: 1724 6995
HpaI GTT'AAC
Cuts at: 6309 6309
Size: 8719
KpnI G_GTAC'C
Cuts at: 2943 6044 2943
Size: 3101 5618
MfeI C'AATT_G
Cuts at: 6318 6318
Size: 8719
MluI A'CGCG_T
Cuts at: 8595 8595
Size: 8719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2902 4097 4511 4554 5120 5374 7833 8017
Size: 1597 1195 414 43 566 254 2459 184
Cuts at: 8017 1305
Size: 2007
Fragments arranged by size:
2459 2007 1597 1195 566 414 254 184
43
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2514 2883 3239 3360 3800
Size: 54 1425 343 682 369 356 121 440
Cuts at: 3800 4178 4446 5970 10
Size: 378 268 1524 2759
Fragments arranged by size:
2759 1524 1425 682 440 378 369 356
343 268 121 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 4646 6677 7036 7195 518
Size: 648 1455 2025 2031 359 159 2042
Fragments arranged by size:
2042 2031 2025 1455 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2111 2326 3243 3314 3686 3768 3823 4018
Size: 505 215 917 71 372 82 55 195
Cuts at: 4018 4681 4895 5035 5876 6135 6812 7960 8205
Size: 663 214 140 841 259 677 1148 245
Cuts at: 8205 1606
Size: 2120
Fragments arranged by size:
2120 1148 917 841 677 663 505 372
259 245 215 214 195 140 82 71
55
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 7210
Fragments arranged by size:
7210 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8719
NotI GC'GGCC_GC
Cuts at: 6166 6166
Size: 8719
NspI r_CATG'y
Cuts at: 1132 1229 3715 6077 7622 1132
Size: 97 2486 2362 1545 2229
Fragments arranged by size:
2486 2362 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4190 1348
Size: 2842 5877
PciI A'CATG_T
Cuts at: 7618 7618
Size: 8719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4944 5861 1108
Size: 3836 917 3966
Fragments arranged by size:
3966 3836 917
PfoI T'CCnGG_A
Cuts at: 2715 4241 4374 4395 4461 4689 4706 2715
Size: 1526 133 21 66 228 17 6728
Fragments arranged by size:
6728 1526 228 133 66 21 17
PmeI GTTT'AAAC
Cuts at: 6023 6023
Size: 8719
PmlI CAC'GTG
Cuts at: 4596 4596
Size: 8719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6788 6820 8301 8333 6788
Size: 32 1481 32 7174
Fragments arranged by size:
7174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 3307 4848 2503
Size: 804 1541 6374
Fragments arranged by size:
6374 1541 804
PshAI GACnn'nnGTC
Cuts at: 1613 2744 1613
Size: 1131 7588
PsiI TTA'TAA
Cuts at: 6289 6289
Size: 8719
PspOMI G'GGCC_C
Cuts at: 2104 4777 5941 2104
Size: 2673 1164 4882
Fragments arranged by size:
4882 2673 1164
PstI C_TGCA'G
Cuts at: 830 3996 4680 5742 6071 830
Size: 3166 684 1062 329 3478
Fragments arranged by size:
3478 3166 1062 684 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8719
PvuII CAG'CTG
Cuts at: 2111 3243 3314 3686 3823 4018 2111
Size: 1132 71 372 137 195 6812
Fragments arranged by size:
6812 1132 372 195 137 71
SacI G_AGCT'C
Cuts at: 2234 3150 4773 4905 2234
Size: 916 1623 132 6048
Fragments arranged by size:
6048 1623 916 132
SacII CC_GC'GG
Cuts at: 2327 2327
Size: 8719
SalI G'TCGA_C
Cuts at: 1936 6061 1936
Size: 4125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2920 3981 2920
Size: 1061 7658
SbfI CC_TGCA'GG
Cuts at: 6071 6071
Size: 8719
ScaI AGT'ACT
Cuts at: 5006 5546 6967 8679 5006
Size: 540 1421 1712 5046
Fragments arranged by size:
5046 1712 1421 540
SexAI A'CCwGG_T
Cuts at: 5697 5697
Size: 8719
SfcI C'TryA_G
Cuts at: 826 1045 3187 3992 4676 5578 5738 6067 7202
Size: 219 2142 805 684 902 160 329 1135
Cuts at: 7202 7883 8074 826
Size: 681 191 1471
Fragments arranged by size:
2142 1471 1135 902 805 684 681 329
219 191 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
SgrAI Cr'CCGG_yG
Cuts at: 1700 2999 1700
Size: 1299 7420
SmaI CCC'GGG
Cuts at: 1872 5989 6046 1872
Size: 4117 57 4545
Fragments arranged by size:
4545 4117 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 4250 4499 6823 7724 7986
Size: 197 926 960 1347 249 2324 901 262
Cuts at: 7986 820
Size: 1553
Fragments arranged by size:
2324 1553 1347 960 926 901 262 249
197
SnaBI TAC'GTA
Cuts at: 493 6016 6510 493
Size: 5523 494 2702
Fragments arranged by size:
5523 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8719
SphI G_CATG'C
Cuts at: 1132 6077 1132
Size: 4945 3774
SspI AAT'ATT
Cuts at: 5 52 2266 6643 5
Size: 47 2214 4377 2081
Fragments arranged by size:
4377 2214 2081 47
StuI AGG'CCT
Cuts at: 1517 3927 1517
Size: 2410 6309
StyI C'CwwG_G
Cuts at: 513 1065 2022 3285 3332 3540 3561 3813 4173
Size: 552 957 1263 47 208 21 252 360
Cuts at: 4173 4338 4441 4447 4616 4962 5242 5766 5971
Size: 165 103 6 169 346 280 524 205
Cuts at: 5971 6158 513
Size: 187 3074
Fragments arranged by size:
3074 1263 957 552 524 360 346 280
252 208 205 187 169 165 103 47
21 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3031 3258 3996 4898 6746 6763 6916
Size: 1255 1451 227 738 902 1848 17 153
Cuts at: 6916 7101 7546 325
Size: 185 445 1498
Fragments arranged by size:
1848 1498 1451 1255 902 738 445 227
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2270 5004 5016
Size: 274 80 33 51 1443 293 2734 12
Cuts at: 5016 5544 5622 6965 8677 96
Size: 528 78 1343 1712 138
Fragments arranged by size:
2734 1712 1443 1343 528 293 274 138
80 78 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4550 4582 5822 5854 5893 5925 8679 8711 4550
Size: 32 1240 32 39 32 2754 32 4558
Fragments arranged by size:
4558 2754 1240 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3494 1316
Size: 2178 6541
XbaI T'CTAG_A
Cuts at: 3949 6055 3949
Size: 2106 6613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2507 2664 3820 5032 2428
Size: 79 157 1156 1212 6115
Fragments arranged by size:
6115 1212 1156 157 79
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8719
XmaI C'CCGG_G
Cuts at: 1870 5987 6044 1870
Size: 4117 57 4545
Fragments arranged by size:
4545 4117 57
XmnI GAAnn'nnTTC
Cuts at: 4068 5648 6848 4068
Size: 1580 1200 5939
Fragments arranged by size:
5939 1580 1200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3253 6527 276
Size: 53 83 186 936 1719 3274 2468
Fragments arranged by size:
3274 2468 1719 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SanDI SfiI SfoI
SrfI SwaI