Restriction map for
pFN21ASDA0132
(Circular) (Six-base) MAPSORT of: pFN21ASDA0132.seq Check: 3728 from: 1 to: 6601
pFN21ASDA0132 6601 bp
With 182 enzymes: SgfI *
April 10, 2017 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2956 3088 2956
Size: 132 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2259 4411 278
Size: 53 83 186 936 723 2152 2468
Fragments arranged by size:
2468 2152 936 723 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3944 1937
Size: 2007 4594
Acc65I G'GTAC_C
Cuts at: 3922 3922
Size: 6601
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2161 4664 6048 6316 1632
Size: 529 2503 1384 268 1917
Fragments arranged by size:
2503 1917 1384 529 268
AfeI AGC'GCT
Cuts at: 3629 3629
Size: 6601
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 3747 5500 6477 1203
Size: 2544 1753 977 1327
Fragments arranged by size:
2544 1753 1327 977
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 5330 5330
Size: 6601
AleI CACnn'nnGTG
Cuts at: 1166 2625 2966 3336 1166
Size: 1459 341 370 4431
Fragments arranged by size:
4431 1459 370 341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2643 3574 1184
Size: 1459 931 4211
Fragments arranged by size:
4211 1459 931
AlwNI CAG_nnn'CTG
Cuts at: 2248 2557 2821 4344 5916 2248
Size: 309 264 1523 1572 2933
Fragments arranged by size:
2933 1572 1523 309 264
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2435 3910 4136 4288 6397 1493
Size: 942 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 942 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 3926 1870
Size: 73 688 1295 4545
Fragments arranged by size:
4545 1295 688 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3559 3592 3559
Size: 33 6568
BamHI G'GATC_C
Cuts at: 3931 4298 3931
Size: 367 6234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 3071 3100 3922 5377
Size: 325 217 627 1196 88 29 822 1455
Cuts at: 5377 618
Size: 1842
Fragments arranged by size:
1842 1455 1196 822 627 325 217 88
29
BanII G_rGCy'C
Cuts at: 1391 2946 3858 3920 1391
Size: 1555 912 62 4072
Fragments arranged by size:
4072 1555 912 62
BbeI G_GCGC'C
Cuts at: 2987 2987
Size: 6601
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 2749 928
Size: 971 433 417 4780
Fragments arranged by size:
4780 971 433 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2557 2591 2845 2879 4792 4826 1903
Size: 34 620 34 254 34 1913 34 3678
Fragments arranged by size:
3678 1913 620 254 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2414 4493 5703 2414
Size: 2079 1210 3312
Fragments arranged by size:
3312 2079 1210
BclI T'GATC_A
Cuts at: 1548 3519 1548
Size: 1971 4630
BfrBI ATG'CAT
Cuts at: 2650 2650
Size: 6601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2447 3120 5212
Size: 107 122 71 631 605 775 673 2092
Cuts at: 5212 136
Size: 1525
Fragments arranged by size:
2092 1525 775 673 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2931 6596 2931
Size: 3665 2936
BlpI GC'TnA_GC
Cuts at: 2237 2595 3038 4018 2237
Size: 358 443 980 4820
Fragments arranged by size:
4820 980 443 358
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2632 3258 3691 4662 5818 1790
Size: 303 539 626 433 971 1156 2573
Fragments arranged by size:
2573 1156 971 626 539 433 303
BmgBI CAC'GTC
Cuts at: 1318 2485 3651 1318
Size: 1167 1166 4268
Fragments arranged by size:
4268 1167 1166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2785 3057
Size: 416 53 83 399 24 873 495 272
Cuts at: 3057 4332 5290 442
Size: 1275 958 1753
Fragments arranged by size:
1753 1275 958 873 495 416 399 272
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 3068 3175 3814 5261 1239
Size: 971 858 107 639 1447 2579
Fragments arranged by size:
2579 1447 971 858 639 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4726 5591 5889 4726
Size: 865 298 5438
Fragments arranged by size:
5438 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5264 882
Size: 617 3765 2219
Fragments arranged by size:
3765 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3898 4392 493
Size: 711 2694 494 2702
Fragments arranged by size:
2702 2694 711 494
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2150 2256 2852 2984
Size: 53 83 186 936 617 106 596 132
Cuts at: 2984 3555 4408 4790 5425 275
Size: 571 853 382 635 1451
Fragments arranged by size:
1451 936 853 635 617 596 571 382
186 132 106 83 53
BsaWI w'CCGG_w
Cuts at: 3081 5034 5432 5706 5853 6264 3081
Size: 1953 398 274 147 411 3418
Fragments arranged by size:
3418 1953 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 2435 2465 3173
Size: 30 165 30 972 30 30 30 708
Cuts at: 3173 3203 1178
Size: 30 4576
Fragments arranged by size:
4576 972 708 165 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 2954 1186
Size: 1504 264 4833
Fragments arranged by size:
4833 1504 264
BseYI C'CCAG_C
Cuts at: 2051 2157 2564 3587 5804 6300 2051
Size: 106 407 1023 2217 496 2352
Fragments arranged by size:
2352 2217 1023 496 407 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 2936 3551 1163
Size: 573 834 366 615 4213
Fragments arranged by size:
4213 834 615 573 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2292 4051 4812 5840 1540
Size: 275 186 291 1759 761 1028 2301
Fragments arranged by size:
2301 1759 1028 761 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2946 4662 4747 5818 2946
Size: 1716 85 1071 3729
Fragments arranged by size:
3729 1716 1071 85
BsmI GAATG_Cn'
Cuts at: 4112 4205 4112
Size: 93 6508
BsmBI CGTCTCn'nnnn_
Cuts at: 3641 3641
Size: 6601
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2632 2946 3258 3691 3858 3920
Size: 399 303 539 314 312 433 167 62
Cuts at: 3920 4662 4747 5818 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 539 433 399 314 312
303 167 85 62
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 2220 2487 4490 6220 2220
Size: 267 2003 1730 2601
Fragments arranged by size:
2601 2003 1730 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2257 2956 3088 3102 3956 844
Size: 1413 699 132 14 854 3489
Fragments arranged by size:
3489 1413 854 699 132 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 3361 4488 1394
Size: 429 1288 250 1127 3507
Fragments arranged by size:
3507 1288 1127 429 250
BsrDI GCAATG_nn'
Cuts at: 65 1248 2575 3534 5096 5270 65
Size: 1183 1327 959 1562 174 1396
Fragments arranged by size:
1562 1396 1327 1183 959 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2287 3119 3678 5245 5432 1700
Size: 110 477 832 559 1567 187 2869
Fragments arranged by size:
2869 1567 832 559 477 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 4661 5673 4661
Size: 1012 5589
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2971 794
Size: 2177 4424
BstBI TT'CG_AA
Cuts at: 2007 5405 2007
Size: 3398 3203
BstEII G'GTnAC_C
Cuts at: 1184 2707 3802 1184
Size: 1523 1095 3983
Fragments arranged by size:
3983 1523 1095
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2461 3199 2419
Size: 42 738 5821
Fragments arranged by size:
5821 738 42
BstYI r'GATC_y
Cuts at: 1972 2032 2889 2931 3931 4298 4683 4700 6152
Size: 60 857 42 1000 367 385 17 1452
Cuts at: 6152 6238 6250 6596 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1000 857 385 367 346 86
60 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2094 2094
Size: 6601
BtgI C'CryG_G
Cuts at: 513 1065 2460 3012 3104 3150 3316 3444 3849
Size: 552 1395 552 92 46 166 128 405
Cuts at: 3849 513
Size: 3265
Fragments arranged by size:
3265 1395 552 552 405 166 128 92
46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2566 487
Size: 683 56 1340 4522
Fragments arranged by size:
4522 1340 683 56
BtsI GCAGTG_nn'
Cuts at: 2261 2542 2695 4117 4911 4931 2261
Size: 281 153 1422 794 20 3931
Fragments arranged by size:
3931 1422 794 281 153 20
ClaI AT'CG_AT
Cuts at: 1554 3285 1554
Size: 1731 4870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 3905 3905
Size: 6601
DraIII CAC_nnn'GTG
Cuts at: 2133 2314 3379 2133
Size: 181 1065 5355
Fragments arranged by size:
5355 1065 181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 2924 5608 809
Size: 507 1206 402 2684 1802
Fragments arranged by size:
2684 1802 1206 507 402
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2664 3117
Size: 54 1425 50 275 18 459 375 453
Cuts at: 3117 3289 4048 4937 6566 8
Size: 172 759 889 1629 43
Fragments arranged by size:
1629 1425 889 759 459 453 375 275
172 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2289 4048 1537
Size: 275 477 1759 4090
Fragments arranged by size:
4090 1759 477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4531 1353
Size: 3178 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2156 3104 5188 5562 5708 387
Size: 1064 138 567 948 2084 374 146 1280
Fragments arranged by size:
2084 1280 1064 948 567 374 146 138
EcoICRI GAG'CTC
Cuts at: 2944 2944
Size: 6601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 2210 3068 3175 3814 4664 5261
Size: 393 529 49 858 107 639 850 597
Cuts at: 5261 6048 6316 1239
Size: 787 268 1524
Fragments arranged by size:
1524 858 850 787 639 597 529 393
268 107 49
EcoO109I rG'GnC_Cy
Cuts at: 2055 2055
Size: 6601
EcoRI G'AATT_C
Cuts at: 2435 3910 4288 2435
Size: 1475 378 4748
Fragments arranged by size:
4748 1475 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 6601
FspI TGC'GCA
Cuts at: 5107 5107
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 2428 2499 2822 2987 3198 3631 5748 1678
Size: 750 71 323 165 211 433 2117 2531
Fragments arranged by size:
2531 2117 750 433 323 211 165 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3021 3053 5242
Size: 32 135 32 803 32 614 32 2189
Cuts at: 5242 5274 5316 5348 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2189 803 614 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3945 4191 669
Size: 988 281 912 1095 246 3079
Fragments arranged by size:
3079 1095 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6601
HpaI GTT'AAC
Cuts at: 4191 4191
Size: 6601
KasI G'GCGC_C
Cuts at: 2983 2983
Size: 6601
KpnI G_GTAC'C
Cuts at: 3926 3926
Size: 6601
MfeI C'AATT_G
Cuts at: 4200 4200
Size: 6601
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2925 3052 5715 5899 1305
Size: 1620 127 2663 184 2007
Fragments arranged by size:
2663 2007 1620 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 3291 10
Size: 54 1425 343 834 625 3320
Fragments arranged by size:
3320 1425 834 625 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2459 2625 2645 2966 3251 3272
Size: 648 1218 75 166 20 321 285 21
Cuts at: 3272 3297 3336 3527 4559 4918 5077 518
Size: 25 39 191 1032 359 159 2042
Fragments arranged by size:
2042 1218 1032 648 359 321 285 191
166 159 75 39 25 21 20
MspA1I CmG'CkG
Cuts at: 1606 2248 2557 2875 2910 3014 3106 3482 3613
Size: 642 309 318 35 104 92 376 131
Cuts at: 3613 4017 4694 5842 6087 1606
Size: 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 404 376 318 309
245 131 104 92 35
NaeI GCC'GGC
Cuts at: 3121 3121
Size: 6601
NarI GG'CG_CC
Cuts at: 2984 2984
Size: 6601
NcoI C'CATG_G
Cuts at: 513 1065 2460 3849 513
Size: 552 1395 1389 3265
Fragments arranged by size:
3265 1395 1389 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NgoMIV G'CCGG_C
Cuts at: 3119 3119
Size: 6601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NsiI A_TGCA'T
Cuts at: 2652 2652
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 2650 3959 5504 1132
Size: 97 1421 1309 1545 2229
Fragments arranged by size:
2229 1545 1421 1309 97
PasI CC'CwG_GG
Cuts at: 1348 3691 1348
Size: 2343 4258
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 2314 3228 1108
Size: 1194 12 914 4481
Fragments arranged by size:
4481 1194 914 12
PfoI T'CCnGG_A
Cuts at: 3753 3753
Size: 6601
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 4171 4171
Size: 6601
PstI C_TGCA'G
Cuts at: 830 2082 2268 2537 2868 2880 2898 3099 3953
Size: 1252 186 269 331 12 18 201 854
Cuts at: 3953 830
Size: 3478
Fragments arranged by size:
3478 1252 854 331 269 201 186 18
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 2248 2557 2875 3482 3613 2248
Size: 309 318 607 131 5236
Fragments arranged by size:
5236 607 318 309 131
SacI G_AGCT'C
Cuts at: 2946 2946
Size: 6601
SacII CC_GC'GG
Cuts at: 3015 3107 3015
Size: 92 6509
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3099 3953 3099
Size: 854 5747
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SexAI A'CCwGG_T
Cuts at: 2922 2922
Size: 6601
SfcI C'TryA_G
Cuts at: 826 1045 2078 2264 2533 2864 2876 2894 3095
Size: 219 1033 186 269 331 12 18 201
Cuts at: 3095 3949 5084 5765 5956 826
Size: 854 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 854 681 331 269 219
201 191 186 18 12
SfoI GGC'GCC
Cuts at: 2985 2985
Size: 6601
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 3678 1700
Size: 1978 4623
SmaI CCC'GGG
Cuts at: 1872 2633 3928 1872
Size: 761 1295 4545
Fragments arranged by size:
4545 1295 761
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4705 5606 5868 820
Size: 197 926 2762 901 262 1553
Fragments arranged by size:
2762 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3898 4392 493
Size: 3405 494 2702
Fragments arranged by size:
3405 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 2041 2210 1517
Size: 524 169 5908
Fragments arranged by size:
5908 524 169
StyI C'CwwG_G
Cuts at: 513 1065 2412 2460 2988 3727 3849 4040 513
Size: 552 1347 48 528 739 122 191 3074
Fragments arranged by size:
3074 1347 739 552 528 191 122 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4628 4645 4798 4983 5428 325
Size: 1255 3048 17 153 185 445 1498
Fragments arranged by size:
3048 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 4847 6559
Size: 274 80 33 51 1443 660 2210 1712
Cuts at: 6559 96
Size: 138
Fragments arranged by size:
2210 1712 1443 660 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6561 6593 6561
Size: 32 6569
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6601
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 2631 3926 1870
Size: 761 1295 4545
Fragments arranged by size:
4545 1295 761
XmnI GAAnn'nnTTC
Cuts at: 4730 4730
Size: 6601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2257 4409 276
Size: 53 83 186 936 723 2152 2468
Fragments arranged by size:
2468 2152 936 723 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BplI Bpu10I BsiWI
BstZ17I EcoNI EcoRV FseI FspAI NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI
SrfI SwaI XcmI