Restriction map for
pFN21ASDA0127
(Circular) (Six-base) MAPSORT of: pFN21ASDA0127.seq Check: 6274 from: 1 to: 5671
pFN21ASDA0127 5671 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2723 3481 278
Size: 53 83 186 936 1187 758 2468
Fragments arranged by size:
2468 1187 936 758 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2326 3734 5118 5386 1632
Size: 694 1408 1384 268 1917
Fragments arranged by size:
1917 1408 1384 694 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 2223 2445 4570 5547 1203
Size: 1020 222 2125 977 1327
Fragments arranged by size:
2125 1327 1020 977 222
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 3414 4986 3414
Size: 1572 4099
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2704 2980 3206 3358 5467 1493
Size: 1211 276 226 152 2109 1697
Fragments arranged by size:
2109 1697 1211 276 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2429 2996 1870
Size: 73 486 567 4545
Fragments arranged by size:
4545 567 486 73
BamHI G'GATC_C
Cuts at: 2235 3001 3368 2235
Size: 766 367 4538
Fragments arranged by size:
4538 766 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2633 2992 4447 618
Size: 325 217 627 846 359 1455 1842
Fragments arranged by size:
1842 1455 846 627 359 325 217
BanII G_rGCy'C
Cuts at: 1391 2193 2310 2559 2990 1391
Size: 802 117 249 431 4072
Fragments arranged by size:
4072 802 431 249 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2336 4282 136
Size: 107 122 71 631 605 664 1946 1525
Fragments arranged by size:
1946 1525 664 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2484 2605 4331 1239
Size: 1181 64 121 1726 2579
Fragments arranged by size:
2579 1726 1181 121 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2640 3796 4661 4959 2640
Size: 1156 865 298 3352
Fragments arranged by size:
3352 1156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2968 3462 493
Size: 711 1764 494 2702
Fragments arranged by size:
2702 1764 711 494
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2354 2720 3478 3860
Size: 53 83 186 936 821 366 758 382
Cuts at: 3860 4495 275
Size: 635 1451
Fragments arranged by size:
1451 936 821 758 635 382 366 186
83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 2857 2887 1178
Size: 30 165 30 1110 30 314 30 3962
Fragments arranged by size:
3962 1110 314 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2278 2521 2567 2849 1186
Size: 1092 243 46 282 4008
Fragments arranged by size:
4008 1092 282 243 46
BseYI C'CCAG_C
Cuts at: 2192 2292 2309 4874 5370 2192
Size: 100 17 2565 496 2493
Fragments arranged by size:
2565 2493 496 100 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2788 1163
Size: 573 1052 4046
Fragments arranged by size:
4046 1052 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 3732 3817 4888 2559
Size: 1173 85 1071 3342
Fragments arranged by size:
3342 1173 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2310 2559 2990 3732 3817 4888
Size: 399 403 117 249 431 742 85 1071
Cuts at: 4888 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 431 403 399 249 117
85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 4315 4502 1700
Size: 110 525 1980 187 2869
Fragments arranged by size:
2869 1980 525 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 2007 4475 2007
Size: 2468 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2235 3001 3368 3753 3770 5222 5308 5320
Size: 263 766 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 766 385 367 346 263 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2267 2267
Size: 5671
BtgI C'CryG_G
Cuts at: 513 1065 2475 2589 2724 513
Size: 552 1410 114 135 3460
Fragments arranged by size:
3460 1410 552 135 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2423 3187 3981 4001 2423
Size: 764 794 20 4093
Fragments arranged by size:
4093 794 764 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2586 3118 4007
Size: 54 1425 50 275 18 756 532 889
Cuts at: 4007 5636 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 756 532 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2248 4258 4632 4778 387
Size: 1064 138 659 2010 374 146 1280
Fragments arranged by size:
2010 1280 1064 659 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2326 2420 2484 2605 3734 4331 5118
Size: 393 694 94 64 121 1129 597 787
Cuts at: 5118 5386 1239
Size: 268 1524
Fragments arranged by size:
1524 1129 787 694 597 393 268 121
94 64
EcoO109I rG'GnC_Cy
Cuts at: 2178 2329 2616 2961 2178
Size: 151 287 345 4888
Fragments arranged by size:
4888 345 287 151
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2925 2925
Size: 5671
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2134 4818 1678
Size: 456 2684 2531
Fragments arranged by size:
2684 2531 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2513 2545 2677
Size: 32 135 32 518 32 391 32 132
Cuts at: 2677 2709 4312 4344 4386 4418 1373
Size: 32 1603 32 42 32 2626
Fragments arranged by size:
2626 1603 518 391 135 132 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3849
Fragments arranged by size:
3849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2948 3629 3988 4147 518
Size: 648 898 884 681 359 159 2042
Fragments arranged by size:
2042 898 884 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3087 3764 4912 5157 1606
Size: 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 245
NaeI GCC'GGC
Cuts at: 2337 2337
Size: 5671
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5119
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NgoMIV G'CCGG_C
Cuts at: 2335 2335
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2227 3029 4574 1132
Size: 97 998 802 1545 2229
Fragments arranged by size:
2229 1545 998 802 97
PacI TTA_AT'TAA
Cuts at: 2217 2217
Size: 5671
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 2223 4570 2223
Size: 2347 3324
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2944 1108
Size: 1836 3835
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2616 2961 2616
Size: 345 5326
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PspXI vC'TCGA_Gb
Cuts at: 2429 2429
Size: 5671
PstI C_TGCA'G
Cuts at: 830 3023 830
Size: 2193 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 3019 4154 4835 5026 826
Size: 219 1974 1135 681 191 1471
Fragments arranged by size:
1974 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2336 2336
Size: 5671
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 2655 3775 4676 4938 820
Size: 197 926 486 226 1120 901 262 1553
Fragments arranged by size:
1553 1120 926 901 486 262 226 197
SnaBI TAC'GTA
Cuts at: 493 2968 3462 493
Size: 2475 494 2702
Fragments arranged by size:
2702 2475 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 2405 1517
Size: 888 4783
StyI C'CwwG_G
Cuts at: 513 1065 2109 2538 3110 513
Size: 552 1044 429 572 3074
Fragments arranged by size:
3074 1044 572 552 429
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 3698 3715 3868 4053 4498 325
Size: 1255 1071 1047 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 1047 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 5631 5663 2339
Size: 32 3260 32 2347
Fragments arranged by size:
3260 2347 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 2429 1943
Size: 486 5185
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2721 3479 276
Size: 53 83 186 936 1187 758 2468
Fragments arranged by size:
2468 1187 936 758 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PmlI PspOMI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfoI SrfI SwaI XcmI