Restriction map for
pFN21ASDA0119
(Circular) (Six-base) MAPSORT of: pFN21ASDA0119.seq Check: 8991 from: 1 to: 5698
pFN21ASDA0119 5698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 3019 4474 618
Size: 325 217 627 300 932 1455 1842
Fragments arranged by size:
1842 1455 932 627 325 300 217
BanII G_rGCy'C
Cuts at: 1391 3017 1391
Size: 1626 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 928
Size: 971 360 4367
Fragments arranged by size:
4367 971 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2732 3429 4387
Size: 416 53 83 399 24 1315 697 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1315 958 697 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2961 1056
Size: 1905 3793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2561 4358 1239
Size: 846 476 1797 2579
Fragments arranged by size:
2579 1797 846 476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 3823 4688 4986 2552
Size: 1271 865 298 3264
Fragments arranged by size:
3264 1271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2995 3489 493
Size: 711 1791 494 2702
Fragments arranged by size:
2702 1791 711 494
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2616 4131 4529 4803 4950 5361 2616
Size: 1515 398 274 147 411 2953
Fragments arranged by size:
2953 1515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 1178
Size: 30 165 30 1296 30 4147
Fragments arranged by size:
4147 1296 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4674
BseYI C'CCAG_C
Cuts at: 2153 2776 4901 5397 2153
Size: 623 2125 496 2454
Fragments arranged by size:
2454 2125 623 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2885 1163
Size: 573 1149 3976
Fragments arranged by size:
3976 1149 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2677 2727 3759 3844 4915 2677
Size: 50 1032 85 1071 3460
Fragments arranged by size:
3460 1071 1032 85 50
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2677 2727 3017 3759 3844 4915 1391
Size: 399 887 50 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 887 742 399 290 85 50
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2191 2857 3053 844
Size: 1347 666 196 3489
Fragments arranged by size:
3489 1347 666 196
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 2007 4502 2007
Size: 2495 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2449 2681 3028 3395 3780 3797 5249 5335
Size: 477 232 347 367 385 17 1452 86
Cuts at: 5335 5347 5693 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 477 385 367 347 346 232
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2472 513
Size: 552 957 450 3739
Fragments arranged by size:
3739 957 552 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2045 2245 3214 4008 4028 2045
Size: 200 969 794 20 3715
Fragments arranged by size:
3715 969 794 200 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 2211 2499
Size: 54 1425 50 275 18 229 152 288
Cuts at: 2499 2826 3145 4034 5663 8
Size: 327 319 889 1629 43
Fragments arranged by size:
1629 1425 889 327 319 288 275 229
152 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3628 1353
Size: 887 1388 3423
Fragments arranged by size:
3423 1388 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 4285 4659 4805 387
Size: 1064 138 561 2135 374 146 1280
Fragments arranged by size:
2135 1280 1064 561 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2561 3761 4358 5145 5413 1239
Size: 393 453 476 1200 597 787 268 1524
Fragments arranged by size:
1524 1200 787 597 476 453 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2734 2734
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2139 2139
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2199 2231 2699 2731 4339
Size: 32 135 32 627 32 468 32 1608
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1608 627 468 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2356 2886 3042 3288 669
Size: 988 281 418 530 156 246 3079
Fragments arranged by size:
3079 988 530 418 281 246 156
HindIII A'AGCT_T
Cuts at: 748 2121 2273 748
Size: 1373 152 4173
Fragments arranged by size:
4173 1373 152
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 2342 3297 2342
Size: 955 4743
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 2332 4812 4996 1305
Size: 880 147 2480 184 2007
Fragments arranged by size:
2480 2007 880 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2061 2213 2501 2828 10
Size: 54 1425 343 229 152 288 327 2880
Fragments arranged by size:
2880 1425 343 327 288 229 152 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3656 4015 4174 518
Size: 648 1347 1143 359 159 2042
Fragments arranged by size:
2042 1347 1143 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2539 2803 3114 3791 4939 5184 1606
Size: 933 264 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 677 311 264 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2472 513
Size: 552 957 450 3739
Fragments arranged by size:
3739 957 552 450
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 2957 1052
Size: 1905 3793
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2734 2734
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2808 2893 3050 830
Size: 1978 85 157 3478
Fragments arranged by size:
3478 1978 157 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2539 2803 2539
Size: 264 5434
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SexAI A'CCwGG_T
Cuts at: 2589 2589
Size: 5698
SfcI C'TryA_G
Cuts at: 826 1045 2668 2804 2889 3046 4181 4862 5053
Size: 219 1623 136 85 157 1135 681 191
Cuts at: 5053 826
Size: 1471
Fragments arranged by size:
1623 1471 1135 681 219 191 157 136
85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 3802 4703 4965 820
Size: 197 926 624 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 624 262 197
SnaBI TAC'GTA
Cuts at: 493 2995 3489 493
Size: 2502 494 2702
Fragments arranged by size:
2702 2502 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 2436 2990 3622 5
Size: 47 2384 554 632 2081
Fragments arranged by size:
2384 2081 632 554 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2022 2316 2472 2751 3137 513
Size: 552 957 294 156 279 386 3074
Fragments arranged by size:
3074 957 552 386 294 279 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2692 3725 3742 3895 4080 4525 325
Size: 1255 1112 1033 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1112 1033 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 3944 5656
Size: 274 80 33 51 1443 594 1373 1712
Cuts at: 5656 96
Size: 138
Fragments arranged by size:
1712 1443 1373 594 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 2738 1316
Size: 1422 4276
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2807 2807
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI