Restriction map for
pFN21ASDA0115
(Circular) (Six-base) MAPSORT of: pFN21ASDA0115.seq Check: 2354 from: 1 to: 6097
pFN21ASDA0115 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2033
Size: 6097
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2585 2776 2797 3146 3331 4160 5544
Size: 611 342 191 21 349 185 829 1384
Cuts at: 5544 5812 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 829 611 349 342 268 191
185 21
AfeI AGC'GCT
Cuts at: 3281 3281
Size: 6097
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 3171 4996 5973 1203
Size: 1968 1825 977 1327
Fragments arranged by size:
1968 1825 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 2165 1166
Size: 999 5098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2183 1184
Size: 999 5098
AlwNI CAG_nnn'CTG
Cuts at: 2048 3840 5412 2048
Size: 1792 1572 2733
Fragments arranged by size:
2733 1792 1572
ApaI G_GGCC'C
Cuts at: 2107 2107
Size: 6097
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2294 3406 3632 3784 5893 1493
Size: 801 1112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 801 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3422 1870
Size: 73 151 1328 4545
Fragments arranged by size:
4545 1328 151 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3228 3261 3228
Size: 33 6064
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 3418 4873 618
Size: 325 217 627 1064 567 1455 1842
Fragments arranged by size:
1842 1455 1064 627 567 325 217
BanII G_rGCy'C
Cuts at: 1391 2082 2107 2244 2675 2763 3339 3416 1391
Size: 691 25 137 431 88 576 77 4072
Fragments arranged by size:
4072 691 576 431 137 88 77 25
BbeI G_GCGC'C
Cuts at: 2855 2855
Size: 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3349 928
Size: 971 1450 3676
Fragments arranged by size:
3676 1450 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2730 3256 3989 5199 2730
Size: 526 733 1210 3628
Fragments arranged by size:
3628 1210 733 526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2107 4158 5314 1790
Size: 317 2051 1156 2573
Fragments arranged by size:
2573 2051 1156 317
BmgBI CAC'GTC
Cuts at: 1318 3172 1318
Size: 1854 4243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3828 4786
Size: 416 53 83 399 24 1293 1118 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1293 1118 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2747 3107 3245 4757 1239
Size: 1508 360 138 1512 2579
Fragments arranged by size:
2579 1512 1508 360 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 4760 882
Size: 617 858 2403 2219
Fragments arranged by size:
2403 2219 858 617
BsaAI yAC'GTr
Cuts at: 493 1204 3394 3888 493
Size: 711 2190 494 2702
Fragments arranged by size:
2702 2190 711 494
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2852 3904 4286 4921
Size: 53 83 186 936 1319 1052 382 635
Cuts at: 4921 275
Size: 1451
Fragments arranged by size:
1451 1319 1052 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2034 2214 4530 4928 5202 5349 5760 2034
Size: 180 2316 398 274 147 411 2371
Fragments arranged by size:
2371 2316 411 398 274 180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2382 2412 1178
Size: 30 165 30 979 30 4863
Fragments arranged by size:
4863 979 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2489 2738 2942 1186
Size: 894 409 249 204 4341
Fragments arranged by size:
4341 894 409 249 204
BseYI C'CCAG_C
Cuts at: 2048 2081 2267 2443 2756 2913 3331 5300 5796
Size: 33 186 176 313 157 418 1969 496
Cuts at: 5796 2048
Size: 2349
Fragments arranged by size:
2349 1969 496 418 313 186 176 157
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2061 2861 1163
Size: 573 325 800 4399
Fragments arranged by size:
4399 800 573 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 4158 4243 5314 2244
Size: 1914 85 1071 3027
Fragments arranged by size:
3027 1914 1071 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2107 2244 2675 2763 3339 3416
Size: 399 292 25 137 431 88 576 77
Cuts at: 3416 4158 4243 5314 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 576 431 399 292 137
88 85 77 25
BspEI T'CCGG_A
Cuts at: 2214 5760 2214
Size: 3546 2551
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 2568 3452 844
Size: 1189 535 884 3489
Fragments arranged by size:
3489 1189 884 535
BsrBI CCG'CTC
Cuts at: 1394 1823 3984 1394
Size: 429 2161 3507
Fragments arranged by size:
3507 2161 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2751 4592 4766 65
Size: 1183 1503 1841 174 1396
Fragments arranged by size:
1841 1503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3207 4741 4928 1700
Size: 110 1397 1534 187 2869
Fragments arranged by size:
2869 1534 1397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2454 794
Size: 1660 4437
BstBI TT'CG_AA
Cuts at: 2007 4901 2007
Size: 2894 3203
BstEII G'GTnAC_C
Cuts at: 1184 2426 1184
Size: 1242 4855
BstXI CCAn_nnnn'nTGG
Cuts at: 3331 3331
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092
Size: 1455 367 385 17 1452 86 12 346
Cuts at: 6092 1972
Size: 1977
Fragments arranged by size:
1977 1455 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2088 513
Size: 552 957 66 4522
Fragments arranged by size:
4522 957 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3087 487
Size: 683 56 1861 3497
Fragments arranged by size:
3497 1861 683 56
BtsI GCAGTG_nn'
Cuts at: 2401 2455 2844 3613 4407 4427 2401
Size: 54 389 769 794 20 4071
Fragments arranged by size:
4071 794 769 389 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3193 3401 3193
Size: 208 5889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2524 2631 3254
Size: 54 1425 50 275 18 694 107 623
Cuts at: 3254 3544 4433 6062 8
Size: 290 889 1629 43
Fragments arranged by size:
1629 1425 889 694 623 290 275 107
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 2680 3238 3344 4027 1353
Size: 1086 241 558 106 683 3423
Fragments arranged by size:
3423 1086 683 558 241 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2403 4684 5058 5204 387
Size: 1064 138 814 2281 374 146 1280
Fragments arranged by size:
2281 1280 1064 814 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2242
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2585 2747 2776 2797 3107 3146
Size: 393 611 342 162 29 21 310 39
Cuts at: 3146 3245 3331 4160 4757 5544 5812 1239
Size: 99 86 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 611 597 393 342 310
268 162 99 86 39 29 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2059 2564 2059
Size: 505 5592
EcoO109I rG'GnC_Cy
Cuts at: 2052 2104 2569 2948 3058 3117 2052
Size: 52 465 379 110 59 5032
Fragments arranged by size:
5032 465 379 110 59 52
EcoRI G'AATT_C
Cuts at: 2294 3406 3784 2294
Size: 1112 378 4607
Fragments arranged by size:
4607 1112 378
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2855 3283 3318 5244 1678
Size: 1177 428 35 1926 2531
Fragments arranged by size:
2531 1926 1177 428 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 4738 4770 4812
Size: 32 135 32 765 32 2369 32 42
Cuts at: 4812 4844 1373
Size: 32 2626
Fragments arranged by size:
2626 2369 765 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2851 2851
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 3411 5211 5395 1305
Size: 1137 969 1800 184 2007
Fragments arranged by size:
2007 1800 1137 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2526 10
Size: 54 1425 343 694 3581
Fragments arranged by size:
3581 1425 694 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2357 2505 4055 4414 4573 518
Size: 648 999 192 148 1550 359 159 2042
Fragments arranged by size:
2042 1550 999 648 359 192 159 148
MspA1I CmG'CkG
Cuts at: 1606 2090 2151 2258 2796 2913 3085 3513 4190
Size: 484 61 107 538 117 172 428 677
Cuts at: 4190 5338 5583 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 538 484 428 245 172
117 107 61
NarI GG'CG_CC
Cuts at: 2852 2852
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4588
Fragments arranged by size:
4588 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2729 3455 5000 1132
Size: 97 1500 726 1545 2229
Fragments arranged by size:
2229 1545 1500 726 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PfoI T'CCnGG_A
Cuts at: 2697 2697
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2052 3058 3117 2052
Size: 1006 59 5032
Fragments arranged by size:
5032 1006 59
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2103 2103
Size: 6097
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 3085 3085
Size: 6097
SacI G_AGCT'C
Cuts at: 2244 2244
Size: 6097
SacII CC_GC'GG
Cuts at: 2091 2091
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SanDI GG'GwC_CC
Cuts at: 3058 3058
Size: 6097
SapI GCTCTTCn'nnn_
Cuts at: 2680 3344 2680
Size: 664 5433
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 3445 4580 5261 5452 826
Size: 219 2400 1135 681 191 1471
Fragments arranged by size:
2400 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2853 2853
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 2096 3424 1872
Size: 224 1328 4545
Fragments arranged by size:
4545 1328 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3394 3888 493
Size: 2901 494 2702
Fragments arranged by size:
2901 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SrfI GCCC'GGGC
Cuts at: 2096 2096
Size: 6097
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2022 2521 3536 513
Size: 552 957 499 1015 3074
Fragments arranged by size:
3074 1015 957 552 499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2729 2905 3001 4124 4141 4294 4479
Size: 1255 1149 176 96 1123 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1149 1123 445 185 176 153
96 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3228 4343 6055
Size: 274 80 33 51 1443 1251 1115 1712
Cuts at: 6055 96
Size: 138
Fragments arranged by size:
1712 1443 1251 1115 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 2704 2736 6057 6089 2382
Size: 32 290 32 3321 32 2390
Fragments arranged by size:
3321 2390 290 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 2094 3422 1870
Size: 224 1328 4545
Fragments arranged by size:
4545 1328 224
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SexAI SfiI SwaI XcmI