Restriction map for
pFN21ASDA0112
(Circular) (Six-base) MAPSORT of: pFN21ASDA0112.seq Check: 8228 from: 1 to: 5824
pFN21ASDA0112 5824 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3887 5271 5539 1632
Size: 2255 1384 268 1917
Fragments arranged by size:
2255 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2114 1184
Size: 930 4894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 2516
Size: 32 5792
AlwNI CAG_nnn'CTG
Cuts at: 2212 3567 5139 2212
Size: 1355 1572 2897
Fragments arranged by size:
2897 1572 1355
ApaI G_GGCC'C
Cuts at: 3002 3002
Size: 5824
ApaLI G'TGCA_C
Cuts at: 2099 3881 5037 2099
Size: 1782 1156 2886
Fragments arranged by size:
2886 1782 1156
ApoI r'AATT_y
Cuts at: 1493 3049 3133 3359 3511 5620 1493
Size: 1556 84 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2469 2757 3149 1870
Size: 73 526 288 392 4545
Fragments arranged by size:
4545 526 392 288 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2500 2752 3145 4600 618
Size: 325 217 627 713 252 393 1455 1842
Fragments arranged by size:
1842 1455 713 627 393 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2307 2765 3002 3143 1391
Size: 916 458 237 141 4072
Fragments arranged by size:
4072 916 458 237 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2411 928
Size: 971 512 4341
Fragments arranged by size:
4341 971 512
BbvCI CC'TCA_GC
Cuts at: 2862 2862
Size: 5824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2152 2186 4015 4049 1903
Size: 34 215 34 1829 34 3678
Fragments arranged by size:
3678 1829 215 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2385 2949 4435
Size: 107 122 71 631 605 713 564 1486
Cuts at: 4435 136
Size: 1525
Fragments arranged by size:
1525 1486 713 631 605 564 122 107
71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2505 2757 3002 3885 5041 1790
Size: 313 402 252 245 883 1156 2573
Fragments arranged by size:
2573 1156 883 402 313 252 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 4513 442
Size: 416 53 83 399 24 2138 958 1753
Fragments arranged by size:
2138 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 4484 1239
Size: 893 2352 2579
Fragments arranged by size:
2579 2352 893
Bpu10I CC'TnA_GC
Cuts at: 2862 2862
Size: 5824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 3949 4814 5112 2366
Size: 1583 865 298 3078
Fragments arranged by size:
3078 1583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2707 4487 882
Size: 617 1208 1780 2219
Fragments arranged by size:
2219 1780 1208 617
BsaAI yAC'GTr
Cuts at: 493 1204 3121 3615 493
Size: 711 1917 494 2702
Fragments arranged by size:
2702 1917 711 494
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2144 3631 4013 4648
Size: 53 83 186 936 611 1487 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1487 1451 936 635 611 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2151 2651 4257 4655 4929 5076 5487 2151
Size: 500 1606 398 274 147 411 2488
Fragments arranged by size:
2488 1606 500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 2516 2546 1178
Size: 30 165 30 702 30 381 30 4456
Fragments arranged by size:
4456 702 381 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2684 2918 2930 2944 3124 1186
Size: 871 627 234 12 14 180 3886
Fragments arranged by size:
3886 871 627 234 180 14 12
BseYI C'CCAG_C
Cuts at: 2451 2627 2674 3005 5027 5523 2451
Size: 176 47 331 2022 496 2752
Fragments arranged by size:
2752 2022 496 331 176 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2166 2345 2637 3274 4035 5063
Size: 275 186 165 179 292 637 761 1028
Cuts at: 5063 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 637 292 275 186 179
165
BsiHKAI G_wGCw'C
Cuts at: 2103 3885 3970 5041 2103
Size: 1782 85 1071 2886
Fragments arranged by size:
2886 1782 1071 85
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2307 2505 2757 2765 3002 3143
Size: 399 313 204 198 252 8 237 141
Cuts at: 3143 3885 3970 5041 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 313 252 237 204
198 141 85 8
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 2844 3713 5443 2844
Size: 869 1730 3225
Fragments arranged by size:
3225 1730 869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 3179 844
Size: 1301 1034 3489
Fragments arranged by size:
3489 1301 1034
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2515 4319 4493 65
Size: 1183 1267 1804 174 1396
Fragments arranged by size:
1804 1396 1267 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2586 2636 2967 4468 4655 1700
Size: 110 776 50 331 1501 187 2869
Fragments arranged by size:
2869 1501 776 331 187 110 50
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 2375 2464 2607 1925
Size: 450 89 143 5142
Fragments arranged by size:
5142 450 143 89
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2617 794
Size: 1823 4001
BstBI TT'CG_AA
Cuts at: 2007 4628 2007
Size: 2621 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2692 2692
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2262 2345 513
Size: 552 957 240 83 3992
Fragments arranged by size:
3992 957 552 240 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 4134 4154 3340
Size: 794 20 5010
Fragments arranged by size:
5010 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2348 2434 2568
Size: 54 1425 50 275 18 518 86 134
Cuts at: 2568 2634 2694 3271 4160 5789 8
Size: 66 60 577 889 1629 43
Fragments arranged by size:
1629 1425 889 577 518 275 134 86
66 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2634 3271 1537
Size: 275 822 637 4090
Fragments arranged by size:
4090 822 637 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 3014 3754 1353
Size: 918 743 740 3423
Fragments arranged by size:
3423 918 743 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 3887 4484 5271 5539 1239
Size: 393 500 1755 597 787 268 1524
Fragments arranged by size:
1755 1524 787 597 500 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2741 2741
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 2209 2975 2998 2209
Size: 766 23 5035
Fragments arranged by size:
5035 766 23
EcoRI G'AATT_C
Cuts at: 3049 3133 3511 3049
Size: 84 378 5362
Fragments arranged by size:
5362 378 84
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2452 2725 2737 3097 4971 1678
Size: 774 273 12 360 1874 2531
Fragments arranged by size:
2531 1874 774 360 273 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373
Size: 32 135 32 2893 32 42 32 2626
Fragments arranged by size:
2893 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2808 4938 5122 1305
Size: 822 681 2130 184 2007
Fragments arranged by size:
2130 2007 822 681 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2436 2570 2696 10
Size: 54 1425 343 604 134 126 3138
Fragments arranged by size:
3138 1425 604 343 134 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2690 3782 4141 4300 518
Size: 648 930 594 1092 359 159 2042
Fragments arranged by size:
2042 1092 930 648 594 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2257 2347 2623 2866 3240 3917 5065
Size: 642 9 90 276 243 374 677 1148
Cuts at: 5065 5310 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 642 374 276 245 243
90 9
NaeI GCC'GGC
Cuts at: 2638 2969 2638
Size: 331 5493
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4315
Fragments arranged by size:
4315 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2636 2967 2636
Size: 331 5493
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2038 2487 1108
Size: 930 449 4445
Fragments arranged by size:
4445 930 449
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 2548 3893 3925 5406 5438 2516
Size: 32 1345 32 1481 32 2902
Fragments arranged by size:
2902 1481 1345 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2998 2998
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2121 3176 830
Size: 1291 1055 3478
Fragments arranged by size:
3478 1291 1055
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2248 2257 2623 2866 2248
Size: 9 366 243 5206
Fragments arranged by size:
5206 366 243 9
RsrII CG'GwC_CG
Cuts at: 2149 2149
Size: 5824
SacII CC_GC'GG
Cuts at: 2348 2348
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SexAI A'CCwGG_T
Cuts at: 2412 2412
Size: 5824
SfcI C'TryA_G
Cuts at: 826 1045 2117 2204 3172 4307 4988 5179 826
Size: 219 1072 87 968 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 968 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2471 2759 3151 1872
Size: 599 288 392 4545
Fragments arranged by size:
4545 599 392 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2381 3928 4829 5091 820
Size: 197 926 438 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 438 262 197
SnaBI TAC'GTA
Cuts at: 493 3121 3615 493
Size: 2628 494 2702
Fragments arranged by size:
2702 2628 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2022 2520 2697 2742 3263 513
Size: 552 957 498 177 45 521 3074
Fragments arranged by size:
3074 957 552 521 498 177 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3059 3851 3868 4021 4206 4651 325
Size: 1255 1479 792 17 153 185 445 1498
Fragments arranged by size:
1498 1479 1255 792 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2358 2713 2269
Size: 89 355 5380
Fragments arranged by size:
5380 355 89
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2469 2757 3149 1870
Size: 599 288 392 4545
Fragments arranged by size:
4545 599 392 288
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI SacI SanDI SapI SfiI
SfoI SrfI SwaI