Restriction map for
pFN21ASDA0111
(Circular) (Six-base) MAPSORT of: pFN21ASDA0111.seq Check: 5342 from: 1 to: 5962
pFN21ASDA0111 5962 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2634 3305 1937
Size: 697 671 4594
Fragments arranged by size:
4594 697 671
Acc65I G'GTAC_C
Cuts at: 2640 3283 2640
Size: 643 5319
AclI AA'CG_TT
Cuts at: 1563 3240 4089 4462 1563
Size: 1677 849 373 3063
Fragments arranged by size:
3063 1677 849 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2281 4025 5409 5677 1632
Size: 649 1744 1384 268 1917
Fragments arranged by size:
1917 1744 1384 649 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2555 4861 5838 1203
Size: 1352 2306 977 1327
Fragments arranged by size:
2306 1352 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 2660 1166
Size: 1494 4468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2102 2813 3271 3497 3649 5758 1493
Size: 609 711 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 711 609 458 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 3287 1870
Size: 73 107 1237 4545
Fragments arranged by size:
4545 1237 107 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 2640 2999 3283 4738
Size: 325 217 627 663 190 359 284 1455
Cuts at: 4738 618
Size: 1842
Fragments arranged by size:
1842 1455 663 627 359 325 284 217
190
BanII G_rGCy'C
Cuts at: 1391 2057 3281 1391
Size: 666 1224 4072
Fragments arranged by size:
4072 1224 666
BbeI G_GCGC'C
Cuts at: 3003 3003
Size: 5962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 928
Size: 971 192 4799
Fragments arranged by size:
4799 971 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2944 3854 5064 2944
Size: 910 1210 3842
Fragments arranged by size:
3842 1210 910
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BfrBI ATG'CAT
Cuts at: 2944 2944
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 4573 136
Size: 107 122 71 631 605 370 2531 1525
Fragments arranged by size:
2531 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2510 4023 5179 1790
Size: 720 1513 1156 2573
Fragments arranged by size:
2573 1513 1156 720
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2496 2634 2720 4622 1239
Size: 1058 199 138 86 1902 2579
Fragments arranged by size:
2579 1902 1058 199 138 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2328 4087 4952 5250 2328
Size: 1759 865 298 3040
Fragments arranged by size:
3040 1759 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 4625 882
Size: 617 603 2523 2219
Fragments arranged by size:
2523 2219 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 2558 3259 3753 493
Size: 711 1354 701 494 2702
Fragments arranged by size:
2702 1354 711 701 494
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3000 3769 4151 4786
Size: 53 83 186 936 1467 769 382 635
Cuts at: 4786 275
Size: 1451
Fragments arranged by size:
1467 1451 936 769 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2991 4395 4793 5067 5214 5625 2991
Size: 1404 398 274 147 411 3328
Fragments arranged by size:
3328 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2978 3008 3112 3142 1178
Size: 30 165 30 1575 30 104 30 3998
Fragments arranged by size:
3998 1575 165 104 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 1186
Size: 946 5016
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 1163
Size: 573 666 4723
Fragments arranged by size:
4723 666 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2996 3139 3412 4173 5201 1540
Size: 275 186 995 143 273 761 1028 2301
Fragments arranged by size:
2301 1028 995 761 275 273 186 143
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2510 3281 4023 4108 5179 1391
Size: 399 267 453 771 742 85 1071 2174
Fragments arranged by size:
2174 1071 771 742 453 399 267 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2718 2979 3851 5581 2718
Size: 261 872 1730 3099
Fragments arranged by size:
3099 1730 872 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2652 3317 844
Size: 1331 477 665 3489
Fragments arranged by size:
3489 1331 665 477
BsrBI CCG'CTC
Cuts at: 1394 1823 2968 3849 1394
Size: 429 1145 881 3507
Fragments arranged by size:
3507 1145 881 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 3102 96
Size: 3006 2956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 2690 4022 5034 2690
Size: 1332 1012 3618
Fragments arranged by size:
3618 1332 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 2007 4766 2007
Size: 2759 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 2662 3043 2662
Size: 381 5581
BstYI r'GATC_y
Cuts at: 1972 2385 3292 3659 4044 4061 5513 5599 5611
Size: 413 907 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 413 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2635 2635
Size: 5962
Bsu36I CC'TnA_GG
Cuts at: 2549 3054 2549
Size: 505 5457
BtgI C'CryG_G
Cuts at: 513 1065 2022 2031 2148 513
Size: 552 957 9 117 4327
Fragments arranged by size:
4327 957 552 117 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2242 487
Size: 683 56 1016 4207
Fragments arranged by size:
4207 1016 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3478 4272 4292 2799
Size: 679 794 20 4469
Fragments arranged by size:
4469 794 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3202 3237 529
Size: 35 2638 35 3254
Fragments arranged by size:
3254 2638 35 35
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DraIII CAC_nnn'GTG
Cuts at: 2826 2826
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3136 3150
Size: 54 1425 50 275 18 204 1102 14
Cuts at: 3150 3409 4298 5927 8
Size: 259 889 1629 43
Fragments arranged by size:
1629 1425 1102 889 275 259 204 54
50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 3136 3409 1537
Size: 275 1324 273 4090
Fragments arranged by size:
4090 1324 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 3892 1353
Size: 1446 1093 3423
Fragments arranged by size:
3423 1446 1093
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2281 2297 2496 2634 2720 4025 4622
Size: 393 649 16 199 138 86 1305 597
Cuts at: 4622 5409 5677 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1305 787 649 597 393 268 199
138 86 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2159
Size: 5962
EcoO109I rG'GnC_Cy
Cuts at: 2164 2164
Size: 5962
EcoRI G'AATT_C
Cuts at: 2102 3271 3649 2102
Size: 1169 378 4415
Fragments arranged by size:
4415 1169 378
EcoRV GAT'ATC
Cuts at: 3196 3196
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 3003 5109 1678
Size: 1325 2106 2531
Fragments arranged by size:
2531 2106 1325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 2344 2376 2839
Size: 32 135 32 669 32 71 32 463
Cuts at: 2839 2871 2957 2978 2989 3010 3112 3144 4603
Size: 32 86 21 11 21 102 32 1459
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1459 669 463 135 102 86 71
42 32 32 32 32 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2346 748
Size: 1598 4364
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KasI G'GCGC_C
Cuts at: 2999 2999
Size: 5962
KpnI G_GTAC'C
Cuts at: 2644 3287 2644
Size: 643 5319
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 5076 5260 1305
Size: 1719 2052 184 2007
Fragments arranged by size:
2052 2007 1719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3152 10
Size: 54 1425 343 1320 2820
Fragments arranged by size:
2820 1425 1320 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 2741 3920 4279 4438 518
Size: 648 1494 81 1179 359 159 2042
Fragments arranged by size:
2042 1494 1179 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 3378 4055 5203 5448 1606
Size: 1772 677 1148 245 2120
Fragments arranged by size:
2120 1772 1148 677 245
NarI GG'CG_CC
Cuts at: 3000 3000
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 2022 2148 513
Size: 552 957 126 4327
Fragments arranged by size:
4327 957 552 126
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NsiI A_TGCA'T
Cuts at: 2946 2946
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2498 2956 3320 4865 1132
Size: 97 1269 458 364 1545 2229
Fragments arranged by size:
2229 1545 1269 458 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 4750
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PmlI CAC'GTG
Cuts at: 2558 2558
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 3206 4210 5922 3206
Size: 1004 1712 3246
Fragments arranged by size:
3246 1712 1004
SfcI C'TryA_G
Cuts at: 826 1045 3021 3310 4445 5126 5317 826
Size: 219 1976 289 1135 681 191 1471
Fragments arranged by size:
1976 1471 1135 681 289 219 191
SfoI GGC'GCC
Cuts at: 3001 3001
Size: 5962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2343 4066 4967 5229 820
Size: 197 926 400 1723 901 262 1553
Fragments arranged by size:
1723 1553 926 901 400 262 197
SnaBI TAC'GTA
Cuts at: 493 3259 3753 493
Size: 2766 494 2702
Fragments arranged by size:
2766 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2022 2148 3401 513
Size: 552 957 126 1253 3074
Fragments arranged by size:
3074 1253 957 552 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2738 3989 4006 4159 4344 4789 325
Size: 1255 1158 1251 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1251 1158 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3102 3204 4208
Size: 274 80 33 51 1443 1125 102 1004
Cuts at: 4208 5920 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1125 1004 274 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2119 2371 2441 2887 2038
Size: 81 252 70 446 5113
Fragments arranged by size:
5113 446 252 81 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 2987 4091 2987
Size: 1104 4858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI