Restriction map for
pFN21ASDA0106
(Circular) (Six-base) MAPSORT of: pFN21ASDA0106.seq Check: 8646 from: 1 to: 5401
pFN21ASDA0106 5401 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2625 3464 4848 5116 1632
Size: 839 154 839 1384 268 1917
Fragments arranged by size:
1917 1384 839 839 268 154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5401
AlwNI CAG_nnn'CTG
Cuts at: 3144 4716 3144
Size: 1572 3829
ApaLI G'TGCA_C
Cuts at: 2161 3458 4614 2161
Size: 1297 1156 2948
Fragments arranged by size:
2948 1297 1156
ApoI r'AATT_y
Cuts at: 1493 2201 2710 2936 3088 5197 1493
Size: 708 509 226 152 2109 1697
Fragments arranged by size:
2109 1697 708 509 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2142 2726 1870
Size: 73 99 100 584 4545
Fragments arranged by size:
4545 584 100 99 73
BamHI G'GATC_C
Cuts at: 2374 2731 3098 2374
Size: 357 367 4677
Fragments arranged by size:
4677 367 357
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 2722 4177 618
Size: 325 217 627 407 528 1455 1842
Fragments arranged by size:
1842 1455 627 528 407 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 2655 2720 1391
Size: 913 351 65 4072
Fragments arranged by size:
4072 913 351 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2632 928
Size: 971 733 3697
Fragments arranged by size:
3697 971 733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3592 3626 1903
Size: 34 1655 34 3678
Fragments arranged by size:
3678 1655 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2818 2818
Size: 5401
Bme1580I G_kGCm'C
Cuts at: 1790 2165 3462 4618 1790
Size: 375 1297 1156 2573
Fragments arranged by size:
2573 1297 1156 375
BmgBI CAC'GTC
Cuts at: 1318 2050 1318
Size: 732 4669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 2549 3132
Size: 416 53 83 399 24 733 399 583
Cuts at: 3132 4090 442
Size: 958 1753
Fragments arranged by size:
1753 958 733 583 416 399 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2287 2507 4061 1239
Size: 1048 220 1554 2579
Fragments arranged by size:
2579 1554 1048 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3526 4391 4689 3526
Size: 865 298 4238
Fragments arranged by size:
4238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4064 882
Size: 617 2565 2219
Fragments arranged by size:
2565 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2698 3192 493
Size: 711 1494 494 2702
Fragments arranged by size:
2702 1494 711 494
BsaBI GATnn'nnATC
Cuts at: 2340 5395 2340
Size: 3055 2346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3208 3590 4225 275
Size: 53 83 186 936 1675 382 635 1451
Fragments arranged by size:
1675 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5176
Fragments arranged by size:
5176 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5401
BseYI C'CCAG_C
Cuts at: 2470 2688 4604 5100 2470
Size: 218 1916 496 2771
Fragments arranged by size:
2771 1916 496 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4828
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2086 2851 3612 4640 1540
Size: 275 186 85 765 761 1028 2301
Fragments arranged by size:
2301 1028 765 761 275 186 85
BsiHKAI G_wGCw'C
Cuts at: 2165 2655 3462 3547 4618 2165
Size: 490 807 85 1071 2948
Fragments arranged by size:
2948 1071 807 490 85
BsmI GAATG_Cn'
Cuts at: 2124 2912 3005 2124
Size: 788 93 4520
Fragments arranged by size:
4520 788 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2304 2655 2720 3462 3547 4618
Size: 399 375 139 351 65 742 85 1071
Cuts at: 4618 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 375 351 139 85
65
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 844
Size: 1912 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4045 4232 1700
Size: 110 2235 187 2869
Fragments arranged by size:
2869 2235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 2415 3461 4473 2415
Size: 1046 1012 3343
Fragments arranged by size:
3343 1046 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5401
BstBI TT'CG_AA
Cuts at: 2007 4205 2007
Size: 2198 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2562 2362
Size: 200 5201
BstYI r'GATC_y
Cuts at: 1972 2374 2731 3098 3483 3500 4952 5038 5050
Size: 402 357 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 402 385 367 357 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4849
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 2331 1554
Size: 777 4624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2155 2190 2539 2574 529
Size: 35 1591 35 349 35 3356
Fragments arranged by size:
3356 1591 349 35 35 35
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2848 3737
Size: 54 1425 50 275 18 253 765 889
Cuts at: 3737 5366 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 765 275 253 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2083 2848 1537
Size: 275 271 765 4090
Fragments arranged by size:
4090 765 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 2292 3331 1353
Size: 782 157 1039 3423
Fragments arranged by size:
3423 1039 782 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 2653
Size: 5401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2471 2507 2625 3464 4061 4848
Size: 393 655 184 36 118 839 597 787
Cuts at: 4848 5116 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 655 597 393 268 184
118 36
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
EcoRV GAT'ATC
Cuts at: 2278 2278
Size: 5401
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 4548 1678
Size: 2870 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 4042 4074 4116
Size: 32 135 32 1070 32 1368 32 42
Cuts at: 4116 4148 1373
Size: 32 2626
Fragments arranged by size:
2626 1368 1070 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 2290 3000 2290
Size: 710 4691
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2618 4515 4699 1305
Size: 1313 1897 184 2007
Fragments arranged by size:
2007 1897 1313 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3579
Fragments arranged by size:
3579 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 3359 3718 3877 518
Size: 648 1239 954 359 159 2042
Fragments arranged by size:
2042 1239 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2817 3494 4642 4887 1606
Size: 921 290 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 290 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4849
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2371 2404 2759 4304 1132
Size: 97 1142 33 355 1545 2229
Fragments arranged by size:
2229 1545 1142 355 97 33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2753 830
Size: 1923 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
PvuII CAG'CTG
Cuts at: 2527 2527
Size: 5401
SacI G_AGCT'C
Cuts at: 2655 2655
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SapI GCTCTTCn'nnn_
Cuts at: 2292 2292
Size: 5401
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SfcI C'TryA_G
Cuts at: 826 1045 2749 3884 4565 4756 826
Size: 219 1704 1135 681 191 1471
Fragments arranged by size:
1704 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3505 4406 4668 820
Size: 197 926 1562 901 262 1553
Fragments arranged by size:
1562 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2698 3192 493
Size: 2205 494 2702
Fragments arranged by size:
2702 2205 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2371 2404 2759 1132
Size: 1239 33 355 3774
Fragments arranged by size:
3774 1239 355 33
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2840 513
Size: 552 1775 3074
Fragments arranged by size:
3074 1775 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3428 3445 3598 3783 4228 325
Size: 1255 1848 17 153 185 445 1498
Fragments arranged by size:
1848 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3647 5359 96
Size: 274 80 33 51 1443 1670 1712 138
Fragments arranged by size:
1712 1670 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI