Restriction map for
pFN21ASDA0096
(Circular) (Six-base) MAPSORT of: pFN21ASDA0096.seq Check: 5073 from: 1 to: 7024
pFN21ASDA0096 7024 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2611 2611
Size: 7024
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4834 278
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4367 1937
Size: 2430 4594
Acc65I G'GTAC_C
Cuts at: 3884 4345 3884
Size: 461 6563
AclI AA'CG_TT
Cuts at: 1563 5151 5524 1563
Size: 3588 373 3063
Fragments arranged by size:
3588 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 5087 6471 6739 1632
Size: 1181 2274 1384 268 1917
Fragments arranged by size:
2274 1917 1384 1181 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6827
AflIII A'CryG_T
Cuts at: 1203 5923 6900 1203
Size: 4720 977 1327
Fragments arranged by size:
4720 1327 977
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 5753 5753
Size: 7024
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3935 4134 1184
Size: 2751 199 4074
Fragments arranged by size:
4074 2751 199
AlwNI CAG_nnn'CTG
Cuts at: 4767 6339 4767
Size: 1572 5452
ApaI G_GGCC'C
Cuts at: 3741 3741
Size: 7024
ApaLI G'TGCA_C
Cuts at: 5081 6237 5081
Size: 1156 5868
ApoI r'AATT_y
Cuts at: 1493 2513 2574 4333 4559 4711 6820 1493
Size: 1020 61 1759 226 152 2109 1697
Fragments arranged by size:
2109 1759 1697 1020 226 152 61
AseI AT'TA_AT
Cuts at: 160 1032 5578 160
Size: 872 4546 1606
Fragments arranged by size:
4546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 3076 3198 3856 4349 1870
Size: 73 1133 122 658 493 4545
Fragments arranged by size:
4545 1133 658 493 122 73
BamHI G'GATC_C
Cuts at: 3027 4354 4721 3027
Size: 1327 367 5330
Fragments arranged by size:
5330 1327 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 3714 3884 4345 5800
Size: 325 217 627 1514 413 170 461 1455
Cuts at: 5800 618
Size: 1842
Fragments arranged by size:
1842 1514 1455 627 461 413 325 217
170
BanII G_rGCy'C
Cuts at: 1391 3629 3741 3951 4343 1391
Size: 2238 112 210 392 4072
Fragments arranged by size:
4072 2238 392 210 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2367 2452 928
Size: 971 468 85 5500
Fragments arranged by size:
5500 971 468 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5215 5249 1903
Size: 34 3278 34 3678
Fragments arranged by size:
3678 3278 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4916 6126 4916
Size: 1210 5814
BclI T'GATC_A
Cuts at: 1548 2700 1548
Size: 1152 5872
BfrBI ATG'CAT
Cuts at: 2220 2220
Size: 7024
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5635 136
Size: 107 122 71 631 605 3963 1525
Fragments arranged by size:
3963 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3065 3486 7019 3065
Size: 421 3533 3070
Fragments arranged by size:
3533 3070 421
BlpI GC'TnA_GC
Cuts at: 4441 4441
Size: 7024
Bme1580I G_kGCm'C
Cuts at: 1790 3741 5085 6241 1790
Size: 1951 1344 1156 2573
Fragments arranged by size:
2573 1951 1344 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7024
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3088 3810 4755
Size: 416 53 83 399 24 1671 722 945
Cuts at: 4755 5713 442
Size: 958 1753
Fragments arranged by size:
1753 1671 958 945 722 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2553 3561 3613 3832 5684 1239
Size: 1314 1008 52 219 1852 2579
Fragments arranged by size:
2579 1852 1314 1008 219 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3161 5149 6014 6312 3161
Size: 1988 865 298 3873
Fragments arranged by size:
3873 1988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3641 5687 882
Size: 617 2142 2046 2219
Fragments arranged by size:
2219 2142 2046 617
BsaAI yAC'GTr
Cuts at: 493 1204 4074 4321 4815 493
Size: 711 2870 247 494 2702
Fragments arranged by size:
2870 2702 711 494 247
BsaBI GATnn'nnATC
Cuts at: 3531 7018 3531
Size: 3487 3537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2955 4831 5213 5848
Size: 53 83 186 936 1422 1876 382 635
Cuts at: 5848 275
Size: 1451
Fragments arranged by size:
1876 1451 1422 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3603 5457 5855 6129 6276 6687 3603
Size: 1854 398 274 147 411 3940
Fragments arranged by size:
3940 1854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3145 3175 3598 3628 1178
Size: 30 165 30 1742 30 423 30 4574
Fragments arranged by size:
4574 1742 423 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2328 2360 2915 3395 1186
Size: 1142 32 555 480 4815
Fragments arranged by size:
4815 1142 555 480 32
BseYI C'CCAG_C
Cuts at: 3908 3941 3990 4294 6227 6723 3908
Size: 33 49 304 1933 496 4209
Fragments arranged by size:
4209 1933 496 304 49 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 1163
Size: 573 516 5935
Fragments arranged by size:
5935 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4474 5235 6263 1540
Size: 275 186 2473 761 1028 2301
Fragments arranged by size:
2473 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2907 3951 4007 5085 5170 6241 2907
Size: 1044 56 1078 85 1071 3690
Fragments arranged by size:
3690 1078 1071 1044 85 56
BsmI GAATG_Cn'
Cuts at: 4108 4535 4628 4108
Size: 427 93 6504
Fragments arranged by size:
6504 427 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2946 2946
Size: 7024
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2907 3629 3741 3951 4007 4343 5085
Size: 399 1117 722 112 210 56 336 742
Cuts at: 5085 5170 6241 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1117 1071 742 722 399 336 210
112 85 56
BspEI T'CCGG_A
Cuts at: 6687 6687
Size: 7024
BspHI T'CATG_A
Cuts at: 3969 4913 6643 3969
Size: 944 1730 4350
Fragments arranged by size:
4350 1730 944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2611 3543 4102 4379 844
Size: 1767 932 559 277 3489
Fragments arranged by size:
3489 1767 932 559 277
BsrBI CCG'CTC
Cuts at: 1394 1823 2567 4911 1394
Size: 429 744 2344 3507
Fragments arranged by size:
3507 2344 744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3790 5519 5693 65
Size: 1183 2542 1729 174 1396
Fragments arranged by size:
2542 1729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3672 3902 5668 5855 1700
Size: 110 1862 230 1766 187 2869
Fragments arranged by size:
2869 1862 1766 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7024
BssSI C'ACGA_G
Cuts at: 5084 6096 5084
Size: 1012 6012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7024
BstBI TT'CG_AA
Cuts at: 2007 5828 2007
Size: 3821 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7024
BstXI CCAn_nnnn'nTGG
Cuts at: 3925 3998 4174 3925
Size: 73 176 6775
Fragments arranged by size:
6775 176 73
BstYI r'GATC_y
Cuts at: 1972 2549 2779 3027 3065 3486 4354 4721 5106
Size: 577 230 248 38 421 868 367 385
Cuts at: 5106 5123 6575 6661 6673 7019 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 868 577 421 385 367 346
248 230 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 4266 4266
Size: 7024
BtgI C'CryG_G
Cuts at: 513 1065 2022 2106 513
Size: 552 957 84 5431
Fragments arranged by size:
5431 957 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6285
Fragments arranged by size:
6285 683 56
BtsI GCAGTG_nn'
Cuts at: 3754 3943 4128 4540 5334 5354 3754
Size: 189 185 412 794 20 5424
Fragments arranged by size:
5424 794 412 189 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3396 3431 3772 3807 3814 3849 529
Size: 35 2832 35 341 35 7 35 3704
Fragments arranged by size:
3704 2832 341 35 35 35 35 7
DraI TTT'AAA
Cuts at: 4328 4328
Size: 7024
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6031 809
Size: 507 4715 1802
Fragments arranged by size:
4715 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 3110 3156
Size: 54 1425 50 275 18 537 743 46
Cuts at: 3156 3223 4471 5360 6989 8
Size: 67 1248 889 1629 43
Fragments arranged by size:
1629 1425 1248 889 743 537 275 67
54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4471 1537
Size: 275 2659 4090
Fragments arranged by size:
4090 2659 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 2892 3363 3458 4954 1353
Size: 1446 93 471 95 1496 3423
Fragments arranged by size:
3423 1496 1446 471 95 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2240 3352 5611 5985 6131 387
Size: 1064 138 651 1112 2259 374 146 1280
Fragments arranged by size:
2259 1280 1112 1064 651 374 146 138
EcoICRI GAG'CTC
Cuts at: 3949 3949
Size: 7024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2553 2813 3561 3613 3832 5087 5684
Size: 393 921 260 748 52 219 1255 597
Cuts at: 5684 6471 6739 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1255 921 787 748 597 393 268
260 219 52
EcoO109I rG'GnC_Cy
Cuts at: 3136 3737 3738 4114 3136
Size: 601 1 376 6046
Fragments arranged by size:
6046 601 376 1
EcoRI G'AATT_C
Cuts at: 4333 4711 4333
Size: 378 6646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2814 2814
Size: 7024
FspI TGC'GCA
Cuts at: 4285 5530 4285
Size: 1245 5779
FspAI rTGC'GCAy
Cuts at: 4285 4285
Size: 7024
HaeII r_GCGC'y
Cuts at: 1678 6171 1678
Size: 4493 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2800 2832 3169 3201 3276
Size: 32 135 32 1228 32 337 32 75
Cuts at: 3276 3308 3598 3630 5665 5697 5739 5771 1373
Size: 32 290 32 2035 32 42 32 2626
Fragments arranged by size:
2626 2035 1228 337 290 135 75 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2490 4368 4614 669
Size: 988 281 552 1878 246 3079
Fragments arranged by size:
3079 1878 988 552 281 246
HindIII A'AGCT_T
Cuts at: 748 3313 748
Size: 2565 4459
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KpnI G_GTAC'C
Cuts at: 3888 4349 3888
Size: 461 6563
MfeI C'AATT_G
Cuts at: 4623 4623
Size: 7024
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6138 6322 1305
Size: 4833 184 2007
Fragments arranged by size:
4833 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 3112 3225 10
Size: 54 1425 343 537 743 113 3809
Fragments arranged by size:
3809 1425 743 537 343 113 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2765 4244 4982 5341 5500 518
Size: 648 1479 120 1479 738 359 159 2042
Fragments arranged by size:
2042 1479 1479 738 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 3633 3852 3897 3925 4258 4302 4440 5117
Size: 2027 219 45 28 333 44 138 677
Cuts at: 5117 6265 6510 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 2027 1148 677 333 245 219 138
45 44 28
NaeI GCC'GGC
Cuts at: 3674 3904 3674
Size: 230 6794
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5515
Fragments arranged by size:
5515 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7024
NgoMIV G'CCGG_C
Cuts at: 3672 3902 3672
Size: 230 6794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7024
NotI GC'GGCC_GC
Cuts at: 4471 4471
Size: 7024
NsiI A_TGCA'T
Cuts at: 2222 2222
Size: 7024
NspI r_CATG'y
Cuts at: 1132 1229 2129 4382 5927 1132
Size: 97 900 2253 1545 2229
Fragments arranged by size:
2253 2229 1545 900 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7024
PciI A'CATG_T
Cuts at: 5923 5923
Size: 7024
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4031 1108
Size: 2923 4101
PfoI T'CCnGG_A
Cuts at: 2205 3458 2205
Size: 1253 5771
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 6606 6638 5093
Size: 32 1481 32 5479
Fragments arranged by size:
5479 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 4114 3136
Size: 978 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7024
PsiI TTA'TAA
Cuts at: 4594 4594
Size: 7024
PspOMI G'GGCC_C
Cuts at: 3737 3737
Size: 7024
PstI C_TGCA'G
Cuts at: 830 2608 3638 4123 4218 4376 830
Size: 1778 1030 485 95 158 3478
Fragments arranged by size:
3478 1778 1030 485 158 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 3633 3925 4302 3633
Size: 292 377 6355
Fragments arranged by size:
6355 377 292
SacI G_AGCT'C
Cuts at: 3951 3951
Size: 7024
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SbfI CC_TGCA'GG
Cuts at: 4376 4376
Size: 7024
ScaI AGT'ACT
Cuts at: 5272 6984 5272
Size: 1712 5312
SfcI C'TryA_G
Cuts at: 826 1045 2604 2771 3634 4119 4214 4372 5507
Size: 219 1559 167 863 485 95 158 1135
Cuts at: 5507 6188 6379 826
Size: 681 191 1471
Fragments arranged by size:
1559 1471 1135 863 681 485 219 191
167 158 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7024
SmaI CCC'GGG
Cuts at: 1872 4351 1872
Size: 2479 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3140 5128 6029 6291 820
Size: 197 926 1197 1988 901 262 1553
Fragments arranged by size:
1988 1553 1197 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4321 4815 493
Size: 3828 494 2702
Fragments arranged by size:
3828 2702 494
SpeI A'CTAG_T
Cuts at: 152 2227 152
Size: 2075 4949
SphI G_CATG'C
Cuts at: 1132 4382 1132
Size: 3250 3774
SspI AAT'ATT
Cuts at: 5 52 4948 5
Size: 47 4896 2081
Fragments arranged by size:
4896 2081 47
StuI AGG'CCT
Cuts at: 1517 3240 1517
Size: 1723 5301
StyI C'CwwG_G
Cuts at: 513 1065 2022 2085 2822 4463 513
Size: 552 957 63 737 1641 3074
Fragments arranged by size:
3074 1641 957 737 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 5051 5068 5221 5406 5851 325
Size: 1255 708 2763 17 153 185 445 1498
Fragments arranged by size:
2763 1498 1255 708 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4011 5270 6982
Size: 274 80 33 51 1443 2034 1259 1712
Cuts at: 6982 96
Size: 138
Fragments arranged by size:
2034 1712 1443 1259 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3145 3177 6984 7016 3145
Size: 32 3807 32 3153
Fragments arranged by size:
3807 3153 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3212 1316
Size: 1896 5128
XbaI T'CTAG_A
Cuts at: 2296 4360 2296
Size: 2064 4960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3796 4171 3796
Size: 375 6649
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7024
XmaI C'CCGG_G
Cuts at: 1870 4349 1870
Size: 2479 4545
XmnI GAAnn'nnTTC
Cuts at: 2794 5153 2794
Size: 2359 4665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4832 276
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI KasI
NarI NruI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI