Restriction map for
pFN21ASDA0093
(Circular) (Six-base) MAPSORT of: pFN21ASDA0093.seq Check: 334 from: 1 to: 7429
pFN21ASDA0093 7429 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3244 3244
Size: 7429
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5239 278
Size: 53 83 186 936 3703 2468
Fragments arranged by size:
3703 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4772 1937
Size: 2835 4594
Acc65I G'GTAC_C
Cuts at: 4750 4750
Size: 7429
AclI AA'CG_TT
Cuts at: 1563 5556 5929 1563
Size: 3993 373 3063
Fragments arranged by size:
3993 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3749 4478 4664 5492 6876 7144 1632
Size: 2117 729 186 828 1384 268 1917
Fragments arranged by size:
2117 1917 1384 828 729 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7232
AflIII A'CryG_T
Cuts at: 1203 6328 7305 1203
Size: 5125 977 1327
Fragments arranged by size:
5125 1327 977
AgeI A'CCGG_T
Cuts at: 6260 6260
Size: 7429
AhdI GACnn_n'nnGTC
Cuts at: 6158 6158
Size: 7429
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7429
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4287 1184
Size: 3103 4326
AlwNI CAG_nnn'CTG
Cuts at: 2561 2714 5172 6744 2561
Size: 153 2458 1572 3246
Fragments arranged by size:
3246 2458 1572 153
ApaLI G'TGCA_C
Cuts at: 5486 6642 5486
Size: 1156 6273
ApoI r'AATT_y
Cuts at: 1493 2071 2950 3387 3647 4320 4738 4964 5116
Size: 578 879 437 260 673 418 226 152
Cuts at: 5116 7225 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 879 673 578 437 418 260
226 152
AseI AT'TA_AT
Cuts at: 160 1032 5983 160
Size: 872 4951 1606
Fragments arranged by size:
4951 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7429
AvaI C'yCGr_G
Cuts at: 1870 1943 4515 4754 1870
Size: 73 2572 239 4545
Fragments arranged by size:
4545 2572 239 73
AvrII C'CTAG_G
Cuts at: 2329 2329
Size: 7429
BamHI G'GATC_C
Cuts at: 4759 5126 4759
Size: 367 7062
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4519 4750 6205 618
Size: 325 217 627 2732 231 1455 1842
Fragments arranged by size:
2732 1842 1455 627 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2055 2981 4615 4748 1391
Size: 664 926 1634 133 4072
Fragments arranged by size:
4072 1634 926 664 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2799 3690 928
Size: 971 900 891 4667
Fragments arranged by size:
4667 971 900 891
BbvCI CC'TCA_GC
Cuts at: 2268 2517 2268
Size: 249 7180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5620 5654 1903
Size: 34 3683 34 3678
Fragments arranged by size:
3683 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5321 6531 5321
Size: 1210 6219
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6040 136
Size: 107 122 71 631 605 4368 1525
Fragments arranged by size:
4368 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7424 7424
Size: 7429
BlpI GC'TnA_GC
Cuts at: 4846 4846
Size: 7429
Bme1580I G_kGCm'C
Cuts at: 1790 3573 5490 6646 1790
Size: 1783 1917 1156 2573
Fragments arranged by size:
2573 1917 1783 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2827 4370 5160
Size: 416 53 83 399 24 1410 1543 790
Cuts at: 5160 6118 442
Size: 958 1753
Fragments arranged by size:
1753 1543 1410 958 790 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2589 3435 3562 4405 6089 1239
Size: 839 511 846 127 843 1684 2579
Fragments arranged by size:
2579 1684 846 843 839 511 127
Bpu10I CC'TnA_GC
Cuts at: 2268 2517 2268
Size: 249 7180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3181 5554 6419 6717 3181
Size: 2373 865 298 3893
Fragments arranged by size:
3893 2373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2380 3173 6092 882
Size: 617 881 793 2919 2219
Fragments arranged by size:
2919 2219 881 793 617
BsaAI yAC'GTr
Cuts at: 493 1204 2152 4726 5220 493
Size: 711 948 2574 494 2702
Fragments arranged by size:
2702 2574 948 711 494
BsaBI GATnn'nnATC
Cuts at: 7423 7423
Size: 7429
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5236 5618 6253 275
Size: 53 83 186 936 3703 382 635 1451
Fragments arranged by size:
3703 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3693 5862 6260 6534 6681 7092 3693
Size: 2169 398 274 147 411 4030
Fragments arranged by size:
4030 2169 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7204
Fragments arranged by size:
7204 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2586 3795 1186
Size: 1400 1209 4820
Fragments arranged by size:
4820 1400 1209
BseYI C'CCAG_C
Cuts at: 2535 3261 3300 6632 7128 2535
Size: 726 39 3332 496 2836
Fragments arranged by size:
3332 2836 726 496 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6856
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4879 5640 6668 1540
Size: 275 186 2878 761 1028 2301
Fragments arranged by size:
2878 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2981 4615 5490 5575 6646 2981
Size: 1634 875 85 1071 3764
Fragments arranged by size:
3764 1634 1071 875 85
BsmI GAATG_Cn'
Cuts at: 2932 4940 5033 2932
Size: 2008 93 5328
Fragments arranged by size:
5328 2008 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2981 3573 4615 4748 5490 5575
Size: 399 265 926 592 1042 133 742 85
Cuts at: 5575 6646 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1042 926 742 592 399 265
133 85
BspEI T'CCGG_A
Cuts at: 7092 7092
Size: 7429
BspHI T'CATG_A
Cuts at: 4036 5318 7048 4036
Size: 1282 1730 4417
Fragments arranged by size:
4417 1730 1282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3244 4784 844
Size: 2400 1540 3489
Fragments arranged by size:
3489 2400 1540
BsrBI CCG'CTC
Cuts at: 1394 1823 5316 1394
Size: 429 3493 3507
Fragments arranged by size:
3507 3493 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5924 6098 65
Size: 1183 4676 174 1396
Fragments arranged by size:
4676 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2277 6073 6260 1700
Size: 110 467 3796 187 2869
Fragments arranged by size:
3796 2869 467 187 110
BsrGI T'GTAC_A
Cuts at: 96 3163 96
Size: 3067 4362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7429
BssSI C'ACGA_G
Cuts at: 5489 6501 5489
Size: 1012 6417
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2747 794
Size: 1953 5476
BstBI TT'CG_AA
Cuts at: 2007 6233 2007
Size: 4226 3203
BstEII G'GTnAC_C
Cuts at: 1184 3256 1184
Size: 2072 5357
BstXI CCAn_nnnn'nTGG
Cuts at: 3081 3081
Size: 7429
BstYI r'GATC_y
Cuts at: 1972 3373 3529 4309 4759 5126 5511 5528 6980
Size: 1401 156 780 450 367 385 17 1452
Cuts at: 6980 7066 7078 7424 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1401 780 450 385 367 346
156 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2694 4115 2694
Size: 1421 6008
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5920
Fragments arranged by size:
5920 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6690
Fragments arranged by size:
6690 683 56
BtsI GCAGTG_nn'
Cuts at: 3573 4945 5739 5759 3573
Size: 1372 794 20 5243
Fragments arranged by size:
5243 1372 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7394
DraI TTT'AAA
Cuts at: 4279 4733 4279
Size: 454 6975
DraIII CAC_nnn'GTG
Cuts at: 2747 2747
Size: 7429
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6436 809
Size: 507 5120 1802
Fragments arranged by size:
5120 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4876 5765 7394
Size: 54 1425 50 275 18 3046 889 1629
Cuts at: 7394 8
Size: 43
Fragments arranged by size:
3046 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4876 1537
Size: 275 3064 4090
Fragments arranged by size:
4090 3064 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 2992 3456 5359 1353
Size: 1257 382 464 1903 3423
Fragments arranged by size:
3423 1903 1257 464 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3983 6016 6390 6536 387
Size: 1064 138 2394 2033 374 146 1280
Fragments arranged by size:
2394 2033 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2979 4613 2979
Size: 1634 5795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2589 3435 3562 3749 4405 4478
Size: 393 446 511 846 127 187 656 73
Cuts at: 4478 4664 5492 6089 6876 7144 1239
Size: 186 828 597 787 268 1524
Fragments arranged by size:
1524 846 828 787 656 597 511 446
393 268 187 186 127 73
EcoNI CCTnn'n_nnAGG
Cuts at: 3577 3577
Size: 7429
EcoO109I rG'GnC_Cy
Cuts at: 2640 3215 3440 2640
Size: 575 225 6629
Fragments arranged by size:
6629 575 225
EcoRI G'AATT_C
Cuts at: 4738 5116 4738
Size: 378 7051
EcoRV GAT'ATC
Cuts at: 2629 2772 2629
Size: 143 7286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3305 3305
Size: 7429
FspI TGC'GCA
Cuts at: 5935 5935
Size: 7429
HaeII r_GCGC'y
Cuts at: 1678 6576 1678
Size: 4898 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2212 2244 2356 2388 2968
Size: 32 135 32 640 32 112 32 580
Cuts at: 2968 3000 3902 3934 4031 4063 6070 6102 6144
Size: 32 902 32 97 32 2007 32 42
Cuts at: 6144 6176 1373
Size: 32 2626
Fragments arranged by size:
2626 2007 902 640 580 135 112 97
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2794 4773 5019 669
Size: 988 281 856 1979 246 3079
Fragments arranged by size:
3079 1979 988 856 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7429
HpaI GTT'AAC
Cuts at: 5019 5019
Size: 7429
KpnI G_GTAC'C
Cuts at: 4754 4754
Size: 7429
MfeI C'AATT_G
Cuts at: 4689 5028 4689
Size: 339 7090
MluI A'CGCG_T
Cuts at: 7305 7305
Size: 7429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2927 3519 3957 6543 6727 1305
Size: 1622 592 438 2586 184 2007
Fragments arranged by size:
2586 2007 1622 592 438 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5607
Fragments arranged by size:
5607 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3477 4158 5387 5746 5905 518
Size: 648 2311 681 1229 359 159 2042
Fragments arranged by size:
2311 2042 1229 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3190 4845 5522 6670 6915 1606
Size: 1584 1655 677 1148 245 2120
Fragments arranged by size:
2120 1655 1584 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5920
Fragments arranged by size:
5920 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7429
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7429
NotI GC'GGCC_GC
Cuts at: 4876 4876
Size: 7429
NspI r_CATG'y
Cuts at: 1132 1229 3317 4787 6332 1132
Size: 97 2088 1470 1545 2229
Fragments arranged by size:
2229 2088 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 4702 1348
Size: 3354 4075
PciI A'CATG_T
Cuts at: 6328 6328
Size: 7429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2180 3366 3795 3893 1108
Size: 1072 1186 429 98 4644
Fragments arranged by size:
4644 1186 1072 429 98
PfoI T'CCnGG_A
Cuts at: 2055 3451 2055
Size: 1396 6033
PmeI GTTT'AAAC
Cuts at: 4733 4733
Size: 7429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3660 3692 5498 5530 7011 7043 3660
Size: 32 1806 32 1481 32 4046
Fragments arranged by size:
4046 1806 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2640 2640
Size: 7429
PshAI GACnn'nnGTC
Cuts at: 1613 4564 1613
Size: 2951 4478
PsiI TTA'TAA
Cuts at: 2828 4999 2828
Size: 2171 5258
PstI C_TGCA'G
Cuts at: 830 3214 4781 830
Size: 2384 1567 3478
Fragments arranged by size:
3478 2384 1567
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7429
PvuII CAG'CTG
Cuts at: 3190 3190
Size: 7429
SacI G_AGCT'C
Cuts at: 2981 4615 2981
Size: 1634 5795
SalI G'TCGA_C
Cuts at: 1936 4771 1936
Size: 2835 4594
SbfI CC_TGCA'GG
Cuts at: 4781 4781
Size: 7429
ScaI AGT'ACT
Cuts at: 5677 7389 5677
Size: 1712 5717
SexAI A'CCwGG_T
Cuts at: 3076 3076
Size: 7429
SfcI C'TryA_G
Cuts at: 826 1045 3210 3875 4414 4777 5912 6593 6784
Size: 219 2165 665 539 363 1135 681 191
Cuts at: 6784 826
Size: 1471
Fragments arranged by size:
2165 1471 1135 681 665 539 363 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7429
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7429
SmaI CCC'GGG
Cuts at: 1872 4756 1872
Size: 2884 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3196 5533 6434 6696 820
Size: 197 926 1253 2337 901 262 1553
Fragments arranged by size:
2337 1553 1253 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4726 5220 493
Size: 4233 494 2702
Fragments arranged by size:
4233 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7429
SphI G_CATG'C
Cuts at: 1132 3317 4787 1132
Size: 2185 1470 3774
Fragments arranged by size:
3774 2185 1470
SspI AAT'ATT
Cuts at: 5 52 2250 3851 4064 5353 5
Size: 47 2198 1601 213 1289 2081
Fragments arranged by size:
2198 2081 1601 1289 213 47
StuI AGG'CCT
Cuts at: 1517 2112 2864 3331 1517
Size: 595 752 467 5615
Fragments arranged by size:
5615 752 595 467
StyI C'CwwG_G
Cuts at: 513 1065 2022 2329 2632 4868 513
Size: 552 957 307 303 2236 3074
Fragments arranged by size:
3074 2236 957 552 307 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 4715 5456 5473 5626 5811 6256
Size: 1255 2115 1020 741 17 153 185 445
Cuts at: 6256 325
Size: 1498
Fragments arranged by size:
2115 1498 1255 1020 741 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3163 5675 7387
Size: 274 80 33 51 1443 1186 2512 1712
Cuts at: 7387 96
Size: 138
Fragments arranged by size:
2512 1712 1443 1186 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3552 3584 7389 7421 3552
Size: 32 3805 32 3560
Fragments arranged by size:
3805 3560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7429
XbaI T'CTAG_A
Cuts at: 2663 4765 2663
Size: 2102 5327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4709 4709
Size: 7429
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7429
XmaI C'CCGG_G
Cuts at: 1870 4754 1870
Size: 2884 4545
XmnI GAAnn'nnTTC
Cuts at: 5558 5558
Size: 7429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5237 276
Size: 53 83 186 936 3703 2468
Fragments arranged by size:
3703 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI