Restriction map for
pFN21ASDA0084
(Circular) (Six-base) MAPSORT of: pFN21ASDA0084.seq Check: 4668 from: 1 to: 6463
pFN21ASDA0084 6463 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2558 3322 3806 1937
Size: 621 764 484 4594
Fragments arranged by size:
4594 764 621 484
Acc65I G'GTAC_C
Cuts at: 3784 3784
Size: 6463
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4526 5910 6178 1632
Size: 1661 1233 1384 268 1917
Fragments arranged by size:
1917 1661 1384 1233 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6266
AflIII A'CryG_T
Cuts at: 1203 2613 5362 6339 1203
Size: 1410 2749 977 1327
Fragments arranged by size:
2749 1410 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 3239 1166
Size: 2073 4390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6463
AlwNI CAG_nnn'CTG
Cuts at: 2230 2488 4206 5778 2230
Size: 258 1718 1572 2915
Fragments arranged by size:
2915 1718 1572 258
ApaI G_GGCC'C
Cuts at: 3554 3661 3554
Size: 107 6356
ApaLI G'TGCA_C
Cuts at: 4520 5676 4520
Size: 1156 5307
ApoI r'AATT_y
Cuts at: 1493 2492 3772 3998 4150 6259 1493
Size: 999 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 999 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 3788 1870
Size: 73 1845 4545
Fragments arranged by size:
4545 1845 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2498 2531 2498
Size: 33 6430
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2713 3784 5239 618
Size: 325 217 627 926 1071 1455 1842
Fragments arranged by size:
1842 1455 1071 926 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2175 2691 3554 3661 3782 1391
Size: 784 516 863 107 121 4072
Fragments arranged by size:
4072 863 784 516 121 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2309 3022 3468 3474 928
Size: 971 410 713 446 6 3917
Fragments arranged by size:
3917 971 713 446 410 6
BbvCI CC'TCA_GC
Cuts at: 2185 3720 2185
Size: 1535 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4654 4688 1903
Size: 34 2717 34 3678
Fragments arranged by size:
3678 2717 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3295 4355 5565 3295
Size: 1060 1210 4193
Fragments arranged by size:
4193 1210 1060
BclI T'GATC_A
Cuts at: 1548 2337 1548
Size: 789 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5074 136
Size: 107 122 71 631 605 3402 1525
Fragments arranged by size:
3402 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2354 2856 6458 2354
Size: 502 3602 2359
Fragments arranged by size:
3602 2359 502
BlpI GC'TnA_GC
Cuts at: 3880 3880
Size: 6463
Bme1580I G_kGCm'C
Cuts at: 1790 2718 3198 3554 3661 3684 4524 5680 1790
Size: 928 480 356 107 23 840 1156 2573
Fragments arranged by size:
2573 1156 928 840 480 356 107 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 3687 4194
Size: 416 53 83 399 24 1235 1035 507
Cuts at: 4194 5152 442
Size: 958 1753
Fragments arranged by size:
1753 1235 1035 958 507 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2857 2710
Size: 147 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2252 2333 2642 3355 3507 3714 5123
Size: 926 87 81 309 713 152 207 1409
Cuts at: 5123 1239
Size: 2579
Fragments arranged by size:
2579 1409 926 713 309 207 152 87
81
Bpu10I CC'TnA_GC
Cuts at: 2185 2695 3720 2185
Size: 510 1025 4928
Fragments arranged by size:
4928 1025 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4588 5453 5751 4588
Size: 865 298 5300
Fragments arranged by size:
5300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3307 5126 882
Size: 617 1808 1819 2219
Fragments arranged by size:
2219 1819 1808 617
BsaAI yAC'GTr
Cuts at: 493 1204 2603 3193 3760 4254 493
Size: 711 1399 590 567 494 2702
Fragments arranged by size:
2702 1399 711 590 567 494
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2306 2967 4270 4652
Size: 53 83 186 936 773 661 1303 382
Cuts at: 4652 5287 275
Size: 635 1451
Fragments arranged by size:
1451 1303 936 773 661 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2551 2744 2803 3000 4896 5294 5568 5715 6126
Size: 193 59 197 1896 398 274 147 411
Cuts at: 6126 2551
Size: 2888
Fragments arranged by size:
2888 1896 411 398 274 197 193 147
59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6238
Fragments arranged by size:
6238 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3554 1186
Size: 2368 4095
BseYI C'CCAG_C
Cuts at: 2223 2666 2690 2732 5666 6162 2223
Size: 443 24 42 2934 496 2524
Fragments arranged by size:
2934 2524 496 443 42 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 2300 2674 3200 3650 1163
Size: 573 516 48 374 526 450 3976
Fragments arranged by size:
3976 573 526 516 450 374 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2319 2551 2662 2985 4524 4609 5680 2175
Size: 144 232 111 323 1539 85 1071 2958
Fragments arranged by size:
2958 1539 1071 323 232 144 111 85
BsmI GAATG_Cn'
Cuts at: 3974 4067 3974
Size: 93 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2319 2551 2662 2691 2718 2985
Size: 399 385 144 232 111 29 27 267
Cuts at: 2985 3198 3554 3661 3684 3782 4524 4609 5680
Size: 213 356 107 23 98 742 85 1071
Cuts at: 5680 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 385 356 267 232
213 144 111 107 98 85 29 27
23
BspEI T'CCGG_A
Cuts at: 2744 3000 6126 2744
Size: 256 3126 3081
Fragments arranged by size:
3126 3081 256
BspHI T'CATG_A
Cuts at: 4352 6082 4352
Size: 1730 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 3818 844
Size: 1379 1595 3489
Fragments arranged by size:
3489 1595 1379
BsrBI CCG'CTC
Cuts at: 1394 1823 2296 2958 4350 1394
Size: 429 473 662 1392 3507
Fragments arranged by size:
3507 1392 662 473 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4958 5132 65
Size: 1183 3710 174 1396
Fragments arranged by size:
3710 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2898 5107 5294 1700
Size: 110 1088 2209 187 2869
Fragments arranged by size:
2869 2209 1088 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6463
BstBI TT'CG_AA
Cuts at: 2007 5267 2007
Size: 3260 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 2354 2463 2856 3793 4160 4545 4562 6014
Size: 382 109 393 937 367 385 17 1452
Cuts at: 6014 6100 6112 6458 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 937 393 385 382 367 346
109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2100 2100
Size: 6463
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4954
Fragments arranged by size:
4954 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 2196 2551 2662 3979 4773 4793 2196
Size: 355 111 1317 794 20 3866
Fragments arranged by size:
3866 1317 794 355 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3910 4799
Size: 54 1425 50 275 18 1114 966 889
Cuts at: 4799 6428 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1114 966 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2826 2876 4393 1353
Size: 734 739 50 1517 3423
Fragments arranged by size:
3423 1517 739 734 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5050 5424 5570 387
Size: 1064 138 3461 374 146 1280
Fragments arranged by size:
3461 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2173 2173
Size: 6463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2252 2333 2642 3293 3355 3507
Size: 393 533 87 81 309 651 62 152
Cuts at: 3507 3714 4526 5123 5910 6178 1239
Size: 207 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 651 597 533 393 309
268 207 152 87 81 62
EcoO109I rG'GnC_Cy
Cuts at: 2181 2485 2699 3550 3551 3657 3658 2181
Size: 304 214 851 1 106 1 4986
Fragments arranged by size:
4986 851 304 214 106 1 1
EcoRI G'AATT_C
Cuts at: 3772 4150 3772
Size: 378 6085
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 5610 1678
Size: 3932 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3069 3101 3304
Size: 32 135 32 1028 32 437 32 203
Cuts at: 3304 3336 5104 5136 5178 5210 1373
Size: 32 1768 32 42 32 2626
Fragments arranged by size:
2626 1768 1028 437 203 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3807 4053 669
Size: 988 281 1869 246 3079
Fragments arranged by size:
3079 1869 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KpnI G_GTAC'C
Cuts at: 3788 3788
Size: 6463
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 2613 6339 2613
Size: 3726 2737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5577 5761 1305
Size: 4272 184 2007
Fragments arranged by size:
4272 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2946 10
Size: 54 1425 343 1114 3527
Fragments arranged by size:
3527 1425 1114 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3239 4421 4780 4939 518
Size: 648 1851 222 1182 359 159 2042
Fragments arranged by size:
2042 1851 1182 648 359 222 159
MspA1I CmG'CkG
Cuts at: 1606 3580 3879 4556 5704 5949 1606
Size: 1974 299 677 1148 245 2120
Fragments arranged by size:
2120 1974 1148 677 299 245
NaeI GCC'GGC
Cuts at: 2900 2900
Size: 6463
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4954
Fragments arranged by size:
4954 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NgoMIV G'CCGG_C
Cuts at: 2898 2898
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 3016 3821 5366 1132
Size: 97 1787 805 1545 2229
Fragments arranged by size:
2229 1787 1545 805 97
PasI CC'CwG_GG
Cuts at: 1348 2177 1348
Size: 829 5634
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2230 1108
Size: 1122 5341
PfoI T'CCnGG_A
Cuts at: 2142 2229 2466 3162 3691 2142
Size: 87 237 696 529 4914
Fragments arranged by size:
4914 696 529 237 87
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PmlI CAC'GTG
Cuts at: 2603 2603
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 6045 6077 4532
Size: 32 1481 32 4918
Fragments arranged by size:
4918 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2181 2181
Size: 6463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 4033 4033
Size: 6463
PspOMI G'GGCC_C
Cuts at: 3550 3657 3550
Size: 107 6356
PstI C_TGCA'G
Cuts at: 830 3815 830
Size: 2985 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
PvuII CAG'CTG
Cuts at: 3580 3580
Size: 6463
SacI G_AGCT'C
Cuts at: 2175 2175
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SapI GCTCTTCn'nnn_
Cuts at: 2826 2876 2826
Size: 50 6413
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SfcI C'TryA_G
Cuts at: 826 1045 3811 4946 5627 5818 826
Size: 219 2766 1135 681 191 1471
Fragments arranged by size:
2766 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4567 5468 5730 820
Size: 197 926 2624 901 262 1553
Fragments arranged by size:
2624 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3760 4254 493
Size: 3267 494 2702
Fragments arranged by size:
3267 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 3016 3821 1132
Size: 1884 805 3774
Fragments arranged by size:
3774 1884 805
SspI AAT'ATT
Cuts at: 5 52 2077 4387 5
Size: 47 2025 2310 2081
Fragments arranged by size:
2310 2081 2025 47
StuI AGG'CCT
Cuts at: 1517 2098 1517
Size: 581 5882
StyI C'CwwG_G
Cuts at: 513 1065 2022 2817 3037 3902 513
Size: 552 957 795 220 865 3074
Fragments arranged by size:
3074 957 865 795 552 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 3599 4490 4507 4660 4845 5290
Size: 1255 723 1296 891 17 153 185 445
Cuts at: 5290 325
Size: 1498
Fragments arranged by size:
1498 1296 1255 891 723 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4709 6421 96
Size: 274 80 33 51 1443 2732 1712 138
Fragments arranged by size:
2732 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6423 6455 6423
Size: 32 6431
Tth111I GACn'n_nGTC
Cuts at: 1316 3318 1316
Size: 2002 4461
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6463
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 4592 4592
Size: 6463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI