Restriction map for
pFN21ASDA0083
(Circular) (Six-base) MAPSORT of: pFN21ASDA0083.seq Check: 7266 from: 1 to: 7909
pFN21ASDA0083 7909 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3554 3554
Size: 7909
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5020 5719 278
Size: 53 83 186 936 3484 699 2468
Fragments arranged by size:
3484 2468 936 699 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4920 5252 1937
Size: 2983 332 4594
Fragments arranged by size:
4594 2983 332
Acc65I G'GTAC_C
Cuts at: 2160 5230 2160
Size: 3070 4839
AclI AA'CG_TT
Cuts at: 1563 6036 6409 1563
Size: 4473 373 3063
Fragments arranged by size:
4473 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3341 5972 7356 7624 1632
Size: 1709 2631 1384 268 1917
Fragments arranged by size:
2631 1917 1709 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 4528 820
Size: 197 3511 4201
Fragments arranged by size:
4201 3511 197
AflIII A'CryG_T
Cuts at: 1203 2771 3481 3774 4247 6808 7785 1203
Size: 1568 710 293 473 2561 977 1327
Fragments arranged by size:
2561 1568 1327 977 710 473 293
AgeI A'CCGG_T
Cuts at: 2157 6740 2157
Size: 4583 3326
AhdI GACnn_n'nnGTC
Cuts at: 6638 6638
Size: 7909
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7909
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 2247
Size: 32 7877
AlwNI CAG_nnn'CTG
Cuts at: 3011 3319 5652 7224 3011
Size: 308 2333 1572 3696
Fragments arranged by size:
3696 2333 1572 308
ApaI G_GGCC'C
Cuts at: 4346 4346
Size: 7909
ApaLI G'TGCA_C
Cuts at: 5966 7122 5966
Size: 1156 6753
ApoI r'AATT_y
Cuts at: 1493 2938 4011 4170 4284 4634 5218 5444 5596
Size: 1445 1073 159 114 350 584 226 152
Cuts at: 5596 7705 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1445 1073 584 350 226 159
152 114
AseI AT'TA_AT
Cuts at: 160 1032 5135 6463 160
Size: 872 4103 1328 1606
Fragments arranged by size:
4103 1606 1328 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 5234 1870
Size: 73 3291 4545
Fragments arranged by size:
4545 3291 73
AvrII C'CTAG_G
Cuts at: 2825 2825
Size: 7909
BamHI G'GATC_C
Cuts at: 5239 5606 5239
Size: 367 7542
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2160 2402 3536 5230 6685
Size: 325 217 627 373 242 1134 1694 1455
Cuts at: 6685 618
Size: 1842
Fragments arranged by size:
1842 1694 1455 1134 627 373 325 242
217
BanII G_rGCy'C
Cuts at: 1391 2468 4346 4429 5228 1391
Size: 1077 1878 83 799 4072
Fragments arranged by size:
4072 1878 1077 799 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3158 928
Size: 971 1259 5679
Fragments arranged by size:
5679 1259 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6100 6134 1903
Size: 34 4163 34 3678
Fragments arranged by size:
4163 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2391 3865 5801 7011 2391
Size: 1474 1936 1210 3289
Fragments arranged by size:
3289 1936 1474 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7909
BfrBI ATG'CAT
Cuts at: 2262 2698 2262
Size: 436 7473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3913 6520 136
Size: 107 122 71 631 605 2241 2607 1525
Fragments arranged by size:
2607 2241 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7904 7904
Size: 7909
BlpI GC'TnA_GC
Cuts at: 5326 5326
Size: 7909
Bme1580I G_kGCm'C
Cuts at: 1790 4346 5076 5970 7126 1790
Size: 2556 730 894 1156 2573
Fragments arranged by size:
2573 2556 1156 894 730
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5192 5640 6598
Size: 416 53 83 399 24 3775 448 958
Cuts at: 6598 442
Size: 1753
Fragments arranged by size:
3775 1753 958 448 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 3392 4481 4580 6569 1239
Size: 824 1329 1089 99 1989 2579
Fragments arranged by size:
2579 1989 1329 1089 824 99
Bpu10I CC'TnA_GC
Cuts at: 4198 4198
Size: 7909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3014 6034 6899 7197 3014
Size: 3020 865 298 3726
Fragments arranged by size:
3726 3020 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6572 882
Size: 617 5073 2219
Fragments arranged by size:
5073 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5206 5700 493
Size: 711 4002 494 2702
Fragments arranged by size:
4002 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7903 7903
Size: 7909
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5017 5716 6098 6733
Size: 53 83 186 936 3484 699 382 635
Cuts at: 6733 275
Size: 1451
Fragments arranged by size:
3484 1451 936 699 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2157 6342 6740 7014 7161 7572 2157
Size: 4185 398 274 147 411 2494
Fragments arranged by size:
4185 2494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7684
Fragments arranged by size:
7684 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 1186
Size: 901 7008
BseYI C'CCAG_C
Cuts at: 3312 7112 7608 3312
Size: 3800 496 3613
Fragments arranged by size:
3800 3613 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2314 4517 1163
Size: 573 578 2203 4555
Fragments arranged by size:
4555 2203 578 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5359 6120 7148 1540
Size: 275 186 3358 761 1028 2301
Fragments arranged by size:
3358 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2468 3232 4429 5970 6055 7126 2144
Size: 324 764 1197 1541 85 1071 2927
Fragments arranged by size:
2927 1541 1197 1071 764 324 85
BsmI GAATG_Cn'
Cuts at: 3107 3885 5420 5513 3107
Size: 778 1535 93 5503
Fragments arranged by size:
5503 1535 778 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2468 3232 4346 4429 5076 5228
Size: 399 354 324 764 1114 83 647 152
Cuts at: 5228 5970 6055 7126 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1114 1071 764 742 647 399 354
324 152 85 83
BspEI T'CCGG_A
Cuts at: 7572 7572
Size: 7909
BspHI T'CATG_A
Cuts at: 5798 7528 5798
Size: 1730 6179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3554 5264 844
Size: 2710 1710 3489
Fragments arranged by size:
3489 2710 1710
BsrBI CCG'CTC
Cuts at: 1394 1823 5796 1394
Size: 429 3973 3507
Fragments arranged by size:
3973 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4765 6404 6578 65
Size: 1183 3517 1639 174 1396
Fragments arranged by size:
3517 1639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 2157 6553 6740 1700
Size: 110 276 71 4396 187 2869
Fragments arranged by size:
4396 2869 276 187 110 71
BsrGI T'GTAC_A
Cuts at: 96 2185 2970 4483 96
Size: 2089 785 1513 3522
Fragments arranged by size:
3522 2089 1513 785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7909
BssSI C'ACGA_G
Cuts at: 5969 6981 5969
Size: 1012 6897
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7909
BstBI TT'CG_AA
Cuts at: 2007 6713 2007
Size: 4706 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7909
BstXI CCAn_nnnn'nTGG
Cuts at: 3766 3766
Size: 7909
BstYI r'GATC_y
Cuts at: 1972 5239 5606 5991 6008 7460 7546 7558 7904
Size: 3267 367 385 17 1452 86 12 346
Cuts at: 7904 1972
Size: 1977
Fragments arranged by size:
3267 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2267 2267
Size: 7909
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6400
Fragments arranged by size:
6400 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3935 487
Size: 683 56 2709 4461
Fragments arranged by size:
4461 2709 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 4596 5425 6219 6239 2226
Size: 2370 829 794 20 3896
Fragments arranged by size:
3896 2370 829 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7874
DraI TTT'AAA
Cuts at: 4099 4612 5121 5213 4099
Size: 513 509 92 6795
Fragments arranged by size:
6795 513 509 92
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6916 809
Size: 507 5600 1802
Fragments arranged by size:
5600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4581 5356 6245
Size: 54 1425 50 275 18 2751 775 889
Cuts at: 6245 7874 8
Size: 1629 43
Fragments arranged by size:
2751 1629 1425 889 775 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5356 1537
Size: 275 3544 4090
Fragments arranged by size:
4090 3544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2431 2989 3183 3437 4209 5839 1353
Size: 701 377 558 194 254 772 1630 3423
Fragments arranged by size:
3423 1630 772 701 558 377 254 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 2105 6496 6870 7016 387
Size: 1064 138 504 12 4391 374 146 1280
Fragments arranged by size:
4391 1280 1064 504 374 146 138 12
EcoICRI GAG'CTC
Cuts at: 2466 4427 2466
Size: 1961 5948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 3341 3392 4481 4580 5972 6569
Size: 393 431 1278 51 1089 99 1392 597
Cuts at: 6569 7356 7624 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1392 1278 1089 787 597 431 393
268 99 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3975 3975
Size: 7909
EcoO109I rG'GnC_Cy
Cuts at: 4343 4343
Size: 7909
EcoRI G'AATT_C
Cuts at: 4011 5218 5596 4011
Size: 1207 378 6324
Fragments arranged by size:
6324 1207 378
EcoRV GAT'ATC
Cuts at: 2308 2308
Size: 7909
FspI TGC'GCA
Cuts at: 6415 6415
Size: 7909
HaeII r_GCGC'y
Cuts at: 1678 2218 4730 7056 1678
Size: 540 2512 2326 2531
Fragments arranged by size:
2531 2512 2326 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 2365 2397 2455
Size: 32 135 32 746 32 15 32 58
Cuts at: 2455 2487 3365 3397 3985 4017 4298 4330 4553
Size: 32 878 32 588 32 281 32 223
Cuts at: 4553 4585 5135 5167 6550 6582 6624 6656 1373
Size: 32 550 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 878 746 588 550 281 223
135 58 42 32 32 32 32 32
32 32 32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 4044 4069 4229 4491 5253
Size: 988 281 1428 678 25 160 262 762
Cuts at: 5253 5499 669
Size: 246 3079
Fragments arranged by size:
3079 1428 988 762 678 281 262 246
160 25
HindIII A'AGCT_T
Cuts at: 748 4444 748
Size: 3696 4213
HpaI GTT'AAC
Cuts at: 3366 4491 5499 3366
Size: 1125 1008 5776
Fragments arranged by size:
5776 1125 1008
KpnI G_GTAC'C
Cuts at: 2164 5234 2164
Size: 3070 4839
MfeI C'AATT_G
Cuts at: 3223 3279 5508 3223
Size: 56 2229 5624
Fragments arranged by size:
5624 2229 56
MluI A'CGCG_T
Cuts at: 7785 7785
Size: 7909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7023 7207 1305
Size: 5718 184 2007
Fragments arranged by size:
5718 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4583 10
Size: 54 1425 343 2751 3336
Fragments arranged by size:
3336 2751 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 5867 6226 6385 518
Size: 648 981 3720 359 159 2042
Fragments arranged by size:
3720 2042 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2911 5325 6002 7150 7395 1606
Size: 426 879 2414 677 1148 245 2120
Fragments arranged by size:
2414 2120 1148 879 677 426 245
NaeI GCC'GGC
Cuts at: 2088 2088
Size: 7909
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6400
Fragments arranged by size:
6400 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7909
NgoMIV G'CCGG_C
Cuts at: 2086 2086
Size: 7909
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7909
NotI GC'GGCC_GC
Cuts at: 5356 5356
Size: 7909
NsiI A_TGCA'T
Cuts at: 2264 2700 2264
Size: 436 7473
NspI r_CATG'y
Cuts at: 1132 1229 2392 2775 3485 4251 5267 6812 1132
Size: 97 1163 383 710 766 1016 1545 2229
Fragments arranged by size:
2229 1545 1163 1016 766 710 383 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7909
PciI A'CATG_T
Cuts at: 2771 3481 4247 6808 2771
Size: 710 766 2561 3872
Fragments arranged by size:
3872 2561 766 710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3011 5182 1108
Size: 1903 2171 3835
Fragments arranged by size:
3835 2171 1903
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5978 6010 7491 7523 5978
Size: 32 1481 32 6364
Fragments arranged by size:
6364 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7909
PsiI TTA'TAA
Cuts at: 5479 5479
Size: 7909
PspOMI G'GGCC_C
Cuts at: 4342 4342
Size: 7909
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3193 3225 3193
Size: 32 7877
PstI C_TGCA'G
Cuts at: 830 5261 830
Size: 4431 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
PvuII CAG'CTG
Cuts at: 2032 2032
Size: 7909
SacI G_AGCT'C
Cuts at: 2468 4429 2468
Size: 1961 5948
SalI G'TCGA_C
Cuts at: 1936 5251 1936
Size: 3315 4594
SapI GCTCTTCn'nnn_
Cuts at: 2431 2989 4209 2431
Size: 558 1220 6131
Fragments arranged by size:
6131 1220 558
SbfI CC_TGCA'GG
Cuts at: 5261 5261
Size: 7909
ScaI AGT'ACT
Cuts at: 6157 7869 6157
Size: 1712 6197
SexAI A'CCwGG_T
Cuts at: 2163 2163
Size: 7909
SfcI C'TryA_G
Cuts at: 826 1045 5257 6392 7073 7264 826
Size: 219 4212 1135 681 191 1471
Fragments arranged by size:
4212 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 5236 1872
Size: 3364 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3029 4528 6013 6914 7176 820
Size: 197 926 1086 1499 1485 901 262 1553
Fragments arranged by size:
1553 1499 1485 1086 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5206 5700 493
Size: 4713 494 2702
Fragments arranged by size:
4713 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 5267 1132
Size: 4135 3774
SspI AAT'ATT
Cuts at: 5 52 3206 3341 3509 4075 4272 5833 5
Size: 47 3154 135 168 566 197 1561 2081
Fragments arranged by size:
3154 2081 1561 566 197 168 135 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7909
StyI C'CwwG_G
Cuts at: 513 1065 2022 2825 4661 4930 5348 513
Size: 552 957 803 1836 269 418 3074
Fragments arranged by size:
3074 1836 957 803 552 418 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3667 5174 5936 5953 6106 6291 6736
Size: 1255 2087 1507 762 17 153 185 445
Cuts at: 6736 325
Size: 1498
Fragments arranged by size:
2087 1507 1498 1255 762 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2185 2970 3998
Size: 274 80 33 51 1443 208 785 1028
Cuts at: 3998 4483 4500 6155 7867 96
Size: 485 17 1655 1712 138
Fragments arranged by size:
1712 1655 1443 1028 785 485 274 208
138 80 51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7869 7901 7869
Size: 32 7877
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7909
XbaI T'CTAG_A
Cuts at: 3067 3614 4207 5245 3067
Size: 547 593 1038 5731
Fragments arranged by size:
5731 1038 593 547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2492 3530 2492
Size: 1038 6871
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7909
XmaI C'CCGG_G
Cuts at: 1870 5234 1870
Size: 3364 4545
XmnI GAAnn'nnTTC
Cuts at: 3754 6038 3754
Size: 2284 5625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5018 5717 276
Size: 53 83 186 936 3484 699 2468
Fragments arranged by size:
3484 2468 936 699 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI
BstZ17I DraIII FalI FseI FspAI KasI NarI NruI
PacI PfoI PmlI PpuMI PspXI RsrII SacII SanDI
SfiI SfoI SrfI SwaI