Restriction map for
pFN21ASDA0076
(Circular) (Six-base) MAPSORT of: pFN21ASDA0076.seq Check: 7894 from: 1 to: 9823
pFN21ASDA0076 9823 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5325 5473 5325
Size: 148 9675
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6885 7633 278
Size: 53 83 186 936 5349 748 2468
Fragments arranged by size:
5349 2468 936 748 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3714 5982 7166 1937
Size: 1777 2268 1184 4594
Fragments arranged by size:
4594 2268 1777 1184
Acc65I G'GTAC_C
Cuts at: 7144 7144
Size: 9823
AclI AA'CG_TT
Cuts at: 1563 7950 8323 1563
Size: 6387 373 3063
Fragments arranged by size:
6387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3058 3753 4112 5660 6479 7886 9270 9538
Size: 1426 695 359 1548 819 1407 1384 268
Cuts at: 9538 1632
Size: 1917
Fragments arranged by size:
1917 1548 1426 1407 1384 819 695 359
268
AfeI AGC'GCT
Cuts at: 4874 4874
Size: 9823
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9626
AflIII A'CryG_T
Cuts at: 1203 2526 8722 9699 1203
Size: 1323 6196 977 1327
Fragments arranged by size:
6196 1327 1323 977
AgeI A'CCGG_T
Cuts at: 8654 8654
Size: 9823
AhdI GACnn_n'nnGTC
Cuts at: 8552 8552
Size: 9823
AleI CACnn'nnGTG
Cuts at: 1166 5670 1166
Size: 4504 5319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5109 1184
Size: 3925 5898
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 4334 4366 5171 5203 6281 6313 6995
Size: 32 1411 32 805 32 1078 32 682
Cuts at: 6995 7027 2891
Size: 32 5687
Fragments arranged by size:
5687 1411 1078 805 682 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 2676 3892 4039 4204 4336 5239 5944 5998 6258
Size: 1216 147 165 132 903 705 54 260
Cuts at: 6258 6409 6952 6968 7566 9138 2676
Size: 151 543 16 598 1572 3361
Fragments arranged by size:
3361 1572 1216 903 705 598 543 260
165 151 147 132 54 16
ApaI G_GGCC'C
Cuts at: 2070 2295 2326 6534 6821 2070
Size: 225 31 4208 287 5072
Fragments arranged by size:
5072 4208 287 225 31
ApaLI G'TGCA_C
Cuts at: 3290 7880 9036 3290
Size: 4590 1156 4077
Fragments arranged by size:
4590 4077 1156
ApoI r'AATT_y
Cuts at: 1493 2048 5089 7132 7358 7510 9619 1493
Size: 555 3041 2043 226 152 2109 1697
Fragments arranged by size:
3041 2109 2043 1697 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8377 160
Size: 872 7345 1606
Fragments arranged by size:
7345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9823
AvaI C'yCGr_G
Cuts at: 1870 1943 4112 4714 4731 5418 5589 5863 7062
Size: 73 2169 602 17 687 171 274 1199
Cuts at: 7062 7148 1870
Size: 86 4545
Fragments arranged by size:
4545 2169 1199 687 602 274 171 86
73 17
AvrII C'CTAG_G
Cuts at: 4921 4921
Size: 9823
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6428 6461 6428
Size: 33 9790
BamHI G'GATC_C
Cuts at: 7153 7520 7153
Size: 367 9456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2924 5344 7144 8599
Size: 325 217 627 269 868 2420 1800 1455
Cuts at: 8599 618
Size: 1842
Fragments arranged by size:
2420 1842 1800 1455 868 627 325 269
217
BanII G_rGCy'C
Cuts at: 1391 2070 2186 2295 2326 2386 2478 2851 2990
Size: 679 116 109 31 60 92 373 139
Cuts at: 2990 3041 3048 3397 4514 5337 5408 5731 6501
Size: 51 7 349 1117 823 71 323 770
Cuts at: 6501 6534 6821 7142 1391
Size: 33 287 321 4072
Fragments arranged by size:
4072 1117 823 770 679 373 349 323
321 287 139 116 109 92 71 60
51 33 31 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3333 4446 5251 6693 928
Size: 971 1434 1113 805 1442 4058
Fragments arranged by size:
4058 1442 1434 1113 971 805
BbvCI CC'TCA_GC
Cuts at: 3021 3947 6678 3021
Size: 926 2731 6166
Fragments arranged by size:
6166 2731 926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2816 2850 4705 4739 4949 4983 5701
Size: 34 879 34 1855 34 210 34 718
Cuts at: 5701 5735 8014 8048 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 1855 879 718 210 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6019 7715 8925 6019
Size: 1696 1210 6917
Fragments arranged by size:
6917 1696 1210
BclI T'GATC_A
Cuts at: 1548 3699 1548
Size: 2151 7672
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2065 3406 4157
Size: 107 122 71 631 605 393 1341 751
Cuts at: 4157 8434 136
Size: 4277 1525
Fragments arranged by size:
4277 1525 1341 751 631 605 393 122
107 71
BglII A'GATC_T
Cuts at: 2241 3309 9818 2241
Size: 1068 6509 2246
Fragments arranged by size:
6509 2246 1068
BlpI GC'TnA_GC
Cuts at: 2208 2453 2989 3063 6384 7240 2208
Size: 245 536 74 3321 856 4791
Fragments arranged by size:
4791 3321 856 536 245 74
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2070 2295 2326 2414 2927 3294 3630
Size: 271 9 225 31 88 513 367 336
Cuts at: 3630 3688 5002 6284 6534 6821 6920 7074 7884
Size: 58 1314 1282 250 287 99 154 810
Cuts at: 7884 9040 1790
Size: 1156 2573
Fragments arranged by size:
2573 1314 1282 1156 810 513 367 336
287 271 250 225 154 99 88 58
31 9
BmgBI CAC'GTC
Cuts at: 1318 5980 6369 1318
Size: 4662 389 4772
Fragments arranged by size:
4772 4662 389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7554 8512 442
Size: 416 53 83 399 24 6137 958 1753
Fragments arranged by size:
6137 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2690 1056
Size: 1634 8189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4579 6361 6611 4211
Size: 368 1782 250 7423
Fragments arranged by size:
7423 1782 368 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2417 2505 2578 2918 2924 3326 3433
Size: 1124 54 88 73 340 6 402 107
Cuts at: 3433 3545 3554 4181 4340 4498 4847 5029 5261
Size: 112 9 627 159 158 349 182 232
Cuts at: 5261 5422 5894 5909 5975 6041 6821 8483 1239
Size: 161 472 15 66 66 780 1662 2579
Fragments arranged by size:
2579 1662 1124 780 627 472 402 349
340 232 182 161 159 158 112 107
88 73 66 66 54 15 9 6
Bpu10I CC'TnA_GC
Cuts at: 3021 3816 3947 4329 5945 6678 3021
Size: 795 131 382 1616 733 6166
Fragments arranged by size:
6166 1616 795 733 382 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5551 6356 6581 6731 7948 8813 9111 5551
Size: 805 225 150 1217 865 298 6263
Fragments arranged by size:
6263 1217 865 805 298 225 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5866 6472 8486 882
Size: 617 4367 606 2014 2219
Fragments arranged by size:
4367 2219 2014 617 606
BsaAI yAC'GTr
Cuts at: 493 1204 2449 5668 7120 7614 493
Size: 711 1245 3219 1452 494 2702
Fragments arranged by size:
3219 2702 1452 1245 711 494
BsaBI GATnn'nnATC
Cuts at: 2124 9817 2124
Size: 7693 2130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6882 6928 7630 8012
Size: 53 83 186 936 5349 46 702 382
Cuts at: 8012 8647 275
Size: 635 1451
Fragments arranged by size:
5349 1451 936 702 635 382 186 83
53 46
BsaWI w'CCGG_w
Cuts at: 4048 7113 8256 8654 8928 9075 9486 4048
Size: 3065 1143 398 274 147 411 4385
Fragments arranged by size:
4385 3065 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2404 2434 2848 2878 2891
Size: 30 165 30 1001 30 414 30 13
Cuts at: 2891 2921 4110 4140 4271 4301 4523 4553 5171
Size: 30 1189 30 131 30 222 30 618
Cuts at: 5171 5201 5390 5420 6281 6311 6872 6902 6995
Size: 30 189 30 861 30 561 30 93
Cuts at: 6995 7025 1178
Size: 30 3976
Fragments arranged by size:
3976 1189 1001 861 618 561 414 222
189 165 131 93 30 30 30 30
30 30 30 30 30 30 30 30
30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2360 2414 2418 2885 3206 3488 3503 5358
Size: 1174 54 4 467 321 282 15 1855
Cuts at: 5358 6149 6158 6161 7003 1186
Size: 791 9 3 842 4006
Fragments arranged by size:
4006 1855 1174 842 791 467 321 282
54 15 9 4 3
BseYI C'CCAG_C
Cuts at: 2266 2600 2958 3914 4267 4426 4542 5148 5281
Size: 334 358 956 353 159 116 606 133
Cuts at: 5281 5925 6098 6373 6378 9026 9522 2266
Size: 644 173 275 5 2648 496 2567
Fragments arranged by size:
2648 2567 956 644 606 496 358 353
334 275 173 159 133 116 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2583 3272 4052 4202 4640 5030 5195
Size: 573 847 689 780 150 438 390 165
Cuts at: 5195 6446 6755 7069 1163
Size: 1251 309 314 3917
Fragments arranged by size:
3917 1251 847 780 689 573 438 390
314 309 165 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6940 7273 8034 9062 1540
Size: 275 186 4939 333 761 1028 2301
Fragments arranged by size:
4939 2301 1028 761 333 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2454 2683 2772 2990 3294 4197 5074 5408
Size: 240 229 89 218 304 903 877 334
Cuts at: 5408 5547 5951 6075 7884 7969 9040 2214
Size: 139 404 124 1809 85 1071 2997
Fragments arranged by size:
2997 1809 1071 903 877 404 334 304
240 229 218 139 124 89 85
BsmI GAATG_Cn'
Cuts at: 2739 4902 7334 7427 2739
Size: 2163 2432 93 5135
Fragments arranged by size:
5135 2432 2163 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4272 5312 5852 6703 6919 4272
Size: 1040 540 851 216 7176
Fragments arranged by size:
7176 1040 851 540 216
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2070 2186 2214 2295 2326 2386
Size: 399 271 9 116 28 81 31 60
Cuts at: 2386 2414 2454 2478 2683 2772 2851 2927 2990
Size: 28 40 24 205 89 79 76 63
Cuts at: 2990 3041 3048 3294 3397 3630 3688 4197 4514
Size: 51 7 246 103 233 58 509 317
Cuts at: 4514 5002 5074 5337 5408 5547 5731 5951 6075
Size: 488 72 263 71 139 184 220 124
Cuts at: 6075 6284 6501 6534 6821 6920 7074 7142 7884
Size: 209 217 33 287 99 154 68 742
Cuts at: 7884 7969 9040 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 488 399 317 287
271 263 246 233 220 217 209 205
184 154 139 124 116 103 99 89
85 81 79 76 72 71 68 63
60 58 51 40 33 31 28 28
24 9 7
BspEI T'CCGG_A
Cuts at: 4048 9486 4048
Size: 5438 4385
BspHI T'CATG_A
Cuts at: 5439 7712 9442 5439
Size: 2273 1730 5820
Fragments arranged by size:
5820 2273 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3244 5153 5325 5473 6266 6678 7178 844
Size: 2400 1909 172 148 793 412 500 3489
Fragments arranged by size:
3489 2400 1909 793 500 412 172 148
BsrBI CCG'CTC
Cuts at: 1394 1823 7710 1394
Size: 429 5887 3507
Fragments arranged by size:
5887 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5768 6183 8318 8492 65
Size: 1183 4520 415 2135 174 1396
Fragments arranged by size:
4520 2135 1396 1183 415 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4458 4634 4726 6939 8467 8654 1700
Size: 110 2648 176 92 2213 1528 187 2869
Fragments arranged by size:
2869 2648 2213 1528 187 176 110 92
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9823
BssSI C'ACGA_G
Cuts at: 4136 6566 7883 8895 4136
Size: 2430 1317 1012 5064
Fragments arranged by size:
5064 2430 1317 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2218 2696 2953 4161 5145 5767 5935 794
Size: 1424 478 257 1208 984 622 168 4682
Fragments arranged by size:
4682 1424 1208 984 622 478 257 168
BstBI TT'CG_AA
Cuts at: 2007 8627 2007
Size: 6620 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9823
BstXI CCAn_nnnn'nTGG
Cuts at: 2600 3637 4297 4402 4886 6838 2600
Size: 1037 660 105 484 1952 5585
Fragments arranged by size:
5585 1952 1037 660 484 105
BstYI r'GATC_y
Cuts at: 1972 2139 2241 2616 3309 3564 3606 3931 4860
Size: 167 102 375 693 255 42 325 929
Cuts at: 4860 4917 5703 7153 7520 7905 7922 9374 9460
Size: 57 786 1450 367 385 17 1452 86
Cuts at: 9460 9472 9818 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1450 929 786 693 385 375
367 346 325 255 167 102 86 57
42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2868 3456 3689 6275 7015 2868
Size: 588 233 2586 740 5676
Fragments arranged by size:
5676 2586 740 588 233
BtgI C'CryG_G
Cuts at: 513 1065 2022 2919 4143 4251 5169 5275 5469
Size: 552 957 897 1224 108 918 106 194
Cuts at: 5469 6754 513
Size: 1285 3582
Fragments arranged by size:
3582 1285 1224 957 918 897 552 194
108 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2181 487
Size: 683 56 955 8129
Fragments arranged by size:
8129 955 683 56
BtsI GCAGTG_nn'
Cuts at: 3149 3375 4118 6117 6368 7086 7339 8133 8153
Size: 226 743 1999 251 718 253 794 20
Cuts at: 8153 3149
Size: 4819
Fragments arranged by size:
4819 1999 794 743 718 253 251 226
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5794 5829 529
Size: 35 5230 35 4523
Fragments arranged by size:
5230 4523 35 35
DraI TTT'AAA
Cuts at: 7127 7127
Size: 9823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2504 6785 8830 809
Size: 507 1188 4281 2045 1802
Fragments arranged by size:
4281 2045 1802 1188 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2188 2278 2916
Size: 54 1425 50 275 18 358 90 638
Cuts at: 2916 3372 3621 4943 5361 6691 7270 8159 9788
Size: 456 249 1322 418 1330 579 889 1629
Cuts at: 9788 8
Size: 43
Fragments arranged by size:
1629 1425 1330 1322 889 638 579 456
418 358 275 249 90 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 7270 1537
Size: 275 5458 4090
Fragments arranged by size:
5458 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2777 3521 4773 4988 6084 6135 6547 6701
Size: 1424 744 1252 215 1096 51 412 154
Cuts at: 6701 7753 1353
Size: 1052 3423
Fragments arranged by size:
3423 1424 1252 1096 1052 744 412 215
154 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 5855 6140 8410 8784 8930
Size: 1064 138 501 3765 285 2270 374 146
Cuts at: 8930 387
Size: 1280
Fragments arranged by size:
3765 2270 1280 1064 501 374 285 146
138
EcoICRI GAG'CTC
Cuts at: 2988 5406 2988
Size: 2418 7405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2417 2505 2578 2918 2924 3058
Size: 393 731 54 88 73 340 6 134
Cuts at: 3058 3326 3433 3545 3554 3753 4112 4181 4340
Size: 268 107 112 9 199 359 69 159
Cuts at: 4340 4498 4847 5029 5261 5422 5660 5894 5909
Size: 158 349 182 232 161 238 234 15
Cuts at: 5909 5975 6041 6479 6821 7886 8483 9270 9538
Size: 66 66 438 342 1065 597 787 268
Cuts at: 9538 1239
Size: 1524
Fragments arranged by size:
1524 1065 787 731 597 438 393 359
349 342 340 268 268 238 234 232
199 182 161 159 158 134 112 107
88 73 69 66 66 54 15 9
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3073 3076 4505 6274 6854 6911 3073
Size: 3 1429 1769 580 57 5985
Fragments arranged by size:
5985 1769 1429 580 57 3
EcoO109I rG'GnC_Cy
Cuts at: 2066 2292 2322 2323 2497 2506 2604 2755 3694
Size: 226 30 1 174 9 98 151 939
Cuts at: 3694 3960 4062 4294 4925 5155 5422 6157 6531
Size: 266 102 232 631 230 267 735 374
Cuts at: 6531 6551 6592 6817 7019 2066
Size: 20 41 225 202 4870
Fragments arranged by size:
4870 939 735 631 374 267 266 232
230 226 225 202 174 151 102 98
41 30 20 9 1
EcoRI G'AATT_C
Cuts at: 2048 7132 7510 2048
Size: 5084 378 4361
Fragments arranged by size:
5084 4361 378
FspI TGC'GCA
Cuts at: 8329 8329
Size: 9823
HaeII r_GCGC'y
Cuts at: 1678 2460 3412 4635 4876 5612 5971 8970 1678
Size: 782 952 1223 241 736 359 2999 2531
Fragments arranged by size:
2999 2531 1223 952 782 736 359 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 2351 2383 2596
Size: 32 135 32 596 32 151 32 213
Cuts at: 2596 2628 2954 2986 4269 4271 4301 4303 4398
Size: 32 326 32 1283 2 30 2 95
Cuts at: 4398 4430 5390 5422 6700 6732 6872 6904 8464
Size: 32 960 32 1278 32 140 32 1560
Cuts at: 8464 8496 8538 8570 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1560 1283 1278 960 596 326 213
151 140 135 95 42 32 32 32
32 32 32 32 32 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2894 3270 7167 7413 669
Size: 988 281 956 376 3897 246 3079
Fragments arranged by size:
3897 3079 988 956 376 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9823
HpaI GTT'AAC
Cuts at: 7413 7413
Size: 9823
KpnI G_GTAC'C
Cuts at: 7148 7148
Size: 9823
MfeI C'AATT_G
Cuts at: 7422 7422
Size: 9823
MluI A'CGCG_T
Cuts at: 9699 9699
Size: 9823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4647 6324 8937 9121 1305
Size: 3285 57 1677 2613 184 2007
Fragments arranged by size:
3285 2613 2007 1677 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2190 2280 2918 3374 4945
Size: 54 1425 343 358 90 638 456 1571
Cuts at: 4945 5363 10
Size: 418 4470
Fragments arranged by size:
4470 1571 1425 638 456 418 358 343
90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 4418 5670 6759 7781 8140 8299
Size: 648 1359 1893 1252 1089 1022 359 159
Cuts at: 8299 518
Size: 2042
Fragments arranged by size:
2042 1893 1359 1252 1089 1022 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2395 2676 2821 3025 3053 3914 3997 4003
Size: 789 281 145 204 28 861 83 6
Cuts at: 4003 4099 4267 5277 5755 5998 7239 7916 9064
Size: 96 168 1010 478 243 1241 677 1148
Cuts at: 9064 9309 1606
Size: 245 2120
Fragments arranged by size:
2120 1241 1148 1010 861 789 677 478
281 245 243 204 168 145 96 83
28 6
NaeI GCC'GGC
Cuts at: 4636 4728 4636
Size: 92 9731
NcoI C'CATG_G
Cuts at: 513 1065 2022 2919 6754 513
Size: 552 957 897 3835 3582
Fragments arranged by size:
3835 3582 957 897 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9823
NgoMIV G'CCGG_C
Cuts at: 4634 4726 4634
Size: 92 9731
NheI G'CTAG_C
Cuts at: 1052 2686 1052
Size: 1634 8189
NotI GC'GGCC_GC
Cuts at: 7270 7270
Size: 9823
NspI r_CATG'y
Cuts at: 1132 1229 2081 2530 2801 3304 4370 4663 4726
Size: 97 852 449 271 503 1066 293 63
Cuts at: 4726 5330 6416 6514 6870 7181 8726 1132
Size: 604 1086 98 356 311 1545 2229
Fragments arranged by size:
2229 1545 1086 1066 852 604 503 449
356 311 293 271 98 97 63
PasI CC'CwG_GG
Cuts at: 1348 2062 3041 5215 6831 1348
Size: 714 979 2174 1616 4340
Fragments arranged by size:
4340 2174 1616 979 714
PciI A'CATG_T
Cuts at: 2526 8722 2526
Size: 6196 3627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 2958 4941 5020 5134 5239 5737 5998
Size: 1002 848 1983 79 114 105 498 261
Cuts at: 5998 6700 1108
Size: 702 4231
Fragments arranged by size:
4231 1983 1002 848 702 498 261 114
105 79
PfoI T'CCnGG_A
Cuts at: 2508 2811 3570 3927 4197 4824 4953 2508
Size: 303 759 357 270 627 129 7378
Fragments arranged by size:
7378 759 627 357 303 270 129
PmeI GTTT'AAAC
Cuts at: 7127 7127
Size: 9823
PmlI CAC'GTG
Cuts at: 2449 5668 2449
Size: 3219 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 5171 5203 5555 5587 6281 6313 6995
Size: 32 2248 32 352 32 694 32 682
Cuts at: 6995 7027 7892 7924 9405 9437 2891
Size: 32 865 32 1481 32 3277
Fragments arranged by size:
3277 2248 1481 865 694 682 352 32
32 32 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2506 2604 2755 3694 3960 4062 6157 6592
Size: 9 98 151 939 266 102 2095 435
Cuts at: 6592 2497
Size: 5728
Fragments arranged by size:
5728 2095 939 435 266 151 102 98
9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9823
PsiI TTA'TAA
Cuts at: 7393 7393
Size: 9823
PspOMI G'GGCC_C
Cuts at: 2066 2291 2322 6530 6817 2066
Size: 225 31 4208 287 5072
Fragments arranged by size:
5072 4208 287 225 31
PspXI vC'TCGA_Gb
Cuts at: 4714 4714
Size: 9823
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 9791
PstI C_TGCA'G
Cuts at: 830 2311 3156 3888 3996 4002 5562 5760 5967
Size: 1481 845 732 108 6 1560 198 207
Cuts at: 5967 6363 6393 7175 830
Size: 396 30 782 3478
Fragments arranged by size:
3478 1560 1481 845 782 732 396 207
198 108 30 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9823
PvuII CAG'CTG
Cuts at: 2395 2821 3025 3914 3997 4099 4267 5755 5998
Size: 426 204 889 83 102 168 1488 243
Cuts at: 5998 2395
Size: 6220
Fragments arranged by size:
6220 1488 889 426 243 204 168 102
83
RsrII CG'GwC_CG
Cuts at: 5842 5842
Size: 9823
SacI G_AGCT'C
Cuts at: 2990 5408 2990
Size: 2418 7405
SacII CC_GC'GG
Cuts at: 5278 5278
Size: 9823
SalI G'TCGA_C
Cuts at: 1936 7165 1936
Size: 5229 4594
SanDI GG'GwC_CC
Cuts at: 2604 3960 2604
Size: 1356 8467
SapI GCTCTTCn'nnn_
Cuts at: 2777 2777
Size: 9823
SbfI CC_TGCA'GG
Cuts at: 7175 7175
Size: 9823
ScaI AGT'ACT
Cuts at: 8071 9783 8071
Size: 1712 8111
SfcI C'TryA_G
Cuts at: 826 1045 2041 2307 3152 3884 3992 3998 5558
Size: 219 996 266 845 732 108 6 1560
Cuts at: 5558 5756 5963 6359 6389 7049 7171 8306 8987
Size: 198 207 396 30 660 122 1135 681
Cuts at: 8987 9178 826
Size: 191 1471
Fragments arranged by size:
1560 1471 1135 996 845 732 681 660
396 266 219 207 198 191 122 108
30 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9823
SmaI CCC'GGG
Cuts at: 1872 5420 7064 7150 1872
Size: 3548 1644 86 4545
Fragments arranged by size:
4545 3548 1644 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4714 5566 5863 6335 6596 6746
Size: 197 926 2771 852 297 472 261 150
Cuts at: 6746 7927 8828 9090 820
Size: 1181 901 262 1553
Fragments arranged by size:
2771 1553 1181 926 901 852 472 297
262 261 197 150
SnaBI TAC'GTA
Cuts at: 493 7120 7614 493
Size: 6627 494 2702
Fragments arranged by size:
6627 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9823
SphI G_CATG'C
Cuts at: 1132 4370 6870 7181 1132
Size: 3238 2500 311 3774
Fragments arranged by size:
3774 3238 2500 311
SspI AAT'ATT
Cuts at: 5 52 5554 7747 5
Size: 47 5502 2193 2081
Fragments arranged by size:
5502 2193 2081 47
StuI AGG'CCT
Cuts at: 1517 2969 5006 1517
Size: 1452 2037 6334
Fragments arranged by size:
6334 2037 1452
StyI C'CwwG_G
Cuts at: 513 1065 2022 2232 2501 2919 2981 3361 4290
Size: 552 957 210 269 418 62 380 929
Cuts at: 4290 4921 5409 6052 6754 6850 7262 513
Size: 631 488 643 702 96 412 3074
Fragments arranged by size:
3074 957 929 702 643 631 552 488
418 412 380 269 210 96 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3277 5888 7109 7850 7867 8020 8205
Size: 1255 1697 2611 1221 741 17 153 185
Cuts at: 8205 8650 325
Size: 445 1498
Fragments arranged by size:
2611 1697 1498 1255 1221 741 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8069 9781 96
Size: 274 80 33 51 1443 6092 1712 138
Fragments arranged by size:
6092 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 2848 2880 4110 4142 4523 4555 6980
Size: 32 412 32 1230 32 381 32 2425
Cuts at: 6980 7012 9783 9815 2404
Size: 32 2771 32 2412
Fragments arranged by size:
2771 2425 2412 1230 412 381 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9823
XbaI T'CTAG_A
Cuts at: 3769 3934 5057 7159 3769
Size: 165 1123 2102 6433
Fragments arranged by size:
6433 2102 1123 165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3382 3382
Size: 9823
XhoI C'TCGA_G
Cuts at: 1943 4714 5863 1943
Size: 2771 1149 5903
Fragments arranged by size:
5903 2771 1149
XmaI C'CCGG_G
Cuts at: 1870 5418 7062 7148 1870
Size: 3548 1644 86 4545
Fragments arranged by size:
4545 3548 1644 86
XmnI GAAnn'nnTTC
Cuts at: 7952 7952
Size: 9823
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6883 7631 276
Size: 53 83 186 936 5349 748 2468
Fragments arranged by size:
5349 2468 936 748 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI SexAI
SfiI SfoI SrfI SwaI