Restriction map for
pFN21ASDA0070
(Circular) (Six-base) MAPSORT of: pFN21ASDA0070.seq Check: 5832 from: 1 to: 6520
pFN21ASDA0070 6520 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2373 3575 2373
Size: 1202 5318
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2325 3518 3813 3863 1937
Size: 388 1193 295 50 4594
Fragments arranged by size:
4594 1193 388 295 50
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2303 3291 4583 5967 6235 1632
Size: 479 192 988 1292 1384 268 1917
Fragments arranged by size:
1917 1384 1292 988 479 268 192
AfeI AGC'GCT
Cuts at: 3476 3476
Size: 6520
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 5419 6396 1203
Size: 4216 977 1327
Fragments arranged by size:
4216 1327 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 4263 5835 4263
Size: 1572 4948
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2525 2750 3285 3829 4055 4207 6316 1493
Size: 1032 225 535 544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 544 535 226 225 152
AseI AT'TA_AT
Cuts at: 160 1032 2538 5074 160
Size: 872 1506 2536 1606
Fragments arranged by size:
2536 1606 1506 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 3845 1870
Size: 73 1902 4545
Fragments arranged by size:
4545 1902 73
BamHI G'GATC_C
Cuts at: 2415 3850 4217 2415
Size: 1435 367 4718
Fragments arranged by size:
4718 1435 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3447 3841 5296 618
Size: 325 217 627 1660 394 1455 1842
Fragments arranged by size:
1842 1660 1455 627 394 325 217
BanII G_rGCy'C
Cuts at: 1391 2151 3434 3839 1391
Size: 760 1283 405 4072
Fragments arranged by size:
4072 1283 760 405
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 3756 928
Size: 971 405 1452 3692
Fragments arranged by size:
3692 1452 971 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 3412 1548
Size: 1864 4656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 3159 5131
Size: 107 122 71 631 605 364 1123 1972
Cuts at: 5131 136
Size: 1525
Fragments arranged by size:
1972 1525 1123 631 605 364 122 107
71
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 2165 2609 3937 2165
Size: 444 1328 4748
Fragments arranged by size:
4748 1328 444
Bme1580I G_kGCm'C
Cuts at: 1790 4581 5737 1790
Size: 2791 1156 2573
Fragments arranged by size:
2791 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4251 5209 442
Size: 416 53 83 399 24 2834 958 1753
Fragments arranged by size:
2834 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2934 2934
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2904 5180 1239
Size: 1665 2276 2579
Fragments arranged by size:
2579 2276 1665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3242 4645 5510 5808 3242
Size: 1403 865 298 3954
Fragments arranged by size:
3954 1403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5183 882
Size: 617 3684 2219
Fragments arranged by size:
3684 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3817 4311 493
Size: 711 2613 494 2702
Fragments arranged by size:
2702 2613 711 494
BsaBI GATnn'nnATC
Cuts at: 2837 6514 2837
Size: 3677 2843
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2098 2980 4327 4709
Size: 53 83 186 936 565 882 1347 382
Cuts at: 4709 5344 275
Size: 635 1451
Fragments arranged by size:
1451 1347 936 882 635 565 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2988 4953 5351 5625 5772 6183 2988
Size: 1965 398 274 147 411 3325
Fragments arranged by size:
3325 1965 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2460 2490 1178
Size: 30 165 30 1057 30 5208
Fragments arranged by size:
5208 1057 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2481 1186
Size: 1295 5225
BseYI C'CCAG_C
Cuts at: 2803 3660 5723 6219 2803
Size: 857 2063 496 3104
Fragments arranged by size:
3104 2063 857 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 1163
Size: 573 318 5629
Fragments arranged by size:
5629 573 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2043 2962 3970 4731 5759
Size: 275 186 30 12 919 1008 761 1028
Cuts at: 5759 1540
Size: 2301
Fragments arranged by size:
2301 1028 1008 919 761 275 186 30
12
BsiHKAI G_wGCw'C
Cuts at: 2151 4581 4666 5737 2151
Size: 2430 85 1071 2934
Fragments arranged by size:
2934 2430 1071 85
BsmI GAATG_Cn'
Cuts at: 3326 4031 4124 3326
Size: 705 93 5722
Fragments arranged by size:
5722 705 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2089 2089
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 3434 3839 4581 4666 5737 1391
Size: 399 361 1283 405 742 85 1071 2174
Fragments arranged by size:
2174 1283 1071 742 405 399 361 85
BspEI T'CCGG_A
Cuts at: 2988 6183 2988
Size: 3195 3325
BspHI T'CATG_A
Cuts at: 3492 4409 6139 3492
Size: 917 1730 3873
Fragments arranged by size:
3873 1730 917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2373 2399 3575 3875 844
Size: 1529 26 1176 300 3489
Fragments arranged by size:
3489 1529 1176 300 26
BsrBI CCG'CTC
Cuts at: 1394 1823 2763 3453 4407 1394
Size: 429 940 690 954 3507
Fragments arranged by size:
3507 954 940 690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3517 5015 5189 65
Size: 1183 2269 1498 174 1396
Fragments arranged by size:
2269 1498 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5164 5351 1700
Size: 110 3354 187 2869
Fragments arranged by size:
3354 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 2007 5324 2007
Size: 3317 3203
BstEII G'GTnAC_C
Cuts at: 1184 3133 1184
Size: 1949 4571
BstXI CCAn_nnnn'nTGG
Cuts at: 3149 3149
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 2415 3850 4217 4602 4619 6071 6157 6169
Size: 443 1435 367 385 17 1452 86 12
Cuts at: 6169 6515 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1435 443 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3519 3519
Size: 6520
Bsu36I CC'TnA_GG
Cuts at: 2220 2220
Size: 6520
BtgI C'CryG_G
Cuts at: 513 1065 2022 2856 513
Size: 552 957 834 4177
Fragments arranged by size:
4177 957 834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 2633 4036 4830 4850 2175
Size: 458 1403 794 20 3845
Fragments arranged by size:
3845 1403 794 458 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2040 2493
Size: 54 1425 50 275 18 198 12 453
Cuts at: 2493 2859 3967 4856 6485 8
Size: 366 1108 889 1629 43
Fragments arranged by size:
1629 1425 1108 889 453 366 275 198
54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2028 2040 3967 1537
Size: 275 216 12 1927 4090
Fragments arranged by size:
4090 1927 275 216 12
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2220 3210 4450 1353
Size: 734 133 990 1240 3423
Fragments arranged by size:
3423 1240 990 734 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2149 2149
Size: 6520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2303 2904 3291 4583 5180 5967
Size: 393 479 192 601 387 1292 597 787
Cuts at: 5967 6235 1239
Size: 268 1524
Fragments arranged by size:
1524 1292 787 601 597 479 393 387
268 192
EcoO109I rG'GnC_Cy
Cuts at: 2216 2216
Size: 6520
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
EcoRV GAT'ATC
Cuts at: 3298 3298
Size: 6520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 6520
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 3478 5667 1678
Size: 1800 2189 2531
Fragments arranged by size:
2531 2189 1800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2809 2841 3001 3033 5161
Size: 32 135 32 1237 32 160 32 2128
Cuts at: 5161 5193 5235 5267 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2128 1237 160 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 3864 4110 669
Size: 988 281 1240 686 246 3079
Fragments arranged by size:
3079 1240 988 686 281 246
HindIII A'AGCT_T
Cuts at: 748 3359 748
Size: 2611 3909
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3729 5634 5818 1305
Size: 2424 1905 184 2007
Fragments arranged by size:
2424 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 2861 10
Size: 54 1425 343 663 366 3669
Fragments arranged by size:
3669 1425 663 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3147 4478 4837 4996 518
Size: 648 1981 1331 359 159 2042
Fragments arranged by size:
2042 1981 1331 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2281 3547 3656 3936 4613 5761 6006 1606
Size: 675 1266 109 280 677 1148 245 2120
Fragments arranged by size:
2120 1266 1148 677 675 280 245 109
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5011
Fragments arranged by size:
5011 957 552
NdeI CA'TA_TG
Cuts at: 387 2655 387
Size: 2268 4252
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 3878 5423 1132
Size: 97 2649 1545 2229
Fragments arranged by size:
2649 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2846 3232 1348
Size: 1498 386 4636
Fragments arranged by size:
4636 1498 386
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6520
PfoI T'CCnGG_A
Cuts at: 3375 3375
Size: 6520
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 4589 4621 6102 6134 3587
Size: 32 970 32 1481 32 3973
Fragments arranged by size:
3973 1481 970 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2216 2216
Size: 6520
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 2511 4090 2511
Size: 1579 4941
PstI C_TGCA'G
Cuts at: 830 2370 3586 3872 830
Size: 1540 1216 286 3478
Fragments arranged by size:
3478 1540 1216 286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2281 3656 2281
Size: 1375 5145
SacI G_AGCT'C
Cuts at: 2151 2151
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SapI GCTCTTCn'nnn_
Cuts at: 3210 3210
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 3723 4768 6480 3723
Size: 1045 1712 3763
Fragments arranged by size:
3763 1712 1045
SfcI C'TryA_G
Cuts at: 826 1045 2366 3582 3868 5003 5684 5875 826
Size: 219 1321 1216 286 1135 681 191 1471
Fragments arranged by size:
1471 1321 1216 1135 681 286 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3159 3159
Size: 6520
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 3847 1872
Size: 1975 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3221 4624 5525 5787 820
Size: 197 926 1278 1403 901 262 1553
Fragments arranged by size:
1553 1403 1278 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3817 4311 493
Size: 3324 494 2702
Fragments arranged by size:
3324 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6520
StyI C'CwwG_G
Cuts at: 513 1065 2022 3570 3576 3959 513
Size: 552 957 1548 6 383 3074
Fragments arranged by size:
3074 1548 957 552 383 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3159 3432 4547 4564 4717 4902 5347
Size: 1255 1579 273 1115 17 153 185 445
Cuts at: 5347 325
Size: 1498
Fragments arranged by size:
1579 1498 1255 1115 445 273 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 3721 4766
Size: 274 80 33 51 1443 1646 98 1045
Cuts at: 4766 6478 96
Size: 1712 138
Fragments arranged by size:
1712 1646 1443 1045 274 138 98 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2301 2333 2460 2492 6480 6512 2301
Size: 32 127 32 3988 32 2309
Fragments arranged by size:
3988 2309 127 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3781 3781
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 3845 1870
Size: 1975 4545
XmnI GAAnn'nnTTC
Cuts at: 2529 3723 4649 2529
Size: 1194 926 4400
Fragments arranged by size:
4400 1194 926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI