Restriction map for
pFN21ASDA0069
(Circular) (Six-base) MAPSORT of: pFN21ASDA0069.seq Check: 8551 from: 1 to: 5338
pFN21ASDA0069 5338 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2167
Size: 5338
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 2073 2483 4067 2073
Size: 410 1584 3344
Fragments arranged by size:
3344 1584 410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 4453
AlwNI CAG_nnn'CTG
Cuts at: 2069 3081 4653 2069
Size: 1012 1572 2754
Fragments arranged by size:
2754 1572 1012
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2292 2343 2647 2873 3025 5134 1493
Size: 799 51 304 226 152 2109 1697
Fragments arranged by size:
2109 1697 799 304 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 1870
Size: 73 720 4545
Fragments arranged by size:
4545 720 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2659 4114 618
Size: 325 217 627 872 1455 1842
Fragments arranged by size:
1842 1455 872 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 3399 4555 1790
Size: 1609 1156 2573
Fragments arranged by size:
2573 1609 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3998 1239
Size: 2759 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3129 493
Size: 711 1431 494 2702
Fragments arranged by size:
2702 1431 711 494
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3145 3527 4162 275
Size: 53 83 186 936 1612 382 635 1451
Fragments arranged by size:
1612 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 4541 5037 4541
Size: 496 4842
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3399 3484 4555 3399
Size: 85 1071 4182
Fragments arranged by size:
4182 1071 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2657 3399 3484 4555 1391
Size: 399 867 742 85 1071 2174
Fragments arranged by size:
2174 1071 867 742 399 85
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 2436 3227 4957 2436
Size: 791 1730 2817
Fragments arranged by size:
2817 1730 791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2167 2502 2693 844
Size: 1217 106 335 191 3489
Fragments arranged by size:
3489 1217 335 191 106
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3982 4169 1700
Size: 110 2172 187 2869
Fragments arranged by size:
2869 2172 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2069 794
Size: 1275 4063
BstBI TT'CG_AA
Cuts at: 2007 4142 2007
Size: 2135 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2209 2668 3035 3420 3437 4889 4975 4987
Size: 237 459 367 385 17 1452 86 12
Cuts at: 4987 5333 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 459 385 367 346 237 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 3829
Fragments arranged by size:
3829 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 2785 3674
Size: 54 1425 50 275 18 750 205 889
Cuts at: 3674 5303 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 750 275 205 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 3268 1353
Size: 1052 863 3423
Fragments arranged by size:
3423 1052 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3925 4299 4445 387
Size: 1064 138 453 1883 374 146 1280
Fragments arranged by size:
1883 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3401 3998 4785 5053 1239
Size: 393 1769 597 787 268 1524
Fragments arranged by size:
1769 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 5338
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2288 4485 1678
Size: 610 2197 2531
Fragments arranged by size:
2531 2197 610
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2469 2501 3979
Size: 32 135 32 487 32 378 32 1478
Cuts at: 3979 4011 4053 4085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1478 487 378 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 2766
Fragments arranged by size:
2766 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2441 2576 3296 3655 3814 518
Size: 648 1005 270 135 720 359 159 2042
Fragments arranged by size:
2042 1005 720 648 359 270 159 135
MspA1I CmG'CkG
Cuts at: 1606 2089 2754 3431 4579 4824 1606
Size: 483 665 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 665 483 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 3829
Fragments arranged by size:
3829 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2310 1108
Size: 1202 4136
PfoI T'CCnGG_A
Cuts at: 2536 2536
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2065 2690 830
Size: 1235 625 3478
Fragments arranged by size:
3478 1235 625
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2089 2089
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2061 2686 3821 4502 4693 826
Size: 219 1016 625 1135 681 191 1471
Fragments arranged by size:
1471 1135 1016 681 625 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2635 3129 493
Size: 2142 494 2702
Fragments arranged by size:
2702 2142 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2022 2777 513
Size: 552 957 755 3074
Fragments arranged by size:
3074 957 755 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2461 3365 3382 3535 3720 4165
Size: 1255 579 302 904 17 153 185 445
Cuts at: 4165 325
Size: 1498
Fragments arranged by size:
1498 1255 904 579 445 302 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 2568 3584
Size: 274 80 33 51 1443 453 138 1016
Cuts at: 3584 5296 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1016 453 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2206 2674 2206
Size: 468 4870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2170
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI