Restriction map for
pFN21ASDA0067
(Circular) (Six-base) MAPSORT of: pFN21ASDA0067.seq Check: 5774 from: 1 to: 8602
pFN21ASDA0067 8602 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3400 3418 3425 3400
Size: 18 7 8577
Fragments arranged by size:
8577 18 7
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5890 6412 278
Size: 53 83 186 936 4354 522 2468
Fragments arranged by size:
4354 2468 936 522 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2743 4342 4671 5945 1937
Size: 806 1599 329 1274 4594
Fragments arranged by size:
4594 1599 1274 806 329
Acc65I G'GTAC_C
Cuts at: 5923 5923
Size: 8602
AclI AA'CG_TT
Cuts at: 1563 6729 7102 1563
Size: 5166 373 3063
Fragments arranged by size:
5166 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 4737 4753 5253 5463 6665 8049 8317
Size: 468 2637 16 500 210 1202 1384 268
Cuts at: 8317 1632
Size: 1917
Fragments arranged by size:
2637 1917 1384 1202 500 468 268 210
16
AflII C'TTAA_G
Cuts at: 820 1017 2491 820
Size: 197 1474 6931
Fragments arranged by size:
6931 1474 197
AflIII A'CryG_T
Cuts at: 1203 3275 4392 7501 8478 1203
Size: 2072 1117 3109 977 1327
Fragments arranged by size:
3109 2072 1327 1117 977
AgeI A'CCGG_T
Cuts at: 3338 4404 7433 3338
Size: 1066 3029 4507
Fragments arranged by size:
4507 3029 1066
AhdI GACnn_n'nnGTC
Cuts at: 7331 7331
Size: 8602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 4413 4445 3200
Size: 32 1181 32 7357
Fragments arranged by size:
7357 1181 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2560 3242 3334 3515 3626 4190 6345 7917 2560
Size: 682 92 181 111 564 2155 1572 3245
Fragments arranged by size:
3245 2155 1572 682 564 181 111 92
ApaI G_GGCC'C
Cuts at: 3697 3697
Size: 8602
ApaLI G'TGCA_C
Cuts at: 4396 6659 7815 4396
Size: 2263 1156 5183
Fragments arranged by size:
5183 2263 1156
ApoI r'AATT_y
Cuts at: 1493 2631 2720 4193 5911 6137 6289 8398 1493
Size: 1138 89 1473 1718 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 1473 1138 226 152 89
AseI AT'TA_AT
Cuts at: 160 1032 7156 160
Size: 872 6124 1606
Fragments arranged by size:
6124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8602
AvaI C'yCGr_G
Cuts at: 1870 1943 3117 3774 4159 4815 5514 5927 1870
Size: 73 1174 657 385 656 699 413 4545
Fragments arranged by size:
4545 1174 699 657 656 413 385 73
BamHI G'GATC_C
Cuts at: 3150 4003 5932 6299 3150
Size: 853 1929 367 5453
Fragments arranged by size:
5453 1929 853 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2669 5923 7378 618
Size: 325 217 627 882 3254 1455 1842
Fragments arranged by size:
3254 1842 1455 882 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3213 3524 3697 3708 5921 1391
Size: 1822 311 173 11 2213 4072
Fragments arranged by size:
4072 2213 1822 311 173 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 3003 3664 5287 5719 5882 928
Size: 971 120 984 661 1623 432 163 3648
Fragments arranged by size:
3648 1623 984 971 661 432 163 120
BbvCI CC'TCA_GC
Cuts at: 3235 3640 3235
Size: 405 8197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6793 6827 1903
Size: 34 4856 34 3678
Fragments arranged by size:
4856 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2964 4166 4481 5728 6494 7704 2964
Size: 1202 315 1247 766 1210 3862
Fragments arranged by size:
3862 1247 1210 1202 766 315
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8602
BfrBI ATG'CAT
Cuts at: 2276 5269 2276
Size: 2993 5609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7213 136
Size: 107 122 71 631 605 5541 1525
Fragments arranged by size:
5541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4873 8597 4873
Size: 3724 4878
BlpI GC'TnA_GC
Cuts at: 6019 6019
Size: 8602
Bme1580I G_kGCm'C
Cuts at: 1790 3697 4400 6663 7819 1790
Size: 1907 703 2263 1156 2573
Fragments arranged by size:
2573 2263 1907 1156 703
BmgBI CAC'GTC
Cuts at: 1318 3278 3498 1318
Size: 1960 220 6422
Fragments arranged by size:
6422 1960 220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 3346 3460
Size: 416 53 83 399 24 712 1217 114
Cuts at: 3460 3662 6333 7291 442
Size: 202 2671 958 1753
Fragments arranged by size:
2671 1753 1217 958 712 416 399 202
114 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2207 1056
Size: 1151 7451
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2469 5110 2469
Size: 2641 5961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2610 3227 3269 3642 3707 4007 7262
Size: 947 424 617 42 373 65 300 3255
Cuts at: 7262 1239
Size: 2579
Fragments arranged by size:
3255 2579 947 617 424 373 300 65
42
Bpu10I CC'TnA_GC
Cuts at: 2894 3235 3269 3640 2894
Size: 341 34 371 7856
Fragments arranged by size:
7856 371 341 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2490 2596 3050 6727 7592 7890 2490
Size: 106 454 3677 865 298 3202
Fragments arranged by size:
3677 3202 865 454 298 106
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3923 7265 882
Size: 617 2424 3342 2219
Fragments arranged by size:
3342 2424 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3105 5899 6393 493
Size: 711 1901 2794 494 2702
Fragments arranged by size:
2794 2702 1901 711 494
BsaBI GATnn'nnATC
Cuts at: 2835 8596 2835
Size: 5761 2841
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 5887 6409 6791
Size: 53 83 186 936 2332 2022 522 382
Cuts at: 6791 7426 275
Size: 635 1451
Fragments arranged by size:
2332 2022 1451 936 635 522 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3338 4404 7035 7433 7707 7854 8265 3338
Size: 1066 2631 398 274 147 411 3675
Fragments arranged by size:
3675 2631 1066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 3200 3230 4108
Size: 30 165 30 903 30 864 30 878
Cuts at: 4108 4138 1178
Size: 30 5642
Fragments arranged by size:
5642 903 878 864 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3038 3140 3313 5648 1186
Size: 1852 102 173 2335 4140
Fragments arranged by size:
4140 2335 1852 173 102
BseYI C'CCAG_C
Cuts at: 2329 2441 3631 3697 4464 5063 5792 7805 8301
Size: 112 1190 66 767 599 729 2013 496
Cuts at: 8301 2329
Size: 2630
Fragments arranged by size:
2630 2013 1190 767 729 599 496 112
66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3228 3534 1163
Size: 573 1492 306 6231
Fragments arranged by size:
6231 1492 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5164 6052 6813 7841 1540
Size: 275 186 3163 888 761 1028 2301
Fragments arranged by size:
3163 2301 1028 888 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3497 3721 4400 6663 6748 7819 3497
Size: 224 679 2263 85 1071 4280
Fragments arranged by size:
4280 2263 1071 679 224 85
BsmI GAATG_Cn'
Cuts at: 5882 6113 6206 5882
Size: 231 93 8278
Fragments arranged by size:
8278 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3213 3497 3524 3697 3708 3721 4400
Size: 399 1423 284 27 173 11 13 679
Cuts at: 4400 5921 6663 6748 7819 1391
Size: 1521 742 85 1071 2174
Fragments arranged by size:
2174 1521 1423 1071 742 679 399 284
173 85 27 13 11
BspEI T'CCGG_A
Cuts at: 8265 8265
Size: 8602
BspHI T'CATG_A
Cuts at: 6491 8221 6491
Size: 1730 6872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3400 3418 3425 3469 5957 844
Size: 2556 18 7 44 2488 3489
Fragments arranged by size:
3489 2556 2488 44 18 7
BsrBI CCG'CTC
Cuts at: 1394 1823 3691 6489 1394
Size: 429 1868 2798 3507
Fragments arranged by size:
3507 2798 1868 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4488 7097 7271 65
Size: 1183 3240 2609 174 1396
Fragments arranged by size:
3240 2609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2964 3338 4404 7246 7433 1700
Size: 110 1154 374 1066 2842 187 2869
Fragments arranged by size:
2869 2842 1154 1066 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 2182 96
Size: 2086 6516
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8602
BssSI C'ACGA_G
Cuts at: 6662 7674 6662
Size: 1012 7590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8602
BstBI TT'CG_AA
Cuts at: 2007 7406 2007
Size: 5399 3203
BstEII G'GTnAC_C
Cuts at: 1184 2609 1184
Size: 1425 7177
BstXI CCAn_nnnn'nTGG
Cuts at: 2693 4691 2693
Size: 1998 6604
BstYI r'GATC_y
Cuts at: 1972 2557 3150 3183 3331 3512 4003 4873 5932
Size: 585 593 33 148 181 491 870 1059
Cuts at: 5932 6299 6684 6701 8153 8239 8251 8597 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1059 870 593 585 491 385
367 346 181 148 86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 5101 5101
Size: 8602
BtgI C'CryG_G
Cuts at: 513 1065 3054 3676 3723 5539 513
Size: 552 1989 622 47 1816 3576
Fragments arranged by size:
3576 1989 1816 622 552 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7863
Fragments arranged by size:
7863 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 2292 3033 4669 5376 5839 6118 6912 6932
Size: 180 741 1636 707 463 279 794 20
Cuts at: 6932 2112
Size: 3782
Fragments arranged by size:
3782 1636 794 741 707 463 279 180
20
ClaI AT'CG_AT
Cuts at: 1554 2154 1554
Size: 600 8002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8567
DraI TTT'AAA
Cuts at: 5906 5906
Size: 8602
DraIII CAC_nnn'GTG
Cuts at: 3108 3557 3914 3108
Size: 449 357 7796
Fragments arranged by size:
7796 449 357
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2618 7609 809
Size: 507 1302 4991 1802
Fragments arranged by size:
4991 1802 1302 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2966 3073 6049
Size: 54 1425 50 275 18 1136 107 2976
Cuts at: 6049 6938 8567 8
Size: 889 1629 43
Fragments arranged by size:
2976 1629 1425 1136 889 275 107 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6049 1537
Size: 275 4237 4090
Fragments arranged by size:
4237 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2537 2639 3161 3986 4329 4713 4938
Size: 723 461 102 522 825 343 384 225
Cuts at: 4938 6532 1353
Size: 1594 3423
Fragments arranged by size:
3423 1594 825 723 522 461 384 343
225 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3865 4804 7189 7563 7709 387
Size: 1064 138 2276 939 2385 374 146 1280
Fragments arranged by size:
2385 2276 1280 1064 939 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2186 2610 3227 3269 3642 3707
Size: 393 468 86 424 617 42 373 65
Cuts at: 3707 4007 4737 4753 5253 5463 6665 7262 8049
Size: 300 730 16 500 210 1202 597 787
Cuts at: 8049 8317 1239
Size: 268 1524
Fragments arranged by size:
1524 1202 787 730 617 597 500 468
424 393 373 300 268 210 86 65
42 16
EcoO109I rG'GnC_Cy
Cuts at: 3308 3694 4187 4711 3308
Size: 386 493 524 7199
Fragments arranged by size:
7199 524 493 386
EcoRI G'AATT_C
Cuts at: 2631 5911 6289 2631
Size: 3280 378 4944
Fragments arranged by size:
4944 3280 378
EcoRV GAT'ATC
Cuts at: 5419 5419
Size: 8602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2690 3904 2690
Size: 1214 7388
FspI TGC'GCA
Cuts at: 7108 7108
Size: 8602
HaeII r_GCGC'y
Cuts at: 1678 7749 1678
Size: 6071 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2306 2338 2339
Size: 32 135 32 689 32 13 32 1
Cuts at: 2339 2351 2371 2383 4281 4313 4721 4753 5097
Size: 12 20 12 1898 32 408 32 344
Cuts at: 5097 5129 7243 7275 7317 7349 1373
Size: 32 2114 32 42 32 2626
Fragments arranged by size:
2626 2114 1898 689 408 344 135 42
32 32 32 32 32 32 32 32
32 20 13 12 12 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2257 2536 3874 5407 5946 6192
Size: 988 281 319 279 1338 1533 539 246
Cuts at: 6192 669
Size: 3079
Fragments arranged by size:
3079 1533 1338 988 539 319 281 279
246
HindIII A'AGCT_T
Cuts at: 748 3731 5660 748
Size: 2983 1929 3690
Fragments arranged by size:
3690 2983 1929
HpaI GTT'AAC
Cuts at: 3874 6192 3874
Size: 2318 6284
KpnI G_GTAC'C
Cuts at: 5927 5927
Size: 8602
MfeI C'AATT_G
Cuts at: 6201 6201
Size: 8602
MluI A'CGCG_T
Cuts at: 8478 8478
Size: 8602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2925 5328 7716 7900 1305
Size: 1620 2403 2388 184 2007
Fragments arranged by size:
2403 2388 2007 1620 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3075 10
Size: 54 1425 343 1243 5537
Fragments arranged by size:
5537 1425 1243 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6560 6919 7078 518
Size: 648 5394 359 159 2042
Fragments arranged by size:
5394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4476 4701 5402 6018 6695 7843 8088 1606
Size: 2870 225 701 616 677 1148 245 2120
Fragments arranged by size:
2870 2120 1148 701 677 616 245 225
NaeI GCC'GGC
Cuts at: 2966 2966
Size: 8602
NcoI C'CATG_G
Cuts at: 513 1065 3054 3676 3723 5539 513
Size: 552 1989 622 47 1816 3576
Fragments arranged by size:
3576 1989 1816 622 552 47
NdeI CA'TA_TG
Cuts at: 387 4009 387
Size: 3622 4980
NgoMIV G'CCGG_C
Cuts at: 2964 2964
Size: 8602
NheI G'CTAG_C
Cuts at: 1052 2203 1052
Size: 1151 7451
NotI GC'GGCC_GC
Cuts at: 6049 6049
Size: 8602
NsiI A_TGCA'T
Cuts at: 2278 5271 2278
Size: 2993 5609
NspI r_CATG'y
Cuts at: 1132 1229 2629 3685 4396 5960 7505 1132
Size: 97 1400 1056 711 1564 1545 2229
Fragments arranged by size:
2229 1564 1545 1400 1056 711 97
PasI CC'CwG_GG
Cuts at: 1348 5757 1348
Size: 4409 4193
PciI A'CATG_T
Cuts at: 4392 7501 4392
Size: 3109 5493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4877 1108
Size: 3769 4833
PfoI T'CCnGG_A
Cuts at: 3159 3984 3159
Size: 825 7777
PmeI GTTT'AAAC
Cuts at: 5906 5906
Size: 8602
PmlI CAC'GTG
Cuts at: 3105 3105
Size: 8602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4823 4855 6671 6703 8184 8216 3200
Size: 32 1591 32 1816 32 1481 32 3586
Fragments arranged by size:
3586 1816 1591 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4711 4711
Size: 8602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8602
PsiI TTA'TAA
Cuts at: 6172 6172
Size: 8602
PspOMI G'GGCC_C
Cuts at: 3693 3693
Size: 8602
PstI C_TGCA'G
Cuts at: 830 3758 5954 830
Size: 2928 2196 3478
Fragments arranged by size:
3478 2928 2196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8602
SalI G'TCGA_C
Cuts at: 1936 5944 1936
Size: 4008 4594
SapI GCTCTTCn'nnn_
Cuts at: 2537 2537
Size: 8602
SbfI CC_TGCA'GG
Cuts at: 5954 5954
Size: 8602
ScaI AGT'ACT
Cuts at: 4580 6850 8562 4580
Size: 2270 1712 4620
Fragments arranged by size:
4620 2270 1712
SfcI C'TryA_G
Cuts at: 826 1045 2067 2379 3381 3754 4141 4272 4436
Size: 219 1022 312 1002 373 387 131 164
Cuts at: 4436 4914 5397 5950 7085 7766 7957 826
Size: 478 483 553 1135 681 191 1471
Fragments arranged by size:
1471 1135 1022 1002 681 553 483 478
387 373 312 219 191 164 131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8602
SmaI CCC'GGG
Cuts at: 1872 4161 4817 5929 1872
Size: 2289 656 1112 4545
Fragments arranged by size:
4545 2289 1112 656
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2491 2505 2575 3065 3774 6706
Size: 197 926 548 14 70 490 709 2932
Cuts at: 6706 7607 7869 820
Size: 901 262 1553
Fragments arranged by size:
2932 1553 926 901 709 548 490 262
197 70 14
SnaBI TAC'GTA
Cuts at: 493 5899 6393 493
Size: 5406 494 2702
Fragments arranged by size:
5406 2702 494
SpeI A'CTAG_T
Cuts at: 152 5261 152
Size: 5109 3493
SphI G_CATG'C
Cuts at: 1132 2629 3685 5960 1132
Size: 1497 1056 2275 3774
Fragments arranged by size:
3774 2275 1497 1056
SspI AAT'ATT
Cuts at: 5 52 6526 5
Size: 47 6474 2081
Fragments arranged by size:
6474 2081 47
StuI AGG'CCT
Cuts at: 1517 5093 1517
Size: 3576 5026
StyI C'CwwG_G
Cuts at: 513 1065 3054 3676 3723 4854 5539 6041 513
Size: 552 1989 622 47 1131 685 502 3074
Fragments arranged by size:
3074 1989 1131 685 622 552 502 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2028 3278 4038 4212 4559 4966 5178
Size: 1255 448 1250 760 174 347 407 212
Cuts at: 5178 5749 6629 6646 6799 6984 7429 325
Size: 571 880 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1250 880 760 571 448 445
407 347 212 185 174 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2182 3321 4320
Size: 274 80 33 51 1443 205 1139 999
Cuts at: 4320 4578 6848 8560 96
Size: 258 2270 1712 138
Fragments arranged by size:
2270 1712 1443 1139 999 274 258 205
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3089 3121 4108 4140 8562 8594 3089
Size: 32 987 32 4422 32 3097
Fragments arranged by size:
4422 3097 987 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5326 1316
Size: 4010 4592
XbaI T'CTAG_A
Cuts at: 5938 5938
Size: 8602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3052 4960 5624 3052
Size: 1908 664 6030
Fragments arranged by size:
6030 1908 664
XhoI C'TCGA_G
Cuts at: 1943 3774 1943
Size: 1831 6771
XmaI C'CCGG_G
Cuts at: 1870 4159 4815 5927 1870
Size: 2289 656 1112 4545
Fragments arranged by size:
4545 2289 1112 656
XmnI GAAnn'nnTTC
Cuts at: 2315 3734 4086 5238 5726 6731 2315
Size: 1419 352 1152 488 1005 4186
Fragments arranged by size:
4186 1419 1152 1005 488 352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5888 6410 276
Size: 53 83 186 936 4354 522 2468
Fragments arranged by size:
4354 2468 936 522 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
EcoICRI EcoNI FseI FspAI KasI NarI NruI PacI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI