Restriction map for
pFN21ASDA0065
(Circular) (Six-base) MAPSORT of: pFN21ASDA0065.seq Check: 5508 from: 1 to: 7159
pFN21ASDA0065 7159 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4969 278
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4502 1937
Size: 2565 4594
Acc65I G'GTAC_C
Cuts at: 4480 4480
Size: 7159
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 2960 4108 5222 6606 6874 1632
Size: 559 769 1148 1114 1384 268 1917
Fragments arranged by size:
1917 1384 1148 1114 769 559 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6962
AflIII A'CryG_T
Cuts at: 1203 6058 7035 1203
Size: 4855 977 1327
Fragments arranged by size:
4855 1327 977
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 5888 5888
Size: 7159
AleI CACnn'nnGTG
Cuts at: 1166 3411 1166
Size: 2245 4914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7159
AlwNI CAG_nnn'CTG
Cuts at: 2107 4902 6474 2107
Size: 2795 1572 2792
Fragments arranged by size:
2795 2792 1572
ApaLI G'TGCA_C
Cuts at: 3068 5216 6372 3068
Size: 2148 1156 3855
Fragments arranged by size:
3855 2148 1156
ApoI r'AATT_y
Cuts at: 1493 2249 2525 2771 2846 3486 3698 3906 4118
Size: 756 276 246 75 640 212 208 212
Cuts at: 4118 4369 4468 4694 4846 6955 1493
Size: 251 99 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 640 276 251 246 226
212 212 208 152 99 75
AseI AT'TA_AT
Cuts at: 160 1032 5713 160
Size: 872 4681 1606
Fragments arranged by size:
4681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 4484 1870
Size: 73 2541 4545
Fragments arranged by size:
4545 2541 73
BamHI G'GATC_C
Cuts at: 4489 4856 4489
Size: 367 6792
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4480 5935 618
Size: 325 217 627 2693 1455 1842
Fragments arranged by size:
2693 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 4162 4478 1391
Size: 737 2034 316 4072
Fragments arranged by size:
4072 2034 737 316
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4333 928
Size: 971 2434 3754
Fragments arranged by size:
3754 2434 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5350 5384 1903
Size: 34 3413 34 3678
Fragments arranged by size:
3678 3413 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5051 6261 5051
Size: 1210 5949
BclI T'GATC_A
Cuts at: 1548 2284 1548
Size: 736 6423
BfrBI ATG'CAT
Cuts at: 3521 3521
Size: 7159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5770 136
Size: 107 122 71 631 605 4098 1525
Fragments arranged by size:
4098 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7154 7154
Size: 7159
BlpI GC'TnA_GC
Cuts at: 4576 4576
Size: 7159
Bme1580I G_kGCm'C
Cuts at: 1790 3072 5220 6376 1790
Size: 1282 2148 1156 2573
Fragments arranged by size:
2573 2148 1282 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4890 5848 442
Size: 416 53 83 399 24 3473 958 1753
Fragments arranged by size:
3473 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 5819 1239
Size: 929 3651 2579
Fragments arranged by size:
3651 2579 929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2565 3455 5284 6149 6447 2565
Size: 890 1829 865 298 3277
Fragments arranged by size:
3277 1829 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5822 882
Size: 617 4323 2219
Fragments arranged by size:
4323 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4456 4950 493
Size: 711 3252 494 2702
Fragments arranged by size:
3252 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7153 7153
Size: 7159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4966 5348 5983 275
Size: 53 83 186 936 3433 382 635 1451
Fragments arranged by size:
3433 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5592 5990 6264 6411 6822 5592
Size: 398 274 147 411 5929
Fragments arranged by size:
5929 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6934
Fragments arranged by size:
6934 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2314 2645 1186
Size: 922 206 331 5700
Fragments arranged by size:
5700 922 331 206
BseYI C'CCAG_C
Cuts at: 6362 6858 6362
Size: 496 6663
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6586
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4609 5370 6398 1540
Size: 275 186 2608 761 1028 2301
Fragments arranged by size:
2608 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2670 3072 5220 5305 6376 2670
Size: 402 2148 85 1071 3453
Fragments arranged by size:
3453 2148 1071 402 85
BsmI GAATG_Cn'
Cuts at: 3182 4106 4670 4763 3182
Size: 924 564 93 5578
Fragments arranged by size:
5578 924 564 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2670 3072 4162 4478 5220 5305
Size: 399 338 542 402 1090 316 742 85
Cuts at: 5305 6376 1391
Size: 1071 2174
Fragments arranged by size:
2174 1090 1071 742 542 402 399 338
316 85
BspEI T'CCGG_A
Cuts at: 6822 6822
Size: 7159
BspHI T'CATG_A
Cuts at: 2626 3769 5048 6778 2626
Size: 1143 1279 1730 3007
Fragments arranged by size:
3007 1730 1279 1143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4514 844
Size: 3670 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5046 1394
Size: 429 3223 3507
Fragments arranged by size:
3507 3223 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3187 4111 5654 5828 65
Size: 1183 1939 924 1543 174 1396
Fragments arranged by size:
1939 1543 1396 1183 924 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5803 5990 1700
Size: 110 3993 187 2869
Fragments arranged by size:
3993 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3979 4315 96
Size: 3883 336 2940
Fragments arranged by size:
3883 2940 336
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 5219 6231 5219
Size: 1012 6147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7159
BstBI TT'CG_AA
Cuts at: 2007 5963 2007
Size: 3956 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7159
BstYI r'GATC_y
Cuts at: 1972 4489 4856 5241 5258 6710 6796 6808 7154
Size: 2517 367 385 17 1452 86 12 346
Cuts at: 7154 1972
Size: 1977
Fragments arranged by size:
2517 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2231 513
Size: 552 1166 5441
Fragments arranged by size:
5441 1166 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6420
Fragments arranged by size:
6420 683 56
BtsI GCAGTG_nn'
Cuts at: 4675 5469 5489 4675
Size: 794 20 6345
Fragments arranged by size:
6345 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3169 3204 4093 4128 529
Size: 35 2605 35 889 35 3560
Fragments arranged by size:
3560 2605 889 35 35 35
DraI TTT'AAA
Cuts at: 2065 4463 2065
Size: 2398 4761
DraIII CAC_nnn'GTG
Cuts at: 4418 4418
Size: 7159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6166 809
Size: 507 4850 1802
Fragments arranged by size:
4850 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4606 5495 7124
Size: 54 1425 50 275 18 2776 889 1629
Cuts at: 7124 8
Size: 43
Fragments arranged by size:
2776 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4606 1537
Size: 275 2794 4090
Fragments arranged by size:
4090 2794 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 2726 2742 5089 1353
Size: 867 506 16 2347 3423
Fragments arranged by size:
3423 2347 867 506 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5746 6120 6266 387
Size: 1064 138 4157 374 146 1280
Fragments arranged by size:
4157 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2191 2960 4108 5222 5819 6606
Size: 393 536 23 769 1148 1114 597 787
Cuts at: 6606 6874 1239
Size: 268 1524
Fragments arranged by size:
1524 1148 1114 787 769 597 536 393
268 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2708
Size: 7159
EcoRI G'AATT_C
Cuts at: 2249 3486 3906 4468 4846 2249
Size: 1237 420 562 378 4562
Fragments arranged by size:
4562 1237 562 420 378
EcoRV GAT'ATC
Cuts at: 2820 2820
Size: 7159
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 6306 1678
Size: 4628 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2269 2301 2783
Size: 32 135 32 470 32 195 32 482
Cuts at: 2783 2815 5800 5832 5874 5906 1373
Size: 32 2985 32 42 32 2626
Fragments arranged by size:
2985 2626 482 470 195 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3717 4503 4749 669
Size: 988 281 1779 786 246 3079
Fragments arranged by size:
3079 1779 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 2263 3027 3111 3951 748
Size: 1515 764 84 840 3956
Fragments arranged by size:
3956 1515 840 764 84
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KpnI G_GTAC'C
Cuts at: 4484 4484
Size: 7159
MfeI C'AATT_G
Cuts at: 4758 4758
Size: 7159
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6273 6457 1305
Size: 4968 184 2007
Fragments arranged by size:
4968 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5337
Fragments arranged by size:
5337 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3411 3774 5117 5476 5635 518
Size: 648 2245 363 1343 359 159 2042
Fragments arranged by size:
2245 2042 1343 648 363 359 159
MspA1I CmG'CkG
Cuts at: 1606 2282 4575 5252 6400 6645 1606
Size: 676 2293 677 1148 245 2120
Fragments arranged by size:
2293 2120 1148 677 676 245
NcoI C'CATG_G
Cuts at: 513 1065 2231 513
Size: 552 1166 5441
Fragments arranged by size:
5441 1166 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7159
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NsiI A_TGCA'T
Cuts at: 3523 3523
Size: 7159
NspI r_CATG'y
Cuts at: 1132 1229 3525 3684 4281 4517 6062 1132
Size: 97 2296 159 597 236 1545 2229
Fragments arranged by size:
2296 2229 1545 597 236 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7159
PciI A'CATG_T
Cuts at: 6058 6058
Size: 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7159
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5228 5260 6741 6773 5228
Size: 32 1481 32 5614
Fragments arranged by size:
5614 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2115 1613
Size: 502 6657
PsiI TTA'TAA
Cuts at: 2766 4729 2766
Size: 1963 5196
PstI C_TGCA'G
Cuts at: 830 4511 830
Size: 3681 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
PvuII CAG'CTG
Cuts at: 2282 2282
Size: 7159
SalI G'TCGA_C
Cuts at: 1936 4501 1936
Size: 2565 4594
SapI GCTCTTCn'nnn_
Cuts at: 2220 2220
Size: 7159
SbfI CC_TGCA'GG
Cuts at: 4511 4511
Size: 7159
ScaI AGT'ACT
Cuts at: 5407 7119 5407
Size: 1712 5447
SfcI C'TryA_G
Cuts at: 826 1045 2155 4507 5642 6323 6514 826
Size: 219 1110 2352 1135 681 191 1471
Fragments arranged by size:
2352 1471 1135 1110 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 4486 1872
Size: 2614 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2580 3434 5263 6164 6426 820
Size: 197 926 637 854 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 854 637 262 197
SnaBI TAC'GTA
Cuts at: 493 4456 4950 493
Size: 3963 494 2702
Fragments arranged by size:
3963 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 4517 1132
Size: 3385 3774
SspI AAT'ATT
Cuts at: 5 52 2557 5083 5
Size: 47 2505 2526 2081
Fragments arranged by size:
2526 2505 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7159
StyI C'CwwG_G
Cuts at: 513 1065 2231 4598 513
Size: 552 1166 2367 3074
Fragments arranged by size:
3074 2367 1166 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 5186 5203 5356 5541 5986 325
Size: 1255 522 3084 17 153 185 445 1498
Fragments arranged by size:
3084 1498 1255 522 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3391 3643 3979
Size: 274 80 33 51 1443 1414 252 336
Cuts at: 3979 4147 4315 5405 7117 96
Size: 168 168 1090 1712 138
Fragments arranged by size:
1712 1443 1414 1090 336 274 252 168
168 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7119 7151 7119
Size: 32 7127
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7159
XbaI T'CTAG_A
Cuts at: 4495 4495
Size: 7159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 4484 1870
Size: 2614 4545
XmnI GAAnn'nnTTC
Cuts at: 2945 3029 3533 3617 4121 4289 5288 2945
Size: 84 504 84 504 168 999 4816
Fragments arranged by size:
4816 999 504 504 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4967 276
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI