Restriction map for
pFN21ASDA0064
(Circular) (Six-base) MAPSORT of: pFN21ASDA0064.seq Check: 788 from: 1 to: 6139
pFN21ASDA0064 6139 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 4202 5586 5854 1632
Size: 780 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 780 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3164 1184
Size: 1980 4159
AlwNI CAG_nnn'CTG
Cuts at: 2143 2818 3882 5454 2143
Size: 675 1064 1572 2828
Fragments arranged by size:
2828 1572 1064 675
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3297 3464 1870
Size: 73 1354 167 4545
Fragments arranged by size:
4545 1354 167 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2488 2521 2488
Size: 33 6106
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 4915 618
Size: 325 217 627 1673 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2913 3458 1391
Size: 1522 545 4072
Fragments arranged by size:
4072 1522 545
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5168
BbvCI CC'TCA_GC
Cuts at: 2232 2232
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2261 2295 4330 4364 1903
Size: 34 324 34 2035 34 3678
Fragments arranged by size:
3678 2035 324 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2489 4031 5241 2489
Size: 1542 1210 3387
Fragments arranged by size:
3387 1542 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 2855 2934
Size: 107 122 71 631 605 412 771 79
Cuts at: 2934 4750 136
Size: 1816 1525
Fragments arranged by size:
1816 1525 771 631 605 412 122 107
79 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 2390 2912 3556 2390
Size: 522 644 4973
Fragments arranged by size:
4973 644 522
Bme1580I G_kGCm'C
Cuts at: 1790 2338 4200 5356 1790
Size: 548 1862 1156 2573
Fragments arranged by size:
2573 1862 1156 548
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3006 3870 4828
Size: 416 53 83 399 24 1589 864 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1753 1589 958 864 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 4799 1239
Size: 1232 2328 2579
Fragments arranged by size:
2579 2328 1232
Bpu10I CC'TnA_GC
Cuts at: 2232 2624 2232
Size: 392 5747
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3299 3304 4264 5129 5427 3299
Size: 5 960 865 298 4011
Fragments arranged by size:
4011 960 865 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2083 2101 3436 3930 493
Size: 711 879 18 1335 494 2702
Fragments arranged by size:
2702 1335 879 711 494 18
BsaBI GATnn'nnATC
Cuts at: 3140 3426 6133 3140
Size: 286 2707 3146
Fragments arranged by size:
3146 2707 286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 2986 1186
Size: 1074 726 4339
Fragments arranged by size:
4339 1074 726
BseYI C'CCAG_C
Cuts at: 3362 5342 5838 3362
Size: 1980 496 3663
Fragments arranged by size:
3663 1980 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2135 1163
Size: 573 363 36 5167
Fragments arranged by size:
5167 573 363 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2913 4200 4285 5356 2913
Size: 1287 85 1071 3696
Fragments arranged by size:
3696 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 2195 3650 3743 2195
Size: 1455 93 4591
Fragments arranged by size:
4591 1455 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3229 3229
Size: 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2338 2913 3458 4200 4285 5356 1391
Size: 399 548 575 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 548 545 399 85
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 3081 3132 4028 5758 3081
Size: 51 896 1730 3462
Fragments arranged by size:
3462 1730 896 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2344 2697 3494 844
Size: 1500 353 797 3489
Fragments arranged by size:
3489 1500 797 353
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2291 3005 4634 4808 65
Size: 1183 1043 714 1629 174 1396
Fragments arranged by size:
1629 1396 1183 1043 714 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2754 3093 3213 4783 4970 1700
Size: 110 944 339 120 1570 187 2869
Fragments arranged by size:
2869 1570 944 339 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 2153 3022 4199 5211 2153
Size: 869 1177 1012 3081
Fragments arranged by size:
3081 1177 1012 869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2979 794
Size: 2185 3954
BstBI TT'CG_AA
Cuts at: 2007 4943 2007
Size: 2936 3203
BstEII G'GTnAC_C
Cuts at: 1184 2737 2965 2989 3106 1184
Size: 1553 228 24 117 4217
Fragments arranged by size:
4217 1553 228 117 24
BstYI r'GATC_y
Cuts at: 1972 2473 2730 3427 3469 3836 4221 4238 5690
Size: 501 257 697 42 367 385 17 1452
Cuts at: 5690 5776 5788 6134 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 697 501 385 367 346 257
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3156 3391 513
Size: 552 2091 235 3261
Fragments arranged by size:
3261 2091 552 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2992 487
Size: 683 56 1766 3634
Fragments arranged by size:
3634 1766 683 56
BtsI GCAGTG_nn'
Cuts at: 3264 3272 3655 4449 4469 3264
Size: 8 383 794 20 4934
Fragments arranged by size:
4934 794 383 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3586 4475
Size: 54 1425 50 275 18 1105 651 889
Cuts at: 4475 6104 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1105 889 651 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 4069 1353
Size: 1271 1445 3423
Fragments arranged by size:
3423 1445 1271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 2915 4726 5100 5246 387
Size: 1064 138 476 850 1811 374 146 1280
Fragments arranged by size:
1811 1280 1064 850 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 2911 2911
Size: 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 2471 4202 4799 5586 5854 1239
Size: 393 780 59 1731 597 787 268 1524
Fragments arranged by size:
1731 1524 787 780 597 393 268 59
EcoO109I rG'GnC_Cy
Cuts at: 2356 2356
Size: 6139
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 5286 1678
Size: 3608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 4780 4812 4854
Size: 32 135 32 785 32 2391 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 2391 785 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 3483 3729 669
Size: 988 281 478 1067 246 3079
Fragments arranged by size:
3079 1067 988 478 281 246
HindIII A'AGCT_T
Cuts at: 748 2210 748
Size: 1462 4677
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5253 5437 1305
Size: 3948 184 2007
Fragments arranged by size:
3948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2937 10
Size: 54 1425 343 1105 3212
Fragments arranged by size:
3212 1425 1105 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2103 2972 4097 4456 4615 518
Size: 648 933 4 869 1125 359 159 2042
Fragments arranged by size:
2042 1125 933 869 648 359 159 4
MspA1I CmG'CkG
Cuts at: 1606 2058 2067 3555 4232 5380 5625 1606
Size: 452 9 1488 677 1148 245 2120
Fragments arranged by size:
2120 1488 1148 677 452 245 9
NaeI GCC'GGC
Cuts at: 3215 3215
Size: 6139
NcoI C'CATG_G
Cuts at: 513 1065 3156 513
Size: 552 2091 3496
Fragments arranged by size:
3496 2091 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 3213 3213
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 2272 2977 3497 5042 1132
Size: 97 1043 705 520 1545 2229
Fragments arranged by size:
2229 1545 1043 705 520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 2297 1108
Size: 1035 154 4950
Fragments arranged by size:
4950 1035 154
PfoI T'CCnGG_A
Cuts at: 2448 2448
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PmlI CAC'GTG
Cuts at: 2101 2101
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2356 2356
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 2922 3491 830
Size: 2092 569 3478
Fragments arranged by size:
3478 2092 569
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
SacI G_AGCT'C
Cuts at: 2913 2913
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 2131 2457 2918 3098 3487 4622 5303
Size: 219 1086 326 461 180 389 1135 681
Cuts at: 5303 5494 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1086 681 461 389 326 219
191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 3213 1700
Size: 1513 4626
SmaI CCC'GGG
Cuts at: 1872 3299 3466 1872
Size: 1427 167 4545
Fragments arranged by size:
4545 1427 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3278 3319 4243 5144 5406 820
Size: 197 926 1335 41 924 901 262 1553
Fragments arranged by size:
1553 1335 926 924 901 262 197 41
SnaBI TAC'GTA
Cuts at: 493 3436 3930 493
Size: 2943 494 2702
Fragments arranged by size:
2943 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 2977 3497 1132
Size: 1845 520 3774
Fragments arranged by size:
3774 1845 520
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6139
StyI C'CwwG_G
Cuts at: 513 1065 2376 2742 3156 3578 513
Size: 552 1311 366 414 422 3074
Fragments arranged by size:
3074 1311 552 422 414 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2715 4166 4183 4336 4521 4966 325
Size: 1255 1135 1451 17 153 185 445 1498
Fragments arranged by size:
1498 1451 1255 1135 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 4385 6097
Size: 274 80 33 51 1443 288 2120 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
2120 1712 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2372 6099 6131 2340
Size: 32 3727 32 2348
Fragments arranged by size:
3727 2348 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2660 1316
Size: 1344 4795
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3297 3464 1870
Size: 1427 167 4545
Fragments arranged by size:
4545 1427 167
XmnI GAAnn'nnTTC
Cuts at: 2213 2795 4268 2213
Size: 582 1473 4084
Fragments arranged by size:
4084 1473 582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI