Restriction map for
pFN21ASDA0057
(Circular) (Six-base) MAPSORT of: pFN21ASDA0057.seq Check: 7225 from: 1 to: 5839
pFN21ASDA0057 5839 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2044 3902 5286 5554 1632
Size: 412 1858 1384 268 1917
Fragments arranged by size:
1917 1858 1384 412 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 2381 4568 2381
Size: 2187 3652
AleI CACnn'nnGTG
Cuts at: 1166 2402 1166
Size: 1236 4603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 5807
AlwNI CAG_nnn'CTG
Cuts at: 2763 2901 3582 5154 2763
Size: 138 681 1572 3448
Fragments arranged by size:
3448 1572 681 138
ApaLI G'TGCA_C
Cuts at: 2219 2483 3896 5052 2219
Size: 264 1413 1156 3006
Fragments arranged by size:
3006 1413 1156 264
ApoI r'AATT_y
Cuts at: 1493 2583 3148 3374 3526 5635 1493
Size: 1090 565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1090 565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 2864 3164 1870
Size: 73 921 300 4545
Fragments arranged by size:
4545 921 300 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3158 1391
Size: 1767 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2475 928
Size: 971 576 4292
Fragments arranged by size:
4292 971 576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2432 3731 4941 2432
Size: 1299 1210 3330
Fragments arranged by size:
3330 1299 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2661 2816 2930
Size: 107 122 71 631 605 989 155 114
Cuts at: 2930 4450 136
Size: 1520 1525
Fragments arranged by size:
1525 1520 989 631 605 155 122 114
107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2223 2487 3900 5056 1790
Size: 433 264 1413 1156 2573
Fragments arranged by size:
2573 1413 1156 433 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2657
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2582 2652 4499 1239
Size: 1343 70 1847 2579
Fragments arranged by size:
2579 1847 1343 70
Bpu10I CC'TnA_GC
Cuts at: 2550 2651 2550
Size: 101 5738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3107 3964 4829 5127 3107
Size: 857 865 298 3819
Fragments arranged by size:
3819 865 857 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 4502 882
Size: 617 708 2295 2219
Fragments arranged by size:
2295 2219 708 617
BsaAI yAC'GTr
Cuts at: 493 1204 2422 3136 3630 493
Size: 711 1218 714 494 2702
Fragments arranged by size:
2702 1218 714 711 494
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3086 3116 1178
Size: 30 165 30 1683 30 3901
Fragments arranged by size:
3901 1683 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5839
BseYI C'CCAG_C
Cuts at: 2961 3016 5042 5538 2961
Size: 55 2026 496 3262
Fragments arranged by size:
3262 2026 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2522 1163
Size: 573 786 4480
Fragments arranged by size:
4480 786 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2223 2487 3900 3985 5056 2115
Size: 108 264 1413 85 1071 2898
Fragments arranged by size:
2898 1413 1071 264 108 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2223 2487 3158 3900 3985 5056
Size: 399 325 108 264 671 742 85 1071
Cuts at: 5056 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 671 399 325 264 108
85
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2056 3726 1394
Size: 429 233 1670 3507
Fragments arranged by size:
3507 1670 429 233
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2901 794
Size: 2107 3732
BstBI TT'CG_AA
Cuts at: 2007 4643 2007
Size: 2636 3203
BstEII G'GTnAC_C
Cuts at: 1184 2782 3062 1184
Size: 1598 280 3961
Fragments arranged by size:
3961 1598 280
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834
Size: 1197 367 385 17 1452 86 12 346
Cuts at: 5834 1972
Size: 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2238 2238
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2022 3107 513
Size: 552 957 1085 3245
Fragments arranged by size:
3245 1085 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 3355 4149 4169 3355
Size: 794 20 5025
Fragments arranged by size:
5025 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2817 3286 4175
Size: 54 1425 50 275 18 987 469 889
Cuts at: 4175 5804 8
Size: 1629 43
Fragments arranged by size:
1629 1425 987 889 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2708 2726 2798 3769 1353
Size: 710 645 18 72 971 3423
Fragments arranged by size:
3423 971 710 645 72 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2582 2652 3902 4499 5286 5554
Size: 393 412 538 70 1250 597 787 268
Cuts at: 5554 1239
Size: 1524
Fragments arranged by size:
1524 1250 787 597 538 412 393 268
70
EcoO109I rG'GnC_Cy
Cuts at: 2242 2242
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5839
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 2950 4986 1678
Size: 1272 2036 2531
Fragments arranged by size:
2531 2036 1272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2367 2399 4480 4512 4554
Size: 32 135 32 795 32 2081 32 42
Cuts at: 4554 4586 1373
Size: 32 2626
Fragments arranged by size:
2626 2081 795 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 10
Size: 54 1425 343 987 3030
Fragments arranged by size:
3030 1425 987 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2192 2402 3072 3797 4156 4315
Size: 648 919 107 210 670 725 359 159
Cuts at: 4315 518
Size: 2042
Fragments arranged by size:
2042 919 725 670 648 359 210 159
107
MspA1I CmG'CkG
Cuts at: 1606 2380 2524 2965 3255 3932 5080 5325 1606
Size: 774 144 441 290 677 1148 245 2120
Fragments arranged by size:
2120 1148 774 677 441 290 245 144
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4330
Fragments arranged by size:
4330 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2291 2483 3197 4742 1132
Size: 97 1062 192 714 1545 2229
Fragments arranged by size:
2229 1545 1062 714 192 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 3086 3118 3908 3940 5421 5453 2738
Size: 32 316 32 790 32 1481 32 3124
Fragments arranged by size:
3124 1481 790 316 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2242
Size: 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2685 3191 830
Size: 1855 506 3478
Fragments arranged by size:
3478 1855 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2380 2524 2965 2380
Size: 144 441 5254
Fragments arranged by size:
5254 441 144
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2063 2063
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 2687 4087 5799 2687
Size: 1400 1712 2727
Fragments arranged by size:
2727 1712 1400
SexAI A'CCwGG_T
Cuts at: 2244 2616 2244
Size: 372 5467
SfcI C'TryA_G
Cuts at: 826 1045 2199 2681 3187 4322 5003 5194 826
Size: 219 1154 482 506 1135 681 191 1471
Fragments arranged by size:
1471 1154 1135 681 506 482 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3122 3943 4844 5106 820
Size: 197 926 1179 821 901 262 1553
Fragments arranged by size:
1553 1179 926 901 821 262 197
SnaBI TAC'GTA
Cuts at: 493 3136 3630 493
Size: 2643 494 2702
Fragments arranged by size:
2702 2643 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 2291 3197 1132
Size: 1159 906 3774
Fragments arranged by size:
3774 1159 906
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 2933 1517
Size: 1416 4423
StyI C'CwwG_G
Cuts at: 513 1065 2022 3278 513
Size: 552 957 1256 3074
Fragments arranged by size:
3074 1256 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3040 3866 3883 4036 4221 4666 325
Size: 1255 1460 826 17 153 185 445 1498
Fragments arranged by size:
1498 1460 1255 826 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2685 4085
Size: 274 80 33 51 1443 327 381 1400
Cuts at: 4085 5797 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1400 381 327 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5799 5831 5799
Size: 32 5807
Tth111I GACn'n_nGTC
Cuts at: 1316 2246 1316
Size: 930 4909
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2460 2460
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 2507 2753 3968 2507
Size: 246 1215 4378
Fragments arranged by size:
4378 1215 246
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI