Restriction map for
pFN21ASDA0053
(Circular) (Six-base) MAPSORT of: pFN21ASDA0053.seq Check: 1322 from: 1 to: 6643
pFN21ASDA0053 6643 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4453 278
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3986 1937
Size: 2049 4594
Acc65I G'GTAC_C
Cuts at: 3964 3964
Size: 6643
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 3172 3177 4706 6090 6358 1632
Size: 548 992 5 1529 1384 268 1917
Fragments arranged by size:
1917 1529 1384 992 548 268 5
AfeI AGC'GCT
Cuts at: 2480 3845 2480
Size: 1365 5278
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 5542 6519 1203
Size: 4339 977 1327
Fragments arranged by size:
4339 1327 977
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 2681 1166
Size: 1515 5128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2115 1184
Size: 931 5712
AlwNI CAG_nnn'CTG
Cuts at: 4386 5958 4386
Size: 1572 5071
ApaLI G'TGCA_C
Cuts at: 2684 3552 4700 5856 2684
Size: 868 1148 1156 3471
Fragments arranged by size:
3471 1156 1148 868
ApoI r'AATT_y
Cuts at: 1493 2432 2510 3168 3404 3899 3952 4178 4330
Size: 939 78 658 236 495 53 226 152
Cuts at: 4330 6439 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 939 658 495 236 226 152
78 53
AseI AT'TA_AT
Cuts at: 160 1032 5197 160
Size: 872 4165 1606
Fragments arranged by size:
4165 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 3849 3968 1870
Size: 73 1906 119 4545
Fragments arranged by size:
4545 1906 119 73
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2527 3368 3964 5419 618
Size: 325 217 627 740 841 596 1455 1842
Fragments arranged by size:
1842 1455 841 740 627 596 325 217
BanII G_rGCy'C
Cuts at: 1391 2736 3962 1391
Size: 1345 1226 4072
Fragments arranged by size:
4072 1345 1226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 3165 928
Size: 971 1086 180 4406
Fragments arranged by size:
4406 1086 971 180
BbvCI CC'TCA_GC
Cuts at: 2210 2210
Size: 6643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2966 3000 4834 4868 1903
Size: 34 1029 34 1834 34 3678
Fragments arranged by size:
3678 1834 1029 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4535 5745 4535
Size: 1210 5433
BclI T'GATC_A
Cuts at: 1548 2991 3036 1548
Size: 1443 45 5155
Fragments arranged by size:
5155 1443 45
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5254 136
Size: 107 122 71 631 605 3582 1525
Fragments arranged by size:
3582 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3390 6638 3390
Size: 3248 3395
BlpI GC'TnA_GC
Cuts at: 2808 4060 2808
Size: 1252 5391
Bme1580I G_kGCm'C
Cuts at: 1790 2532 2688 3447 3556 4704 5860 1790
Size: 742 156 759 109 1148 1156 2573
Fragments arranged by size:
2573 1156 1148 759 742 156 109
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4374 5332
Size: 416 53 83 399 24 2207 750 958
Cuts at: 5332 442
Size: 1753
Fragments arranged by size:
2207 1753 958 750 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 3021 2677
Size: 344 6299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2585 2767 3105 3657 3809 5303 1239
Size: 914 432 182 338 552 152 1494 2579
Fragments arranged by size:
2579 1494 914 552 432 338 182 152
Bpu10I CC'TnA_GC
Cuts at: 2210 2864 3429 2210
Size: 654 565 5424
Fragments arranged by size:
5424 654 565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3447 3755 4768 5633 5931 3447
Size: 308 1013 865 298 4159
Fragments arranged by size:
4159 1013 865 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2715 3036 5306 882
Size: 617 1216 321 2270 2219
Fragments arranged by size:
2270 2219 1216 617 321
BsaAI yAC'GTr
Cuts at: 493 1204 3940 4434 493
Size: 711 2736 494 2702
Fragments arranged by size:
2736 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6637 6637
Size: 6643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4450 4832 5467 275
Size: 53 83 186 936 2917 382 635 1451
Fragments arranged by size:
2917 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3227 3253 3336 5076 5474 5748 5895 6306 3227
Size: 26 83 1740 398 274 147 411 3564
Fragments arranged by size:
3564 1740 411 398 274 147 83 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6418
Fragments arranged by size:
6418 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 2997 1186
Size: 1249 562 4832
Fragments arranged by size:
4832 1249 562
BseYI C'CCAG_C
Cuts at: 2321 2648 3144 5846 6342 2321
Size: 327 496 2702 496 2622
Fragments arranged by size:
2702 2622 496 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2571 1163
Size: 573 835 5235
Fragments arranged by size:
5235 835 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 4093 4854 5882 1540
Size: 275 186 574 1518 761 1028 2301
Fragments arranged by size:
2301 1518 1028 761 574 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 2688 3556 4704 4789 5860 2571
Size: 117 868 1148 85 1071 3354
Fragments arranged by size:
3354 1148 1071 868 117 85
BsmI GAATG_Cn'
Cuts at: 2794 4154 4247 2794
Size: 1360 93 5190
Fragments arranged by size:
5190 1360 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2628 2628
Size: 6643
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2532 2571 2688 2736 3447 3556 3962
Size: 399 742 39 117 48 711 109 406
Cuts at: 3962 4704 4789 5860 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 742 711 406 399 117
109 85 48 39
BspEI T'CCGG_A
Cuts at: 3253 6306 3253
Size: 3053 3590
BspHI T'CATG_A
Cuts at: 2994 4532 6262 2994
Size: 1538 1730 3375
Fragments arranged by size:
3375 1730 1538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2868 3998 844
Size: 2024 1130 3489
Fragments arranged by size:
3489 2024 1130
BsrBI CCG'CTC
Cuts at: 1394 1823 2656 4530 1394
Size: 429 833 1874 3507
Fragments arranged by size:
3507 1874 833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5138 5312 65
Size: 1183 3890 174 1396
Fragments arranged by size:
3890 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5287 5474 1700
Size: 110 3477 187 2869
Fragments arranged by size:
3477 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2285 96
Size: 2189 4454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 2844 4703 5715 2844
Size: 1859 1012 3772
Fragments arranged by size:
3772 1859 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 2007 5447 2007
Size: 3440 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2149 2355 2539 3294 2131
Size: 18 206 184 755 5480
Fragments arranged by size:
5480 755 206 184 18
BstYI r'GATC_y
Cuts at: 1972 3012 3390 3973 4340 4725 4742 6194 6280
Size: 1040 378 583 367 385 17 1452 86
Cuts at: 6280 6292 6638 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1040 583 385 378 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2438 2438
Size: 6643
BtgI C'CryG_G
Cuts at: 513 1065 2736 513
Size: 552 1671 4420
Fragments arranged by size:
4420 1671 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3258 487
Size: 683 56 2032 3872
Fragments arranged by size:
3872 2032 683 56
BtsI GCAGTG_nn'
Cuts at: 4159 4953 4973 4159
Size: 794 20 5829
Fragments arranged by size:
5829 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3397 3947 3397
Size: 550 6093
DraIII CAC_nnn'GTG
Cuts at: 2462 2692 2462
Size: 230 6413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2387 3050 5650 809
Size: 507 1071 663 2600 1802
Fragments arranged by size:
2600 1802 1071 663 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2473 2572
Size: 54 1425 50 275 18 567 76 99
Cuts at: 2572 4090 4979 6608 8
Size: 1518 889 1629 43
Fragments arranged by size:
1629 1518 1425 889 567 275 99 76
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2572 4090 1537
Size: 275 760 1518 4090
Fragments arranged by size:
4090 1518 760 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 2577 3062 3565 3636 3860 4573 1353
Size: 885 339 485 503 71 224 713 3423
Fragments arranged by size:
3423 885 713 503 485 339 224 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2426 5230 5604 5750 387
Size: 1064 138 837 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 837 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2180 2585 2767 3105 3172 3177
Size: 393 521 27 405 182 338 67 5
Cuts at: 3177 3657 3809 4706 5303 6090 6358 1239
Size: 480 152 897 597 787 268 1524
Fragments arranged by size:
1524 897 787 597 521 480 405 393
338 268 182 152 67 27 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3737 3737
Size: 6643
EcoO109I rG'GnC_Cy
Cuts at: 3531 3531
Size: 6643
EcoRI G'AATT_C
Cuts at: 3404 3952 4330 3404
Size: 548 378 5717
Fragments arranged by size:
5717 548 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 6643
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 2482 3847 5790 1678
Size: 804 1365 1943 2531
Fragments arranged by size:
2531 1943 1365 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 5284 5316 5358
Size: 32 135 32 702 32 2978 32 42
Cuts at: 5358 5390 1373
Size: 32 2626
Fragments arranged by size:
2978 2626 702 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2914 3987 4233 669
Size: 988 281 976 1073 246 3079
Fragments arranged by size:
3079 1073 988 976 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6643
HpaI GTT'AAC
Cuts at: 2914 4233 2914
Size: 1319 5324
KpnI G_GTAC'C
Cuts at: 3968 3968
Size: 6643
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5757 5941 1305
Size: 4452 184 2007
Fragments arranged by size:
4452 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2475 10
Size: 54 1425 343 567 76 4178
Fragments arranged by size:
4178 1425 567 343 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2537 2681 4601 4960 5119 518
Size: 648 981 390 144 1920 359 159 2042
Fragments arranged by size:
2042 1920 981 648 390 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2131 2629 2899 4059 4736 5884 6129 1606
Size: 525 498 270 1160 677 1148 245 2120
Fragments arranged by size:
2120 1160 1148 677 525 498 270 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6091
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6643
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 2272 4001 5546 1132
Size: 97 1043 1729 1545 2229
Fragments arranged by size:
2229 1729 1545 1043 97
PasI CC'CwG_GG
Cuts at: 1348 2260 3276 3536 1348
Size: 912 1016 260 4455
Fragments arranged by size:
4455 1016 912 260
PciI A'CATG_T
Cuts at: 5542 5542
Size: 6643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 1108
Size: 1374 5269
PfoI T'CCnGG_A
Cuts at: 2562 2562
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3445 3477 4712 4744 6225 6257 3445
Size: 32 1235 32 1481 32 3831
Fragments arranged by size:
3831 1481 1235 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3531 3531
Size: 6643
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 4213 4213
Size: 6643
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 6611
PstI C_TGCA'G
Cuts at: 830 2879 3814 3995 830
Size: 2049 935 181 3478
Fragments arranged by size:
3478 2049 935 181
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
PvuII CAG'CTG
Cuts at: 2629 2899 2629
Size: 270 6373
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SanDI GG'GwC_CC
Cuts at: 3531 3531
Size: 6643
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 4891 6603 4891
Size: 1712 4931
SexAI A'CCwGG_T
Cuts at: 2616 2616
Size: 6643
SfcI C'TryA_G
Cuts at: 826 1045 2058 2854 2875 3138 3810 3991 5126
Size: 219 1013 796 21 263 672 181 1135
Cuts at: 5126 5807 5998 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1013 796 681 672 263 219
191 181 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 3851 3970 1872
Size: 1979 119 4545
Fragments arranged by size:
4545 1979 119
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3462 3734 4747 5648 5910 820
Size: 197 926 1519 272 1013 901 262 1553
Fragments arranged by size:
1553 1519 1013 926 901 272 262 197
SnaBI TAC'GTA
Cuts at: 493 3940 4434 493
Size: 3447 494 2702
Fragments arranged by size:
3447 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 4001 1132
Size: 2869 3774
SspI AAT'ATT
Cuts at: 5 52 4567 5
Size: 47 4515 2081
Fragments arranged by size:
4515 2081 47
StuI AGG'CCT
Cuts at: 1517 3878 1517
Size: 2361 4282
StyI C'CwwG_G
Cuts at: 513 1065 3069 3579 3879 4082 513
Size: 552 2004 510 300 203 3074
Fragments arranged by size:
3074 2004 552 510 300 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2021 2055 3944 4670 4687 4840 5025
Size: 1255 441 34 1889 726 17 153 185
Cuts at: 5025 5470 325
Size: 445 1498
Fragments arranged by size:
1889 1498 1255 726 445 441 185 153
34 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2285 4889 6601
Size: 274 80 33 51 1443 308 2604 1712
Cuts at: 6601 96
Size: 138
Fragments arranged by size:
2604 1712 1443 308 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6603 6635 6603
Size: 32 6611
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3979 3979
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 3849 3968 1870
Size: 1979 119 4545
Fragments arranged by size:
4545 1979 119
XmnI GAAnn'nnTTC
Cuts at: 2343 3344 4772 2343
Size: 1001 1428 4214
Fragments arranged by size:
4214 1428 1001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4451 276
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI
XcmI