Restriction map for
pFN21ASDA0050
(Circular) (Six-base) MAPSORT of: pFN21ASDA0050.seq Check: 8942 from: 1 to: 6949
pFN21ASDA0050 6949 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4292 1937
Size: 2355 4594
Acc65I G'GTAC_C
Cuts at: 2517 4270 2517
Size: 1753 5196
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3094 4056 4143 5012 6396 6664 1632
Size: 1462 962 87 869 1384 268 1917
Fragments arranged by size:
1917 1462 1384 962 869 268 87
AflII C'TTAA_G
Cuts at: 820 1017 2797 820
Size: 197 1780 4972
Fragments arranged by size:
4972 1780 197
AflIII A'CryG_T
Cuts at: 1203 2746 5848 6825 1203
Size: 1543 3102 977 1327
Fragments arranged by size:
3102 1543 1327 977
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6949
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 6917
AlwNI CAG_nnn'CTG
Cuts at: 2356 2492 2880 4692 6264 2356
Size: 136 388 1812 1572 3041
Fragments arranged by size:
3041 1812 1572 388 136
ApaI G_GGCC'C
Cuts at: 2470 3519 3742 3950 2470
Size: 1049 223 208 5469
Fragments arranged by size:
5469 1049 223 208
ApaLI G'TGCA_C
Cuts at: 5006 6162 5006
Size: 1156 5793
ApoI r'AATT_y
Cuts at: 1493 2267 2710 4258 4484 4636 6745 1493
Size: 774 443 1548 226 152 2109 1697
Fragments arranged by size:
2109 1697 1548 774 443 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 2508 3138 4026 4274 1870
Size: 73 565 630 888 248 4545
Fragments arranged by size:
4545 888 630 565 248 73
BamHI G'GATC_C
Cuts at: 4279 4646 4279
Size: 367 6582
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 2460 2517 2684 3624
Size: 325 217 627 382 291 57 167 940
Cuts at: 3624 4270 5725 618
Size: 646 1455 1842
Fragments arranged by size:
1842 1455 940 646 627 382 325 291
217 167 57
BanII G_rGCy'C
Cuts at: 1391 2244 2470 3158 3197 3519 3705 3742 3950
Size: 853 226 688 39 322 186 37 208
Cuts at: 3950 3987 4027 4268 1391
Size: 37 40 241 4072
Fragments arranged by size:
4072 853 688 322 241 226 208 186
40 39 37 37
BbeI G_GCGC'C
Cuts at: 2173 2464 2688 3628 2173
Size: 291 224 940 5494
Fragments arranged by size:
5494 940 291 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5978
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4841 6051 4841
Size: 1210 5739
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 5560 136
Size: 107 122 71 631 605 802 3086 1525
Fragments arranged by size:
3086 1525 802 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3471 6944 3471
Size: 3473 3476
BlpI GC'TnA_GC
Cuts at: 2651 4211 4366 2651
Size: 1560 155 5234
Fragments arranged by size:
5234 1560 155
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2470 2533 3240 3519 3651 3742 3950
Size: 360 320 63 707 279 132 91 208
Cuts at: 3950 5010 6166 1790
Size: 1060 1156 2573
Fragments arranged by size:
2573 1156 1060 707 360 320 279 208
132 91 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6949
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2153 2659 2683
Size: 416 53 83 399 24 736 506 24
Cuts at: 2683 3271 3829 4680 5638 442
Size: 588 558 851 958 1753
Fragments arranged by size:
1753 958 851 736 588 558 506 416
399 83 53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 3724 2523
Size: 1201 5748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 2890 3026 3887 5609 1239
Size: 1205 446 136 861 1722 2579
Fragments arranged by size:
2579 1722 1205 861 446 136
Bpu10I CC'TnA_GC
Cuts at: 3157 3157
Size: 6949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 3706 5074 5939 6237 2039
Size: 1667 1368 865 298 2751
Fragments arranged by size:
2751 1667 1368 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 2159 3402 5612 882
Size: 617 612 48 1243 2210 2219
Fragments arranged by size:
2219 2210 1243 617 612 48
BsaAI yAC'GTr
Cuts at: 493 1204 2749 3241 3565 4246 4740 493
Size: 711 1545 492 324 681 494 2702
Fragments arranged by size:
2702 1545 711 681 494 492 324
BsaBI GATnn'nnATC
Cuts at: 6943 6943
Size: 6949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2170 2461 2685 3625
Size: 53 83 186 936 637 291 224 940
Cuts at: 3625 4190 4756 5138 5773 275
Size: 565 566 382 635 1451
Fragments arranged by size:
1451 940 936 637 635 566 565 382
291 224 186 83 53
BsaWI w'CCGG_w
Cuts at: 3393 5382 5780 6054 6201 6612 3393
Size: 1989 398 274 147 411 3730
Fragments arranged by size:
3730 1989 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3358 3388 3560 3590 3798
Size: 30 165 30 1955 30 172 30 208
Cuts at: 3798 3828 1178
Size: 30 4299
Fragments arranged by size:
4299 1955 208 172 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2660 2795 3029 3890 1186
Size: 874 600 135 234 861 4245
Fragments arranged by size:
4245 874 861 600 234 135
BseYI C'CCAG_C
Cuts at: 2513 2680 2770 3448 3518 6152 6648 2513
Size: 167 90 678 70 2634 496 2814
Fragments arranged by size:
2814 2634 678 496 167 90 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2950 3107 3713 3938 1163
Size: 573 1214 157 606 225 4174
Fragments arranged by size:
4174 1214 606 573 225 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 5160 6188 1540
Size: 275 186 2398 761 1028 2301
Fragments arranged by size:
2398 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5010 5095 6166 5010
Size: 85 1071 5793
Fragments arranged by size:
5793 1071 85
BsmI GAATG_Cn'
Cuts at: 3342 4460 4553 3342
Size: 1118 93 5738
Fragments arranged by size:
5738 1118 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2966 4145 2966
Size: 1179 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2244 2470 2533 3158 3197 3240
Size: 399 360 94 226 63 625 39 43
Cuts at: 3240 3519 3651 3705 3742 3950 3987 4027 4268
Size: 279 132 54 37 208 37 40 241
Cuts at: 4268 5010 5095 6166 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 399 360 279 241
226 208 132 94 85 63 54 43
40 39 37 37
BspEI T'CCGG_A
Cuts at: 6612 6612
Size: 6949
BspHI T'CATG_A
Cuts at: 4225 4838 6568 4225
Size: 613 1730 4606
Fragments arranged by size:
4606 1730 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4304 844
Size: 3460 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3096 3277 5444 5618 65
Size: 1183 1848 181 2167 174 1396
Fragments arranged by size:
2167 1848 1396 1183 181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 5593 5780 1700
Size: 110 845 2938 187 2869
Fragments arranged by size:
2938 2869 845 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 2718 5009 6021 2718
Size: 2291 1012 3646
Fragments arranged by size:
3646 2291 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2492 3033 794
Size: 1698 541 4710
Fragments arranged by size:
4710 1698 541
BstBI TT'CG_AA
Cuts at: 2007 5753 2007
Size: 3746 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6949
BstXI CCAn_nnnn'nTGG
Cuts at: 4063 4063
Size: 6949
BstYI r'GATC_y
Cuts at: 1972 2346 3471 3689 4279 4646 5031 5048 6500
Size: 374 1125 218 590 367 385 17 1452
Cuts at: 6500 6586 6598 6944 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1125 590 385 374 367 346
218 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3495 3783 3802 4062 513
Size: 552 2430 288 19 260 3400
Fragments arranged by size:
3400 2430 552 288 260 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4042 487
Size: 683 56 2816 3394
Fragments arranged by size:
3394 2816 683 56
BtsI GCAGTG_nn'
Cuts at: 2333 3507 4465 5259 5279 2333
Size: 1174 958 794 20 4003
Fragments arranged by size:
4003 1174 958 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 4253 4253
Size: 6949
DraIII CAC_nnn'GTG
Cuts at: 3244 3244
Size: 6949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5956 809
Size: 507 4640 1802
Fragments arranged by size:
4640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3168 3207 3616
Size: 54 1425 50 275 18 1338 39 409
Cuts at: 3616 3674 3973 4396 5285 6914 8
Size: 58 299 423 889 1629 43
Fragments arranged by size:
1629 1425 1338 889 423 409 299 275
58 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2475 2720 2843 3604 3780 3885 4879 1353
Size: 1122 245 123 761 176 105 994 3423
Fragments arranged by size:
3423 1122 994 761 245 176 123 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 5536 5910 6056 387
Size: 1064 138 678 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 678 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2890 3026 3094 3887 4056 4143
Size: 393 812 446 136 68 793 169 87
Cuts at: 4143 5012 5609 6396 6664 1239
Size: 869 597 787 268 1524
Fragments arranged by size:
1524 869 812 793 787 597 446 393
268 169 136 87 68
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 3041 2360
Size: 681 6268
EcoO109I rG'GnC_Cy
Cuts at: 2807 2929 3515 3669 3715 3738 3739 3946 4045
Size: 122 586 154 46 23 1 207 99
Cuts at: 4045 2807
Size: 5711
Fragments arranged by size:
5711 586 207 154 122 99 46 23
1
EcoRI G'AATT_C
Cuts at: 2710 4258 4636 2710
Size: 1548 378 5023
Fragments arranged by size:
5023 1548 378
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 2173 2464 2688 3061 3628 6096 1678
Size: 495 291 224 373 567 2468 2531
Fragments arranged by size:
2531 2468 567 495 373 291 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3002 3034 3798 3830 5590
Size: 32 135 32 1430 32 764 32 1760
Cuts at: 5590 5622 5664 5696 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1760 1430 764 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3817 4293 4539 669
Size: 988 281 1879 476 246 3079
Fragments arranged by size:
3079 1879 988 476 281 246
HindIII A'AGCT_T
Cuts at: 748 2794 748
Size: 2046 4903
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KasI G'GCGC_C
Cuts at: 2169 2460 2684 3624 2169
Size: 291 224 940 5494
Fragments arranged by size:
5494 940 291 224
KpnI G_GTAC'C
Cuts at: 2521 4274 2521
Size: 1753 5196
MfeI C'AATT_G
Cuts at: 4548 4548
Size: 6949
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6063 6247 1305
Size: 4758 184 2007
Fragments arranged by size:
4758 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3170 3209 3975 10
Size: 54 1425 343 1338 39 766 2984
Fragments arranged by size:
2984 1425 1338 766 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 3500 4061 4907 5266 5425 518
Size: 648 2334 561 846 359 159 2042
Fragments arranged by size:
2334 2042 846 648 561 359 159
MspA1I CmG'CkG
Cuts at: 1606 2701 3070 3522 3853 3869 3952 4365 5042
Size: 1095 369 452 331 16 83 413 677
Cuts at: 5042 6190 6435 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1095 677 452 413 369 331
245 83 16
NaeI GCC'GGC
Cuts at: 2657 2657
Size: 6949
NarI GG'CG_CC
Cuts at: 2170 2461 2685 3625 2170
Size: 291 224 940 5494
Fragments arranged by size:
5494 940 291 224
NcoI C'CATG_G
Cuts at: 513 1065 3495 3783 3802 4062 513
Size: 552 2430 288 19 260 3400
Fragments arranged by size:
3400 2430 552 288 260 19
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6949
NgoMIV G'CCGG_C
Cuts at: 2655 2655
Size: 6949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 4307 5852 1132
Size: 97 3078 1545 2229
Fragments arranged by size:
3078 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3190 3734 4132 1348
Size: 1842 544 398 4165
Fragments arranged by size:
4165 1842 544 398
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2293 2356 3262 1108
Size: 1185 63 906 4795
Fragments arranged by size:
4795 1185 906 63
PfoI T'CCnGG_A
Cuts at: 2157 2157
Size: 6949
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PmlI CAC'GTG
Cuts at: 2749 3241 2749
Size: 492 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5018 5050 6531 6563 3560
Size: 32 1426 32 1481 32 3946
Fragments arranged by size:
3946 1481 1426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 3715 4045 2929
Size: 786 330 5833
Fragments arranged by size:
5833 786 330
PshAI GACnn'nnGTC
Cuts at: 1613 2357 2958 1613
Size: 744 601 5604
Fragments arranged by size:
5604 744 601
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PspOMI G'GGCC_C
Cuts at: 2466 3515 3738 3946 2466
Size: 1049 223 208 5469
Fragments arranged by size:
5469 1049 223 208
PspXI vC'TCGA_Gb
Cuts at: 2508 2508
Size: 6949
PstI C_TGCA'G
Cuts at: 830 2340 2550 3069 4301 830
Size: 1510 210 519 1232 3478
Fragments arranged by size:
3478 1510 1232 519 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
PvuII CAG'CTG
Cuts at: 2701 3070 3522 3869 2701
Size: 369 452 347 5781
Fragments arranged by size:
5781 452 369 347
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SanDI GG'GwC_CC
Cuts at: 4045 4045
Size: 6949
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 5197 6909 5197
Size: 1712 5237
SfcI C'TryA_G
Cuts at: 826 1045 2336 2546 3065 4297 5432 6113 6304
Size: 219 1291 210 519 1232 1135 681 191
Cuts at: 6304 826
Size: 1471
Fragments arranged by size:
1471 1291 1232 1135 681 519 219 210
191
SfoI GGC'GCC
Cuts at: 2171 2462 2686 3626 2171
Size: 291 224 940 5494
Fragments arranged by size:
5494 940 291 224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 3140 4276 1872
Size: 1268 1136 4545
Fragments arranged by size:
4545 1268 1136
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2508 2797 3685 4026 5053
Size: 197 926 111 454 289 888 341 1027
Cuts at: 5053 5954 6216 820
Size: 901 262 1553
Fragments arranged by size:
1553 1027 926 901 888 454 341 289
262 197 111
SnaBI TAC'GTA
Cuts at: 493 4246 4740 493
Size: 3753 494 2702
Fragments arranged by size:
3753 2702 494
SpeI A'CTAG_T
Cuts at: 152 3427 152
Size: 3275 3674
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 4873 5
Size: 47 4821 2081
Fragments arranged by size:
4821 2081 47
StuI AGG'CCT
Cuts at: 1517 3545 1517
Size: 2028 4921
StyI C'CwwG_G
Cuts at: 513 1065 3325 3370 3495 3629 3783 3802 4062
Size: 552 2260 45 125 134 154 19 260
Cuts at: 4062 4388 513
Size: 326 3074
Fragments arranged by size:
3074 2260 552 326 260 154 134 125
45 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 2762 4976 4993 5146 5331 5776
Size: 1255 759 423 2214 17 153 185 445
Cuts at: 5776 325
Size: 1498
Fragments arranged by size:
2214 1498 1255 759 445 423 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2922 5195 6907
Size: 274 80 33 51 1443 945 2273 1712
Cuts at: 6907 96
Size: 138
Fragments arranged by size:
2273 1712 1443 945 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 3358 3390 3840 3872 6909 6941 2055
Size: 32 1271 32 450 32 3037 32 2063
Fragments arranged by size:
3037 2063 1271 450 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4085 1316
Size: 2769 4180
XbaI T'CTAG_A
Cuts at: 2314 4285 2314
Size: 1971 4978
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3263 3306 3368 3263
Size: 43 62 6844
Fragments arranged by size:
6844 62 43
XhoI C'TCGA_G
Cuts at: 1943 2508 4026 1943
Size: 565 1518 4866
Fragments arranged by size:
4866 1518 565
XmaI C'CCGG_G
Cuts at: 1870 3138 4274 1870
Size: 1268 1136 4545
Fragments arranged by size:
4545 1268 1136
XmnI GAAnn'nnTTC
Cuts at: 3575 5078 3575
Size: 1503 5446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PsrI RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI