Restriction map for
pFN21ASDA0046
(Circular) (Six-base) MAPSORT of: pFN21ASDA0046.seq Check: 7290 from: 1 to: 7336
pFN21ASDA0046 7336 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2049 4533 2049
Size: 2484 4852
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5146 278
Size: 53 83 186 936 3610 2468
Fragments arranged by size:
3610 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3858 4679 1937
Size: 1921 821 4594
Fragments arranged by size:
4594 1921 821
Acc65I G'GTAC_C
Cuts at: 2801 4371 4657 2801
Size: 1570 286 5480
Fragments arranged by size:
5480 1570 286
AclI AA'CG_TT
Cuts at: 1563 5463 5836 1563
Size: 3900 373 3063
Fragments arranged by size:
3900 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5399 6783 7051 1632
Size: 3767 1384 268 1917
Fragments arranged by size:
3767 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7139
AflIII A'CryG_T
Cuts at: 1203 4086 6235 7212 1203
Size: 2883 2149 977 1327
Fragments arranged by size:
2883 2149 1327 977
AgeI A'CCGG_T
Cuts at: 6167 6167
Size: 7336
AhdI GACnn_n'nnGTC
Cuts at: 6065 6065
Size: 7336
AleI CACnn'nnGTG
Cuts at: 1166 3182 1166
Size: 2016 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3381 1184
Size: 2197 5139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 7304
AlwNI CAG_nnn'CTG
Cuts at: 3883 4150 5079 6651 3883
Size: 267 929 1572 4568
Fragments arranged by size:
4568 1572 929 267
ApaLI G'TGCA_C
Cuts at: 3119 5393 6549 3119
Size: 2274 1156 3906
Fragments arranged by size:
3906 2274 1156
ApoI r'AATT_y
Cuts at: 1493 3286 4589 4645 4871 5023 7132 1493
Size: 1793 1303 56 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 1303 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 5890 160
Size: 872 4858 1606
Fragments arranged by size:
4858 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7336
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2501 4020 4661 1870
Size: 73 156 402 1519 641 4545
Fragments arranged by size:
4545 1519 641 402 156 73
BamHI G'GATC_C
Cuts at: 4666 5033 4666
Size: 367 6969
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2533 2788 2801 4371 4657
Size: 325 217 627 746 255 13 1570 286
Cuts at: 4657 6112 618
Size: 1455 1842
Fragments arranged by size:
1842 1570 1455 746 627 325 286 255
217 13
BanII G_rGCy'C
Cuts at: 1391 2607 2850 3687 3843 4655 1391
Size: 1216 243 837 156 812 4072
Fragments arranged by size:
4072 1216 837 812 243 156
BbeI G_GCGC'C
Cuts at: 2792 2792
Size: 7336
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2437 4212 4483 928
Size: 971 538 1775 271 3781
Fragments arranged by size:
3781 1775 971 538 271
BbvCI CC'TCA_GC
Cuts at: 4607 4607
Size: 7336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 5527 5561 1903
Size: 34 2561 34 995 34 3678
Fragments arranged by size:
3678 2561 995 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2490 5228 6438 2490
Size: 2738 1210 3388
Fragments arranged by size:
3388 2738 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7336
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2391 3771 4352
Size: 107 122 71 631 605 719 1380 581
Cuts at: 4352 5947 136
Size: 1595 1525
Fragments arranged by size:
1595 1525 1380 719 631 605 581 122
107 71
BglII A'GATC_T
Cuts at: 7331 7331
Size: 7336
BlpI GC'TnA_GC
Cuts at: 4753 4753
Size: 7336
Bme1580I G_kGCm'C
Cuts at: 1790 3123 5397 6553 1790
Size: 1333 2274 1156 2573
Fragments arranged by size:
2573 2274 1333 1156
BmgBI CAC'GTC
Cuts at: 1318 4480 1318
Size: 3162 4174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5067 6025 442
Size: 416 53 83 399 24 3650 958 1753
Fragments arranged by size:
3650 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 4182 2678
Size: 1504 5832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2619 2787 3251 3359 5996 1239
Size: 1380 168 464 108 2637 2579
Fragments arranged by size:
2637 2579 1380 464 168 108
Bpu10I CC'TnA_GC
Cuts at: 3132 4607 3132
Size: 1475 5861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 5461 6326 6624 2330
Size: 3131 865 298 3042
Fragments arranged by size:
3131 3042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2046 2081 2213 2312 5999 882
Size: 617 547 35 132 99 3687 2219
Fragments arranged by size:
3687 2219 617 547 132 99 35
BsaAI yAC'GTr
Cuts at: 493 1204 2853 3760 4633 5127 493
Size: 711 1649 907 873 494 2702
Fragments arranged by size:
2702 1649 907 873 711 494
BsaBI GATnn'nnATC
Cuts at: 2181 7330 2181
Size: 5149 2187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2789 5143 5525 6160
Size: 53 83 186 936 1256 2354 382 635
Cuts at: 6160 275
Size: 1451
Fragments arranged by size:
2354 1451 1256 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2553 5769 6167 6441 6588 6999 2553
Size: 3216 398 274 147 411 2890
Fragments arranged by size:
3216 2890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2813 2843 4211 4241 4539
Size: 30 165 30 1410 30 1368 30 298
Cuts at: 4539 4569 1178
Size: 30 3945
Fragments arranged by size:
3945 1410 1368 298 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2147 2150 2390 2834 4127 4235 4305
Size: 931 30 3 240 444 1293 108 70
Cuts at: 4305 1186
Size: 4217
Fragments arranged by size:
4217 1293 931 444 240 108 70 30
3
BseYI C'CCAG_C
Cuts at: 2928 6539 7035 2928
Size: 3611 496 3229
Fragments arranged by size:
3611 3229 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 2275 2401 3618 1163
Size: 573 317 222 126 1217 4881
Fragments arranged by size:
4881 1217 573 317 222 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4265 4786 5547 6575 1540
Size: 275 186 2264 521 761 1028 2301
Fragments arranged by size:
2301 2264 1028 761 521 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 2981 3123 4101 5397 5482 6553 2865
Size: 116 142 978 1296 85 1071 3648
Fragments arranged by size:
3648 1296 1071 978 142 116 85
BsmI GAATG_Cn'
Cuts at: 3221 4847 4940 3221
Size: 1626 93 5617
Fragments arranged by size:
5617 1626 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2555 4468 4486 2555
Size: 1913 18 5405
Fragments arranged by size:
5405 1913 18
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2607 2850 2865 2981 3123 3687 3843
Size: 399 817 243 15 116 142 564 156
Cuts at: 3843 4101 4655 5397 5482 6553 1391
Size: 258 554 742 85 1071 2174
Fragments arranged by size:
2174 1071 817 742 564 554 399 258
243 156 142 116 85 15
BspEI T'CCGG_A
Cuts at: 2553 6999 2553
Size: 4446 2890
BspHI T'CATG_A
Cuts at: 3960 5225 6955 3960
Size: 1265 1730 4341
Fragments arranged by size:
4341 1730 1265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2477 4533 4691 844
Size: 1205 428 2056 158 3489
Fragments arranged by size:
3489 2056 1205 428 158
BsrBI CCG'CTC
Cuts at: 1394 1823 2759 4225 5223 1394
Size: 429 936 1466 998 3507
Fragments arranged by size:
3507 1466 998 936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5831 6005 65
Size: 1183 4583 174 1396
Fragments arranged by size:
4583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2476 5980 6167 1700
Size: 110 666 3504 187 2869
Fragments arranged by size:
3504 2869 666 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7336
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7336
BssSI C'ACGA_G
Cuts at: 5396 6408 5396
Size: 1012 6324
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2064 4147 794
Size: 1270 2083 3983
Fragments arranged by size:
3983 2083 1270
BstBI TT'CG_AA
Cuts at: 2007 6140 2007
Size: 4133 3203
BstEII G'GTnAC_C
Cuts at: 1184 3172 1184
Size: 1988 5348
BstXI CCAn_nnnn'nTGG
Cuts at: 2948 3184 3392 2948
Size: 236 208 6892
Fragments arranged by size:
6892 236 208
BstYI r'GATC_y
Cuts at: 1972 2694 4666 5033 5418 5435 6887 6973 6985
Size: 722 1972 367 385 17 1452 86 12
Cuts at: 6985 7331 1972
Size: 346 1977
Fragments arranged by size:
1977 1972 1452 722 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2223 3180 3903 3940 4215 4246
Size: 552 957 201 957 723 37 275 31
Cuts at: 4246 4367 4530 513
Size: 121 163 3319
Fragments arranged by size:
3319 957 957 723 552 275 201 163
121 37 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4087 487
Size: 683 56 2861 3736
Fragments arranged by size:
3736 2861 683 56
BtsI GCAGTG_nn'
Cuts at: 4852 5646 5666 4852
Size: 794 20 6522
Fragments arranged by size:
6522 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7301
DraI TTT'AAA
Cuts at: 4640 4640
Size: 7336
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6343 809
Size: 507 5027 1802
Fragments arranged by size:
5027 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3336 3711 4353
Size: 54 1425 50 275 18 1506 375 642
Cuts at: 4353 4783 5672 7301 8
Size: 430 889 1629 43
Fragments arranged by size:
1629 1506 1425 889 642 430 375 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4783 1537
Size: 275 2971 4090
Fragments arranged by size:
4090 2971 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2612 3238 3350 3386 5266 1353
Size: 986 273 626 112 36 1880 3423
Fragments arranged by size:
3423 1880 986 626 273 112 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4220 5923 6297 6443 387
Size: 1064 138 2631 1703 374 146 1280
Fragments arranged by size:
2631 1703 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2619 2787 3251 3359 5399 5996 6783
Size: 393 987 168 464 108 2040 597 787
Cuts at: 6783 7051 1239
Size: 268 1524
Fragments arranged by size:
2040 1524 987 787 597 464 393 268
168 108
EcoO109I rG'GnC_Cy
Cuts at: 2572 2624 3055 3579 4132 2572
Size: 52 431 524 553 5776
Fragments arranged by size:
5776 553 524 431 52
EcoRI G'AATT_C
Cuts at: 3286 4645 5023 3286
Size: 1359 378 5599
Fragments arranged by size:
5599 1359 378
EcoRV GAT'ATC
Cuts at: 3754 3754
Size: 7336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 7336
FspI TGC'GCA
Cuts at: 3899 5842 3899
Size: 1943 5393
HaeII r_GCGC'y
Cuts at: 1678 2792 4323 6483 1678
Size: 1114 1531 2160 2531
Fragments arranged by size:
2531 2160 1531 1114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 2813 2845 5977
Size: 32 135 32 1160 32 49 32 3132
Cuts at: 5977 6009 6051 6083 1373
Size: 32 42 32 2626
Fragments arranged by size:
3132 2626 1160 135 49 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4680 4926 669
Size: 988 281 2742 246 3079
Fragments arranged by size:
3079 2742 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7336
HpaI GTT'AAC
Cuts at: 4926 4926
Size: 7336
KasI G'GCGC_C
Cuts at: 2788 2788
Size: 7336
KpnI G_GTAC'C
Cuts at: 2805 4375 4661 2805
Size: 1570 286 5480
Fragments arranged by size:
5480 1570 286
MfeI C'AATT_G
Cuts at: 4935 4935
Size: 7336
MluI A'CGCG_T
Cuts at: 7212 7212
Size: 7336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3434 6450 6634 1305
Size: 2129 3016 184 2007
Fragments arranged by size:
3016 2129 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3338 3713 10
Size: 54 1425 343 1506 375 3633
Fragments arranged by size:
3633 1506 1425 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3182 3390 3704 4421 5294 5653 5812
Size: 648 2016 208 314 717 873 359 159
Cuts at: 5812 518
Size: 2042
Fragments arranged by size:
2042 2016 873 717 648 359 314 208
159
MspA1I CmG'CkG
Cuts at: 1606 3602 3942 4369 4752 5429 6577 6822 1606
Size: 1996 340 427 383 677 1148 245 2120
Fragments arranged by size:
2120 1996 1148 677 427 383 340 245
NarI GG'CG_CC
Cuts at: 2789 2789
Size: 7336
NcoI C'CATG_G
Cuts at: 513 1065 2022 2223 4215 513
Size: 552 957 201 1992 3634
Fragments arranged by size:
3634 1992 957 552 201
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7336
NotI GC'GGCC_GC
Cuts at: 4783 4783
Size: 7336
NspI r_CATG'y
Cuts at: 1132 1229 3453 4090 4694 6239 1132
Size: 97 2224 637 604 1545 2229
Fragments arranged by size:
2229 2224 1545 637 604 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7336
PciI A'CATG_T
Cuts at: 4086 6235 4086
Size: 2149 5187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3883 4390 1108
Size: 2775 507 4054
Fragments arranged by size:
4054 2775 507
PfoI T'CCnGG_A
Cuts at: 2492 2492
Size: 7336
PmeI GTTT'AAAC
Cuts at: 4640 4640
Size: 7336
PmlI CAC'GTG
Cuts at: 2853 3760 2853
Size: 907 6429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 4211 4243 5405 5437 6918 6950 3941
Size: 32 238 32 1162 32 1481 32 4327
Fragments arranged by size:
4327 1481 1162 238 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 3579 4132 2572
Size: 1007 553 5776
Fragments arranged by size:
5776 1007 553
PshAI GACnn'nnGTC
Cuts at: 1613 3226 3854 1613
Size: 1613 628 5095
Fragments arranged by size:
5095 1613 628
PsiI TTA'TAA
Cuts at: 4906 4906
Size: 7336
PstI C_TGCA'G
Cuts at: 830 2060 2779 4143 4599 4688 830
Size: 1230 719 1364 456 89 3478
Fragments arranged by size:
3478 1364 1230 719 456 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7336
SacII CC_GC'GG
Cuts at: 3943 4370 3943
Size: 427 6909
SalI G'TCGA_C
Cuts at: 1936 4678 1936
Size: 2742 4594
SapI GCTCTTCn'nnn_
Cuts at: 2612 3386 2612
Size: 774 6562
SbfI CC_TGCA'GG
Cuts at: 4688 4688
Size: 7336
ScaI AGT'ACT
Cuts at: 5584 7296 5584
Size: 1712 5624
SfcI C'TryA_G
Cuts at: 826 1045 2056 2775 4139 4595 4684 5819 6500
Size: 219 1011 719 1364 456 89 1135 681
Cuts at: 6500 6691 826
Size: 191 1471
Fragments arranged by size:
1471 1364 1135 1011 719 681 456 219
191 89
SfoI GGC'GCC
Cuts at: 2790 2790
Size: 7336
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7336
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7336
SmaI CCC'GGG
Cuts at: 1872 4022 4663 1872
Size: 2150 641 4545
Fragments arranged by size:
4545 2150 641
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 5440 6341 6603 820
Size: 197 926 402 3095 901 262 1553
Fragments arranged by size:
3095 1553 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4633 5127 493
Size: 4140 494 2702
Fragments arranged by size:
4140 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7336
SphI G_CATG'C
Cuts at: 1132 4694 1132
Size: 3562 3774
SspI AAT'ATT
Cuts at: 5 52 5260 5
Size: 47 5208 2081
Fragments arranged by size:
5208 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7336
StyI C'CwwG_G
Cuts at: 513 1065 2022 2116 2153 2223 2575 2842 4215
Size: 552 957 94 37 70 352 267 1373
Cuts at: 4215 4775 513
Size: 560 3074
Fragments arranged by size:
3074 1373 957 560 552 352 267 94
70 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 4251 4490 5363 5380 5533 5718
Size: 1255 774 1897 239 873 17 153 185
Cuts at: 5718 6163 325
Size: 445 1498
Fragments arranged by size:
1897 1498 1255 873 774 445 239 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5582 7294 96
Size: 274 80 33 51 1443 3605 1712 138
Fragments arranged by size:
3605 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4075 4107 4539 4571 7296 7328 4075
Size: 32 432 32 2725 32 4083
Fragments arranged by size:
4083 2725 432 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7336
XbaI T'CTAG_A
Cuts at: 2815 4672 2815
Size: 1857 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3832 4206 3832
Size: 374 6962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7336
XmaI C'CCGG_G
Cuts at: 1870 4020 4661 1870
Size: 2150 641 4545
Fragments arranged by size:
4545 2150 641
XmnI GAAnn'nnTTC
Cuts at: 3956 5465 3956
Size: 1509 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5144 276
Size: 53 83 186 936 3610 2468
Fragments arranged by size:
3610 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SexAI SfiI SrfI SwaI