Restriction map for
pFN21ASDA0043
(Circular) (Six-base) MAPSORT of: pFN21ASDA0043.seq Check: 8692 from: 1 to: 6907
pFN21ASDA0043 6907 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2964 2964
Size: 6907
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2783 4717 278
Size: 53 83 186 936 1247 1934 2468
Fragments arranged by size:
2468 1934 1247 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4250 1937
Size: 2313 4594
Acc65I G'GTAC_C
Cuts at: 4228 4228
Size: 6907
AclI AA'CG_TT
Cuts at: 1563 5034 5407 1563
Size: 3471 373 3063
Fragments arranged by size:
3471 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3452 3692 4970 6354 6622 1632
Size: 1820 240 1278 1384 268 1917
Fragments arranged by size:
1917 1820 1384 1278 268 240
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6710
AflIII A'CryG_T
Cuts at: 1203 3240 5806 6783 1203
Size: 2037 2566 977 1327
Fragments arranged by size:
2566 2037 1327 977
AgeI A'CCGG_T
Cuts at: 5738 5738
Size: 6907
AhdI GACnn_n'nnGTC
Cuts at: 5636 5636
Size: 6907
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3711 1184
Size: 2527 4380
AlwNI CAG_nnn'CTG
Cuts at: 4650 6222 4650
Size: 1572 5335
ApaI G_GGCC'C
Cuts at: 2075 4118 2075
Size: 2043 4864
ApaLI G'TGCA_C
Cuts at: 3440 3809 4964 6120 3440
Size: 369 1155 1156 4227
Fragments arranged by size:
4227 1156 1155 369
ApoI r'AATT_y
Cuts at: 1493 2415 4216 4442 4594 6703 1493
Size: 922 1801 226 152 2109 1697
Fragments arranged by size:
2109 1801 1697 922 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5461 160
Size: 872 4429 1606
Fragments arranged by size:
4429 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 3785 3825 4232 1870
Size: 73 125 1717 40 407 4545
Fragments arranged by size:
4545 1717 407 125 73 40
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2607 2640 2607
Size: 33 6874
BamHI G'GATC_C
Cuts at: 2055 4237 4604 2055
Size: 2182 367 4358
Fragments arranged by size:
4358 2182 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2914 3286 4228 5683 618
Size: 325 217 627 1127 372 942 1455 1842
Fragments arranged by size:
1842 1455 1127 942 627 372 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 2512 3279 3362 3834 4118 4226 1391
Size: 684 437 767 83 472 284 108 4072
Fragments arranged by size:
4072 767 684 472 437 284 108 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 928
Size: 971 272 5664
Fragments arranged by size:
5664 971 272
BbvCI CC'TCA_GC
Cuts at: 3095 3095
Size: 6907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3969 4003 5098 5132 1903
Size: 34 2032 34 1095 34 3678
Fragments arranged by size:
3678 2032 1095 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4799 6009 4799
Size: 1210 5697
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6907
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3308 3590 3608
Size: 107 122 71 631 605 1636 282 18
Cuts at: 3608 3638 5518 136
Size: 30 1880 1525
Fragments arranged by size:
1880 1636 1525 631 605 282 122 107
71 30 18
BglII A'GATC_T
Cuts at: 6902 6902
Size: 6907
BlpI GC'TnA_GC
Cuts at: 3612 3761 4058 4099 4127 4324 3612
Size: 149 297 41 28 197 6195
Fragments arranged by size:
6195 297 197 149 41 28
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2583 2919 3444 3813 4118 4968 6124
Size: 285 508 336 525 369 305 850 1156
Cuts at: 6124 1790
Size: 2573
Fragments arranged by size:
2573 1156 850 525 508 369 336 305
285
BmgBI CAC'GTC
Cuts at: 1318 2649 3241 1318
Size: 1331 592 4984
Fragments arranged by size:
4984 1331 592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2651 3467 4638
Size: 416 53 83 399 24 1234 816 1171
Cuts at: 4638 5596 442
Size: 958 1753
Fragments arranged by size:
1753 1234 1171 958 816 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4004 1056
Size: 2948 3959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 6907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2921 3071 3218 3929 4150 5567 1239
Size: 1682 150 147 711 221 1417 2579
Fragments arranged by size:
2579 1682 1417 711 221 150 147
Bpu10I CC'TnA_GC
Cuts at: 3095 3839 4005 3095
Size: 744 166 5997
Fragments arranged by size:
5997 744 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5032 5897 6195 5032
Size: 865 298 5744
Fragments arranged by size:
5744 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2104 5570 882
Size: 617 605 3466 2219
Fragments arranged by size:
3466 2219 617 605
BsaAI yAC'GTr
Cuts at: 493 1204 4204 4698 493
Size: 711 3000 494 2702
Fragments arranged by size:
3000 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6901 6901
Size: 6907
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2600 2780 4714 5096
Size: 53 83 186 936 1067 180 1934 382
Cuts at: 5096 5731 275
Size: 635 1451
Fragments arranged by size:
1934 1451 1067 936 635 382 186 180
83 53
BsaWI w'CCGG_w
Cuts at: 5340 5738 6012 6159 6570 5340
Size: 398 274 147 411 5677
Fragments arranged by size:
5677 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2755 2785 1178
Size: 30 165 30 1352 30 5300
Fragments arranged by size:
5300 1352 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2673 3368 3404 3407 3734 3737 4010 4132
Size: 1487 695 36 3 327 3 273 122
Cuts at: 4132 1186
Size: 3961
Fragments arranged by size:
3961 1487 695 327 273 122 36 3
3
BseYI C'CCAG_C
Cuts at: 2108 2557 2665 2919 3796 6110 6606 2108
Size: 449 108 254 877 2314 496 2409
Fragments arranged by size:
2409 2314 877 496 449 254 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3041 1163
Size: 573 1305 5029
Fragments arranged by size:
5029 1305 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 2541 3660 4357 5118 6146
Size: 275 186 121 419 1119 697 761 1028
Cuts at: 6146 1540
Size: 2301
Fragments arranged by size:
2301 1119 1028 761 697 419 275 186
121
BsiHKAI G_wGCw'C
Cuts at: 2955 3362 3444 3813 4968 5053 6124 2955
Size: 407 82 369 1155 85 1071 3738
Fragments arranged by size:
3738 1155 1071 407 369 85 82
BsmI GAATG_Cn'
Cuts at: 4418 4511 4418
Size: 93 6814
BsmBI CGTCTCn'nnnn_
Cuts at: 2596 2596
Size: 6907
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2512 2583 2919 2955 3279 3362
Size: 399 285 437 71 336 36 324 83
Cuts at: 3362 3444 3813 3834 4118 4226 4968 5053 6124
Size: 82 369 21 284 108 742 85 1071
Cuts at: 6124 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 437 399 369 336 324
285 284 108 85 83 82 71 36
21
BspEI T'CCGG_A
Cuts at: 6570 6570
Size: 6907
BspHI T'CATG_A
Cuts at: 4796 6526 4796
Size: 1730 5177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2510 2964 4262 844
Size: 1394 272 454 1298 3489
Fragments arranged by size:
3489 1394 1298 454 272
BsrBI CCG'CTC
Cuts at: 1394 1823 3397 4794 1394
Size: 429 1574 1397 3507
Fragments arranged by size:
3507 1574 1397 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5402 5576 65
Size: 1183 4154 174 1396
Fragments arranged by size:
4154 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2497 3289 3659 3777 5551 5738 1700
Size: 110 687 792 370 118 1774 187 2869
Fragments arranged by size:
2869 1774 792 687 370 187 118 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6907
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6907
BssSI C'ACGA_G
Cuts at: 3206 3443 4967 5979 3206
Size: 237 1524 1012 4134
Fragments arranged by size:
4134 1524 1012 237
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 794
Size: 1352 5555
BstBI TT'CG_AA
Cuts at: 2007 5711 2007
Size: 3704 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6907
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 3409 2848
Size: 561 6346
BstYI r'GATC_y
Cuts at: 1972 2055 4237 4604 4989 5006 6458 6544 6556
Size: 83 2182 367 385 17 1452 86 12
Cuts at: 6556 6902 1972
Size: 346 1977
Fragments arranged by size:
2182 1977 1452 385 367 346 86 83
17 12
Bsu36I CC'TnA_GG
Cuts at: 2978 4108 2978
Size: 1130 5777
BtgI C'CryG_G
Cuts at: 513 1065 2049 2208 2706 3219 3279 3306 3315
Size: 552 984 159 498 513 60 27 9
Cuts at: 3315 3654 513
Size: 339 3766
Fragments arranged by size:
3766 984 552 513 498 339 159 60
27 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2593 487
Size: 683 56 1367 4801
Fragments arranged by size:
4801 1367 683 56
BtsI GCAGTG_nn'
Cuts at: 2634 2790 3355 4195 4423 5217 5237 2634
Size: 156 565 840 228 794 20 4304
Fragments arranged by size:
4304 840 794 565 228 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6872
DraI TTT'AAA
Cuts at: 4211 4211
Size: 6907
DraIII CAC_nnn'GTG
Cuts at: 3559 3559
Size: 6907
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5914 809
Size: 507 4598 1802
Fragments arranged by size:
4598 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2538 2784
Size: 54 1425 50 275 18 289 419 246
Cuts at: 2784 2869 3121 3216 3453 3657 4354 5243 6872
Size: 85 252 95 237 204 697 889 1629
Cuts at: 6872 8
Size: 43
Fragments arranged by size:
1629 1425 889 697 419 289 275 252
246 237 204 95 85 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 2538 3657 4354 1537
Size: 275 307 419 1119 697 4090
Fragments arranged by size:
4090 1119 697 419 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 3367 3567 3711 4837 1353
Size: 926 1088 200 144 1126 3423
Fragments arranged by size:
3423 1126 1088 926 200 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3428 3585 5494 5868 6014 387
Size: 1064 138 1839 157 1909 374 146 1280
Fragments arranged by size:
1909 1839 1280 1064 374 157 146 138
EcoICRI GAG'CTC
Cuts at: 3360 3360
Size: 6907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2921 3071 3218 3452 3692 3929 4150
Size: 393 1289 150 147 234 240 237 221
Cuts at: 4150 4970 5567 6354 6622 1239
Size: 820 597 787 268 1524
Fragments arranged by size:
1524 1289 820 787 597 393 268 240
237 234 221 150 147
EcoNI CCTnn'n_nnAGG
Cuts at: 3464 3838 3464
Size: 374 6533
EcoO109I rG'GnC_Cy
Cuts at: 2072 2896 3046 3469 3727 4114 2072
Size: 824 150 423 258 387 4865
Fragments arranged by size:
4865 824 423 387 258 150
EcoRI G'AATT_C
Cuts at: 4216 4594 4216
Size: 378 6529
FspI TGC'GCA
Cuts at: 5413 5413
Size: 6907
HaeII r_GCGC'y
Cuts at: 1678 3296 3333 3757 6054 1678
Size: 1618 37 424 2297 2531
Fragments arranged by size:
2531 2297 1618 424 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2975 3007 5548 5580 5622
Size: 32 135 32 1403 32 2541 32 42
Cuts at: 5622 5654 1373
Size: 32 2626
Fragments arranged by size:
2626 2541 1403 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4251 4497 669
Size: 988 281 2313 246 3079
Fragments arranged by size:
3079 2313 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 748
Size: 1655 5252
HpaI GTT'AAC
Cuts at: 4497 4497
Size: 6907
KpnI G_GTAC'C
Cuts at: 4232 4232
Size: 6907
MfeI C'AATT_G
Cuts at: 4506 4506
Size: 6907
MluI A'CGCG_T
Cuts at: 6783 6783
Size: 6907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2471 2928 3873 6021 6205 1305
Size: 822 344 457 945 2148 184 2007
Fragments arranged by size:
2148 2007 945 822 457 344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3218 10
Size: 54 1425 343 1386 3699
Fragments arranged by size:
3699 1425 1386 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4865 5224 5383 518
Size: 648 3699 359 159 2042
Fragments arranged by size:
3699 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2137 2669 3308 3430 3451 3670 4050
Size: 445 86 532 639 122 21 219 380
Cuts at: 4050 4057 4170 4323 5000 6148 6393 1606
Size: 7 113 153 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 532 445 380 245
219 153 122 113 86 21 7
NaeI GCC'GGC
Cuts at: 2499 3661 2499
Size: 1162 5745
NcoI C'CATG_G
Cuts at: 513 1065 3219 3315 513
Size: 552 2154 96 4105
Fragments arranged by size:
4105 2154 552 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6907
NgoMIV G'CCGG_C
Cuts at: 2497 3659 2497
Size: 1162 5745
NheI G'CTAG_C
Cuts at: 1052 4000 1052
Size: 2948 3959
NotI GC'GGCC_GC
Cuts at: 4354 4354
Size: 6907
NspI r_CATG'y
Cuts at: 1132 1229 2149 4265 5810 1132
Size: 97 920 2116 1545 2229
Fragments arranged by size:
2229 2116 1545 920 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6907
PciI A'CATG_T
Cuts at: 5806 5806
Size: 6907
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 1108
Size: 1101 5806
PfoI T'CCnGG_A
Cuts at: 3234 3234
Size: 6907
PmeI GTTT'AAAC
Cuts at: 4211 4211
Size: 6907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4976 5008 6489 6521 4976
Size: 32 1481 32 5362
Fragments arranged by size:
5362 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2896 2896
Size: 6907
PshAI GACnn'nnGTC
Cuts at: 1613 2603 1613
Size: 990 5917
PsiI TTA'TAA
Cuts at: 2367 4477 2367
Size: 2110 4797
PspOMI G'GGCC_C
Cuts at: 2071 4114 2071
Size: 2043 4864
PstI C_TGCA'G
Cuts at: 830 2142 3423 4041 4259 830
Size: 1312 1281 618 218 3478
Fragments arranged by size:
3478 1312 1281 618 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6907
PvuII CAG'CTG
Cuts at: 2137 2669 3430 3451 3670 4057 2137
Size: 532 761 21 219 387 4987
Fragments arranged by size:
4987 761 532 387 219 21
SacI G_AGCT'C
Cuts at: 3362 3362
Size: 6907
SacII CC_GC'GG
Cuts at: 2052 3309 2052
Size: 1257 5650
SalI G'TCGA_C
Cuts at: 1936 4249 1936
Size: 2313 4594
SapI GCTCTTCn'nnn_
Cuts at: 3367 3367
Size: 6907
SbfI CC_TGCA'GG
Cuts at: 4259 4259
Size: 6907
ScaI AGT'ACT
Cuts at: 5155 6867 5155
Size: 1712 5195
SfcI C'TryA_G
Cuts at: 826 1045 2138 3419 4037 4255 5390 6071 6262
Size: 219 1093 1281 618 218 1135 681 191
Cuts at: 6262 826
Size: 1471
Fragments arranged by size:
1471 1281 1135 1093 681 618 219 218
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6907
SgrAI Cr'CCGG_yG
Cuts at: 1700 3289 1700
Size: 1589 5318
SmaI CCC'GGG
Cuts at: 1872 2070 3787 4234 1872
Size: 198 1717 447 4545
Fragments arranged by size:
4545 1717 447 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5011 5912 6174 820
Size: 197 926 3068 901 262 1553
Fragments arranged by size:
3068 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4204 4698 493
Size: 3711 494 2702
Fragments arranged by size:
3711 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6907
SphI G_CATG'C
Cuts at: 1132 4265 1132
Size: 3133 3774
SspI AAT'ATT
Cuts at: 5 52 4831 5
Size: 47 4779 2081
Fragments arranged by size:
4779 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6907
StyI C'CwwG_G
Cuts at: 513 1065 3219 3315 3585 4346 513
Size: 552 2154 96 270 761 3074
Fragments arranged by size:
3074 2154 761 552 270 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2641 2710 4934 4951 5104 5289 5734
Size: 1255 1061 69 2224 17 153 185 445
Cuts at: 5734 325
Size: 1498
Fragments arranged by size:
2224 1498 1255 1061 445 185 153 69
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 5153 6865
Size: 274 80 33 51 1443 165 3011 1712
Cuts at: 6865 96
Size: 138
Fragments arranged by size:
3011 1712 1443 274 165 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2685 2717 2755 2787 6867 6899 2685
Size: 32 38 32 4080 32 2693
Fragments arranged by size:
4080 2693 38 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6907
XbaI T'CTAG_A
Cuts at: 4243 4243
Size: 6907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2704 2896 2704
Size: 192 6715
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6907
XmaI C'CCGG_G
Cuts at: 1870 2068 3785 4232 1870
Size: 198 1717 447 4545
Fragments arranged by size:
4545 1717 447 198
XmnI GAAnn'nnTTC
Cuts at: 3953 5036 3953
Size: 1083 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2781 4715 276
Size: 53 83 186 936 1247 1934 2468
Fragments arranged by size:
2468 1934 1247 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI