Restriction map for
pFN21ASDA0039
(Circular) (Six-base) MAPSORT of: pFN21ASDA0039.seq Check: 677 from: 1 to: 6127
pFN21ASDA0039 6127 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2569 2569
Size: 6127
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3234 3470 1937
Size: 1297 236 4594
Fragments arranged by size:
4594 1297 236
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4190 5574 5842 1632
Size: 2558 1384 268 1917
Fragments arranged by size:
2558 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2505 3175 5026 6003 1203
Size: 1302 670 1851 977 1327
Fragments arranged by size:
1851 1327 1302 977 670
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 2143 2828 2891 3870 5442 2143
Size: 685 63 979 1572 2828
Fragments arranged by size:
2828 1572 979 685 63
ApaI G_GGCC'C
Cuts at: 3350 3350
Size: 6127
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2176 2414 2759 3436 3662 3814 5923 1493
Size: 683 238 345 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 677 345 238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 1870
Size: 73 1509 4545
Fragments arranged by size:
4545 1509 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2704 3448 4903 618
Size: 325 217 627 917 744 1455 1842
Fragments arranged by size:
1842 1455 917 744 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 2455 3146 3350 3446 1391
Size: 797 267 691 204 96 4072
Fragments arranged by size:
4072 797 691 267 204 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 3287 928
Size: 971 567 821 3768
Fragments arranged by size:
3768 971 821 567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BfrBI ATG'CAT
Cuts at: 2684 2684
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 2099 3544 2099
Size: 1445 4682
Bme1580I G_kGCm'C
Cuts at: 1790 3350 4188 5344 1790
Size: 1560 838 1156 2573
Fragments arranged by size:
2573 1560 1156 838
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2300 1056
Size: 1244 4883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4787 1239
Size: 3548 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3130 4252 5117 5415 3130
Size: 1122 865 298 3842
Fragments arranged by size:
3842 1122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3424 3918 493
Size: 711 2220 494 2702
Fragments arranged by size:
2702 2220 711 494
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5902
Fragments arranged by size:
5902 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2998 3069 3072 3281 1186
Size: 1812 71 3 209 4032
Fragments arranged by size:
4032 1812 209 71 3
BseYI C'CCAG_C
Cuts at: 2580 5330 5826 2580
Size: 2750 496 2881
Fragments arranged by size:
2881 2750 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2454 3235 1163
Size: 573 718 781 4055
Fragments arranged by size:
4055 781 718 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2455 3146 4188 4273 5344 2455
Size: 691 1042 85 1071 3238
Fragments arranged by size:
3238 1071 1042 691 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
BsmBI CGTCTCn'nnnn_
Cuts at: 3093 3093
Size: 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2455 3146 3350 3446 4188 4273
Size: 399 398 267 691 204 96 742 85
Cuts at: 4273 5344 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 398 267 204
96 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 2569 3482 844
Size: 1719 6 913 3489
Fragments arranged by size:
3489 1719 913 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2322 96
Size: 2226 3901
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2855 794
Size: 2061 4066
BstBI TT'CG_AA
Cuts at: 2007 4931 2007
Size: 2924 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122
Size: 1485 367 385 17 1452 86 12 346
Cuts at: 6122 1972
Size: 1977
Fragments arranged by size:
1977 1485 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4618
Fragments arranged by size:
4618 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 2796 3022 3643 4437 4457 2289
Size: 507 226 621 794 20 3959
Fragments arranged by size:
3959 794 621 507 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 2764 3431 2764
Size: 667 5460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2060 5134 809
Size: 507 744 3074 1802
Fragments arranged by size:
3074 1802 744 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092
Size: 54 1425 50 275 18 1744 889 1629
Cuts at: 6092 8
Size: 43
Fragments arranged by size:
1744 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2473 3258 4057 1353
Size: 1120 785 799 3423
Fragments arranged by size:
3423 1120 799 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4714 5088 5234 387
Size: 1064 138 453 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 2453 3144 2453
Size: 691 5436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4190 4787 5574 5842 1239
Size: 393 2558 597 787 268 1524
Fragments arranged by size:
2558 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3346 3347 3346
Size: 1 6126
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3287 3287
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 5274 1678
Size: 3596 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3053 3085 3230 3262 3352
Size: 32 135 32 1481 32 145 32 90
Cuts at: 3352 3384 4768 4800 4842 4874 1373
Size: 32 1384 32 42 32 2626
Fragments arranged by size:
2626 1481 1384 145 135 90 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 3471 3717 669
Size: 988 281 187 1346 246 3079
Fragments arranged by size:
3079 1346 988 281 246 187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 2125 3717 2125
Size: 1592 4535
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 3175 6003 3175
Size: 2828 3299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2092 2318 3314 4085 4444 4603 518
Size: 648 926 226 996 771 359 159 2042
Fragments arranged by size:
2042 996 926 771 648 359 226 159
MspA1I CmG'CkG
Cuts at: 1606 2438 3543 4220 5368 5613 1606
Size: 832 1105 677 1148 245 2120
Fragments arranged by size:
2120 1148 1105 832 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4618
Fragments arranged by size:
4618 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 2296 1052
Size: 1244 4883
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2686 2686
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2198 2509 3485 5030 1132
Size: 97 969 311 976 1545 2229
Fragments arranged by size:
2229 1545 976 969 311 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 2505 5026 2505
Size: 2521 3606
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3391 1613
Size: 1778 4349
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 3346 3346
Size: 6127
PstI C_TGCA'G
Cuts at: 830 2411 2692 2860 2907 3479 830
Size: 1581 281 168 47 572 3478
Fragments arranged by size:
3478 1581 572 281 168 47
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2438 2438
Size: 6127
SacI G_AGCT'C
Cuts at: 2455 3146 2455
Size: 691 5436
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 2860 3479 2860
Size: 619 5508
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2407 2688 2820 2856 2903 3475 4610
Size: 219 1362 281 132 36 47 572 1135
Cuts at: 4610 5291 5482 826
Size: 681 191 1471
Fragments arranged by size:
1471 1362 1135 681 572 281 219 191
132 47 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3145 4231 5132 5394 820
Size: 197 926 1202 1086 901 262 1553
Fragments arranged by size:
1553 1202 1086 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3424 3918 493
Size: 2931 494 2702
Fragments arranged by size:
2931 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 2862 1517
Size: 1345 4782
StyI C'CwwG_G
Cuts at: 513 1065 2022 3566 513
Size: 552 957 1544 3074
Fragments arranged by size:
3074 1544 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4154 4171 4324 4509 4954 325
Size: 1255 2574 17 153 185 445 1498
Fragments arranged by size:
2574 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 3292 4373
Size: 274 80 33 51 1443 345 970 1081
Cuts at: 4373 6085 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1081 970 345 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2596
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 3030 4256 3030
Size: 1226 4901
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI