Restriction map for
pFN21ASDA0036
(Circular) (Six-base) MAPSORT of: pFN21ASDA0036.seq Check: 7185 from: 1 to: 6523
pFN21ASDA0036 6523 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2800 4333 278
Size: 53 83 186 936 1264 1533 2468
Fragments arranged by size:
2468 1533 1264 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2800 3866 1937
Size: 863 1066 4594
Fragments arranged by size:
4594 1066 863
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 3573 4650 5023 1563
Size: 2010 1077 373 3063
Fragments arranged by size:
3063 2010 1077 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2439 3416 3623 3713 4586 5970 6238
Size: 456 351 977 207 90 873 1384 268
Cuts at: 6238 1632
Size: 1917
Fragments arranged by size:
1917 1384 977 873 456 351 268 207
90
AfeI AGC'GCT
Cuts at: 3303 3303
Size: 6523
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 2393 5422 6399 1203
Size: 1190 3029 977 1327
Fragments arranged by size:
3029 1327 1190 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 3712 1166
Size: 2546 3977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 4266 5838 4266
Size: 1572 4951
ApaI G_GGCC'C
Cuts at: 3517 3517
Size: 6523
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 2327 2362 2751 2828 3238 3308 3787 3832
Size: 834 35 389 77 410 70 479 45
Cuts at: 3832 4058 4210 6319 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 479 410 389 226 152
77 70 45 35
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 3111 3848 1870
Size: 73 1168 737 4545
Fragments arranged by size:
4545 1168 737 73
BamHI G'GATC_C
Cuts at: 3853 4220 3853
Size: 367 6156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3592 3844 5299 618
Size: 325 217 627 1805 252 1455 1842
Fragments arranged by size:
1842 1805 1455 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 3437 3468 3517 3532 3633 3842 1391
Size: 2046 31 49 15 101 209 4072
Fragments arranged by size:
4072 2046 209 101 49 31 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3430 928
Size: 971 1531 4021
Fragments arranged by size:
4021 1531 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4714 4748 1903
Size: 34 2777 34 3678
Fragments arranged by size:
3678 2777 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4415 5625 4415
Size: 1210 5313
BclI T'GATC_A
Cuts at: 1548 2521 1548
Size: 973 5550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3674 5134 136
Size: 107 122 71 631 605 2002 1460 1525
Fragments arranged by size:
2002 1525 1460 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6518 6518
Size: 6523
BlpI GC'TnA_GC
Cuts at: 3940 3940
Size: 6523
Bme1580I G_kGCm'C
Cuts at: 1790 3517 4584 5740 1790
Size: 1727 1067 1156 2573
Fragments arranged by size:
2573 1727 1156 1067
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6523
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 4254 5212
Size: 416 53 83 399 24 1412 1425 958
Cuts at: 5212 442
Size: 1753
Fragments arranged by size:
1753 1425 1412 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3276 3276
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3053 3759 5183 1239
Size: 1814 706 1424 2579
Fragments arranged by size:
2579 1814 1424 706
Bpu10I CC'TnA_GC
Cuts at: 2085 2412 3627 2085
Size: 327 1215 4981
Fragments arranged by size:
4981 1215 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3465 4648 5513 5811 3465
Size: 1183 865 298 4177
Fragments arranged by size:
4177 1183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5186 882
Size: 617 3687 2219
Fragments arranged by size:
3687 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3820 4314 493
Size: 711 2616 494 2702
Fragments arranged by size:
2702 2616 711 494
BsaBI GATnn'nnATC
Cuts at: 6517 6517
Size: 6523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2797 4330 4712 5347
Size: 53 83 186 936 1264 1533 382 635
Cuts at: 5347 275
Size: 1451
Fragments arranged by size:
1533 1451 1264 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4956 5354 5628 5775 6186 4956
Size: 398 274 147 411 5293
Fragments arranged by size:
5293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2505 1178
Size: 30 165 30 1072 30 5196
Fragments arranged by size:
5196 1072 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3013 1186
Size: 1827 4696
BseYI C'CCAG_C
Cuts at: 2265 3531 5726 6222 2265
Size: 1266 2195 496 2566
Fragments arranged by size:
2566 2195 1266 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5950
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3973 4734 5762 1540
Size: 275 186 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3468 3633 4584 4669 5740 3468
Size: 165 951 85 1071 4251
Fragments arranged by size:
4251 1071 951 165 85
BsmI GAATG_Cn'
Cuts at: 4034 4127 4034
Size: 93 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3437 3468 3517 3532 3633 3842 4584
Size: 399 1647 31 49 15 101 209 742
Cuts at: 4584 4669 5740 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1647 1071 742 399 209 101 85
49 31 15
BspEI T'CCGG_A
Cuts at: 6186 6186
Size: 6523
BspHI T'CATG_A
Cuts at: 2574 4412 6142 2574
Size: 1838 1730 2955
Fragments arranged by size:
2955 1838 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 3878 844
Size: 1942 1092 3489
Fragments arranged by size:
3489 1942 1092
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2287 5018 5192 65
Size: 1183 1039 2731 174 1396
Fragments arranged by size:
2731 1396 1183 1039 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5167 5354 1700
Size: 110 3357 187 2869
Fragments arranged by size:
3357 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 2007 5327 2007
Size: 3320 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstXI CCAn_nnnn'nTGG
Cuts at: 3655 3655
Size: 6523
BstYI r'GATC_y
Cuts at: 1972 2049 2564 3008 3644 3853 4220 4605 4622
Size: 77 515 444 636 209 367 385 17
Cuts at: 4622 6074 6160 6172 6518 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 636 515 444 385 367 346
209 86 77 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5971
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5784
Fragments arranged by size:
5784 683 56
BtsI GCAGTG_nn'
Cuts at: 4039 4833 4853 4039
Size: 794 20 5709
Fragments arranged by size:
5709 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2796 5530 809
Size: 507 1480 2734 1802
Fragments arranged by size:
2734 1802 1480 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3970 4859 6488
Size: 54 1425 50 275 18 2140 889 1629
Cuts at: 6488 8
Size: 43
Fragments arranged by size:
2140 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2474 2886 3051 3177 3516 3638 4453 1353
Size: 1121 412 165 126 339 122 815 3423
Fragments arranged by size:
3423 1121 815 412 339 165 126 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3466 3631 3466
Size: 165 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2439 3053 3416 3623 3713 3759
Size: 393 456 351 614 363 207 90 46
Cuts at: 3759 4586 5183 5970 6238 1239
Size: 827 597 787 268 1524
Fragments arranged by size:
1524 827 787 614 597 456 393 363
351 268 207 90 46
EcoNI CCTnn'n_nnAGG
Cuts at: 3354 3354
Size: 6523
EcoO109I rG'GnC_Cy
Cuts at: 3061 3513 3514 3061
Size: 452 1 6070
Fragments arranged by size:
6070 452 1
EcoRI G'AATT_C
Cuts at: 2828 3832 4210 2828
Size: 1004 378 5141
Fragments arranged by size:
5141 1004 378
FspI TGC'GCA
Cuts at: 5029 5029
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 3305 5670 1678
Size: 1627 2365 2531
Fragments arranged by size:
2531 2365 1627
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2449 2481 2556 2588 5164
Size: 32 135 32 877 32 75 32 2576
Cuts at: 5164 5196 5238 5270 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2576 877 135 75 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3867 4113 669
Size: 988 281 1929 246 3079
Fragments arranged by size:
3079 1929 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2672 2969 3722 748
Size: 1924 297 753 3549
Fragments arranged by size:
3549 1924 753 297
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 3074 4122 3074
Size: 1048 5475
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2231 5637 5821 1305
Size: 926 3406 184 2007
Fragments arranged by size:
3406 2007 926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4701
Fragments arranged by size:
4701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 2579 3712 4481 4840 4999 518
Size: 648 1126 287 1133 769 359 159 2042
Fragments arranged by size:
2042 1133 1126 769 648 359 287 159
MspA1I CmG'CkG
Cuts at: 1606 2354 3124 3281 3939 4616 5764 6009 1606
Size: 748 770 157 658 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 748 677 658 245 157
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5971
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 2397 2919 3881 5426 1132
Size: 97 1168 522 962 1545 2229
Fragments arranged by size:
2229 1545 1168 962 522 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6523
PciI A'CATG_T
Cuts at: 2393 5422 2393
Size: 3029 3494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6523
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3615 3647 4592 4624 6105 6137 3615
Size: 32 945 32 1481 32 4001
Fragments arranged by size:
4001 1481 945 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3061 3061
Size: 6523
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6523
PsiI TTA'TAA
Cuts at: 2699 4093 2699
Size: 1394 5129
PspOMI G'GGCC_C
Cuts at: 3513 3513
Size: 6523
PstI C_TGCA'G
Cuts at: 830 2359 2572 3875 830
Size: 1529 213 1303 3478
Fragments arranged by size:
3478 1529 1303 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2354 3124 3281 2354
Size: 770 157 5596
Fragments arranged by size:
5596 770 157
SacI G_AGCT'C
Cuts at: 3468 3633 3468
Size: 165 6358
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SapI GCTCTTCn'nnn_
Cuts at: 2886 3516 3638 2886
Size: 630 122 5771
Fragments arranged by size:
5771 630 122
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 2707 4771 6483 2707
Size: 2064 1712 2747
Fragments arranged by size:
2747 2064 1712
SfcI C'TryA_G
Cuts at: 826 1045 2058 2355 2568 2585 3871 5006 5687
Size: 219 1013 297 213 17 1286 1135 681
Cuts at: 5687 5878 826
Size: 191 1471
Fragments arranged by size:
1471 1286 1135 1013 681 297 219 213
191 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 3674 3674
Size: 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3850 1872
Size: 1978 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3480 4627 5528 5790 820
Size: 197 926 1537 1147 901 262 1553
Fragments arranged by size:
1553 1537 1147 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3820 4314 493
Size: 3327 494 2702
Fragments arranged by size:
3327 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 2919 3881 1132
Size: 1787 962 3774
Fragments arranged by size:
3774 1787 962
SspI AAT'ATT
Cuts at: 5 52 2204 4447 5
Size: 47 2152 2243 2081
Fragments arranged by size:
2243 2152 2081 47
StuI AGG'CCT
Cuts at: 1517 3652 1517
Size: 2135 4388
StyI C'CwwG_G
Cuts at: 513 1065 2045 3350 3663 3962 513
Size: 552 980 1305 313 299 3074
Fragments arranged by size:
3074 1305 980 552 313 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4550 4567 4720 4905 5350 325
Size: 1255 2970 17 153 185 445 1498
Fragments arranged by size:
2970 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2509 2705 4769
Size: 274 80 33 51 1443 532 196 2064
Cuts at: 4769 6481 96
Size: 1712 138
Fragments arranged by size:
2064 1712 1443 532 274 196 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3186 3218 6483 6515 3186
Size: 32 3265 32 3194
Fragments arranged by size:
3265 3194 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2552 3416 1316
Size: 1236 864 4423
Fragments arranged by size:
4423 1236 864
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3848 1870
Size: 1978 4545
XmnI GAAnn'nnTTC
Cuts at: 2343 2364 2499 3141 4652 2343
Size: 21 135 642 1511 4214
Fragments arranged by size:
4214 1511 642 135 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2798 4331 276
Size: 53 83 186 936 1264 1533 2468
Fragments arranged by size:
2468 1533 1264 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfoI SrfI SwaI XcmI