Restriction map for
pFN21ASDA0035
(Circular) (Six-base) MAPSORT of: pFN21ASDA0035.seq Check: 1337 from: 1 to: 6856
pFN21ASDA0035 6856 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3253 3253
Size: 6856
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4666 278
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4199 1937
Size: 2262 4594
Acc65I G'GTAC_C
Cuts at: 3197 4177 3197
Size: 980 5876
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3072 3123 3851 4919 6303 6571 1632
Size: 1440 51 728 1068 1384 268 1917
Fragments arranged by size:
1917 1440 1384 1068 728 268 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 5755 6732 1203
Size: 4552 977 1327
Fragments arranged by size:
4552 1327 977
AgeI A'CCGG_T
Cuts at: 5687 5687
Size: 6856
AhdI GACnn_n'nnGTC
Cuts at: 5585 5585
Size: 6856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6856
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2959 2991 2959
Size: 32 6824
AlwNI CAG_nnn'CTG
Cuts at: 2777 3155 3575 4599 6171 2777
Size: 378 420 1024 1572 3462
Fragments arranged by size:
3462 1572 1024 420 378
ApaLI G'TGCA_C
Cuts at: 4913 6069 4913
Size: 1156 5700
ApoI r'AATT_y
Cuts at: 1493 3208 4165 4391 4543 6652 1493
Size: 1715 957 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 957 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 4181 1870
Size: 73 688 1550 4545
Fragments arranged by size:
4545 1550 688 73
BamHI G'GATC_C
Cuts at: 4186 4553 4186
Size: 367 6489
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3197 3695 4177 5632 618
Size: 325 217 627 1410 498 482 1455 1842
Fragments arranged by size:
1842 1455 1410 627 498 482 325 217
BanII G_rGCy'C
Cuts at: 1391 2522 3137 3665 4175 1391
Size: 1131 615 528 510 4072
Fragments arranged by size:
4072 1131 615 528 510
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3027 3368 3509 928
Size: 971 1128 341 141 4275
Fragments arranged by size:
4275 1128 971 341 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5047 5081 1903
Size: 34 3110 34 3678
Fragments arranged by size:
3678 3110 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2076 4748 5958 2076
Size: 2672 1210 2974
Fragments arranged by size:
2974 2672 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3434 3686 3833
Size: 107 122 71 631 605 1762 252 147
Cuts at: 3833 5467 136
Size: 1634 1525
Fragments arranged by size:
1762 1634 1525 631 605 252 147 122
107 71
BglII A'GATC_T
Cuts at: 6851 6851
Size: 6856
BlpI GC'TnA_GC
Cuts at: 4273 4273
Size: 6856
Bme1580I G_kGCm'C
Cuts at: 1790 4917 6073 1790
Size: 3127 1156 2573
Fragments arranged by size:
3127 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6856
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2804 4021 4587
Size: 416 53 83 399 24 1387 1217 566
Cuts at: 4587 5545 442
Size: 958 1753
Fragments arranged by size:
1753 1387 1217 958 566 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6856
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 3361 2878
Size: 483 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 2848 3096 3310 3331 3463 3610 5516
Size: 1068 541 248 214 21 132 147 1906
Cuts at: 5516 1239
Size: 2579
Fragments arranged by size:
2579 1906 1068 541 248 214 147 132
21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2524 3635 4981 5846 6144 2180
Size: 344 1111 1346 865 298 2892
Fragments arranged by size:
2892 1346 1111 865 344 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5519 882
Size: 617 4020 2219
Fragments arranged by size:
4020 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4153 4647 493
Size: 711 2949 494 2702
Fragments arranged by size:
2949 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6850 6850
Size: 6856
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4663 5045 5680 275
Size: 53 83 186 936 3130 382 635 1451
Fragments arranged by size:
3130 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5289 5687 5961 6108 6519 5289
Size: 398 274 147 411 5626
Fragments arranged by size:
5626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2528 2558 2959 2989 3308
Size: 30 165 30 1125 30 401 30 319
Cuts at: 3308 3338 1178
Size: 30 4696
Fragments arranged by size:
4696 1125 401 319 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2315 2318 2450 2490 2729 2732 2894
Size: 1126 3 3 132 40 239 3 162
Cuts at: 2894 3368 3371 3429 3503 3506 3758 3761 3834
Size: 474 3 58 74 3 252 3 73
Cuts at: 3834 3956 1186
Size: 122 4086
Fragments arranged by size:
4086 1126 474 252 239 162 132 122
74 73 58 40 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2054 2970 6059 6555 2054
Size: 916 3089 496 2355
Fragments arranged by size:
3089 2355 916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3573 3687 4020 1163
Size: 573 1837 114 333 3999
Fragments arranged by size:
3999 1837 573 333 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 4306 5067 6095 1540
Size: 275 186 30 2275 761 1028 2301
Fragments arranged by size:
2301 2275 1028 761 275 186 30
BsiHKAI G_wGCw'C
Cuts at: 2079 2522 3137 4917 5002 6073 2079
Size: 443 615 1780 85 1071 2862
Fragments arranged by size:
2862 1780 1071 615 443 85
BsmI GAATG_Cn'
Cuts at: 2037 4367 4460 2037
Size: 2330 93 4433
Fragments arranged by size:
4433 2330 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2522 3137 3665 4175 4917 5002
Size: 399 289 443 615 528 510 742 85
Cuts at: 5002 6073 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 615 528 510 443 399
289 85
BspEI T'CCGG_A
Cuts at: 6519 6519
Size: 6856
BspHI T'CATG_A
Cuts at: 4745 6475 4745
Size: 1730 5126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2353 3253 4211 844
Size: 1509 900 958 3489
Fragments arranged by size:
3489 1509 958 900
BsrBI CCG'CTC
Cuts at: 1394 1823 4743 1394
Size: 429 2920 3507
Fragments arranged by size:
3507 2920 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5351 5525 65
Size: 1183 4103 174 1396
Fragments arranged by size:
4103 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 5500 5687 1700
Size: 110 223 3467 187 2869
Fragments arranged by size:
3467 2869 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6856
BssSI C'ACGA_G
Cuts at: 2072 3963 4916 5928 2072
Size: 1891 953 1012 3000
Fragments arranged by size:
3000 1891 1012 953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3551 3710 794
Size: 2757 159 3940
Fragments arranged by size:
3940 2757 159
BstBI TT'CG_AA
Cuts at: 2007 5660 2007
Size: 3653 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstYI r'GATC_y
Cuts at: 1972 4186 4553 4938 4955 6407 6493 6505 6851
Size: 2214 367 385 17 1452 86 12 346
Cuts at: 6851 1972
Size: 1977
Fragments arranged by size:
2214 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3441 3846 3441
Size: 405 6451
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5347
Fragments arranged by size:
5347 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6117
Fragments arranged by size:
6117 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 2707 2857 3022 3034 3255 3490 3780 4372
Size: 294 150 165 12 221 235 290 592
Cuts at: 4372 5166 5186 2413
Size: 794 20 4083
Fragments arranged by size:
4083 794 592 294 290 235 221 165
150 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6821
DraI TTT'AAA
Cuts at: 4160 4160
Size: 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3511 5863 809
Size: 507 2195 2352 1802
Fragments arranged by size:
2352 2195 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2112 2799
Size: 54 1425 50 275 18 198 84 687
Cuts at: 2799 3289 3378 4303 5192 6821 8
Size: 490 89 925 889 1629 43
Fragments arranged by size:
1629 1425 925 889 687 490 275 198
89 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 4303 1537
Size: 275 216 2275 4090
Fragments arranged by size:
4090 2275 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 2170 2406 2834 2871 3051 3142 3330 3492
Size: 817 236 428 37 180 91 188 162
Cuts at: 3492 3630 3636 4786 1353
Size: 138 6 1150 3423
Fragments arranged by size:
3423 1150 817 428 236 188 180 162
138 91 37 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5443 5817 5963 387
Size: 1064 138 3854 374 146 1280
Fragments arranged by size:
3854 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2520 3135 2520
Size: 615 6241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2848 3072 3096 3123 3310 3331
Size: 393 675 541 224 24 27 187 21
Cuts at: 3331 3463 3610 3851 4919 5516 6303 6571 1239
Size: 132 147 241 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 787 675 597 541 393 268
241 224 187 147 132 27 24 21
EcoO109I rG'GnC_Cy
Cuts at: 2209 2318 2806 2912 2209
Size: 109 488 106 6153
Fragments arranged by size:
6153 488 109 106
EcoRI G'AATT_C
Cuts at: 3208 4165 4543 3208
Size: 957 378 5521
Fragments arranged by size:
5521 957 378
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 6003 1678
Size: 4325 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2528 2554 2560 3308
Size: 32 135 32 950 6 26 6 748
Cuts at: 3308 3340 3446 3478 3491 3523 4095 4127 5497
Size: 32 106 32 13 32 572 32 1370
Cuts at: 5497 5529 5571 5603 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1370 950 748 572 135 106 42
32 32 32 32 32 32 32 32
26 13 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4200 4446 669
Size: 988 281 2262 246 3079
Fragments arranged by size:
3079 2262 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3296 3863 748
Size: 2548 567 3741
Fragments arranged by size:
3741 2548 567
HpaI GTT'AAC
Cuts at: 4446 4446
Size: 6856
KpnI G_GTAC'C
Cuts at: 3201 4181 3201
Size: 980 5876
MfeI C'AATT_G
Cuts at: 4455 4455
Size: 6856
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5970 6154 1305
Size: 4665 184 2007
Fragments arranged by size:
4665 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 2801 3291 3380 10
Size: 54 1425 343 282 687 490 89 3486
Fragments arranged by size:
3486 1425 687 490 343 282 89 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4814 5173 5332 518
Size: 648 3648 359 159 2042
Fragments arranged by size:
3648 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3194 3227 3260 4272 4949 6097 6342 1606
Size: 1588 33 33 1012 677 1148 245 2120
Fragments arranged by size:
2120 1588 1148 1012 677 245 33 33
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 6856
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5347
Fragments arranged by size:
5347 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6856
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 6856
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6856
NotI GC'GGCC_GC
Cuts at: 2028 4303 2028
Size: 2275 4581
NspI r_CATG'y
Cuts at: 1132 1229 4214 5759 1132
Size: 97 2985 1545 2229
Fragments arranged by size:
2985 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6856
PciI A'CATG_T
Cuts at: 5755 5755
Size: 6856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6856
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2959 2991 4925 4957 6438 6470 2959
Size: 32 1934 32 1481 32 3345
Fragments arranged by size:
3345 1934 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2912 2209
Size: 703 6153
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 4426 4426
Size: 6856
PspXI vC'TCGA_Gb
Cuts at: 2631 2631
Size: 6856
PstI C_TGCA'G
Cuts at: 830 2383 3250 3265 3520 4208 830
Size: 1553 867 15 255 688 3478
Fragments arranged by size:
3478 1553 867 688 255 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 3194 3227 3260 3194
Size: 33 33 6790
Fragments arranged by size:
6790 33 33
SacI G_AGCT'C
Cuts at: 2522 3137 2522
Size: 615 6241
SalI G'TCGA_C
Cuts at: 1936 4198 1936
Size: 2262 4594
SapI GCTCTTCn'nnn_
Cuts at: 3142 3142
Size: 6856
SbfI CC_TGCA'GG
Cuts at: 4208 4208
Size: 6856
ScaI AGT'ACT
Cuts at: 5104 6816 5104
Size: 1712 5144
SexAI A'CCwGG_T
Cuts at: 2908 2908
Size: 6856
SfcI C'TryA_G
Cuts at: 826 1045 2182 2379 2917 3246 3261 3516 4204
Size: 219 1137 197 538 329 15 255 688
Cuts at: 4204 5339 6020 6211 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1137 1135 688 681 538 329 255
219 197 191 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 4183 1872
Size: 2311 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 2539 2631 3650 4960 5861
Size: 197 926 252 344 92 1019 1310 901
Cuts at: 5861 6123 820
Size: 262 1553
Fragments arranged by size:
1553 1310 1019 926 901 344 262 252
197 92
SnaBI TAC'GTA
Cuts at: 493 4153 4647 493
Size: 3660 494 2702
Fragments arranged by size:
3660 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6856
SphI G_CATG'C
Cuts at: 1132 4214 1132
Size: 3082 3774
SspI AAT'ATT
Cuts at: 5 52 4780 5
Size: 47 4728 2081
Fragments arranged by size:
4728 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 1517
Size: 750 6106
StyI C'CwwG_G
Cuts at: 513 1065 2022 2205 2484 2802 3393 3684 3828
Size: 552 957 183 279 318 591 291 144
Cuts at: 3828 4295 513
Size: 467 3074
Fragments arranged by size:
3074 957 591 552 467 318 291 279
183 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 4883 4900 5053 5238 5683 325
Size: 1255 768 2535 17 153 185 445 1498
Fragments arranged by size:
2535 1498 1255 768 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5102 6814 96
Size: 274 80 33 51 1443 3125 1712 138
Fragments arranged by size:
3125 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6816 6848 6816
Size: 32 6824
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6856
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2986 3277 2986
Size: 291 6565
XhoI C'TCGA_G
Cuts at: 1943 2631 1943
Size: 688 6168
XmaI C'CCGG_G
Cuts at: 1870 4181 1870
Size: 2311 4545
XmnI GAAnn'nnTTC
Cuts at: 4985 4985
Size: 6856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4664 276
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI