Restriction map for
pFN21ASDA0027
(Circular) (Six-base) MAPSORT of: pFN21ASDA0027.seq Check: 1590 from: 1 to: 5860
pFN21ASDA0027 5860 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 3119 3923 5307 5575 1632
Size: 514 973 804 1384 268 1917
Fragments arranged by size:
1917 1384 973 804 514 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2244 2393 4759 5736 1203
Size: 1041 149 2366 977 1327
Fragments arranged by size:
2366 1327 1041 977 149
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2305 1184
Size: 1121 4739
AlwNI CAG_nnn'CTG
Cuts at: 2849 3603 5175 2849
Size: 754 1572 3534
Fragments arranged by size:
3534 1572 754
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 2598 2654 3185 1870
Size: 73 275 380 56 531 4545
Fragments arranged by size:
4545 531 380 275 73 56
BamHI G'GATC_C
Cuts at: 2724 3190 3557 2724
Size: 466 367 5027
Fragments arranged by size:
5027 466 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2984 3181 4636 618
Size: 325 217 627 1197 197 1455 1842
Fragments arranged by size:
1842 1455 1197 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2552 3095 3179 1391
Size: 1161 543 84 4072
Fragments arranged by size:
4072 1161 543 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2173 928
Size: 971 274 4615
Fragments arranged by size:
4615 971 274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2113 2131 2147 2165 4051 4085 1903
Size: 34 176 18 16 18 1886 34 3678
Fragments arranged by size:
3678 1886 176 34 34 18 18 16
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 4906
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2987 3921 5077 1790
Size: 1197 934 1156 2573
Fragments arranged by size:
2573 1197 1156 934
BmgBI CAC'GTC
Cuts at: 1318 2175 2826 1318
Size: 857 651 4352
Fragments arranged by size:
4352 857 651
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3145 3591 4549
Size: 416 53 83 399 24 1728 446 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1728 958 446 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2308 2498 3145 4520 1239
Size: 857 212 190 647 1375 2579
Fragments arranged by size:
2579 1375 857 647 212 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2394 3157 3651 493
Size: 711 1190 763 494 2702
Fragments arranged by size:
2702 1190 763 711 494
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 4293 4691 4965 5112 5523 2061
Size: 2232 398 274 147 411 2398
Fragments arranged by size:
2398 2232 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2540 1186
Size: 874 480 4506
Fragments arranged by size:
4506 874 480
BseYI C'CCAG_C
Cuts at: 2780 3074 5063 5559 2780
Size: 294 1989 496 3081
Fragments arranged by size:
3081 1989 496 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2496 1163
Size: 573 760 4527
Fragments arranged by size:
4527 760 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2958 3921 4006 5077 2958
Size: 963 85 1071 3741
Fragments arranged by size:
3741 1071 963 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
BsmBI CGTCTCn'nnnn_
Cuts at: 2326 2832 2326
Size: 506 5354
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2552 2958 2987 3095 3179 3921 4006
Size: 399 762 406 29 108 84 742 85
Cuts at: 4006 5077 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 762 742 406 399 108 85
84 29
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 2871 3749 5479 2871
Size: 878 1730 3252
Fragments arranged by size:
3252 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 3215 844
Size: 1295 1076 3489
Fragments arranged by size:
3489 1295 1076
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 3747 1394
Size: 429 257 1667 3507
Fragments arranged by size:
3507 1667 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 2751 4355 4529 65
Size: 1183 1416 87 1604 174 1396
Fragments arranged by size:
1604 1416 1396 1183 174 87
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2969 4504 4691 1700
Size: 110 275 884 1535 187 2869
Fragments arranged by size:
2869 1535 884 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 2007 4664 2007
Size: 2657 3203
BstEII G'GTnAC_C
Cuts at: 1184 2212 1184
Size: 1028 4832
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 2764 2575
Size: 189 5671
BstYI r'GATC_y
Cuts at: 1972 2724 3190 3557 3942 3959 5411 5497 5509
Size: 752 466 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 752 466 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2496 513
Size: 552 1431 3877
Fragments arranged by size:
3877 1431 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2491 487
Size: 683 56 1265 3856
Fragments arranged by size:
3856 1265 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4170 4190 3376
Size: 794 20 5046
Fragments arranged by size:
5046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DraIII CAC_nnn'GTG
Cuts at: 2394 2394
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2493 3084 3307
Size: 54 1425 50 275 18 663 591 223
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 663 591 275 223 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 3790 1353
Size: 785 1652 3423
Fragments arranged by size:
3423 1652 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2146 2308 2498 3119 3145 3923
Size: 393 464 50 162 190 621 26 778
Cuts at: 3923 4520 5307 5575 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 778 621 597 464 393 268
190 162 50 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2963 2963
Size: 5860
EcoO109I rG'GnC_Cy
Cuts at: 2602 2800 2602
Size: 198 5662
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
EcoRV GAT'ATC
Cuts at: 2855 2855
Size: 5860
FseI GG_CCGG'CC
Cuts at: 2973 2973
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2568 3070 5007 1678
Size: 890 502 1937 2531
Fragments arranged by size:
2531 1937 890 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373
Size: 32 135 32 2929 32 42 32 2626
Fragments arranged by size:
2929 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 2304 3459 2304
Size: 1155 4705
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3086 10
Size: 54 1425 343 1254 2784
Fragments arranged by size:
2784 1425 1254 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3818 4177 4336 518
Size: 648 2652 359 159 2042
Fragments arranged by size:
2652 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2833 3276 3953 5101 5346 1606
Size: 531 696 443 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 531 443 245
NaeI GCC'GGC
Cuts at: 2087 2971 2087
Size: 884 4976
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2085 2969 2085
Size: 884 4976
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 2324 2324
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 2800 2602
Size: 198 5662
PshAI GACnn'nnGTC
Cuts at: 1613 2177 1613
Size: 564 5296
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2136 2284 2967 3030 3212 830
Size: 1306 148 683 63 182 3478
Fragments arranged by size:
3478 1306 683 182 148 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2132 2280 2963 3026 3208 4343 5024
Size: 219 1087 148 683 63 182 1135 681
Cuts at: 5024 5215 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1087 683 681 219 191 182
148 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3157 3651 493
Size: 2664 494 2702
Fragments arranged by size:
2702 2664 494
SpeI A'CTAG_T
Cuts at: 152 2419 152
Size: 2267 3593
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 3299 513
Size: 552 2234 3074
Fragments arranged by size:
3074 2234 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 3887 3904 4057 4242 4687 325
Size: 1255 1482 825 17 153 185 445 1498
Fragments arranged by size:
1498 1482 1255 825 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 2788
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 2246 3104 3989 2246
Size: 858 885 4117
Fragments arranged by size:
4117 885 858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI
FalI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI