Restriction map for
pFN21ASDA0024
(Circular) (Six-base) MAPSORT of: pFN21ASDA0024.seq Check: 1117 from: 1 to: 6148
pFN21ASDA0024 6148 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3360 4211 5595 5863 1632
Size: 1728 851 1384 268 1917
Fragments arranged by size:
1917 1728 1384 851 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3319 3351 3319
Size: 32 6116
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2386 2991 3131 3457 3683 3835 5944 1493
Size: 893 605 140 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 605 326 226 152 140
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3473 1870
Size: 73 1530 4545
Fragments arranged by size:
4545 1530 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3287 3469 4924 618
Size: 325 217 627 1500 182 1455 1842
Fragments arranged by size:
1842 1500 1455 627 325 217 182
BanII G_rGCy'C
Cuts at: 1391 2625 3467 1391
Size: 1234 842 4072
Fragments arranged by size:
4072 1234 842
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 3348 928
Size: 971 327 1122 3728
Fragments arranged by size:
3728 1122 971 327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 2091 2487 2904 1548
Size: 543 396 417 4792
Fragments arranged by size:
4792 543 417 396
BfrBI ATG'CAT
Cuts at: 2080 2635 2080
Size: 555 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 4759 136
Size: 107 122 71 631 605 886 2201 1525
Fragments arranged by size:
2201 1525 886 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3142 3303 6143 3142
Size: 161 2840 3147
Fragments arranged by size:
3147 2840 161
BlpI GC'TnA_GC
Cuts at: 2159 3565 2159
Size: 1406 4742
Bme1580I G_kGCm'C
Cuts at: 1790 4209 5365 1790
Size: 2419 1156 2573
Fragments arranged by size:
2573 2419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2770 3879 4837
Size: 416 53 83 399 24 1353 1109 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1753 1353 1109 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2835 1056
Size: 1779 4369
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 2258
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2251 2907 3131 4808 1239
Size: 1012 656 224 1677 2579
Fragments arranged by size:
2579 1677 1012 656 224
Bpu10I CC'TnA_GC
Cuts at: 2051 2051
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2588 4811 882
Size: 617 1089 2223 2219
Fragments arranged by size:
2223 2219 1089 617
BsaAI yAC'GTr
Cuts at: 493 1204 3445 3939 493
Size: 711 2241 494 2702
Fragments arranged by size:
2702 2241 711 494
BsaBI GATnn'nnATC
Cuts at: 2427 6142 2427
Size: 3715 2433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 3955 4337 4972
Size: 53 83 186 936 494 1928 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1928 1451 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2085 4581 4979 5253 5400 5811 2085
Size: 2496 398 274 147 411 2422
Fragments arranged by size:
2496 2422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5923
Fragments arranged by size:
5923 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 1186
Size: 1444 4704
BseYI C'CCAG_C
Cuts at: 2547 2716 3084 5351 5847 2547
Size: 169 368 2267 496 2848
Fragments arranged by size:
2848 2267 496 368 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2141 3598 4359 5387 1540
Size: 275 186 140 1457 761 1028 2301
Fragments arranged by size:
2301 1457 1028 761 275 186 140
BsiHKAI G_wGCw'C
Cuts at: 2352 2625 4209 4294 5365 2352
Size: 273 1584 85 1071 3135
Fragments arranged by size:
3135 1584 1071 273 85
BsmI GAATG_Cn'
Cuts at: 3403 3659 3752 3403
Size: 256 93 5799
Fragments arranged by size:
5799 256 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2625 3467 4209 4294 5365 1391
Size: 399 562 273 842 742 85 1071 2174
Fragments arranged by size:
2174 1071 842 742 562 399 273 85
BspEI T'CCGG_A
Cuts at: 2085 5811 2085
Size: 3726 2422
BspHI T'CATG_A
Cuts at: 2901 4037 5767 2901
Size: 1136 1730 3282
Fragments arranged by size:
3282 1730 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 3503 844
Size: 1542 1117 3489
Fragments arranged by size:
3489 1542 1117
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2704 4643 4817 65
Size: 1183 1456 1939 174 1396
Fragments arranged by size:
1939 1456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2733 4792 4979 1700
Size: 110 326 597 2059 187 2869
Fragments arranged by size:
2869 2059 597 326 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 2007 4952 2007
Size: 2945 3203
BstEII G'GTnAC_C
Cuts at: 1184 3251 1184
Size: 2067 4081
BstXI CCAn_nnnn'nTGG
Cuts at: 2322 2982 2322
Size: 660 5488
BstYI r'GATC_y
Cuts at: 1972 2422 3142 3303 3478 3845 4230 4247 5699
Size: 450 720 161 175 367 385 17 1452
Cuts at: 5699 5785 5797 6143 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 720 450 385 367 346 175
161 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4639
Fragments arranged by size:
4639 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3664 4458 4478 3664
Size: 794 20 5334
Fragments arranged by size:
5334 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2686 2721 529
Size: 35 2122 35 3956
Fragments arranged by size:
3956 2122 35 35
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DraIII CAC_nnn'GTG
Cuts at: 3060 3060
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2138 3595 4484
Size: 54 1425 50 275 18 308 1457 889
Cuts at: 4484 6113 8
Size: 1629 43
Fragments arranged by size:
1629 1457 1425 889 308 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2138 3595 1537
Size: 275 326 1457 4090
Fragments arranged by size:
4090 1457 326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 2630 3152 4078 1353
Size: 1031 246 522 926 3423
Fragments arranged by size:
3423 1031 926 522 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2623 2623
Size: 6148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 2907 3131 3360 4211 4808 5595
Size: 393 619 656 224 229 851 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1524 851 787 656 619 597 393 268
229 224
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2197 2497 2054
Size: 143 300 5705
Fragments arranged by size:
5705 300 143
EcoRI G'AATT_C
Cuts at: 2991 3457 3835 2991
Size: 466 378 5304
Fragments arranged by size:
5304 466 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2968 3399 2968
Size: 431 5717
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 5295 1678
Size: 3617 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2849 2881 3129 3134 3161
Size: 32 135 32 1277 32 248 5 27
Cuts at: 3161 3166 3382 3414 4789 4821 4863 4895 1373
Size: 5 216 32 1375 32 42 32 2626
Fragments arranged by size:
2626 1375 1277 248 216 135 42 32
32 32 32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2767 748
Size: 2019 4129
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3412 5262 5446 1305
Size: 2107 1850 184 2007
Fragments arranged by size:
2107 2007 1850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 4106 4465 4624 518
Size: 648 1107 1833 359 159 2042
Fragments arranged by size:
2042 1833 1107 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2917 3564 4241 5389 5634 1606
Size: 1311 647 677 1148 245 2120
Fragments arranged by size:
2120 1311 1148 677 647 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4639
Fragments arranged by size:
4639 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 2831 1052
Size: 1779 4369
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 2082 2637 2082
Size: 555 5593
NspI r_CATG'y
Cuts at: 1132 1229 3362 3506 5051 1132
Size: 97 2133 144 1545 2229
Fragments arranged by size:
2229 2133 1545 144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PfoI T'CCnGG_A
Cuts at: 3108 3386 3108
Size: 278 5870
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2820 3500 830
Size: 1990 680 3478
Fragments arranged by size:
3478 1990 680
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2917 2917
Size: 6148
SacI G_AGCT'C
Cuts at: 2625 2625
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2630 2630
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 3035 4396 6108 3035
Size: 1361 1712 3075
Fragments arranged by size:
3075 1712 1361
SfcI C'TryA_G
Cuts at: 826 1045 2816 3292 3496 4631 5312 5503 826
Size: 219 1771 476 204 1135 681 191 1471
Fragments arranged by size:
1771 1471 1135 681 476 219 204 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2558 2558
Size: 6148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3445 3939 493
Size: 2952 494 2702
Fragments arranged by size:
2952 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3362 3506 1132
Size: 2230 144 3774
Fragments arranged by size:
3774 2230 144
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 2561 1517
Size: 1044 5104
StyI C'CwwG_G
Cuts at: 513 1065 2022 3587 513
Size: 552 957 1565 3074
Fragments arranged by size:
3074 1565 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3076 3367 4175 4192 4345 4530 4975
Size: 1255 1496 291 808 17 153 185 445
Cuts at: 4975 325
Size: 1498
Fragments arranged by size:
1498 1496 1255 808 445 291 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3033 4394
Size: 274 80 33 51 1443 207 849 1361
Cuts at: 4394 6106 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1361 849 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2331 2363 3280 3312 6108 6140 2331
Size: 32 917 32 2796 32 2339
Fragments arranged by size:
2796 2339 917 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3306 3306
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 2933 4277 2933
Size: 1344 4804
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI
SrfI SwaI