Restriction map for
pFN21ASDA0023
(Circular) (Six-base) MAPSORT of: pFN21ASDA0023.seq Check: 2885 from: 1 to: 11008
pFN21ASDA0023 11008 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3418 4542 6718 3418
Size: 1124 2176 7708
Fragments arranged by size:
7708 2176 1124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4994 6056 8818 278
Size: 53 83 186 936 3458 1062 2762 2468
Fragments arranged by size:
3458 2762 2468 1062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3123 8351 1937
Size: 1186 5228 4594
Fragments arranged by size:
5228 4594 1186
Acc65I G'GTAC_C
Cuts at: 2771 6866 7100 7421 8329 2771
Size: 4095 234 321 908 5450
Fragments arranged by size:
5450 4095 908 321 234
AclI AA'CG_TT
Cuts at: 1563 5333 9135 9508 1563
Size: 3770 3802 373 3063
Fragments arranged by size:
3802 3770 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 4406 4619 5127 5514 7177 7461 7600
Size: 2393 381 213 508 387 1663 284 139
Cuts at: 7600 8084 9071 10455 10723 1632
Size: 484 987 1384 268 1917
Fragments arranged by size:
2393 1917 1663 1384 987 508 484 387
381 284 268 213 139
AfeI AGC'GCT
Cuts at: 2085 2085
Size: 11008
AflII C'TTAA_G
Cuts at: 820 1017 5474 820
Size: 197 4457 6354
Fragments arranged by size:
6354 4457 197
AflIII A'CryG_T
Cuts at: 1203 9907 10884 1203
Size: 8704 977 1327
Fragments arranged by size:
8704 1327 977
AgeI A'CCGG_T
Cuts at: 9839 9839
Size: 11008
AhdI GACnn_n'nnGTC
Cuts at: 9737 9737
Size: 11008
AleI CACnn'nnGTG
Cuts at: 1166 3704 6974 1166
Size: 2538 3270 5200
Fragments arranged by size:
5200 3270 2538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3722 5280 8093 1184
Size: 2538 1558 2813 4099
Fragments arranged by size:
4099 2813 2538 1558
AlwNI CAG_nnn'CTG
Cuts at: 2981 3361 3386 3566 4091 4507 4969 5435 5621
Size: 380 25 180 525 416 462 466 186
Cuts at: 5621 6755 6776 6956 7052 8751 10323 2981
Size: 1134 21 180 96 1699 1572 3666
Fragments arranged by size:
3666 1699 1572 1134 525 466 462 416
380 186 180 180 96 25 21
ApaLI G'TGCA_C
Cuts at: 5223 9065 10221 5223
Size: 3842 1156 6010
Fragments arranged by size:
6010 3842 1156
ApoI r'AATT_y
Cuts at: 1493 2297 4428 4454 5467 8317 8543 8695 10804
Size: 804 2131 26 1013 2850 226 152 2109
Cuts at: 10804 1493
Size: 1697
Fragments arranged by size:
2850 2131 2109 1697 1013 804 226 152
26
AseI AT'TA_AT
Cuts at: 160 1032 3030 3234 9562 160
Size: 872 1998 204 6328 1606
Fragments arranged by size:
6328 1998 1606 872 204
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11008
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2137 8333 1870
Size: 73 108 86 6196 4545
Fragments arranged by size:
6196 4545 108 86 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2763 2796 2763
Size: 33 10975
BamHI G'GATC_C
Cuts at: 4399 6218 7691 7910 8338 8705 4399
Size: 1819 1473 219 428 367 6702
Fragments arranged by size:
6702 1819 1473 428 367 219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2771 4434 4530 5302 5848
Size: 325 217 627 984 1663 96 772 546
Cuts at: 5848 6866 7100 7277 7421 7930 8329 9784 618
Size: 1018 234 177 144 509 399 1455 1842
Fragments arranged by size:
1842 1663 1455 1018 984 772 627 546
509 399 325 234 217 177 144 96
BanII G_rGCy'C
Cuts at: 1391 2627 2901 3457 3519 6117 7976 8327 1391
Size: 1236 274 556 62 2598 1859 351 4072
Fragments arranged by size:
4072 2598 1859 1236 556 351 274 62
BbeI G_GCGC'C
Cuts at: 4438 4438
Size: 11008
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2167 2773 4625 4859 4932 5649 7317
Size: 971 268 606 1852 234 73 717 1668
Cuts at: 7317 928
Size: 4619
Fragments arranged by size:
4619 1852 1668 971 717 606 268 234
73
BbvCI CC'TCA_GC
Cuts at: 7426 7426
Size: 11008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2021 2055 7975 8009 9199 9233 1903
Size: 34 84 34 5920 34 1190 34 3678
Fragments arranged by size:
5920 3678 1190 84 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3014 8900 10110 3014
Size: 5886 1210 3912
Fragments arranged by size:
5886 3912 1210
BclI T'GATC_A
Cuts at: 1548 3022 1548
Size: 1474 9534
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5258 5300 5485
Size: 107 122 71 631 605 3586 42 185
Cuts at: 5485 7971 9619 136
Size: 2486 1648 1525
Fragments arranged by size:
3586 2486 1648 1525 631 605 185 122
107 71 42
BglII A'GATC_T
Cuts at: 11003 11003
Size: 11008
BlpI GC'TnA_GC
Cuts at: 4416 6897 6922 8425 4416
Size: 2481 25 1503 6999
Fragments arranged by size:
6999 2481 1503 25
Bme1580I G_kGCm'C
Cuts at: 1790 3969 4187 5227 6066 6312 8006 9069 10225
Size: 2179 218 1040 839 246 1694 1063 1156
Cuts at: 10225 1790
Size: 2573
Fragments arranged by size:
2573 2179 1694 1156 1063 1040 839 246
218
BmgBI CAC'GTC
Cuts at: 1318 6822 1318
Size: 5504 5504
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 3956 4957
Size: 416 53 83 399 24 2102 437 1001
Cuts at: 4957 5061 5411 5423 7632 8739 9697 442
Size: 104 350 12 2209 1107 958 1753
Fragments arranged by size:
2209 2102 1753 1107 1001 958 437 416
399 350 104 83 53 24 12
BmtI G_CTAG'C
Cuts at: 1056 5163 1056
Size: 4107 6901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4431 8070 4431
Size: 3639 7369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 2811 3071 3165 3987 4863 4991 5961
Size: 1402 170 260 94 822 876 128 970
Cuts at: 5961 6796 7285 7827 7863 9668 1239
Size: 835 489 542 36 1805 2579
Fragments arranged by size:
2579 1805 1402 970 876 835 822 542
489 260 170 128 94 36
Bpu10I CC'TnA_GC
Cuts at: 3945 7075 7426 3945
Size: 3130 351 7527
Fragments arranged by size:
7527 3130 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2341 3330 4328 5160 5334 6748 7270 9133 9998
Size: 989 998 832 174 1414 522 1863 865
Cuts at: 9998 10296 2341
Size: 298 3053
Fragments arranged by size:
3053 1863 1414 998 989 865 832 522
298 174
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4250 5463 6493 9671 882
Size: 617 2751 1213 1030 3178 2219
Fragments arranged by size:
3178 2751 2219 1213 1030 617
BsaAI yAC'GTr
Cuts at: 493 1204 8305 8799 493
Size: 711 7101 494 2702
Fragments arranged by size:
7101 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4086 4503 11002 4086
Size: 417 6499 4092
Fragments arranged by size:
6499 4092 417
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4435 4991 6053 8815
Size: 53 83 186 936 2902 556 1062 2762
Cuts at: 8815 9197 9832 275
Size: 382 635 1451
Fragments arranged by size:
2902 2762 1451 1062 936 635 556 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5501 9441 9839 10113 10260 10671 5501
Size: 3940 398 274 147 411 5838
Fragments arranged by size:
5838 3940 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2460 2490 3105 3135 3157
Size: 30 165 30 1057 30 615 30 22
Cuts at: 3157 3187 6509 6539 7395 7425 7803 7833 7974
Size: 30 3322 30 856 30 378 30 141
Cuts at: 7974 8004 1178
Size: 30 4182
Fragments arranged by size:
4182 3322 1057 856 615 378 165 141
30 30 30 30 30 30 30 30
30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3331 4127 4208 6530 6537 7824 8115 1186
Size: 2145 796 81 2322 7 1287 291 4079
Fragments arranged by size:
4079 2322 2145 1287 796 291 81 7
BseYI C'CCAG_C
Cuts at: 5908 6039 6531 6591 6664 7090 7951 10211 10707
Size: 131 492 60 73 426 861 2260 496
Cuts at: 10707 5908
Size: 6209
Fragments arranged by size:
6209 2260 861 496 492 426 131 73
60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2899 5384 5658 6234 6789 1163
Size: 573 1163 2485 274 576 555 5382
Fragments arranged by size:
5382 2485 1163 576 573 555 274
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8458 9219 10247 1540
Size: 275 186 6457 761 1028 2301
Fragments arranged by size:
6457 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5227 9069 9154 10225 5227
Size: 3842 85 1071 6010
Fragments arranged by size:
6010 3842 1071 85
BsmI GAATG_Cn'
Cuts at: 4704 5720 7937 8252 8519 8612 4704
Size: 1016 2217 315 267 93 7100
Fragments arranged by size:
7100 2217 1016 315 267 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2022 2829 3636 2022
Size: 807 807 9394
Fragments arranged by size:
9394 807 807
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2627 2901 3457 3519 3969 4187 5227
Size: 399 837 274 556 62 450 218 1040
Cuts at: 5227 6066 6117 6312 7976 8006 8327 9069 9154
Size: 839 51 195 1664 30 321 742 85
Cuts at: 9154 10225 1391
Size: 1071 2174
Fragments arranged by size:
2174 1664 1071 1040 839 837 742 556
450 399 321 274 218 195 85 62
51 30
BspEI T'CCGG_A
Cuts at: 10671 10671
Size: 11008
BspHI T'CATG_A
Cuts at: 8897 10627 8897
Size: 1730 9278
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3418 4542 5830 6718 8363 844
Size: 2574 1124 1288 888 1645 3489
Fragments arranged by size:
3489 2574 1645 1288 1124 888
BsrBI CCG'CTC
Cuts at: 1394 1823 2056 8120 8895 1394
Size: 429 233 6064 775 3507
Fragments arranged by size:
6064 3507 775 429 233
BsrDI GCAATG_nn'
Cuts at: 65 1248 3420 3741 4747 9503 9677 65
Size: 1183 2172 321 1006 4756 174 1396
Fragments arranged by size:
4756 2172 1396 1183 1006 321 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9652 9839 1700
Size: 110 7842 187 2869
Fragments arranged by size:
7842 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11008
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11008
BssSI C'ACGA_G
Cuts at: 9068 10080 9068
Size: 1012 9996
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2981 3386 5093 6650 794
Size: 2187 405 1707 1557 5152
Fragments arranged by size:
5152 2187 1707 1557 405
BstBI TT'CG_AA
Cuts at: 2007 9812 2007
Size: 7805 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11008
BstXI CCAn_nnnn'nTGG
Cuts at: 7835 7835
Size: 11008
BstYI r'GATC_y
Cuts at: 1972 4087 4399 4868 6218 7691 7910 8147 8338
Size: 2115 312 469 1350 1473 219 237 191
Cuts at: 8338 8705 9090 9107 10559 10645 10657 11003 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2115 1977 1473 1452 1350 469 385 367
346 312 237 219 191 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2115 5782 6067 2115
Size: 3667 285 7056
Fragments arranged by size:
7056 3667 285
BtgI C'CryG_G
Cuts at: 513 1065 2022 2511 2595 3108 5040 6672 513
Size: 552 957 489 84 513 1932 1632 4849
Fragments arranged by size:
4849 1932 1632 957 552 513 489 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10269
Fragments arranged by size:
10269 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 4872 5019 5667 6615 6954 7177 8004 8524
Size: 2220 147 648 948 339 223 827 520
Cuts at: 8524 9318 9338 2652
Size: 794 20 4322
Fragments arranged by size:
4322 2220 948 827 794 648 520 339
223 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3120 3155 6808 6843 7843 7878 529
Size: 35 2556 35 3653 35 1000 35 3659
Fragments arranged by size:
3659 3653 2556 1000 35 35 35 35
DraI TTT'AAA
Cuts at: 8312 8312
Size: 11008
DraIII CAC_nnn'GTG
Cuts at: 4624 5129 5419 4624
Size: 505 290 10213
Fragments arranged by size:
10213 505 290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4264 6485 10015 809
Size: 507 2948 2221 3530 1802
Fragments arranged by size:
3530 2948 2221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4842 5292 5977
Size: 54 1425 50 275 18 3012 450 685
Cuts at: 5977 6780 7354 7546 7674 8040 8155 8455 9344
Size: 803 574 192 128 366 115 300 889
Cuts at: 9344 10973 8
Size: 1629 43
Fragments arranged by size:
3012 1629 1425 889 803 685 574 450
366 300 275 192 128 115 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8455 1537
Size: 275 6643 4090
Fragments arranged by size:
6643 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2592 3174 3832 4137 4601 6435 7567 7594
Size: 1239 582 658 305 464 1834 1132 27
Cuts at: 7594 8938 1353
Size: 1344 3423
Fragments arranged by size:
3423 1834 1344 1239 1132 658 582 464
305 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4634 9595 9969 10115 387
Size: 1064 138 3045 4961 374 146 1280
Fragments arranged by size:
4961 3045 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2641 2811 3071 3165 3987 4025 4406
Size: 393 1009 170 260 94 822 38 381
Cuts at: 4406 4619 4863 4991 5127 5514 5961 6796 7177
Size: 213 244 128 136 387 447 835 381
Cuts at: 7177 7285 7461 7600 7827 7863 8084 9071 9668
Size: 108 176 139 227 36 221 987 597
Cuts at: 9668 10455 10723 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1009 987 835 822 787 597 447
393 387 381 381 268 260 244 227
221 213 176 170 139 136 128 108
94 38 36
EcoNI CCTnn'n_nnAGG
Cuts at: 5781 5781
Size: 11008
EcoO109I rG'GnC_Cy
Cuts at: 2811 3563 5023 6679 6773 8034 8293 2811
Size: 752 1460 1656 94 1261 259 5526
Fragments arranged by size:
5526 1656 1460 1261 752 259 94
EcoRI G'AATT_C
Cuts at: 8317 8695 8317
Size: 378 10630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3763 3797 4436 5763 3763
Size: 34 639 1327 9008
Fragments arranged by size:
9008 1327 639 34
FspI TGC'GCA
Cuts at: 9514 9514
Size: 11008
HaeII r_GCGC'y
Cuts at: 1678 2087 4438 10155 1678
Size: 409 2351 5717 2531
Fragments arranged by size:
5717 2531 2351 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2914 2946 3157 3189 3985
Size: 32 135 32 1342 32 211 32 796
Cuts at: 3985 4017 4600 4632 5281 5313 5708 5740 6509
Size: 32 583 32 649 32 395 32 769
Cuts at: 6509 6541 7395 7427 8132 8164 9649 9681 9723
Size: 32 854 32 705 32 1485 32 42
Cuts at: 9723 9755 1373
Size: 32 2626
Fragments arranged by size:
2626 1485 1342 854 796 769 705 649
583 395 211 135 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3778 5424 8352 8598 669
Size: 988 281 1840 1646 2928 246 3079
Fragments arranged by size:
3079 2928 1840 1646 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 5526 7473 748
Size: 1702 3076 1947 4283
Fragments arranged by size:
4283 3076 1947 1702
HpaI GTT'AAC
Cuts at: 8598 8598
Size: 11008
KasI G'GCGC_C
Cuts at: 4434 4434
Size: 11008
KpnI G_GTAC'C
Cuts at: 2775 6870 7104 7425 8333 2775
Size: 4095 234 321 908 5450
Fragments arranged by size:
5450 4095 908 321 234
MfeI C'AATT_G
Cuts at: 8607 8607
Size: 11008
MluI A'CGCG_T
Cuts at: 10884 10884
Size: 11008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 3564 4119 6377 8081 10122 10306 1305
Size: 876 1383 555 2258 1704 2041 184 2007
Fragments arranged by size:
2258 2041 2007 1704 1383 876 555 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4844 5294 5979 7356 7548
Size: 54 1425 343 3012 450 685 1377 192
Cuts at: 7548 8042 8157 10
Size: 494 115 2861
Fragments arranged by size:
3012 2861 1425 1377 685 494 450 343
192 115 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3704 4446 6974 8966 9325 9484 518
Size: 648 2538 742 2528 1992 359 159 2042
Fragments arranged by size:
2538 2528 2042 1992 742 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2641 2913 3377 3650 3845 4007 5264 5309
Size: 1035 272 464 273 195 162 1257 45
Cuts at: 5309 5555 6338 6425 6755 7052 7430 8012 8424
Size: 246 783 87 330 297 378 582 412
Cuts at: 8424 9101 10249 10494 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 1035 783 677 582 464
412 378 330 297 273 272 246 245
195 162 87 45
NarI GG'CG_CC
Cuts at: 4435 4435
Size: 11008
NcoI C'CATG_G
Cuts at: 513 1065 2022 2511 3108 513
Size: 552 957 489 597 8413
Fragments arranged by size:
8413 957 597 552 489
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11008
NheI G'CTAG_C
Cuts at: 1052 5159 1052
Size: 4107 6901
NotI GC'GGCC_GC
Cuts at: 8455 8455
Size: 11008
NruI TCG'CGA
Cuts at: 8238 8238
Size: 11008
NspI r_CATG'y
Cuts at: 1132 1229 8366 9911 1132
Size: 97 7137 1545 2229
Fragments arranged by size:
7137 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 7112 7916 1348
Size: 5764 804 4440
Fragments arranged by size:
5764 4440 804
PciI A'CATG_T
Cuts at: 9907 9907
Size: 11008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 3566 4969 7097 7886 1108
Size: 1206 1252 1403 2128 789 4230
Fragments arranged by size:
4230 2128 1403 1252 1206 789
PmeI GTTT'AAAC
Cuts at: 8312 8312
Size: 11008
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9077 9109 10590 10622 9077
Size: 32 1481 32 9463
Fragments arranged by size:
9463 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2811 6679 8034 8293 2811
Size: 3868 1355 259 5526
Fragments arranged by size:
5526 3868 1355 259
PshAI GACnn'nnGTC
Cuts at: 1613 6743 1613
Size: 5130 5878
PsiI TTA'TAA
Cuts at: 4386 8578 4386
Size: 4192 6816
PspXI vC'TCGA_Gb
Cuts at: 2137 2137
Size: 11008
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7288 7320 7486 7518 7288
Size: 32 166 32 10778
Fragments arranged by size:
10778 166 32 32
PstI C_TGCA'G
Cuts at: 830 2806 3376 3382 3628 6601 6622 6655 6850
Size: 1976 570 6 246 2973 21 33 195
Cuts at: 6850 8011 8360 830
Size: 1161 349 3478
Fragments arranged by size:
3478 2973 1976 1161 570 349 246 195
33 21 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11008
PvuII CAG'CTG
Cuts at: 2641 2913 3377 3650 3845 5264 5309 5555 6338
Size: 272 464 273 195 1419 45 246 783
Cuts at: 6338 6755 7052 7430 8012 2641
Size: 417 297 378 582 5637
Fragments arranged by size:
5637 1419 783 582 464 417 378 297
273 272 246 195 45
SalI G'TCGA_C
Cuts at: 1936 8350 1936
Size: 6414 4594
SanDI GG'GwC_CC
Cuts at: 2811 6679 8034 8293 2811
Size: 3868 1355 259 5526
Fragments arranged by size:
5526 3868 1355 259
SbfI CC_TGCA'GG
Cuts at: 8360 8360
Size: 11008
ScaI AGT'ACT
Cuts at: 3322 6631 7528 9256 10968 3322
Size: 3309 897 1728 1712 3362
Fragments arranged by size:
3362 3309 1728 1712 897
SexAI A'CCwGG_T
Cuts at: 4620 5106 5125 4620
Size: 486 19 10503
Fragments arranged by size:
10503 486 19
SfcI C'TryA_G
Cuts at: 826 1045 2802 3372 3378 3624 5397 6333 6597
Size: 219 1757 570 6 246 1773 936 264
Cuts at: 6597 6618 6651 6846 8007 8356 9491 10172 10363
Size: 21 33 195 1161 349 1135 681 191
Cuts at: 10363 826
Size: 1471
Fragments arranged by size:
1773 1757 1471 1161 1135 936 681 570
349 264 246 219 195 191 33 21
6
SfoI GGC'GCC
Cuts at: 4436 4436
Size: 11008
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11008
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11008
SmaI CCC'GGG
Cuts at: 1872 8335 1872
Size: 6463 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 2320 3345 4307 5175 5349
Size: 197 926 194 183 1025 962 868 174
Cuts at: 5349 5474 6763 7285 9112 10013 10275 820
Size: 125 1289 522 1827 901 262 1553
Fragments arranged by size:
1827 1553 1289 1025 962 926 901 868
522 262 197 194 183 174 125
SnaBI TAC'GTA
Cuts at: 493 8305 8799 493
Size: 7812 494 2702
Fragments arranged by size:
7812 2702 494
SpeI A'CTAG_T
Cuts at: 152 6032 6269 6632 152
Size: 5880 237 363 4528
Fragments arranged by size:
5880 4528 363 237
SphI G_CATG'C
Cuts at: 1132 8366 1132
Size: 7234 3774
SspI AAT'ATT
Cuts at: 5 52 8932 5
Size: 47 8880 2081
Fragments arranged by size:
8880 2081 47
StuI AGG'CCT
Cuts at: 1517 7145 1517
Size: 5628 5380
StyI C'CwwG_G
Cuts at: 513 1065 2022 2127 2275 2511 3108 7624 7828
Size: 552 957 105 148 236 597 4516 204
Cuts at: 7828 8091 8152 8447 513
Size: 263 61 295 3074
Fragments arranged by size:
4516 3074 957 597 552 295 263 236
204 148 105 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2886 4003 5430 7217 9035 9052 9205
Size: 1255 1306 1117 1427 1787 1818 17 153
Cuts at: 9205 9390 9835 325
Size: 185 445 1498
Fragments arranged by size:
1818 1787 1498 1427 1306 1255 1117 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3320 6629 7526
Size: 274 80 33 51 1443 1343 3309 897
Cuts at: 7526 9254 10966 96
Size: 1728 1712 138
Fragments arranged by size:
3309 1728 1712 1443 1343 897 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2460 2492 2747 2779 4721 4753 4763 4795 5130
Size: 32 255 32 1942 32 10 32 335
Cuts at: 5130 5162 6334 6366 7803 7835 7974 8006 10968
Size: 32 1172 32 1437 32 139 32 2962
Cuts at: 10968 11000 2460
Size: 32 2468
Fragments arranged by size:
2962 2468 1942 1437 1172 335 255 139
32 32 32 32 32 32 32 32
32 10
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11008
XbaI T'CTAG_A
Cuts at: 4483 6511 8344 4483
Size: 2028 1833 7147
Fragments arranged by size:
7147 2028 1833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 8196 8280 8196
Size: 84 10924
XhoI C'TCGA_G
Cuts at: 1943 2137 1943
Size: 194 10814
XmaI C'CCGG_G
Cuts at: 1870 8333 1870
Size: 6463 4545
XmnI GAAnn'nnTTC
Cuts at: 5959 6582 9137 5959
Size: 623 2555 7830
Fragments arranged by size:
7830 2555 623
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4992 6054 8816 276
Size: 53 83 186 936 3458 1062 2762 2468
Fragments arranged by size:
3458 2762 2468 1062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PfoI
PmlI PspOMI RsrII SacI SacII SapI SfiI SrfI
SwaI