Restriction map for
pFN21ASDA0019
(Circular) (Six-base) MAPSORT of: pFN21ASDA0019.seq Check: 4427 from: 1 to: 7213
pFN21ASDA0019 7213 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3501 5023 278
Size: 53 83 186 936 1965 1522 2468
Fragments arranged by size:
2468 1965 1522 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4556 1937
Size: 2619 4594
Acc65I G'GTAC_C
Cuts at: 4534 4534
Size: 7213
AclI AA'CG_TT
Cuts at: 1563 5340 5713 1563
Size: 3777 373 3063
Fragments arranged by size:
3777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3032 3137 5276 6660 6928 1632
Size: 1400 105 2139 1384 268 1917
Fragments arranged by size:
2139 1917 1400 1384 268 105
AflII C'TTAA_G
Cuts at: 820 1017 2768 820
Size: 197 1751 5265
Fragments arranged by size:
5265 1751 197
AflIII A'CryG_T
Cuts at: 1203 6112 7089 1203
Size: 4909 977 1327
Fragments arranged by size:
4909 1327 977
AgeI A'CCGG_T
Cuts at: 6044 6044
Size: 7213
AhdI GACnn_n'nnGTC
Cuts at: 5942 5942
Size: 7213
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2889 2921 2889
Size: 32 7181
AlwNI CAG_nnn'CTG
Cuts at: 3098 3587 4364 4956 6528 3098
Size: 489 777 592 1572 3783
Fragments arranged by size:
3783 1572 777 592 489
ApaI G_GGCC'C
Cuts at: 2356 2647 4443 2356
Size: 291 1796 5126
Fragments arranged by size:
5126 1796 291
ApaLI G'TGCA_C
Cuts at: 5270 6426 5270
Size: 1156 6057
ApoI r'AATT_y
Cuts at: 1493 2026 2310 2753 2984 4522 4748 4900 7009
Size: 533 284 443 231 1538 226 152 2109
Cuts at: 7009 1493
Size: 1697
Fragments arranged by size:
2109 1697 1538 533 443 284 231 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5767 160
Size: 872 4735 1606
Fragments arranged by size:
4735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 3778 3891 4281 4538 1870
Size: 73 1353 482 113 390 257 4545
Fragments arranged by size:
4545 1353 482 390 257 113 73
BamHI G'GATC_C
Cuts at: 4543 4910 4543
Size: 367 6846
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3241 4534 5989 618
Size: 325 217 627 1454 1293 1455 1842
Fragments arranged by size:
1842 1455 1454 1293 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2356 2647 3212 3659 3752 4443 4532
Size: 799 166 291 565 447 93 691 89
Cuts at: 4532 1391
Size: 4072
Fragments arranged by size:
4072 799 691 565 447 291 166 93
89
BbeI G_GCGC'C
Cuts at: 3245 3245
Size: 7213
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6242
BbvCI CC'TCA_GC
Cuts at: 3639 3639
Size: 7213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2615 2649 5404 5438 1903
Size: 34 678 34 2755 34 3678
Fragments arranged by size:
3678 2755 678 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3829 5105 6315 3829
Size: 1276 1210 4727
Fragments arranged by size:
4727 1276 1210
BclI T'GATC_A
Cuts at: 1548 4188 1548
Size: 2640 4573
BfrBI ATG'CAT
Cuts at: 2683 2683
Size: 7213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2642 3133 5824
Size: 107 122 71 631 605 970 491 2691
Cuts at: 5824 136
Size: 1525
Fragments arranged by size:
2691 1525 970 631 605 491 122 107
71
BglII A'GATC_T
Cuts at: 7208 7208
Size: 7213
BlpI GC'TnA_GC
Cuts at: 3792 4630 3792
Size: 838 6375
Bme1580I G_kGCm'C
Cuts at: 1790 2356 2647 3477 4443 5274 6430 1790
Size: 566 291 830 966 831 1156 2573
Fragments arranged by size:
2573 1156 966 831 830 566 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4944 5902 442
Size: 416 53 83 399 24 3527 958 1753
Fragments arranged by size:
3527 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3902 4293 1056
Size: 2846 391 3976
Fragments arranged by size:
3976 2846 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 4434 5873 1239
Size: 1076 2119 1439 2579
Fragments arranged by size:
2579 2119 1439 1076
Bpu10I CC'TnA_GC
Cuts at: 3639 3639
Size: 7213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2737 2772 3196 5338 6203 6501 2384
Size: 353 35 424 2142 865 298 3096
Fragments arranged by size:
3096 2142 865 424 353 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2990 4021 5876 882
Size: 617 1491 1031 1855 2219
Fragments arranged by size:
2219 1855 1491 1031 617
BsaAI yAC'GTr
Cuts at: 493 1204 4510 5004 493
Size: 711 3306 494 2702
Fragments arranged by size:
3306 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3114 7207 3114
Size: 4093 3120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3165 3242 3293 3498
Size: 53 83 186 936 1632 77 51 205
Cuts at: 3498 5020 5402 6037 275
Size: 1522 382 635 1451
Fragments arranged by size:
1632 1522 1451 936 635 382 205 186
83 77 53 51
BsaWI w'CCGG_w
Cuts at: 4063 5646 6044 6318 6465 6876 4063
Size: 1583 398 274 147 411 4400
Fragments arranged by size:
4400 1583 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2889 2919 3248 3278 1178
Size: 30 165 30 1486 30 329 30 5113
Fragments arranged by size:
5113 1486 329 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2198 2600 3205 3347 3350 3734 1186
Size: 1009 3 402 605 142 3 384 4665
Fragments arranged by size:
4665 1009 605 402 384 142 3 3
BseYI C'CCAG_C
Cuts at: 3773 3873 4142 6416 6912 3773
Size: 100 269 2274 496 4074
Fragments arranged by size:
4074 2274 496 269 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2127 1163
Size: 573 391 6249
Fragments arranged by size:
6249 573 391
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3206 4401 4663 5424 6452 1540
Size: 275 186 1205 1195 262 761 1028 2301
Fragments arranged by size:
2301 1205 1195 1028 761 275 262 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2425 3264 3307 5274 5359 6430 2190
Size: 235 839 43 1967 85 1071 2973
Fragments arranged by size:
2973 1967 1071 839 235 85 43
BsmI GAATG_Cn'
Cuts at: 4724 4817 4724
Size: 93 7120
BsmBI CGTCTCn'nnnn_
Cuts at: 3505 4253 3505
Size: 748 6465
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2356 2425 2647 3212 3264 3307
Size: 399 400 166 69 222 565 52 43
Cuts at: 3307 3477 3659 3752 4443 4532 5274 5359 6430
Size: 170 182 93 691 89 742 85 1071
Cuts at: 6430 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 691 565 400 399 222
182 170 166 93 89 85 69 52
43
BspEI T'CCGG_A
Cuts at: 4063 6876 4063
Size: 2813 4400
BspHI T'CATG_A
Cuts at: 2734 5102 6832 2734
Size: 2368 1730 3115
Fragments arranged by size:
3115 2368 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4568 844
Size: 3724 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3237 5100 1394
Size: 429 1414 1863 3507
Fragments arranged by size:
3507 1863 1414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5708 5882 65
Size: 1183 4460 174 1396
Fragments arranged by size:
4460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3201 4396 5857 6044 1700
Size: 110 1391 1195 1461 187 2869
Fragments arranged by size:
2869 1461 1391 1195 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7213
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7213
BssSI C'ACGA_G
Cuts at: 3257 5273 6285 3257
Size: 2016 1012 4185
Fragments arranged by size:
4185 2016 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4409 794
Size: 3615 3598
BstBI TT'CG_AA
Cuts at: 2007 6017 2007
Size: 4010 3203
BstEII G'GTnAC_C
Cuts at: 1184 4167 1184
Size: 2983 4230
BstXI CCAn_nnnn'nTGG
Cuts at: 3881 3881
Size: 7213
BstYI r'GATC_y
Cuts at: 1972 2367 4543 4910 5295 5312 6764 6850 6862
Size: 395 2176 367 385 17 1452 86 12
Cuts at: 6862 7208 1972
Size: 346 1977
Fragments arranged by size:
2176 1977 1452 395 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2967 3531 3714 3937 4294 4345 513
Size: 552 1902 564 183 223 357 51 3381
Fragments arranged by size:
3381 1902 564 552 357 223 183 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3651 487
Size: 683 56 2425 4049
Fragments arranged by size:
4049 2425 683 56
BtsI GCAGTG_nn'
Cuts at: 3660 4729 5523 5543 3660
Size: 1069 794 20 5330
Fragments arranged by size:
5330 1069 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7178
DraI TTT'AAA
Cuts at: 3838 4517 3838
Size: 679 6534
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2462 6220 809
Size: 507 1146 3758 1802
Fragments arranged by size:
3758 1802 1146 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3203 3216 3614
Size: 54 1425 50 275 18 1373 13 398
Cuts at: 3614 4398 4660 5549 7178 8
Size: 784 262 889 1629 43
Fragments arranged by size:
1629 1425 1373 889 784 398 275 262
54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3203 4398 4660 1537
Size: 275 1391 1195 262 4090
Fragments arranged by size:
4090 1391 1195 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 2663 5143 1353
Size: 1310 2480 3423
Fragments arranged by size:
3423 2480 1310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5800 6174 6320 387
Size: 1064 138 4211 374 146 1280
Fragments arranged by size:
4211 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 2188
Size: 7213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 3032 3137 4434 5276 5873 6660
Size: 393 683 717 105 1297 842 597 787
Cuts at: 6660 6928 1239
Size: 268 1524
Fragments arranged by size:
1524 1297 842 787 717 683 597 393
268 105
EcoNI CCTnn'n_nnAGG
Cuts at: 3005 3005
Size: 7213
EcoO109I rG'GnC_Cy
Cuts at: 2353 2395 2644 2666 3584 3628 4439 4440 2353
Size: 42 249 22 918 44 811 1 5126
Fragments arranged by size:
5126 918 811 249 44 42 22 1
EcoRI G'AATT_C
Cuts at: 2026 4522 4900 2026
Size: 2496 378 4339
Fragments arranged by size:
4339 2496 378
EcoRV GAT'ATC
Cuts at: 2881 3866 4427 2881
Size: 985 561 5667
Fragments arranged by size:
5667 985 561
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 3145 2794
Size: 351 6862
FseI GG_CCGG'CC
Cuts at: 3205 3205
Size: 7213
FspI TGC'GCA
Cuts at: 5719 5719
Size: 7213
HaeII r_GCGC'y
Cuts at: 1678 3245 3711 3728 6360 1678
Size: 1567 466 17 2632 2531
Fragments arranged by size:
2632 2531 1567 466 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2331 2363 5854 5886 5928
Size: 32 135 32 759 32 3491 32 42
Cuts at: 5928 5960 1373
Size: 32 2626
Fragments arranged by size:
3491 2626 759 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2467 4132 4557 4803 669
Size: 988 281 529 1665 425 246 3079
Fragments arranged by size:
3079 1665 988 529 425 281 246
HindIII A'AGCT_T
Cuts at: 748 2765 3347 4389 748
Size: 2017 582 1042 3572
Fragments arranged by size:
3572 2017 1042 582
HpaI GTT'AAC
Cuts at: 4803 4803
Size: 7213
KasI G'GCGC_C
Cuts at: 3241 3241
Size: 7213
KpnI G_GTAC'C
Cuts at: 4538 4538
Size: 7213
MfeI C'AATT_G
Cuts at: 4812 4812
Size: 7213
MluI A'CGCG_T
Cuts at: 7089 7089
Size: 7213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3846 6327 6511 1305
Size: 2541 2481 184 2007
Fragments arranged by size:
2541 2481 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3218 10
Size: 54 1425 343 1386 4005
Fragments arranged by size:
4005 1425 1386 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 5171 5530 5689 518
Size: 648 1452 2553 359 159 2042
Fragments arranged by size:
2553 2042 1452 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3214 3222 3425 3705 4629 5306 6454 6699
Size: 1608 8 203 280 924 677 1148 245
Cuts at: 6699 1606
Size: 2120
Fragments arranged by size:
2120 1608 1148 924 677 280 245 203
8
NaeI GCC'GGC
Cuts at: 3203 3203
Size: 7213
NarI GG'CG_CC
Cuts at: 3242 3242
Size: 7213
NcoI C'CATG_G
Cuts at: 513 1065 2967 3531 4345 513
Size: 552 1902 564 814 3381
Fragments arranged by size:
3381 1902 814 564 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7213
NgoMIV G'CCGG_C
Cuts at: 3201 3201
Size: 7213
NheI G'CTAG_C
Cuts at: 1052 3898 4289 1052
Size: 2846 391 3976
Fragments arranged by size:
3976 2846 391
NotI GC'GGCC_GC
Cuts at: 4660 4660
Size: 7213
NsiI A_TGCA'T
Cuts at: 2685 2685
Size: 7213
NspI r_CATG'y
Cuts at: 1132 1229 2678 3761 4571 6116 1132
Size: 97 1449 1083 810 1545 2229
Fragments arranged by size:
2229 1545 1449 1083 810 97
PasI CC'CwG_GG
Cuts at: 1348 4001 1348
Size: 2653 4560
PciI A'CATG_T
Cuts at: 6112 6112
Size: 7213
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3098 4178 1108
Size: 1990 1080 4143
Fragments arranged by size:
4143 1990 1080
PfoI T'CCnGG_A
Cuts at: 4022 4022
Size: 7213
PmeI GTTT'AAAC
Cuts at: 4517 4517
Size: 7213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2889 2921 5282 5314 6795 6827 2889
Size: 32 2361 32 1481 32 3275
Fragments arranged by size:
3275 2361 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 3584 2395
Size: 1189 6024
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7213
PsiI TTA'TAA
Cuts at: 2559 4783 2559
Size: 2224 4989
PspOMI G'GGCC_C
Cuts at: 2352 2643 4439 2352
Size: 291 1796 5126
Fragments arranged by size:
5126 1796 291
PstI C_TGCA'G
Cuts at: 830 4360 4565 830
Size: 3530 205 3478
Fragments arranged by size:
3530 3478 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7213
PvuII CAG'CTG
Cuts at: 3425 3425
Size: 7213
SacI G_AGCT'C
Cuts at: 2190 2190
Size: 7213
SalI G'TCGA_C
Cuts at: 1936 4555 1936
Size: 2619 4594
SbfI CC_TGCA'GG
Cuts at: 4565 4565
Size: 7213
ScaI AGT'ACT
Cuts at: 5461 7173 5461
Size: 1712 5501
SexAI A'CCwGG_T
Cuts at: 3097 3097
Size: 7213
SfcI C'TryA_G
Cuts at: 826 1045 3507 3916 4356 4365 4462 4561 5696
Size: 219 2462 409 440 9 97 99 1135
Cuts at: 5696 6377 6568 826
Size: 681 191 1471
Fragments arranged by size:
2462 1471 1135 681 440 409 219 191
99 97 9
SfoI GGC'GCC
Cuts at: 3243 3243
Size: 7213
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7213
SmaI CCC'GGG
Cuts at: 1872 4540 1872
Size: 2668 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2363 2716 2768 2787 3175 5317
Size: 197 926 420 353 52 19 388 2142
Cuts at: 5317 6218 6480 820
Size: 901 262 1553
Fragments arranged by size:
2142 1553 926 901 420 388 353 262
197 52 19
SnaBI TAC'GTA
Cuts at: 493 4510 5004 493
Size: 4017 494 2702
Fragments arranged by size:
4017 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7213
SphI G_CATG'C
Cuts at: 1132 3761 4571 1132
Size: 2629 810 3774
Fragments arranged by size:
3774 2629 810
SspI AAT'ATT
Cuts at: 5 52 5137 5
Size: 47 5085 2081
Fragments arranged by size:
5085 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7213
StyI C'CwwG_G
Cuts at: 513 1065 2120 2698 2967 3129 3531 4345 4652
Size: 552 1055 578 269 162 402 814 307
Cuts at: 4652 513
Size: 3074
Fragments arranged by size:
3074 1055 814 578 552 402 307 269
162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5240 5257 5410 5595 6040 325
Size: 1255 3660 17 153 185 445 1498
Fragments arranged by size:
3660 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3841 4096 5459
Size: 274 80 33 51 1443 1864 255 1363
Cuts at: 5459 7171 96
Size: 1712 138
Fragments arranged by size:
1864 1712 1443 1363 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 7173 7205 3248
Size: 32 3893 32 3256
Fragments arranged by size:
3893 3256 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7213
XbaI T'CTAG_A
Cuts at: 4549 4549
Size: 7213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3529 3529
Size: 7213
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7213
XmaI C'CCGG_G
Cuts at: 1870 4538 1870
Size: 2668 4545
XmnI GAAnn'nnTTC
Cuts at: 2298 2904 2988 5342 2298
Size: 606 84 2354 4169
Fragments arranged by size:
4169 2354 606 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3499 5021 276
Size: 53 83 186 936 1965 1522 2468
Fragments arranged by size:
2468 1965 1522 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BplI BsiWI BstZ17I
Bsu36I DraIII FspAI NruI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SfiI SrfI SwaI