Restriction map for
pFN21ASDA0014
(Circular) (Six-base) MAPSORT of: pFN21ASDA0014.seq Check: 378 from: 1 to: 6208
pFN21ASDA0014 6208 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2044 2044
Size: 6208
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3210 3529 3210
Size: 319 5889
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3478 4271 5655 5923 1632
Size: 1846 793 1384 268 1917
Fragments arranged by size:
1917 1846 1384 793 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2178 2749 5107 6084 1203
Size: 975 571 2358 977 1327
Fragments arranged by size:
2358 1327 977 975 571
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 3144 4937 3144
Size: 1793 4415
AleI CACnn'nnGTG
Cuts at: 1166 2033 1166
Size: 867 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2615 1184
Size: 1431 4777
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 6176
AlwNI CAG_nnn'CTG
Cuts at: 2059 2089 2212 2324 2495 2671 2770 3097 3951
Size: 30 123 112 171 176 99 327 854
Cuts at: 3951 5523 2059
Size: 1572 2744
Fragments arranged by size:
2744 1572 854 327 176 171 123 112
99 30
ApaLI G'TGCA_C
Cuts at: 3302 4265 5421 3302
Size: 963 1156 4089
Fragments arranged by size:
4089 1156 963
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 3533 1870
Size: 73 1590 4545
Fragments arranged by size:
4545 1590 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2165 2198 2165
Size: 33 6175
BamHI G'GATC_C
Cuts at: 2410 3538 3905 2410
Size: 1128 367 4713
Fragments arranged by size:
4713 1128 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 3082 3210 3529 4984
Size: 325 217 627 630 665 128 319 1455
Cuts at: 4984 618
Size: 1842
Fragments arranged by size:
1842 1455 665 630 627 325 319 217
128
BanII G_rGCy'C
Cuts at: 1391 2889 2900 2951 3020 3527 1391
Size: 1498 11 51 69 507 4072
Fragments arranged by size:
4072 1498 507 69 51 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 928
Size: 971 257 4980
Fragments arranged by size:
4980 971 257
BbvCI CC'TCA_GC
Cuts at: 2628 2628
Size: 6208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 2739 1548
Size: 1191 5017
BfrBI ATG'CAT
Cuts at: 2463 2463
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 2727 3225
Size: 107 122 71 631 605 412 643 498
Cuts at: 3225 4819 136
Size: 1594 1525
Fragments arranged by size:
1594 1525 643 631 605 498 412 122
107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 2888 2933 3285 3625 2888
Size: 45 352 340 5471
Fragments arranged by size:
5471 352 340 45
Bme1580I G_kGCm'C
Cuts at: 1790 2230 2245 3306 3445 4269 5425 1790
Size: 440 15 1061 139 824 1156 2573
Fragments arranged by size:
2573 1156 1061 824 440 139 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2975 2984 3152 3386 4868 1239
Size: 1736 9 168 234 1482 2579
Fragments arranged by size:
2579 1736 1482 234 168 9
Bpu10I CC'TnA_GC
Cuts at: 2628 2628
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2062 3020 3236 4333 5198 5496 2062
Size: 958 216 1097 865 298 2774
Fragments arranged by size:
2774 1097 958 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4871 882
Size: 617 3372 2219
Fragments arranged by size:
3372 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2181 2752 3505 3999 493
Size: 711 977 571 753 494 2702
Fragments arranged by size:
2702 977 753 711 571 494
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4015 4397 5032 275
Size: 53 83 186 936 2482 382 635 1451
Fragments arranged by size:
2482 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3207 4641 5039 5313 5460 5871 3207
Size: 1434 398 274 147 411 3544
Fragments arranged by size:
3544 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 1178
Size: 30 165 30 1131 30 4822
Fragments arranged by size:
4822 1131 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2890 3398 1186
Size: 1704 508 3996
Fragments arranged by size:
3996 1704 508
BseYI C'CCAG_C
Cuts at: 2469 3162 5411 5907 2469
Size: 693 2249 496 2770
Fragments arranged by size:
2770 2249 693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5635
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4419 5447 1540
Size: 275 186 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2048 2889 2983 3306 3453 4269 4354 5425 2048
Size: 841 94 323 147 816 85 1071 2831
Fragments arranged by size:
2831 1071 841 816 323 147 94 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2230 2245 2889 2900 2951 2983
Size: 399 258 182 15 644 11 51 32
Cuts at: 2983 3020 3306 3445 3453 3527 4269 4354 5425
Size: 37 286 139 8 74 742 85 1071
Cuts at: 5425 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 644 399 286 258 182
139 85 74 51 37 32 15 11
8
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 2541 2628 2643 2709 3563 844
Size: 1200 497 87 15 66 854 3489
Fragments arranged by size:
3489 1200 854 497 87 66 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2257 4095 1394
Size: 429 434 1838 3507
Fragments arranged by size:
3507 1838 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2463 4703 4877 65
Size: 1183 1215 2240 174 1396
Fragments arranged by size:
2240 1396 1215 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 2362 3421 794
Size: 1265 303 1059 3581
Fragments arranged by size:
3581 1265 1059 303
BstBI TT'CG_AA
Cuts at: 2007 5012 2007
Size: 3005 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2410 3538 3905 4290 4307 5759 5845 5857
Size: 438 1128 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1128 438 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2991 3433 2991
Size: 442 5766
BtgI C'CryG_G
Cuts at: 513 1065 2514 2820 3240 3459 513
Size: 552 1449 306 420 219 3262
Fragments arranged by size:
3262 1449 552 420 306 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2230 3724 4518 4538 2069
Size: 161 1494 794 20 3739
Fragments arranged by size:
3739 1494 794 161 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 2794 2794
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2772 3171 3341
Size: 54 1425 50 275 18 942 399 170
Cuts at: 3341 3655 4544 6173 8
Size: 314 889 1629 43
Fragments arranged by size:
1629 1425 942 889 399 314 275 170
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 4138 1353
Size: 764 2021 3423
Fragments arranged by size:
3423 2021 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2887 2887
Size: 6208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2975 2984 3152 3386 3478 4271 4868
Size: 393 1343 9 168 234 92 793 597
Cuts at: 4868 5655 5923 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1343 793 787 597 393 268 234
168 92 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2249 2567 3365 2249
Size: 318 798 5092
Fragments arranged by size:
5092 798 318
EcoO109I rG'GnC_Cy
Cuts at: 2086 2617 2632 2995 2086
Size: 531 15 363 5299
Fragments arranged by size:
5299 531 363 15
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 6208
FspI TGC'GCA
Cuts at: 2645 4714 2645
Size: 2069 4139
HaeII r_GCGC'y
Cuts at: 1678 5355 1678
Size: 3677 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3130 3162 4849 4881 4923
Size: 32 135 32 1558 32 1687 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2626 1687 1558 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 3214 3533 3214
Size: 319 5889
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 5322 5506 1305
Size: 1719 2298 184 2007
Fragments arranged by size:
2298 2007 1719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2774 3173 10
Size: 54 1425 343 942 399 3045
Fragments arranged by size:
3045 1425 942 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2426 2513 4166 4525 4684 518
Size: 648 867 393 87 1653 359 159 2042
Fragments arranged by size:
2042 1653 867 648 393 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2075 2194 2212 2599 2929 3079 3166 3421
Size: 469 119 18 387 330 150 87 255
Cuts at: 3421 3624 4301 5449 5694 1606
Size: 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 469 387 330 255 245
203 150 119 87 18
NcoI C'CATG_G
Cuts at: 513 1065 3240 513
Size: 552 2175 3481
Fragments arranged by size:
3481 2175 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NsiI A_TGCA'T
Cuts at: 2465 2465
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2278 2360 2431 2463 3026 3566 5111
Size: 97 1049 82 71 32 563 540 1545
Cuts at: 5111 1132
Size: 2229
Fragments arranged by size:
2229 1545 1049 563 540 97 82 71
32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2770 3169 1108
Size: 1662 399 4147
Fragments arranged by size:
4147 1662 399
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PmlI CAC'GTG
Cuts at: 2181 2752 2181
Size: 571 5637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2566 4277 4309 5790 5822 2534
Size: 32 1711 32 1481 32 2920
Fragments arranged by size:
2920 1711 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2617 2632 2995 2617
Size: 15 363 5830
Fragments arranged by size:
5830 363 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2064 2237 2253 2351 2667 2946 3560 830
Size: 1234 173 16 98 316 279 614 3478
Fragments arranged by size:
3478 1234 614 316 279 173 98 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2075 2212 2929 3079 3166 3421 2075
Size: 137 717 150 87 255 4862
Fragments arranged by size:
4862 717 255 150 137 87
SacI G_AGCT'C
Cuts at: 2889 2889
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 2253 3560 2253
Size: 1307 4901
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 2060 2216 2233 2249 2347 2663 2942
Size: 219 1015 156 17 16 98 316 279
Cuts at: 2942 3556 4691 5372 5563 826
Size: 614 1135 681 191 1471
Fragments arranged by size:
1471 1135 1015 681 614 316 279 219
191 156 98 17 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 2727 2727
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 3035 3251 4312 5213 5475
Size: 197 926 98 994 216 1061 901 262
Cuts at: 5475 820
Size: 1553
Fragments arranged by size:
1553 1061 994 926 901 262 216 197
98
SnaBI TAC'GTA
Cuts at: 493 3505 3999 493
Size: 3012 494 2702
Fragments arranged by size:
3012 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 2278 2360 3566 1132
Size: 1146 82 1206 3774
Fragments arranged by size:
3774 1206 1146 82
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 3371 1517
Size: 1854 4354
StyI C'CwwG_G
Cuts at: 513 1065 3214 3240 3647 513
Size: 552 2149 26 407 3074
Fragments arranged by size:
3074 2149 552 407 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3176 4235 4252 4405 4590 5035 325
Size: 1255 1596 1059 17 153 185 445 1498
Fragments arranged by size:
1596 1498 1255 1059 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2438 4454 6166
Size: 274 80 33 51 1443 461 2016 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
2016 1712 1443 461 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3168 3200 6168 6200 3168
Size: 32 2968 32 3176
Fragments arranged by size:
3176 2968 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2477 2795 2477
Size: 318 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BplI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfoI SrfI SwaI