Restriction map for
pFN21AF0414
(Circular) (Six-base) MAPSORT of: pFN21AF0414.seq Check: 2053 from: 1 to: 7879
pFN21AF0414 7879 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2338 3484 5077 2338
Size: 1146 1593 5140
Fragments arranged by size:
5140 1593 1146
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2742 5689 278
Size: 53 83 186 936 1206 2947 2468
Fragments arranged by size:
2947 2468 1206 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5222 1937
Size: 3285 4594
Acc65I G'GTAC_C
Cuts at: 5200 5200
Size: 7879
AclI AA'CG_TT
Cuts at: 1563 6006 6379 1563
Size: 4443 373 3063
Fragments arranged by size:
4443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 3234 3335 3490 4093 5942 7326 7594
Size: 455 1147 101 155 603 1849 1384 268
Cuts at: 7594 1632
Size: 1917
Fragments arranged by size:
1917 1849 1384 1147 603 455 268 155
101
AfeI AGC'GCT
Cuts at: 2339 2339
Size: 7879
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7682
AflIII A'CryG_T
Cuts at: 1203 2616 4135 4137 6778 7755 1203
Size: 1413 1519 2 2641 977 1327
Fragments arranged by size:
2641 1519 1413 1327 977 2
AgeI A'CCGG_T
Cuts at: 6710 6710
Size: 7879
AhdI GACnn_n'nnGTC
Cuts at: 3226 6608 3226
Size: 3382 4497
AleI CACnn'nnGTG
Cuts at: 1166 2814 1166
Size: 1648 6231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4136 1184
Size: 2952 4927
AlwNI CAG_nnn'CTG
Cuts at: 2065 2137 2539 2848 3532 4219 5622 7194 2065
Size: 72 402 309 684 687 1403 1572 2750
Fragments arranged by size:
2750 1572 1403 687 684 402 309 72
ApaI G_GGCC'C
Cuts at: 2679 3421 4449 4485 4720 4741 4930 2679
Size: 742 1028 36 235 21 189 5628
Fragments arranged by size:
5628 1028 742 235 189 36 21
ApaLI G'TGCA_C
Cuts at: 2516 2696 4019 5936 7092 2516
Size: 180 1323 1917 1156 3303
Fragments arranged by size:
3303 1917 1323 1156 180
ApoI r'AATT_y
Cuts at: 1493 5188 5414 5566 7675 1493
Size: 3695 226 152 2109 1697
Fragments arranged by size:
3695 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6433 160
Size: 872 5401 1606
Fragments arranged by size:
5401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
AvaI C'yCGr_G
Cuts at: 1870 1943 2378 3361 3414 3803 4442 4500 4574
Size: 73 435 983 53 389 639 58 74
Cuts at: 4574 4672 4713 4719 4771 4870 5204 1870
Size: 98 41 6 52 99 334 4545
Fragments arranged by size:
4545 983 639 435 389 334 99 98
74 73 58 53 52 41 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4124 4157 4124
Size: 33 7846
BamHI G'GATC_C
Cuts at: 4317 4619 5209 5576 4317
Size: 302 590 367 6620
Fragments arranged by size:
6620 590 367 302
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4435 4510 4907 5200 6655
Size: 325 217 627 2648 75 397 293 1455
Cuts at: 6655 618
Size: 1842
Fragments arranged by size:
2648 1842 1455 627 397 325 293 217
75
BanII G_rGCy'C
Cuts at: 1391 2480 2679 2691 3421 3933 4449 4485 4538
Size: 1089 199 12 730 512 516 36 53
Cuts at: 4538 4720 4741 4930 5198 1391
Size: 182 21 189 268 4072
Fragments arranged by size:
4072 1089 730 516 512 268 199 189
182 53 36 21 12
BbeI G_GCGC'C
Cuts at: 4514 4514
Size: 7879
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3404 928
Size: 971 1505 5403
Fragments arranged by size:
5403 1505 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2926 2960 6070 6104 1903
Size: 34 989 34 3110 34 3678
Fragments arranged by size:
3678 3110 989 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5136 5771 6981 5136
Size: 635 1210 6034
Fragments arranged by size:
6034 1210 635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7879
BfrBI ATG'CAT
Cuts at: 3062 4602 3062
Size: 1540 6339
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4230 4371 4444
Size: 107 122 71 631 605 2558 141 73
Cuts at: 4444 6490 136
Size: 2046 1525
Fragments arranged by size:
2558 2046 1525 631 605 141 122 107
73 71
BglII A'GATC_T
Cuts at: 7874 7874
Size: 7879
BlpI GC'TnA_GC
Cuts at: 2540 5296 2540
Size: 2756 5123
Bme1580I G_kGCm'C
Cuts at: 1790 2520 2679 2700 3421 4023 4440 4449 4485
Size: 730 159 21 721 602 417 9 36
Cuts at: 4485 4720 4741 4930 5940 7096 1790
Size: 235 21 189 1010 1156 2573
Fragments arranged by size:
2573 1156 1010 730 721 602 417 235
189 159 36 21 21 9
BmgBI CAC'GTC
Cuts at: 1318 3223 1318
Size: 1905 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2596 2780 2836
Size: 416 53 83 399 24 1179 184 56
Cuts at: 2836 4742 5171 5610 6568 442
Size: 1906 429 439 958 1753
Fragments arranged by size:
1906 1753 1179 958 439 429 416 399
184 83 56 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3271 3598 4831 4837 4988 6539 1239
Size: 2032 327 1233 6 151 1551 2579
Fragments arranged by size:
2579 2032 1551 1233 327 151 6
Bpu10I CC'TnA_GC
Cuts at: 2111 2502 2111
Size: 391 7488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3731 3797 6004 6869 7167 2915
Size: 816 66 2207 865 298 3627
Fragments arranged by size:
3627 2207 865 816 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3506 3995 6542 882
Size: 617 2007 489 2547 2219
Fragments arranged by size:
2547 2219 2007 617 489
BsaAI yAC'GTr
Cuts at: 493 1204 3742 4138 5670 493
Size: 711 2538 396 1532 2702
Fragments arranged by size:
2702 2538 1532 711 396
BsaBI GATnn'nnATC
Cuts at: 7873 7873
Size: 7879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 3482 4511 5686
Size: 53 83 186 936 1206 743 1029 1175
Cuts at: 5686 6068 6703 275
Size: 382 635 1451
Fragments arranged by size:
1451 1206 1175 1029 936 743 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3831 4459 6312 6710 6984 7131 7542 3831
Size: 628 1853 398 274 147 411 4168
Fragments arranged by size:
4168 1853 628 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3146 3176 3467 3497 3581
Size: 30 165 30 1743 30 291 30 84
Cuts at: 3581 3611 4394 4424 4804 4834 1178
Size: 30 783 30 380 30 4223
Fragments arranged by size:
4223 1743 783 380 291 165 84 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3038 3350 3586 4013 4985 1186
Size: 1852 312 236 427 972 4080
Fragments arranged by size:
4080 1852 972 427 312 236
BseYI C'CCAG_C
Cuts at: 2221 2443 2667 2965 3877 4280 4468 4583 4667
Size: 222 224 298 912 403 188 115 84
Cuts at: 4667 5054 7082 7578 2221
Size: 387 2028 496 2522
Fragments arranged by size:
2522 2028 912 496 403 387 298 224
222 188 115 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 6919
Fragments arranged by size:
6919 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5329 6090 7118 1540
Size: 275 186 3328 761 1028 2301
Fragments arranged by size:
3328 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2520 2700 3831 4023 5109 5115 5940 6025
Size: 12 180 1131 192 1086 6 825 85
Cuts at: 6025 7096 2508
Size: 1071 3291
Fragments arranged by size:
3291 1131 1086 1071 825 192 180 85
12 6
BsmI GAATG_Cn'
Cuts at: 5390 5483 5390
Size: 93 7786
BsmBI CGTCTCn'nnnn_
Cuts at: 3172 3172
Size: 7879
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2480 2508 2520 2679 2691 2700 3421
Size: 399 690 28 12 159 12 9 721
Cuts at: 3421 3831 3933 4023 4440 4449 4485 4538 4720
Size: 410 102 90 417 9 36 53 182
Cuts at: 4720 4741 4930 5109 5115 5198 5940 6025 7096
Size: 21 189 179 6 83 742 85 1071
Cuts at: 7096 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 721 690 417 410 399
189 182 179 159 102 90 85 83
53 36 28 21 12 12 9 9
6
BspEI T'CCGG_A
Cuts at: 3831 4459 7542 3831
Size: 628 3083 4168
Fragments arranged by size:
4168 3083 628
BspHI T'CATG_A
Cuts at: 5768 7498 5768
Size: 1730 6149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2289 2338 3484 4204 4462 5077 5234
Size: 1322 123 49 1146 720 258 615 157
Cuts at: 5234 844
Size: 3489
Fragments arranged by size:
3489 1322 1146 720 615 258 157 123
49
BsrBI CCG'CTC
Cuts at: 1394 1823 4660 5766 1394
Size: 429 2837 1106 3507
Fragments arranged by size:
3507 2837 1106 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3265 6374 6548 65
Size: 1183 2017 3109 174 1396
Fragments arranged by size:
3109 2017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 3544 3647 4628 5003 6523 6710
Size: 110 199 1535 103 981 375 1520 187
Cuts at: 6710 1700
Size: 2869
Fragments arranged by size:
2869 1535 1520 981 375 199 187 110
103
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7879
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7879
BssSI C'ACGA_G
Cuts at: 2579 2945 5100 5108 5114 5939 6951 2579
Size: 366 2155 8 6 825 1012 3507
Fragments arranged by size:
3507 2155 1012 825 366 8 6
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2137 3826 794
Size: 1343 1689 4847
Fragments arranged by size:
4847 1689 1343
BstBI TT'CG_AA
Cuts at: 6683 6683
Size: 7879
BstEII G'GTnAC_C
Cuts at: 1184 2585 5064 1184
Size: 1401 2479 3999
Fragments arranged by size:
3999 2479 1401
BstXI CCAn_nnnn'nTGG
Cuts at: 3451 4420 3451
Size: 969 6910
BstYI r'GATC_y
Cuts at: 1972 2724 2940 4317 4619 5209 5576 5961 5978
Size: 752 216 1377 302 590 367 385 17
Cuts at: 5978 7430 7516 7528 7874 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1377 752 590 385 367 346
302 216 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4902 4902
Size: 7879
BtgI C'CryG_G
Cuts at: 513 1065 2004 2701 3450 3886 5129 5143 513
Size: 552 939 697 749 436 1243 14 3249
Fragments arranged by size:
3249 1243 939 749 697 552 436 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7140
Fragments arranged by size:
7140 683 56
BtsI GCAGTG_nn'
Cuts at: 2809 2821 2899 3090 5395 6189 6209 2809
Size: 12 78 191 2305 794 20 4479
Fragments arranged by size:
4479 2305 794 191 78 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7844
DraI TTT'AAA
Cuts at: 5183 5183
Size: 7879
DraIII CAC_nnn'GTG
Cuts at: 2515 2515
Size: 7879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6886 809
Size: 507 5570 1802
Fragments arranged by size:
5570 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3262 3324
Size: 54 1425 50 275 18 177 1255 62
Cuts at: 3324 3453 3844 4090 4332 4590 4661 5326 6215
Size: 129 391 246 242 258 71 665 889
Cuts at: 6215 7844 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1255 889 665 391 275 258
246 242 177 129 71 62 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5326 1537
Size: 275 3514 4090
Fragments arranged by size:
4090 3514 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2735 3210 4152 4241 4526 5809 1353
Size: 873 509 475 942 89 285 1283 3423
Fragments arranged by size:
3423 1283 942 873 509 475 285 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3703 4990 6466 6840 6986 387
Size: 1064 138 2114 1287 1476 374 146 1280
Fragments arranged by size:
2114 1476 1287 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 3234 3271 3335 3490 3598 4093
Size: 393 455 1147 37 64 155 108 495
Cuts at: 4093 4831 4837 4988 5942 6539 7326 7594 1239
Size: 738 6 151 954 597 787 268 1524
Fragments arranged by size:
1524 1147 954 787 738 597 495 455
393 268 155 151 108 64 37 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2948 2948
Size: 7879
EcoO109I rG'GnC_Cy
Cuts at: 2125 2272 2629 3148 3418 3793 4481 4645 4676
Size: 147 357 519 270 375 688 164 31
Cuts at: 4676 4737 4738 4789 4799 4806 4887 4926 4927
Size: 61 1 51 10 7 81 39 1
Cuts at: 4927 2125
Size: 5077
Fragments arranged by size:
5077 688 519 375 357 270 164 147
81 61 51 39 31 10 7 1
1
EcoRI G'AATT_C
Cuts at: 5188 5566 5188
Size: 378 7501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 5032 2746
Size: 2286 5593
FspI TGC'GCA
Cuts at: 6385 6385
Size: 7879
HaeII r_GCGC'y
Cuts at: 1678 2341 3723 4341 4514 7026 1678
Size: 663 1382 618 173 2512 2531
Fragments arranged by size:
2531 2512 1382 663 618 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2867 2899 3212 3244 3467
Size: 32 135 32 1295 32 313 32 223
Cuts at: 3467 3499 4394 4426 4804 4836 6520 6552 6594
Size: 32 895 32 378 32 1684 32 42
Cuts at: 6594 6626 1373
Size: 32 2626
Fragments arranged by size:
2626 1684 1295 895 378 313 223 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5223 5469 669
Size: 988 281 3285 246 3079
Fragments arranged by size:
3285 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3280 748
Size: 2532 5347
HpaI GTT'AAC
Cuts at: 5469 5469
Size: 7879
KasI G'GCGC_C
Cuts at: 4510 4510
Size: 7879
KpnI G_GTAC'C
Cuts at: 5204 5204
Size: 7879
MfeI C'AATT_G
Cuts at: 5478 5478
Size: 7879
MluI A'CGCG_T
Cuts at: 7755 7755
Size: 7879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6993 7177 1305
Size: 5688 184 2007
Fragments arranged by size:
5688 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3455 3846 4092 10
Size: 54 1425 343 1623 391 246 3797
Fragments arranged by size:
3797 1623 1425 391 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 2511 2814 3470 4172 4601 5837
Size: 648 1153 192 303 656 702 429 1236
Cuts at: 5837 6196 6355 518
Size: 359 159 2042
Fragments arranged by size:
2042 1236 1153 702 656 648 429 359
303 192 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2539 2649 3489 3700 3823 4200 4315
Size: 528 405 110 840 211 123 377 115
Cuts at: 4315 4491 4517 4896 5295 5972 7120 7365 1606
Size: 176 26 379 399 677 1148 245 2120
Fragments arranged by size:
2120 1148 840 677 528 405 399 379
377 245 211 176 123 115 110 26
NaeI GCC'GGC
Cuts at: 2011 3546 3649 4630 2011
Size: 1535 103 981 5260
Fragments arranged by size:
5260 1535 981 103
NarI GG'CG_CC
Cuts at: 4511 4511
Size: 7879
NcoI C'CATG_G
Cuts at: 513 1065 2004 2701 513
Size: 552 939 697 5691
Fragments arranged by size:
5691 939 697 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7879
NgoMIV G'CCGG_C
Cuts at: 2009 3544 3647 4628 2009
Size: 1535 103 981 5260
Fragments arranged by size:
5260 1535 981 103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7879
NotI GC'GGCC_GC
Cuts at: 5326 5326
Size: 7879
NsiI A_TGCA'T
Cuts at: 3064 4604 3064
Size: 1540 6339
NspI r_CATG'y
Cuts at: 1132 1229 2620 3763 4027 4181 4602 5237 6782
Size: 97 1391 1143 264 154 421 635 1545
Cuts at: 6782 1132
Size: 2229
Fragments arranged by size:
2229 1545 1391 1143 635 421 264 154
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7879
PciI A'CATG_T
Cuts at: 2616 6778 2616
Size: 4162 3717
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7879
PfoI T'CCnGG_A
Cuts at: 4377 4563 4377
Size: 186 7693
PmeI GTTT'AAAC
Cuts at: 5183 5183
Size: 7879
PmlI CAC'GTG
Cuts at: 4138 4138
Size: 7879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5948 5980 7461 7493 5948
Size: 32 1481 32 6334
Fragments arranged by size:
6334 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2272 2629 3148 3793 4799 4887 2125
Size: 147 357 519 645 1006 88 5117
Fragments arranged by size:
5117 1006 645 519 357 147 88
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7879
PsiI TTA'TAA
Cuts at: 5449 5449
Size: 7879
PspOMI G'GGCC_C
Cuts at: 2675 3417 4445 4481 4716 4737 4926 2675
Size: 742 1028 36 235 21 189 5628
Fragments arranged by size:
5628 1028 742 235 189 36 21
PstI C_TGCA'G
Cuts at: 830 2133 2337 2349 2637 2816 3537 3699 3947
Size: 1303 204 12 288 179 721 162 248
Cuts at: 3947 4653 4916 4935 5231 830
Size: 706 263 19 296 3478
Fragments arranged by size:
3478 1303 721 706 296 288 263 248
204 179 162 19 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7879
PvuII CAG'CTG
Cuts at: 2134 3489 3700 3823 4517 2134
Size: 1355 211 123 694 5496
Fragments arranged by size:
5496 1355 694 211 123
SalI G'TCGA_C
Cuts at: 1936 5221 1936
Size: 3285 4594
SapI GCTCTTCn'nnn_
Cuts at: 4526 4526
Size: 7879
SbfI CC_TGCA'GG
Cuts at: 2349 2637 4916 4935 5231 2349
Size: 288 2279 19 296 4997
Fragments arranged by size:
4997 2279 296 288 19
ScaI AGT'ACT
Cuts at: 6127 7839 6127
Size: 1712 6167
SexAI A'CCwGG_T
Cuts at: 3429 3429
Size: 7879
SfcI C'TryA_G
Cuts at: 826 1045 2129 2296 2333 2345 2633 2812 3533
Size: 219 1084 167 37 12 288 179 721
Cuts at: 3533 3695 3943 4129 4649 4912 4931 5227 6362
Size: 162 248 186 520 263 19 296 1135
Cuts at: 6362 7043 7234 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1084 721 681 520 296 288
263 248 219 191 186 179 167 162
37 19 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 4230 4230
Size: 7879
SfoI GGC'GCC
Cuts at: 4512 4512
Size: 7879
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
SgrAI Cr'CCGG_yG
Cuts at: 1700 4628 1700
Size: 2928 4951
SmaI CCC'GGG
Cuts at: 1872 3363 3416 3805 4444 4576 4674 4715 4721
Size: 1491 53 389 639 132 98 41 6
Cuts at: 4721 4773 4872 5206 1872
Size: 52 99 334 4545
Fragments arranged by size:
4545 1491 639 389 334 132 99 98
53 52 41 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 2930 3746 3812 5983 6884
Size: 197 926 435 552 816 66 2171 901
Cuts at: 6884 7146 820
Size: 262 1553
Fragments arranged by size:
2171 1553 926 901 816 552 435 262
197 66
SnaBI TAC'GTA
Cuts at: 493 3742 5670 493
Size: 3249 1928 2702
Fragments arranged by size:
3249 2702 1928
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7879
SphI G_CATG'C
Cuts at: 1132 5237 1132
Size: 4105 3774
SrfI GCCC'GGGC
Cuts at: 3805 4721 4872 3805
Size: 916 151 6812
Fragments arranged by size:
6812 916 151
SspI AAT'ATT
Cuts at: 5 52 5803 5
Size: 47 5751 2081
Fragments arranged by size:
5751 2081 47
StuI AGG'CCT
Cuts at: 1517 4163 4233 1517
Size: 2646 70 5163
Fragments arranged by size:
5163 2646 70
StyI C'CwwG_G
Cuts at: 513 1065 2004 2212 2701 2860 3516 4413 4794
Size: 552 939 208 489 159 656 897 381
Cuts at: 4794 5159 5318 513
Size: 365 159 3074
Fragments arranged by size:
3074 939 897 656 552 489 381 365
208 159 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 3473 3744 4681 5906 5923 6076
Size: 1255 993 900 271 937 1225 17 153
Cuts at: 6076 6261 6706 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1225 993 937 900 445 271
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3438 6125 7837
Size: 274 80 33 51 1443 1461 2687 1712
Cuts at: 7837 96
Size: 138
Fragments arranged by size:
2687 1712 1461 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3826 3858 7839 7871 3826
Size: 32 3981 32 3834
Fragments arranged by size:
3981 3834 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5092 1316
Size: 3776 4103
XbaI T'CTAG_A
Cuts at: 5215 5215
Size: 7879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4070 4070
Size: 7879
XhoI C'TCGA_G
Cuts at: 1943 2378 1943
Size: 435 7444
XmaI C'CCGG_G
Cuts at: 1870 3361 3414 3803 4442 4574 4672 4713 4719
Size: 1491 53 389 639 132 98 41 6
Cuts at: 4719 4771 4870 5204 1870
Size: 52 99 334 4545
Fragments arranged by size:
4545 1491 639 389 334 132 99 98
53 52 41 6
XmnI GAAnn'nnTTC
Cuts at: 6008 6008
Size: 7879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2740 5687 276
Size: 53 83 186 936 1206 2947 2468
Fragments arranged by size:
2947 2468 1206 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BplI BsiWI BstZ17I EcoICRI
EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII
SacI SacII SanDI SwaI