Restriction map for
pFN21AF0405
(Circular) (Six-base) MAPSORT of: pFN21AF0405.seq Check: 8545 from: 1 to: 5911
pFN21AF0405 5911 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2582 2582
Size: 5911
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3254 1937
Size: 436 881 4594
Fragments arranged by size:
4594 881 436
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 2101 4038 4411 1563
Size: 538 1937 373 3063
Fragments arranged by size:
3063 1937 538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 2329 2565 2645 3974 5358 5626 1632
Size: 660 37 236 80 1329 1384 268 1917
Fragments arranged by size:
1917 1384 1329 660 268 236 80 37
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 2623 3654 5226 2623
Size: 1031 1572 3308
Fragments arranged by size:
3308 1572 1031
ApaLI G'TGCA_C
Cuts at: 2381 3968 5124 2381
Size: 1587 1156 3168
Fragments arranged by size:
3168 1587 1156
ApoI r'AATT_y
Cuts at: 1493 2725 3220 3446 3598 5707 1493
Size: 1232 495 226 152 2109 1697
Fragments arranged by size:
2109 1697 1232 495 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 3236 1870
Size: 73 1293 4545
Fragments arranged by size:
4545 1293 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3232 4687 618
Size: 325 217 627 1445 1455 1842
Fragments arranged by size:
1842 1455 1445 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 3230 1391
Size: 624 1215 4072
Fragments arranged by size:
4072 1215 624
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 3006 928
Size: 971 636 471 3833
Fragments arranged by size:
3833 971 636 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2341 2375 4102 4136 1903
Size: 34 404 34 1727 34 3678
Fragments arranged by size:
3678 1727 404 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BfrBI ATG'CAT
Cuts at: 2195 2954 2195
Size: 759 5152
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2804 4522 136
Size: 107 122 71 631 605 1132 1718 1525
Fragments arranged by size:
1718 1525 1132 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2385 3972 5128 1790
Size: 595 1587 1156 2573
Fragments arranged by size:
2573 1587 1156 595
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4600 442
Size: 416 53 83 399 24 2225 958 1753
Fragments arranged by size:
2225 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2133 2133
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2482 2489 4571 1239
Size: 1243 7 2082 2579
Fragments arranged by size:
2579 2082 1243 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4574 882
Size: 617 3075 2219
Fragments arranged by size:
3075 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 2803 3702 493
Size: 711 1530 69 899 2702
Fragments arranged by size:
2702 1530 899 711 69
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3718 4100 4735 275
Size: 53 83 186 936 2185 382 635 1451
Fragments arranged by size:
2185 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2749 2779 2849 2879 1178
Size: 30 165 30 1346 30 70 30 4210
Fragments arranged by size:
4210 1346 165 70 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2751 2754 2757 1186
Size: 1565 3 3 4340
Fragments arranged by size:
4340 1565 3 3
BseYI C'CCAG_C
Cuts at: 2282 3197 5114 5610 2282
Size: 915 1917 496 2583
Fragments arranged by size:
2583 1917 915 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2439 1163
Size: 573 703 4635
Fragments arranged by size:
4635 703 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 3972 4057 5128 2385
Size: 1587 85 1071 3168
Fragments arranged by size:
3168 1587 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3422 3515 3143
Size: 279 93 5539
Fragments arranged by size:
5539 279 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2046 2046
Size: 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2385 3230 3972 4057 5128 1391
Size: 399 225 370 845 742 85 1071 2174
Fragments arranged by size:
2174 1071 845 742 399 370 225 85
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 2770 3800 5530 2770
Size: 1030 1730 3151
Fragments arranged by size:
3151 1730 1030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 2582 3266 844
Size: 1387 351 684 3489
Fragments arranged by size:
3489 1387 684 351
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4555 4742 1700
Size: 110 2745 187 2869
Fragments arranged by size:
2869 2745 187 110
BsrGI T'GTAC_A
Cuts at: 96 3031 96
Size: 2935 2976
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5911
BstBI TT'CG_AA
Cuts at: 2242 4715 2242
Size: 2473 3438
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2681 2681
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2493 3241 3608 3993 4010 5462 5548 5560
Size: 521 748 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 748 521 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3019 513
Size: 552 1954 3405
Fragments arranged by size:
3405 1954 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3140 487
Size: 683 56 1914 3258
Fragments arranged by size:
3258 1914 683 56
BtsI GCAGTG_nn'
Cuts at: 2427 3427 4221 4241 2427
Size: 1000 794 20 4097
Fragments arranged by size:
4097 1000 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 2413 2635 3215 2413
Size: 222 580 5109
Fragments arranged by size:
5109 580 222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3358 4247
Size: 54 1425 50 275 18 1192 336 889
Cuts at: 4247 5876 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1192 889 336 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2348 3841 1353
Size: 918 77 1493 3423
Fragments arranged by size:
3423 1493 918 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2329 2482 2489 2565 2645 3974
Size: 393 660 37 153 7 76 80 1329
Cuts at: 3974 4571 5358 5626 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1329 787 660 597 393 268 153
80 76 37 7
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 5058 1678
Size: 3380 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2849 2881 4552 4584 4626
Size: 32 135 32 1277 32 1671 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 1671 1277 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2662 5025 5209 1305
Size: 1357 2363 184 2007
Fragments arranged by size:
2363 2007 1357 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4089
Fragments arranged by size:
4089 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3869 4228 4387 518
Size: 648 2703 359 159 2042
Fragments arranged by size:
2703 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2439 3201 3327 4004 5152 5397 1606
Size: 833 762 126 677 1148 245 2120
Fragments arranged by size:
2120 1148 833 762 677 245 126
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NruI TCG'CGA
Cuts at: 3159 3159
Size: 5911
NsiI A_TGCA'T
Cuts at: 2197 2956 2197
Size: 759 5152
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PfoI T'CCnGG_A
Cuts at: 2957 2957
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 3980 4012 5493 5525 2270
Size: 32 1678 32 1481 32 2656
Fragments arranged by size:
2656 1678 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2439 3201 2439
Size: 762 5149
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SapI GCTCTTCn'nnn_
Cuts at: 2348 2348
Size: 5911
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2884 3259 4394 5075 5266 826
Size: 219 1839 375 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 375 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5911
StyI C'CwwG_G
Cuts at: 513 1065 2874 3350 513
Size: 552 1809 476 3074
Fragments arranged by size:
3074 1809 552 476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2896 3938 3955 4108 4293 4738 325
Size: 1255 1316 1042 17 153 185 445 1498
Fragments arranged by size:
1498 1316 1255 1042 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 3031 4157
Size: 274 80 33 51 1443 255 799 1126
Cuts at: 4157 5869 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1126 799 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2711 2743 2914 2946 5871 5903 2711
Size: 32 171 32 2925 32 2719
Fragments arranged by size:
2925 2719 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2955 3032 2955
Size: 77 5834
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 2613 4040 2613
Size: 1427 4484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI